Gold Nanoparticles Used as Protein Scavengers Enhance Surface Plasmon Resonance Signal
Autor(a) principal: | |
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Data de Publicação: | 2017 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNIFESP |
Texto Completo: | https://repositorio.unifesp.br/handle/11600/54023 http://dx.doi.org/10.3390/s17122765 |
Resumo: | Although several researchers had reported on methodologies for surface plasmon resonance (SPR) signal amplification based on the use of nanoparticles (NPs), the majority addressed the sandwich technique and low protein concentration. In this work, a different approach for SPR signal enhancement based on the use of gold NPs was evaluated. The method was used in the detection of two lectins, peanut agglutinin (PNA) and concanavalin A (ConA). Gold NPs were functionalized with antibodies anti-PNA and anti-ConA, and these NPs were used as protein scavengers in a solution. After being incubated with solutions of PNA or ConA, the gold NPs coupled with the collected lectins were injected on the sensor containing the immobilized antibodies. The signal amplification provided by this method was compared to the signal amplification provided by the direct coupling of PNA and ConA to gold NPs. Furthermore, both methods, direct coupling and gold NPs as protein scavengers, were compared to the direct detection of PNA and ConA in solution. Compared to the analysis of free protein, the direct coupling of PNA and ConA to gold NPs resulted in a signal amplification of 10-40-fold and a 13-fold decrease of the limit of detection (LOD), whereas the use of gold NPs as protein scavengers resulted in an SPR signal 40-50-times higher and an LOD 64-times lower. |
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de Macedo, Erenildo Ferreira [UNIFESP]Ducatti Formaggio, Daniela Maria [UNIFESP]Santos, Nivia Salles [UNIFESP]Tada, Dayane Batista [UNIFESP]2020-07-02T18:52:21Z2020-07-02T18:52:21Z2017Sensors. Basel, v. 17, n. 12, p. -, 2017.1424-8220https://repositorio.unifesp.br/handle/11600/54023http://dx.doi.org/10.3390/s17122765WOS000423285800065.pdf10.3390/s17122765WOS:000423285800065Although several researchers had reported on methodologies for surface plasmon resonance (SPR) signal amplification based on the use of nanoparticles (NPs), the majority addressed the sandwich technique and low protein concentration. In this work, a different approach for SPR signal enhancement based on the use of gold NPs was evaluated. The method was used in the detection of two lectins, peanut agglutinin (PNA) and concanavalin A (ConA). Gold NPs were functionalized with antibodies anti-PNA and anti-ConA, and these NPs were used as protein scavengers in a solution. After being incubated with solutions of PNA or ConA, the gold NPs coupled with the collected lectins were injected on the sensor containing the immobilized antibodies. The signal amplification provided by this method was compared to the signal amplification provided by the direct coupling of PNA and ConA to gold NPs. Furthermore, both methods, direct coupling and gold NPs as protein scavengers, were compared to the direct detection of PNA and ConA in solution. Compared to the analysis of free protein, the direct coupling of PNA and ConA to gold NPs resulted in a signal amplification of 10-40-fold and a 13-fold decrease of the limit of detection (LOD), whereas the use of gold NPs as protein scavengers resulted in an SPR signal 40-50-times higher and an LOD 64-times lower.Fundacao de Amparo a Pesquisa do Estado de Sao Paulo (FAPESP) [2011/23895-8, 2017/01697-6]Univ Fed Sao Paulo, Inst Ciencia & Tecnol, Rua Talim 330, BR-12231280 Sao Jose Dos Campos, SP, BrazilUniv Fed Sao Paulo, Inst Ciencia & Tecnol, Rua Talim 330, BR-12231280 Sao Jose Dos Campos, SP, BrazilFAPESP [2011/23895-8, 2017/01697-6]Web of Science-engMdpi AgSensorssurface plasmon resonancegold nanoparticlessignal amplificationlectinsconcanavalin Apeanut agglutininGold Nanoparticles Used as Protein Scavengers Enhance Surface Plasmon Resonance Signalinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleBasel1712info:eu-repo/semantics/openAccessreponame:Repositório Institucional da UNIFESPinstname:Universidade Federal de São Paulo (UNIFESP)instacron:UNIFESPORIGINALWOS000423285800065.pdfapplication/pdf438015${dspace.ui.url}/bitstream/11600/54023/1/WOS000423285800065.pdf4bf00c1904524c69ecc608c164bd4b09MD51open accessTEXTWOS000423285800065.pdf.txtWOS000423285800065.pdf.txtExtracted texttext/plain49899${dspace.ui.url}/bitstream/11600/54023/8/WOS000423285800065.pdf.txtcb2397413117106b29df70569c10fc8aMD58open accessTHUMBNAILWOS000423285800065.pdf.jpgWOS000423285800065.pdf.jpgIM Thumbnailimage/jpeg6477${dspace.ui.url}/bitstream/11600/54023/10/WOS000423285800065.pdf.jpgbd9e1e8aeed16439ea45899abf5fedbcMD510open access11600/540232023-06-05 19:07:49.013open accessoai:repositorio.unifesp.br:11600/54023Repositório InstitucionalPUBhttp://www.repositorio.unifesp.br/oai/requestopendoar:34652023-06-05T22:07:49Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)false |
dc.title.en.fl_str_mv |
Gold Nanoparticles Used as Protein Scavengers Enhance Surface Plasmon Resonance Signal |
title |
Gold Nanoparticles Used as Protein Scavengers Enhance Surface Plasmon Resonance Signal |
spellingShingle |
Gold Nanoparticles Used as Protein Scavengers Enhance Surface Plasmon Resonance Signal de Macedo, Erenildo Ferreira [UNIFESP] surface plasmon resonance gold nanoparticles signal amplification lectins concanavalin A peanut agglutinin |
title_short |
Gold Nanoparticles Used as Protein Scavengers Enhance Surface Plasmon Resonance Signal |
title_full |
Gold Nanoparticles Used as Protein Scavengers Enhance Surface Plasmon Resonance Signal |
title_fullStr |
Gold Nanoparticles Used as Protein Scavengers Enhance Surface Plasmon Resonance Signal |
title_full_unstemmed |
Gold Nanoparticles Used as Protein Scavengers Enhance Surface Plasmon Resonance Signal |
title_sort |
Gold Nanoparticles Used as Protein Scavengers Enhance Surface Plasmon Resonance Signal |
author |
de Macedo, Erenildo Ferreira [UNIFESP] |
author_facet |
de Macedo, Erenildo Ferreira [UNIFESP] Ducatti Formaggio, Daniela Maria [UNIFESP] Santos, Nivia Salles [UNIFESP] Tada, Dayane Batista [UNIFESP] |
author_role |
author |
author2 |
Ducatti Formaggio, Daniela Maria [UNIFESP] Santos, Nivia Salles [UNIFESP] Tada, Dayane Batista [UNIFESP] |
author2_role |
author author author |
dc.contributor.author.fl_str_mv |
de Macedo, Erenildo Ferreira [UNIFESP] Ducatti Formaggio, Daniela Maria [UNIFESP] Santos, Nivia Salles [UNIFESP] Tada, Dayane Batista [UNIFESP] |
dc.subject.eng.fl_str_mv |
surface plasmon resonance gold nanoparticles signal amplification lectins concanavalin A peanut agglutinin |
topic |
surface plasmon resonance gold nanoparticles signal amplification lectins concanavalin A peanut agglutinin |
description |
Although several researchers had reported on methodologies for surface plasmon resonance (SPR) signal amplification based on the use of nanoparticles (NPs), the majority addressed the sandwich technique and low protein concentration. In this work, a different approach for SPR signal enhancement based on the use of gold NPs was evaluated. The method was used in the detection of two lectins, peanut agglutinin (PNA) and concanavalin A (ConA). Gold NPs were functionalized with antibodies anti-PNA and anti-ConA, and these NPs were used as protein scavengers in a solution. After being incubated with solutions of PNA or ConA, the gold NPs coupled with the collected lectins were injected on the sensor containing the immobilized antibodies. The signal amplification provided by this method was compared to the signal amplification provided by the direct coupling of PNA and ConA to gold NPs. Furthermore, both methods, direct coupling and gold NPs as protein scavengers, were compared to the direct detection of PNA and ConA in solution. Compared to the analysis of free protein, the direct coupling of PNA and ConA to gold NPs resulted in a signal amplification of 10-40-fold and a 13-fold decrease of the limit of detection (LOD), whereas the use of gold NPs as protein scavengers resulted in an SPR signal 40-50-times higher and an LOD 64-times lower. |
publishDate |
2017 |
dc.date.issued.fl_str_mv |
2017 |
dc.date.accessioned.fl_str_mv |
2020-07-02T18:52:21Z |
dc.date.available.fl_str_mv |
2020-07-02T18:52:21Z |
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article |
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publishedVersion |
dc.identifier.citation.fl_str_mv |
Sensors. Basel, v. 17, n. 12, p. -, 2017. |
dc.identifier.uri.fl_str_mv |
https://repositorio.unifesp.br/handle/11600/54023 http://dx.doi.org/10.3390/s17122765 |
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1424-8220 |
dc.identifier.file.none.fl_str_mv |
WOS000423285800065.pdf |
dc.identifier.doi.none.fl_str_mv |
10.3390/s17122765 |
dc.identifier.wos.none.fl_str_mv |
WOS:000423285800065 |
identifier_str_mv |
Sensors. Basel, v. 17, n. 12, p. -, 2017. 1424-8220 WOS000423285800065.pdf 10.3390/s17122765 WOS:000423285800065 |
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https://repositorio.unifesp.br/handle/11600/54023 http://dx.doi.org/10.3390/s17122765 |
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