Chromomycin A2 Induces Autophagy in Melanoma Cells
Autor(a) principal: | |
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Data de Publicação: | 2014 |
Outros Autores: | , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNIFESP |
Texto Completo: | http://repositorio.unifesp.br/handle/11600/38488 http://dx.doi.org/10.3390/md12125839 |
Resumo: | The present study highlights the biological effects of chromomycin A2 toward metastatic melanoma cells in culture. Besides chromomycin A2, chromomycin A3 and demethylchromomycin A2 were also identified from the extract derived from Streptomyces sp., recovered from Paracuru Beach, located in the northeast region of Brazil. the cytotoxic activity of chromomycin A2 was evaluated across a panel of human tumor cell lines, which found IC50 values in the nM-range for exposures of 48 and 72 h. MALME-3M, a metastatic melanoma cell line, showed the highest sensitivity to chromomycin A2 after 48h incubation, and was chosen as a model to investigate this potent cytotoxic effect. Treatment with chromomycin A2 at 30 nM reduced cell proliferation, but had no significant effect upon cell viability. Additionally, chromomycin A2 induced accumulation of cells in G(0)/G(1) phase of the cell cycle, with consequent reduction of S and G(2)/M and unbalanced expression of cyclins. Chromomycin A2 treated cells depicted several cellular fragments resembling autophagosomes and increased expression of proteins LC3-A and LC3-B. Moreover, exposure to chromomycin A2 also induced the appearance of acidic vacuolar organelles in treated cells. These features combined are suggestive of the induction of autophagy promoted by chromomycin A2, a feature not previously described for chromomycins. |
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Guimaraes, Larissa AlvesJimenez, Paula Christine [UNIFESP]Silva Sousa, Thiciana daFreitas, Hozana Patricia S.Rocha, Danilo DamascenoWilke, Diego VerasMartin, JesusReyes, FernandoLoiola Pessoa, Otilia DeusdeniaCosta-Lotufo, Leticia VerasUniv Fed CearaUniversidade Federal de São Paulo (UNIFESP)Fdn MEDINA2016-01-24T14:38:13Z2016-01-24T14:38:13Z2014-12-01Marine Drugs. Basel: Mdpi Ag, v. 12, n. 12, p. 5839-5855, 2014.1660-3397http://repositorio.unifesp.br/handle/11600/38488http://dx.doi.org/10.3390/md12125839WOS000346796500006.pdf10.3390/md12125839WOS:000346796500006The present study highlights the biological effects of chromomycin A2 toward metastatic melanoma cells in culture. Besides chromomycin A2, chromomycin A3 and demethylchromomycin A2 were also identified from the extract derived from Streptomyces sp., recovered from Paracuru Beach, located in the northeast region of Brazil. the cytotoxic activity of chromomycin A2 was evaluated across a panel of human tumor cell lines, which found IC50 values in the nM-range for exposures of 48 and 72 h. MALME-3M, a metastatic melanoma cell line, showed the highest sensitivity to chromomycin A2 after 48h incubation, and was chosen as a model to investigate this potent cytotoxic effect. Treatment with chromomycin A2 at 30 nM reduced cell proliferation, but had no significant effect upon cell viability. Additionally, chromomycin A2 induced accumulation of cells in G(0)/G(1) phase of the cell cycle, with consequent reduction of S and G(2)/M and unbalanced expression of cyclins. Chromomycin A2 treated cells depicted several cellular fragments resembling autophagosomes and increased expression of proteins LC3-A and LC3-B. Moreover, exposure to chromomycin A2 also induced the appearance of acidic vacuolar organelles in treated cells. These features combined are suggestive of the induction of autophagy promoted by chromomycin A2, a feature not previously described for chromomycins.Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Fundacao Cearense de Apoio ao Desenvolvimento Cientifico e Tecnologico (FUNCAP-Programa Areas Estrategicas)Univ Fed Ceara, Inst Marine Sci, BR-60165081 Fortaleza, Ceara, BrazilUniv Fed Ceara, Dept Physiol & Pharmacol, BR-60430270 Fortaleza, Ceara, BrazilUniversidade Federal de São Paulo, Dept Marine Sci, BR-11030400 São Paulo, BrazilUniv Fed Ceara, Dept Organ & Inorgan Chem, BR-60021970 Fortaleza, Ceara, BrazilFdn MEDINA, Ctr Excelencia Invest Medicamentos Innovadores An, Granada 18016, SpainUniversidade Federal de São Paulo, Dept Marine Sci, BR-11030400 São Paulo, BrazilWeb of Science5839-5855engMdpi AgMarine DrugsStreptomycesautophagycytotoxicitychromomycinmarine microorganismsBrazilChromomycin A2 Induces Autophagy in Melanoma Cellsinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UNIFESPinstname:Universidade Federal de São Paulo (UNIFESP)instacron:UNIFESPORIGINALWOS000346796500006.pdfapplication/pdf1066478${dspace.ui.url}/bitstream/11600/38488/1/WOS000346796500006.pdf4427ad1229ddfac2aa4884fae9b2721cMD51open accessTEXTWOS000346796500006.pdf.txtWOS000346796500006.pdf.txtExtracted texttext/plain51670${dspace.ui.url}/bitstream/11600/38488/6/WOS000346796500006.pdf.txta8ce11255d420beb7efb9f4c6df8a697MD56open accessTHUMBNAILWOS000346796500006.pdf.jpgWOS000346796500006.pdf.jpgIM Thumbnailimage/jpeg6957${dspace.ui.url}/bitstream/11600/38488/8/WOS000346796500006.pdf.jpgb7c8bbff9e231d2dc935e99522022d11MD58open access11600/384882023-06-05 19:38:55.616open accessoai:repositorio.unifesp.br:11600/38488Repositório InstitucionalPUBhttp://www.repositorio.unifesp.br/oai/requestopendoar:34652023-06-05T22:38:55Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)false |
dc.title.en.fl_str_mv |
Chromomycin A2 Induces Autophagy in Melanoma Cells |
title |
Chromomycin A2 Induces Autophagy in Melanoma Cells |
spellingShingle |
Chromomycin A2 Induces Autophagy in Melanoma Cells Guimaraes, Larissa Alves Streptomyces autophagy cytotoxicity chromomycin marine microorganisms Brazil |
title_short |
Chromomycin A2 Induces Autophagy in Melanoma Cells |
title_full |
Chromomycin A2 Induces Autophagy in Melanoma Cells |
title_fullStr |
Chromomycin A2 Induces Autophagy in Melanoma Cells |
title_full_unstemmed |
Chromomycin A2 Induces Autophagy in Melanoma Cells |
title_sort |
Chromomycin A2 Induces Autophagy in Melanoma Cells |
author |
Guimaraes, Larissa Alves |
author_facet |
Guimaraes, Larissa Alves Jimenez, Paula Christine [UNIFESP] Silva Sousa, Thiciana da Freitas, Hozana Patricia S. Rocha, Danilo Damasceno Wilke, Diego Veras Martin, Jesus Reyes, Fernando Loiola Pessoa, Otilia Deusdenia Costa-Lotufo, Leticia Veras |
author_role |
author |
author2 |
Jimenez, Paula Christine [UNIFESP] Silva Sousa, Thiciana da Freitas, Hozana Patricia S. Rocha, Danilo Damasceno Wilke, Diego Veras Martin, Jesus Reyes, Fernando Loiola Pessoa, Otilia Deusdenia Costa-Lotufo, Leticia Veras |
author2_role |
author author author author author author author author author |
dc.contributor.institution.none.fl_str_mv |
Univ Fed Ceara Universidade Federal de São Paulo (UNIFESP) Fdn MEDINA |
dc.contributor.author.fl_str_mv |
Guimaraes, Larissa Alves Jimenez, Paula Christine [UNIFESP] Silva Sousa, Thiciana da Freitas, Hozana Patricia S. Rocha, Danilo Damasceno Wilke, Diego Veras Martin, Jesus Reyes, Fernando Loiola Pessoa, Otilia Deusdenia Costa-Lotufo, Leticia Veras |
dc.subject.eng.fl_str_mv |
Streptomyces autophagy cytotoxicity chromomycin marine microorganisms Brazil |
topic |
Streptomyces autophagy cytotoxicity chromomycin marine microorganisms Brazil |
description |
The present study highlights the biological effects of chromomycin A2 toward metastatic melanoma cells in culture. Besides chromomycin A2, chromomycin A3 and demethylchromomycin A2 were also identified from the extract derived from Streptomyces sp., recovered from Paracuru Beach, located in the northeast region of Brazil. the cytotoxic activity of chromomycin A2 was evaluated across a panel of human tumor cell lines, which found IC50 values in the nM-range for exposures of 48 and 72 h. MALME-3M, a metastatic melanoma cell line, showed the highest sensitivity to chromomycin A2 after 48h incubation, and was chosen as a model to investigate this potent cytotoxic effect. Treatment with chromomycin A2 at 30 nM reduced cell proliferation, but had no significant effect upon cell viability. Additionally, chromomycin A2 induced accumulation of cells in G(0)/G(1) phase of the cell cycle, with consequent reduction of S and G(2)/M and unbalanced expression of cyclins. Chromomycin A2 treated cells depicted several cellular fragments resembling autophagosomes and increased expression of proteins LC3-A and LC3-B. Moreover, exposure to chromomycin A2 also induced the appearance of acidic vacuolar organelles in treated cells. These features combined are suggestive of the induction of autophagy promoted by chromomycin A2, a feature not previously described for chromomycins. |
publishDate |
2014 |
dc.date.issued.fl_str_mv |
2014-12-01 |
dc.date.accessioned.fl_str_mv |
2016-01-24T14:38:13Z |
dc.date.available.fl_str_mv |
2016-01-24T14:38:13Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
Marine Drugs. Basel: Mdpi Ag, v. 12, n. 12, p. 5839-5855, 2014. |
dc.identifier.uri.fl_str_mv |
http://repositorio.unifesp.br/handle/11600/38488 http://dx.doi.org/10.3390/md12125839 |
dc.identifier.issn.none.fl_str_mv |
1660-3397 |
dc.identifier.file.none.fl_str_mv |
WOS000346796500006.pdf |
dc.identifier.doi.none.fl_str_mv |
10.3390/md12125839 |
dc.identifier.wos.none.fl_str_mv |
WOS:000346796500006 |
identifier_str_mv |
Marine Drugs. Basel: Mdpi Ag, v. 12, n. 12, p. 5839-5855, 2014. 1660-3397 WOS000346796500006.pdf 10.3390/md12125839 WOS:000346796500006 |
url |
http://repositorio.unifesp.br/handle/11600/38488 http://dx.doi.org/10.3390/md12125839 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.ispartof.none.fl_str_mv |
Marine Drugs |
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info:eu-repo/semantics/openAccess |
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openAccess |
dc.format.none.fl_str_mv |
5839-5855 |
dc.publisher.none.fl_str_mv |
Mdpi Ag |
publisher.none.fl_str_mv |
Mdpi Ag |
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reponame:Repositório Institucional da UNIFESP instname:Universidade Federal de São Paulo (UNIFESP) instacron:UNIFESP |
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Universidade Federal de São Paulo (UNIFESP) |
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Repositório Institucional da UNIFESP |
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