Human autoantibodies to diacyl-phosphatidylethanolamine recognize a specific set of discrete cytoplasmic domains
Autor(a) principal: | |
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Data de Publicação: | 2006 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNIFESP |
Texto Completo: | http://repositorio.unifesp.br/handle/11600/28755 http://dx.doi.org/10.1111/j.1365-2249.2006.03024.x |
Resumo: | The aim of this study was to characterize a novel human autoantibody-autoantigen system represented as cytoplasmic discrete speckles (CDS) in indirect immunofluorescence (IIF). A distinct CDS IIF pattern represented by 3-20 discrete speckles dispersed throughout the cytoplasm was identified among other cytoplasmic speckled IIF patterns. the cytoplasmic domains labelled by human anti-CDS-1 antibodies did not co-localize with endosome/lysosome markers EEA1 and LAMP-2, but showed partial co-localization with glycine-tryptophan bodies (GWB). CDS-1 sera did not react with several cellular extracts in immunoblotting and did not immunoprecipitate recombinant GW182 or EEA1 proteins. the typical CDS-1 IIF labelling pattern was abolished after delipidation of HEp-2 cells. Moreover, CDS-1 sera reacted strongly with a lipid component co-migrating with phosphatidylethanolamine (PE) in high performance thin-layer chromatography (HPTLC)-immunostaining of HEp-2 cell total lipid extracts. the CDS-1 major molecular targets were established by electrospray ionization-mass spectrometry (ESI-MS), HPTLC-immunostaining and chemiluminescent enzyme-linked immunosorbent assay as diacyl-PE species, containing preferentially a cis-C18 : 1 fatty acid chain at C-2 of the glycerol moiety, namely 1,2-cis-C18 : 1-PE and 1-C16 : 0-2-cis-C18 : 1-PE. the clinical association of CDS-1 sera included a variety of systemic and organ-specific autoimmune diseases but they were also observed in patients with no evidence of autoimmune disease. |
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Laurino, CCFCFritzler, M. J.Mortara, Renato Arruda [UNIFESP]Silva, N. P.Almeida, I. C.Andrade, LECUniversidade Federal de São Paulo (UNIFESP)Univ CalgaryUniv TexasUniversidade de São Paulo (USP)2016-01-24T12:40:59Z2016-01-24T12:40:59Z2006-03-01Clinical and Experimental Immunology. Oxford: Blackwell Publishing, v. 143, n. 3, p. 572-584, 2006.0009-9104http://repositorio.unifesp.br/handle/11600/28755http://dx.doi.org/10.1111/j.1365-2249.2006.03024.x10.1111/j.1365-2249.2006.03024.xWOS:000235370200022The aim of this study was to characterize a novel human autoantibody-autoantigen system represented as cytoplasmic discrete speckles (CDS) in indirect immunofluorescence (IIF). A distinct CDS IIF pattern represented by 3-20 discrete speckles dispersed throughout the cytoplasm was identified among other cytoplasmic speckled IIF patterns. the cytoplasmic domains labelled by human anti-CDS-1 antibodies did not co-localize with endosome/lysosome markers EEA1 and LAMP-2, but showed partial co-localization with glycine-tryptophan bodies (GWB). CDS-1 sera did not react with several cellular extracts in immunoblotting and did not immunoprecipitate recombinant GW182 or EEA1 proteins. the typical CDS-1 IIF labelling pattern was abolished after delipidation of HEp-2 cells. Moreover, CDS-1 sera reacted strongly with a lipid component co-migrating with phosphatidylethanolamine (PE) in high performance thin-layer chromatography (HPTLC)-immunostaining of HEp-2 cell total lipid extracts. the CDS-1 major molecular targets were established by electrospray ionization-mass spectrometry (ESI-MS), HPTLC-immunostaining and chemiluminescent enzyme-linked immunosorbent assay as diacyl-PE species, containing preferentially a cis-C18 : 1 fatty acid chain at C-2 of the glycerol moiety, namely 1,2-cis-C18 : 1-PE and 1-C16 : 0-2-cis-C18 : 1-PE. the clinical association of CDS-1 sera included a variety of systemic and organ-specific autoimmune diseases but they were also observed in patients with no evidence of autoimmune disease.Universidade Federal de São Paulo, Div Rheumatol, Escola Paulista Med, BR-04023062 São Paulo, BrazilUniv Calgary, Dept Biochem & Mol Biol, Calgary, AB, CanadaUNIFESP, EPM, Dept Microbiol Imunol & Parasitol, São Paulo, BrazilUniv Texas, Dept Biol Sci, El Paso, TX 79968 USAUniv São Paulo, ICB, Dept Parasitol, São Paulo, BrazilUniversidade Federal de São Paulo, Div Rheumatol, Escola Paulista Med, BR-04023062 São Paulo, BrazilUNIFESP, EPM, Dept Microbiol Imunol & Parasitol, São Paulo, BrazilWeb of Science572-584engBlackwell PublishingClinical and Experimental Immunologyautoantibodiesautoantigenepitope analysiscytoplasmic domainsphospholipidsGW bodiesHuman autoantibodies to diacyl-phosphatidylethanolamine recognize a specific set of discrete cytoplasmic domainsinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UNIFESPinstname:Universidade Federal de São Paulo (UNIFESP)instacron:UNIFESP11600/287552022-02-08 11:52:09.978metadata only accessoai:repositorio.unifesp.br:11600/28755Repositório InstitucionalPUBhttp://www.repositorio.unifesp.br/oai/requestopendoar:34652022-02-08T14:52:09Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)false |
dc.title.en.fl_str_mv |
Human autoantibodies to diacyl-phosphatidylethanolamine recognize a specific set of discrete cytoplasmic domains |
title |
Human autoantibodies to diacyl-phosphatidylethanolamine recognize a specific set of discrete cytoplasmic domains |
spellingShingle |
Human autoantibodies to diacyl-phosphatidylethanolamine recognize a specific set of discrete cytoplasmic domains Laurino, CCFC autoantibodies autoantigen epitope analysis cytoplasmic domains phospholipids GW bodies |
title_short |
Human autoantibodies to diacyl-phosphatidylethanolamine recognize a specific set of discrete cytoplasmic domains |
title_full |
Human autoantibodies to diacyl-phosphatidylethanolamine recognize a specific set of discrete cytoplasmic domains |
title_fullStr |
Human autoantibodies to diacyl-phosphatidylethanolamine recognize a specific set of discrete cytoplasmic domains |
title_full_unstemmed |
Human autoantibodies to diacyl-phosphatidylethanolamine recognize a specific set of discrete cytoplasmic domains |
title_sort |
Human autoantibodies to diacyl-phosphatidylethanolamine recognize a specific set of discrete cytoplasmic domains |
author |
Laurino, CCFC |
author_facet |
Laurino, CCFC Fritzler, M. J. Mortara, Renato Arruda [UNIFESP] Silva, N. P. Almeida, I. C. Andrade, LEC |
author_role |
author |
author2 |
Fritzler, M. J. Mortara, Renato Arruda [UNIFESP] Silva, N. P. Almeida, I. C. Andrade, LEC |
author2_role |
author author author author author |
dc.contributor.institution.none.fl_str_mv |
Universidade Federal de São Paulo (UNIFESP) Univ Calgary Univ Texas Universidade de São Paulo (USP) |
dc.contributor.author.fl_str_mv |
Laurino, CCFC Fritzler, M. J. Mortara, Renato Arruda [UNIFESP] Silva, N. P. Almeida, I. C. Andrade, LEC |
dc.subject.eng.fl_str_mv |
autoantibodies autoantigen epitope analysis cytoplasmic domains phospholipids GW bodies |
topic |
autoantibodies autoantigen epitope analysis cytoplasmic domains phospholipids GW bodies |
description |
The aim of this study was to characterize a novel human autoantibody-autoantigen system represented as cytoplasmic discrete speckles (CDS) in indirect immunofluorescence (IIF). A distinct CDS IIF pattern represented by 3-20 discrete speckles dispersed throughout the cytoplasm was identified among other cytoplasmic speckled IIF patterns. the cytoplasmic domains labelled by human anti-CDS-1 antibodies did not co-localize with endosome/lysosome markers EEA1 and LAMP-2, but showed partial co-localization with glycine-tryptophan bodies (GWB). CDS-1 sera did not react with several cellular extracts in immunoblotting and did not immunoprecipitate recombinant GW182 or EEA1 proteins. the typical CDS-1 IIF labelling pattern was abolished after delipidation of HEp-2 cells. Moreover, CDS-1 sera reacted strongly with a lipid component co-migrating with phosphatidylethanolamine (PE) in high performance thin-layer chromatography (HPTLC)-immunostaining of HEp-2 cell total lipid extracts. the CDS-1 major molecular targets were established by electrospray ionization-mass spectrometry (ESI-MS), HPTLC-immunostaining and chemiluminescent enzyme-linked immunosorbent assay as diacyl-PE species, containing preferentially a cis-C18 : 1 fatty acid chain at C-2 of the glycerol moiety, namely 1,2-cis-C18 : 1-PE and 1-C16 : 0-2-cis-C18 : 1-PE. the clinical association of CDS-1 sera included a variety of systemic and organ-specific autoimmune diseases but they were also observed in patients with no evidence of autoimmune disease. |
publishDate |
2006 |
dc.date.issued.fl_str_mv |
2006-03-01 |
dc.date.accessioned.fl_str_mv |
2016-01-24T12:40:59Z |
dc.date.available.fl_str_mv |
2016-01-24T12:40:59Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
Clinical and Experimental Immunology. Oxford: Blackwell Publishing, v. 143, n. 3, p. 572-584, 2006. |
dc.identifier.uri.fl_str_mv |
http://repositorio.unifesp.br/handle/11600/28755 http://dx.doi.org/10.1111/j.1365-2249.2006.03024.x |
dc.identifier.issn.none.fl_str_mv |
0009-9104 |
dc.identifier.doi.none.fl_str_mv |
10.1111/j.1365-2249.2006.03024.x |
dc.identifier.wos.none.fl_str_mv |
WOS:000235370200022 |
identifier_str_mv |
Clinical and Experimental Immunology. Oxford: Blackwell Publishing, v. 143, n. 3, p. 572-584, 2006. 0009-9104 10.1111/j.1365-2249.2006.03024.x WOS:000235370200022 |
url |
http://repositorio.unifesp.br/handle/11600/28755 http://dx.doi.org/10.1111/j.1365-2249.2006.03024.x |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.ispartof.none.fl_str_mv |
Clinical and Experimental Immunology |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
572-584 |
dc.publisher.none.fl_str_mv |
Blackwell Publishing |
publisher.none.fl_str_mv |
Blackwell Publishing |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da UNIFESP instname:Universidade Federal de São Paulo (UNIFESP) instacron:UNIFESP |
instname_str |
Universidade Federal de São Paulo (UNIFESP) |
instacron_str |
UNIFESP |
institution |
UNIFESP |
reponame_str |
Repositório Institucional da UNIFESP |
collection |
Repositório Institucional da UNIFESP |
repository.name.fl_str_mv |
Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP) |
repository.mail.fl_str_mv |
|
_version_ |
1802764114516246528 |