Efeitos da privação de sono em parâmetros de recuperação muscular, após dano induzido por ação excêntrica

• Autor(a) principal: Dáttilo, Murilo [UNIFESP]
• Data de Publicação: 2015
• Tipo de documento: Tese
• Idioma: por
• Título da fonte: Repositório Institucional da UNIFESP
• Texto Completo: https://sucupira.capes.gov.br/sucupira/public/consultas/coleta/trabalhoConclusao/viewTrabalhoConclusao.jsf?popup=true&id_trabalho=2688482; http://repositorio.unifesp.br/handle/11600/48839
• topic: privação de sono; dano muscular; recuperação muscular; hormônios; citocinas

Introduction: Popularly, sleep is known as an essential issue to maintain muscle physiology, as well as for recovery of its functions after various types of damage, as well as those seen after bouts of exercise. However, it is not known how some variables related to muscle recovery respond under sleep deprivation. Aim: To evaluate if total sleep deprivation for 60 hours, after muscle damage induced by eccentric action, modify de response of some parameters related to muscle recovery, such as: isometric strength, thigh girth, pain perception, pro- and antiinflammatory cytokines, and hormones. Materials and methods: In two separate conditions, with cross-over model and washout of at least four weeks, ten men (age = 24.5 ± 2.9 years; body mass index = 22.7 ± 2.3 kg / m2) performed a unilateral muscle damage protocol for lower limb, comprising 240 eccentric contractions for knee extensor muscles, performed in the isokinetic dynamometer. In the first condition, muscle damage protocol was followed by 60 hours of total sleep deprivation + 12 hours of normal sleep (2 sleep deprivation nights + 1 sleep night - Condition PS). In the second condition, the same protocol for muscle damage was conducted, but followed by 3 nights of normal sleep (Condition S). Blood samples were collected serially throughout the protocol for: creatine kinase (CK), testosterone (T), IGF-1, cortisol (C), tumor necrosis factor (TNF)-alpha, interleukin (IL)-1 beta, IL- 6 receptor antagonist of IL-1 (IL-1ra) and IL-10. After that, it was calculated the 24 h area under the curve (AUC) between hours 24 and 48 after the muscle damage protocol). Results: Peak torque reduced at all times after muscle damage in both conditions (F (4,72) = 70.57, p < 0.001). At hour 36, Condition PS showed lower strength compared to Condition S (p = 0.81, d = 1.3). For serum CK, there was only effect of time (F (5,90) = 17.6, p <0.001), with higher values being observed at hour 60 compared to baseline (p < 0.05). The thigh perimeter did not show significant differences between and within conditions. However, in both conditions, all moments after muscle damage were showed by high effect size in comparison to baseline (d > 1.0) . The pain perception showed only effect of time (F (4,56) = 17.3, p < 0.001), with all moments being higher than baseline, in both conditions (p < 0.05 ). Through the effect size analysis, Condition PS showed higher pain perception at hours 36 (p = 0.9, d = 0.8) and 60 (p = 0.99, d = 0.5). IL-6 and IL-1ra increased from immediately to 2 hours after muscle damage, in both conditions (p < 0.05). When 24-h AUC was analyzed, there was a tendency to increase in IL-6 in the Condition PS, with high effect size (t = -1.97, df = 8, p = 0.08, d = 0.8). The 24-hour AUC showed that IGF-1 (t = -2.76, df = 8, p = 0.02, d = 0.7) and cortisol (t = -7.69, df = 8, p < 0.001, d = 2.7) were higher in the Condition PS, with higher cortisol:testosterone ratio (t = -3.4, df = 8, p = 0.009, d = 1.6). Conclusion: It was concluded that total sleep deprivation during the first two days after muscle damage delays strength recovery and modify hormonal and inflammatory responses, marked by increased levels of cortisol, IGF- 1, and IL 6.