Antioxidant and Cytotoxic Effects of Crude Extract, Fractions and 4-Nerolidylcathecol from Aerial Parts of Pothomorphe umbellata L. (Piperaceae)
Autor(a) principal: | |
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Data de Publicação: | 2013 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNIFESP |
Texto Completo: | http://repositorio.unifesp.br/handle/11600/35683 http://dx.doi.org/10.1155/2013/206581 |
Resumo: | The crude ethanolic extract from aerial parts of Pothomorphe umbellata L. (Piperaceae) and fractions obtained by partitions sequentially among water-methanol, methylene chloride, and ethyl acetate, as well as the major constituent, 4-nerolidylcatechol, were, respectively, evaluated and evidenced for antioxidant and cytotoxic effects through fluorometric microplate and microculture tetrazolium assays in HL-60 cells. the crude ethanolic extract demonstrated the preeminent antioxidant activity (IC50 = 1.2 mu g/mL) against exogenous cytoplasmic reactive oxygen species, followed by the water-methanolic (IC50 = 4.5 mu g/mL), methylene chloride (IC50 = 5.9 mu g/mL), ethyl acetate (IC50 = 8.0 mu g/mL), 4-nerolidylcatechol (IC50 = 8.6 mu g/mL), and the sterol fractions (IC50 > 12.5 mu g/mL). Vitamin C, the positive control used in this assay, presented IC50 value equivalent to 1.7 mu g/mL. 4-Nerolidylcatechol (IC50 = 0.4 mu g/mL) and methylene chloride fraction (IC50 = 2.3 mu g/mL) presented considerable cytotoxicity probably because of the presence of an o-quinone, an auto-oxidation by product of the catechol. Polar compounds, present in the ethanol extract, appear to increase the solubility and stability of the major active constituent, acting synergistically with 4-nerolidylcatechol, improving its pharmacokinetic parameters and increasing significantly its antioxidant activity which, in turn, suggests that the aqueous-ethanolic extract, used in folklore medicine, is safe and effective. |
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Lopes, Andrey P. [UNIFESP]Bagatela, Bianca S. [UNIFESP]Rosa, Paulo C. P. [UNIFESP]Nanayakkara, Dhammika N. P.Tavares Carvalho, Jose CarlosMaistro, Edson L.Bastos, Jairo K.Perazzo, Fabio F. [UNIFESP]Universidade Federal de São Paulo (UNIFESP)Univ MississippiUniv Fed AmapaUniv Estadual PaulistaUniversidade de São Paulo (USP)2016-01-24T14:30:52Z2016-01-24T14:30:52Z2013-01-01Biomed Research International. New York: Hindawi Publishing Corporation, 5 p., 2013.2314-6133http://repositorio.unifesp.br/handle/11600/35683http://dx.doi.org/10.1155/2013/206581WOS000313686800001.pdf10.1155/2013/206581WOS:000313686800001The crude ethanolic extract from aerial parts of Pothomorphe umbellata L. (Piperaceae) and fractions obtained by partitions sequentially among water-methanol, methylene chloride, and ethyl acetate, as well as the major constituent, 4-nerolidylcatechol, were, respectively, evaluated and evidenced for antioxidant and cytotoxic effects through fluorometric microplate and microculture tetrazolium assays in HL-60 cells. the crude ethanolic extract demonstrated the preeminent antioxidant activity (IC50 = 1.2 mu g/mL) against exogenous cytoplasmic reactive oxygen species, followed by the water-methanolic (IC50 = 4.5 mu g/mL), methylene chloride (IC50 = 5.9 mu g/mL), ethyl acetate (IC50 = 8.0 mu g/mL), 4-nerolidylcatechol (IC50 = 8.6 mu g/mL), and the sterol fractions (IC50 > 12.5 mu g/mL). Vitamin C, the positive control used in this assay, presented IC50 value equivalent to 1.7 mu g/mL. 4-Nerolidylcatechol (IC50 = 0.4 mu g/mL) and methylene chloride fraction (IC50 = 2.3 mu g/mL) presented considerable cytotoxicity probably because of the presence of an o-quinone, an auto-oxidation by product of the catechol. Polar compounds, present in the ethanol extract, appear to increase the solubility and stability of the major active constituent, acting synergistically with 4-nerolidylcatechol, improving its pharmacokinetic parameters and increasing significantly its antioxidant activity which, in turn, suggests that the aqueous-ethanolic extract, used in folklore medicine, is safe and effective.Universidade Federal de São Paulo, Inst Ciencias Ambientais Quim & Farmaceut, Dept Ciencias Exatas & Terra, BR-09972270 Diadema, SP, BrazilUniv Mississippi, Pharmaceut Sci Res Inst, Natl Ctr Nat Prod Res, Oxford, MS 38677 USAUniv Fed Amapa, Lab Pesquisa Farmacos, BR-68902280 Macapa, AP, BrazilUniv Estadual Paulista, Fac Filosofia & Ciencias, Dept Fonoaudiol, BR-17525900 Marilia, SP, BrazilUniv São Paulo, Fac Ciencias Farmaceut Ribeirao Preto, Dept Ciencias Farmaceut, BR-14040903 Ribeirao Preto, SP, BrazilUniversidade Federal de São Paulo, Inst Ciencias Ambientais Quim & Farmaceut, Dept Ciencias Exatas & Terra, BR-09972270 Diadema, SP, BrazilWeb of Science5engHindawi Publishing CorporationBiomed Research InternationalAntioxidant and Cytotoxic Effects of Crude Extract, Fractions and 4-Nerolidylcathecol from Aerial Parts of Pothomorphe umbellata L. (Piperaceae)info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UNIFESPinstname:Universidade Federal de São Paulo (UNIFESP)instacron:UNIFESPORIGINALWOS000313686800001.pdfapplication/pdf1229065${dspace.ui.url}/bitstream/11600/35683/1/WOS000313686800001.pdf260f6cb50073af43dc9bb6e323cc6964MD51open accessTEXTWOS000313686800001.pdf.txtWOS000313686800001.pdf.txtExtracted texttext/plain26816${dspace.ui.url}/bitstream/11600/35683/2/WOS000313686800001.pdf.txt71583e9c6eeb3995da630d7854f1ae97MD52open access11600/356832023-02-15 11:39:38.423open accessoai:repositorio.unifesp.br:11600/35683Repositório InstitucionalPUBhttp://www.repositorio.unifesp.br/oai/requestopendoar:34652023-02-15T14:39:38Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)false |
dc.title.en.fl_str_mv |
Antioxidant and Cytotoxic Effects of Crude Extract, Fractions and 4-Nerolidylcathecol from Aerial Parts of Pothomorphe umbellata L. (Piperaceae) |
title |
Antioxidant and Cytotoxic Effects of Crude Extract, Fractions and 4-Nerolidylcathecol from Aerial Parts of Pothomorphe umbellata L. (Piperaceae) |
spellingShingle |
Antioxidant and Cytotoxic Effects of Crude Extract, Fractions and 4-Nerolidylcathecol from Aerial Parts of Pothomorphe umbellata L. (Piperaceae) Lopes, Andrey P. [UNIFESP] |
title_short |
Antioxidant and Cytotoxic Effects of Crude Extract, Fractions and 4-Nerolidylcathecol from Aerial Parts of Pothomorphe umbellata L. (Piperaceae) |
title_full |
Antioxidant and Cytotoxic Effects of Crude Extract, Fractions and 4-Nerolidylcathecol from Aerial Parts of Pothomorphe umbellata L. (Piperaceae) |
title_fullStr |
Antioxidant and Cytotoxic Effects of Crude Extract, Fractions and 4-Nerolidylcathecol from Aerial Parts of Pothomorphe umbellata L. (Piperaceae) |
title_full_unstemmed |
Antioxidant and Cytotoxic Effects of Crude Extract, Fractions and 4-Nerolidylcathecol from Aerial Parts of Pothomorphe umbellata L. (Piperaceae) |
title_sort |
Antioxidant and Cytotoxic Effects of Crude Extract, Fractions and 4-Nerolidylcathecol from Aerial Parts of Pothomorphe umbellata L. (Piperaceae) |
author |
Lopes, Andrey P. [UNIFESP] |
author_facet |
Lopes, Andrey P. [UNIFESP] Bagatela, Bianca S. [UNIFESP] Rosa, Paulo C. P. [UNIFESP] Nanayakkara, Dhammika N. P. Tavares Carvalho, Jose Carlos Maistro, Edson L. Bastos, Jairo K. Perazzo, Fabio F. [UNIFESP] |
author_role |
author |
author2 |
Bagatela, Bianca S. [UNIFESP] Rosa, Paulo C. P. [UNIFESP] Nanayakkara, Dhammika N. P. Tavares Carvalho, Jose Carlos Maistro, Edson L. Bastos, Jairo K. Perazzo, Fabio F. [UNIFESP] |
author2_role |
author author author author author author author |
dc.contributor.institution.none.fl_str_mv |
Universidade Federal de São Paulo (UNIFESP) Univ Mississippi Univ Fed Amapa Univ Estadual Paulista Universidade de São Paulo (USP) |
dc.contributor.author.fl_str_mv |
Lopes, Andrey P. [UNIFESP] Bagatela, Bianca S. [UNIFESP] Rosa, Paulo C. P. [UNIFESP] Nanayakkara, Dhammika N. P. Tavares Carvalho, Jose Carlos Maistro, Edson L. Bastos, Jairo K. Perazzo, Fabio F. [UNIFESP] |
description |
The crude ethanolic extract from aerial parts of Pothomorphe umbellata L. (Piperaceae) and fractions obtained by partitions sequentially among water-methanol, methylene chloride, and ethyl acetate, as well as the major constituent, 4-nerolidylcatechol, were, respectively, evaluated and evidenced for antioxidant and cytotoxic effects through fluorometric microplate and microculture tetrazolium assays in HL-60 cells. the crude ethanolic extract demonstrated the preeminent antioxidant activity (IC50 = 1.2 mu g/mL) against exogenous cytoplasmic reactive oxygen species, followed by the water-methanolic (IC50 = 4.5 mu g/mL), methylene chloride (IC50 = 5.9 mu g/mL), ethyl acetate (IC50 = 8.0 mu g/mL), 4-nerolidylcatechol (IC50 = 8.6 mu g/mL), and the sterol fractions (IC50 > 12.5 mu g/mL). Vitamin C, the positive control used in this assay, presented IC50 value equivalent to 1.7 mu g/mL. 4-Nerolidylcatechol (IC50 = 0.4 mu g/mL) and methylene chloride fraction (IC50 = 2.3 mu g/mL) presented considerable cytotoxicity probably because of the presence of an o-quinone, an auto-oxidation by product of the catechol. Polar compounds, present in the ethanol extract, appear to increase the solubility and stability of the major active constituent, acting synergistically with 4-nerolidylcatechol, improving its pharmacokinetic parameters and increasing significantly its antioxidant activity which, in turn, suggests that the aqueous-ethanolic extract, used in folklore medicine, is safe and effective. |
publishDate |
2013 |
dc.date.issued.fl_str_mv |
2013-01-01 |
dc.date.accessioned.fl_str_mv |
2016-01-24T14:30:52Z |
dc.date.available.fl_str_mv |
2016-01-24T14:30:52Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
Biomed Research International. New York: Hindawi Publishing Corporation, 5 p., 2013. |
dc.identifier.uri.fl_str_mv |
http://repositorio.unifesp.br/handle/11600/35683 http://dx.doi.org/10.1155/2013/206581 |
dc.identifier.issn.none.fl_str_mv |
2314-6133 |
dc.identifier.file.none.fl_str_mv |
WOS000313686800001.pdf |
dc.identifier.doi.none.fl_str_mv |
10.1155/2013/206581 |
dc.identifier.wos.none.fl_str_mv |
WOS:000313686800001 |
identifier_str_mv |
Biomed Research International. New York: Hindawi Publishing Corporation, 5 p., 2013. 2314-6133 WOS000313686800001.pdf 10.1155/2013/206581 WOS:000313686800001 |
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http://repositorio.unifesp.br/handle/11600/35683 http://dx.doi.org/10.1155/2013/206581 |
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eng |
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Hindawi Publishing Corporation |
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