TRAP-silver staining, a highly sensitive assay for measuring telomerase activity in tumor tissue and cell lines
Autor(a) principal: | |
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Data de Publicação: | 2002 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNIFESP |
Texto Completo: | http://repositorio.unifesp.br/handle/11600/1315 http://dx.doi.org/10.1590/S0100-879X2002000100009 |
Resumo: | Measurement of telomerase activity in clinically obtained tumor samples may provide important information for use as both a diagnostic marker and a prognostic indicator for patient outcome. In order to evaluate telomerase activity in tumor tissue without radiolabeling the product, we developed a simple telomeric repeat amplification protocol-silver-staining assay that is less time-consuming, is safe and requires minimal equipment. In addition, we determined the sensitivity of the silver-staining method by using extracts of telomerase-positive thyroid carcinoma cell lines which were serially diluted from 5,000 to 10 cells. Telomerase activity was also assayed in 19 thyroid tumors, 2 normal controls and 27 bone marrow aspirates. The results indicate that the technique permits the detection of telomerase activity from 5000 to as few as 10 cells. We propose that it could be immediately applicable in many laboratories due to the minimal amount of equipment required. |
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Dalla Torre, Cristiane Arruda [UNIFESP]Maciel, Rui Monteiro de Barros [UNIFESP]Pinheiro, Nídia Alice [UNIFESP]Andrade, Joyce Anderson Duffles [UNIFESP]Toledo, Silvia Regina Caminada de [UNIFESP]Villa, Luisa LinaCerutti, Janete Maria [UNIFESP]Universidade Federal de São Paulo (UNIFESP)Instituto Ludwig de Pesquisa sobre o Câncer2015-06-14T13:29:34Z2015-06-14T13:29:34Z2002-01-01Brazilian Journal of Medical and Biological Research. Associação Brasileira de Divulgação Científica, v. 35, n. 1, p. 65-68, 2002.0100-879Xhttp://repositorio.unifesp.br/handle/11600/1315http://dx.doi.org/10.1590/S0100-879X2002000100009S0100-879X2002000100009.pdfS0100-879X200200010000910.1590/S0100-879X2002000100009WOS:000173942600009Measurement of telomerase activity in clinically obtained tumor samples may provide important information for use as both a diagnostic marker and a prognostic indicator for patient outcome. In order to evaluate telomerase activity in tumor tissue without radiolabeling the product, we developed a simple telomeric repeat amplification protocol-silver-staining assay that is less time-consuming, is safe and requires minimal equipment. In addition, we determined the sensitivity of the silver-staining method by using extracts of telomerase-positive thyroid carcinoma cell lines which were serially diluted from 5,000 to 10 cells. Telomerase activity was also assayed in 19 thyroid tumors, 2 normal controls and 27 bone marrow aspirates. The results indicate that the technique permits the detection of telomerase activity from 5000 to as few as 10 cells. We propose that it could be immediately applicable in many laboratories due to the minimal amount of equipment required.Universidade Federal de São Paulo (UNIFESP) Escola Paulista de Medicina Departamento de MedicinaUniversidade Federal de São Paulo (UNIFESP) Escola Paulista de Medicina Departamento de MorfologiaInstituto Ludwig de Pesquisa sobre o CâncerUNIFESP, EPM, Depto. de MedicinaUNIFESP, EPM, Depto. de MorfologiaSciELO65-68engAssociação Brasileira de Divulgação CientíficaBrazilian Journal of Medical and Biological ResearchTelomeric repeat amplification protocolTRAP assayHuman thyroid cancerLeukemiaTelomeraseSilver stainingTRAP-silver staining, a highly sensitive assay for measuring telomerase activity in tumor tissue and cell linesinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UNIFESPinstname:Universidade Federal de São Paulo (UNIFESP)instacron:UNIFESPORIGINALS0100-879X2002000100009.pdfapplication/pdf193437${dspace.ui.url}/bitstream/11600/1315/1/S0100-879X2002000100009.pdf5498ce0f1be3dcfad1dfdcfc76c26410MD51open accessTEXTS0100-879X2002000100009.pdf.txtS0100-879X2002000100009.pdf.txtExtracted texttext/plain16160${dspace.ui.url}/bitstream/11600/1315/2/S0100-879X2002000100009.pdf.txt0fc003067272acdf0db18726dca5c29cMD52open access11600/13152021-10-05 21:50:00.699open accessoai:repositorio.unifesp.br:11600/1315Repositório InstitucionalPUBhttp://www.repositorio.unifesp.br/oai/requestopendoar:34652021-10-06T00:50Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)false |
dc.title.en.fl_str_mv |
TRAP-silver staining, a highly sensitive assay for measuring telomerase activity in tumor tissue and cell lines |
title |
TRAP-silver staining, a highly sensitive assay for measuring telomerase activity in tumor tissue and cell lines |
spellingShingle |
TRAP-silver staining, a highly sensitive assay for measuring telomerase activity in tumor tissue and cell lines Dalla Torre, Cristiane Arruda [UNIFESP] Telomeric repeat amplification protocol TRAP assay Human thyroid cancer Leukemia Telomerase Silver staining |
title_short |
TRAP-silver staining, a highly sensitive assay for measuring telomerase activity in tumor tissue and cell lines |
title_full |
TRAP-silver staining, a highly sensitive assay for measuring telomerase activity in tumor tissue and cell lines |
title_fullStr |
TRAP-silver staining, a highly sensitive assay for measuring telomerase activity in tumor tissue and cell lines |
title_full_unstemmed |
TRAP-silver staining, a highly sensitive assay for measuring telomerase activity in tumor tissue and cell lines |
title_sort |
TRAP-silver staining, a highly sensitive assay for measuring telomerase activity in tumor tissue and cell lines |
author |
Dalla Torre, Cristiane Arruda [UNIFESP] |
author_facet |
Dalla Torre, Cristiane Arruda [UNIFESP] Maciel, Rui Monteiro de Barros [UNIFESP] Pinheiro, Nídia Alice [UNIFESP] Andrade, Joyce Anderson Duffles [UNIFESP] Toledo, Silvia Regina Caminada de [UNIFESP] Villa, Luisa Lina Cerutti, Janete Maria [UNIFESP] |
author_role |
author |
author2 |
Maciel, Rui Monteiro de Barros [UNIFESP] Pinheiro, Nídia Alice [UNIFESP] Andrade, Joyce Anderson Duffles [UNIFESP] Toledo, Silvia Regina Caminada de [UNIFESP] Villa, Luisa Lina Cerutti, Janete Maria [UNIFESP] |
author2_role |
author author author author author author |
dc.contributor.institution.none.fl_str_mv |
Universidade Federal de São Paulo (UNIFESP) Instituto Ludwig de Pesquisa sobre o Câncer |
dc.contributor.author.fl_str_mv |
Dalla Torre, Cristiane Arruda [UNIFESP] Maciel, Rui Monteiro de Barros [UNIFESP] Pinheiro, Nídia Alice [UNIFESP] Andrade, Joyce Anderson Duffles [UNIFESP] Toledo, Silvia Regina Caminada de [UNIFESP] Villa, Luisa Lina Cerutti, Janete Maria [UNIFESP] |
dc.subject.eng.fl_str_mv |
Telomeric repeat amplification protocol TRAP assay Human thyroid cancer Leukemia Telomerase Silver staining |
topic |
Telomeric repeat amplification protocol TRAP assay Human thyroid cancer Leukemia Telomerase Silver staining |
description |
Measurement of telomerase activity in clinically obtained tumor samples may provide important information for use as both a diagnostic marker and a prognostic indicator for patient outcome. In order to evaluate telomerase activity in tumor tissue without radiolabeling the product, we developed a simple telomeric repeat amplification protocol-silver-staining assay that is less time-consuming, is safe and requires minimal equipment. In addition, we determined the sensitivity of the silver-staining method by using extracts of telomerase-positive thyroid carcinoma cell lines which were serially diluted from 5,000 to 10 cells. Telomerase activity was also assayed in 19 thyroid tumors, 2 normal controls and 27 bone marrow aspirates. The results indicate that the technique permits the detection of telomerase activity from 5000 to as few as 10 cells. We propose that it could be immediately applicable in many laboratories due to the minimal amount of equipment required. |
publishDate |
2002 |
dc.date.issued.fl_str_mv |
2002-01-01 |
dc.date.accessioned.fl_str_mv |
2015-06-14T13:29:34Z |
dc.date.available.fl_str_mv |
2015-06-14T13:29:34Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
Brazilian Journal of Medical and Biological Research. Associação Brasileira de Divulgação Científica, v. 35, n. 1, p. 65-68, 2002. |
dc.identifier.uri.fl_str_mv |
http://repositorio.unifesp.br/handle/11600/1315 http://dx.doi.org/10.1590/S0100-879X2002000100009 |
dc.identifier.issn.none.fl_str_mv |
0100-879X |
dc.identifier.file.none.fl_str_mv |
S0100-879X2002000100009.pdf |
dc.identifier.scielo.none.fl_str_mv |
S0100-879X2002000100009 |
dc.identifier.doi.none.fl_str_mv |
10.1590/S0100-879X2002000100009 |
dc.identifier.wos.none.fl_str_mv |
WOS:000173942600009 |
identifier_str_mv |
Brazilian Journal of Medical and Biological Research. Associação Brasileira de Divulgação Científica, v. 35, n. 1, p. 65-68, 2002. 0100-879X S0100-879X2002000100009.pdf S0100-879X2002000100009 10.1590/S0100-879X2002000100009 WOS:000173942600009 |
url |
http://repositorio.unifesp.br/handle/11600/1315 http://dx.doi.org/10.1590/S0100-879X2002000100009 |
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eng |
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Brazilian Journal of Medical and Biological Research |
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openAccess |
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65-68 |
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Associação Brasileira de Divulgação Científica |
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Associação Brasileira de Divulgação Científica |
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