The Bacterium Endosymbiont of Crithidia deanei Undergoes Coordinated Division with the Host Cell Nucleus
Autor(a) principal: | |
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Data de Publicação: | 2010 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNIFESP |
Texto Completo: | http://repositorio.unifesp.br/handle/11600/32826 http://dx.doi.org/10.1371/journal.pone.0012415 |
Resumo: | In trypanosomatids, cell division involves morphological changes and requires coordinated replication and segregation of the nucleus, kinetoplast and flagellum. in endosymbiont-containing trypanosomatids, like Crithidia deanei, this process is more complex, as each daughter cell contains only a single symbiotic bacterium, indicating that the prokaryote must replicate synchronically with the host protozoan. in this study, we used light and electron microscopy combined with three-dimensional reconstruction approaches to observe the endosymbiont shape and division during C. deanei cell cycle. We found that the bacterium replicates before the basal body and kinetoplast segregations and that the nucleus is the last organelle to divide, before cytokinesis. in addition, the endosymbiont is usually found close to the host cell nucleus, presenting different shapes during the protozoan cell cycle. Considering that the endosymbiosis in trypanosomatids is a mutualistic relationship, which resembles organelle acquisition during evolution, these findings establish an excellent model for the understanding of mechanisms related with the establishment of organelles in eukaryotic cells. |
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Machado Motta, Maria CristinaCosta Catta-Preta, Carolina MouraSchenkman, Sergio [UNIFESP]Azevedo Martins, Allan Cezar deMiranda, KildareSouza, Wanderley deElias, Maria CarolinaUniversidade Federal do Rio de Janeiro (UFRJ)Inst Nacl Ciencia & Tecnol Biol Estrut & BioimageUniversidade Federal de São Paulo (UNIFESP)Inst Nacl Metrol Normalizacao & Qualidade Ind InInst Butantan2016-01-24T14:05:20Z2016-01-24T14:05:20Z2010-08-26Plos One. San Francisco: Public Library Science, v. 5, n. 8, 9 p., 2010.1932-6203http://repositorio.unifesp.br/handle/11600/32826http://dx.doi.org/10.1371/journal.pone.0012415WOS000281292100015.pdf10.1371/journal.pone.0012415WOS:000281292100015In trypanosomatids, cell division involves morphological changes and requires coordinated replication and segregation of the nucleus, kinetoplast and flagellum. in endosymbiont-containing trypanosomatids, like Crithidia deanei, this process is more complex, as each daughter cell contains only a single symbiotic bacterium, indicating that the prokaryote must replicate synchronically with the host protozoan. in this study, we used light and electron microscopy combined with three-dimensional reconstruction approaches to observe the endosymbiont shape and division during C. deanei cell cycle. We found that the bacterium replicates before the basal body and kinetoplast segregations and that the nucleus is the last organelle to divide, before cytokinesis. in addition, the endosymbiont is usually found close to the host cell nucleus, presenting different shapes during the protozoan cell cycle. Considering that the endosymbiosis in trypanosomatids is a mutualistic relationship, which resembles organelle acquisition during evolution, these findings establish an excellent model for the understanding of mechanisms related with the establishment of organelles in eukaryotic cells.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Fundação de Amparo à Pesquisa do Estado do Rio de Janeiro (FAPERJ)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Univ Fed Rio de Janeiro, Inst Biofis Carlos Chagas Filho, Lab Ultraestrutura Celular Hertha Meyer, BR-21941 Rio de Janeiro, BrazilInst Nacl Ciencia & Tecnol Biol Estrut & Bioimage, Rio de Janeiro, BrazilUniversidade Federal de São Paulo, Dept Microbiol Imunol & Parasitol, São Paulo, BrazilInst Nacl Metrol Normalizacao & Qualidade Ind In, Rio de Janeiro, BrazilInst Butantan, Parasitol Lab, São Paulo, BrazilUniversidade Federal de São Paulo, Dept Microbiol Imunol & Parasitol, São Paulo, BrazilWeb of Science9engPublic Library SciencePlos OneThe Bacterium Endosymbiont of Crithidia deanei Undergoes Coordinated Division with the Host Cell Nucleusinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UNIFESPinstname:Universidade Federal de São Paulo (UNIFESP)instacron:UNIFESPORIGINALWOS000281292100015.pdfapplication/pdf1630664${dspace.ui.url}/bitstream/11600/32826/1/WOS000281292100015.pdf708f440e4814e4439571b813682b2b96MD51open accessTEXTWOS000281292100015.pdf.txtWOS000281292100015.pdf.txtExtracted texttext/plain43364${dspace.ui.url}/bitstream/11600/32826/2/WOS000281292100015.pdf.txtc67c0b1fa9f0b1d1b63f91436bc232c8MD52open access11600/328262023-01-12 22:12:03.454open accessoai:repositorio.unifesp.br:11600/32826Repositório InstitucionalPUBhttp://www.repositorio.unifesp.br/oai/requestopendoar:34652023-01-13T01:12:03Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)false |
dc.title.en.fl_str_mv |
The Bacterium Endosymbiont of Crithidia deanei Undergoes Coordinated Division with the Host Cell Nucleus |
title |
The Bacterium Endosymbiont of Crithidia deanei Undergoes Coordinated Division with the Host Cell Nucleus |
spellingShingle |
The Bacterium Endosymbiont of Crithidia deanei Undergoes Coordinated Division with the Host Cell Nucleus Machado Motta, Maria Cristina |
title_short |
The Bacterium Endosymbiont of Crithidia deanei Undergoes Coordinated Division with the Host Cell Nucleus |
title_full |
The Bacterium Endosymbiont of Crithidia deanei Undergoes Coordinated Division with the Host Cell Nucleus |
title_fullStr |
The Bacterium Endosymbiont of Crithidia deanei Undergoes Coordinated Division with the Host Cell Nucleus |
title_full_unstemmed |
The Bacterium Endosymbiont of Crithidia deanei Undergoes Coordinated Division with the Host Cell Nucleus |
title_sort |
The Bacterium Endosymbiont of Crithidia deanei Undergoes Coordinated Division with the Host Cell Nucleus |
author |
Machado Motta, Maria Cristina |
author_facet |
Machado Motta, Maria Cristina Costa Catta-Preta, Carolina Moura Schenkman, Sergio [UNIFESP] Azevedo Martins, Allan Cezar de Miranda, Kildare Souza, Wanderley de Elias, Maria Carolina |
author_role |
author |
author2 |
Costa Catta-Preta, Carolina Moura Schenkman, Sergio [UNIFESP] Azevedo Martins, Allan Cezar de Miranda, Kildare Souza, Wanderley de Elias, Maria Carolina |
author2_role |
author author author author author author |
dc.contributor.institution.none.fl_str_mv |
Universidade Federal do Rio de Janeiro (UFRJ) Inst Nacl Ciencia & Tecnol Biol Estrut & Bioimage Universidade Federal de São Paulo (UNIFESP) Inst Nacl Metrol Normalizacao & Qualidade Ind In Inst Butantan |
dc.contributor.author.fl_str_mv |
Machado Motta, Maria Cristina Costa Catta-Preta, Carolina Moura Schenkman, Sergio [UNIFESP] Azevedo Martins, Allan Cezar de Miranda, Kildare Souza, Wanderley de Elias, Maria Carolina |
description |
In trypanosomatids, cell division involves morphological changes and requires coordinated replication and segregation of the nucleus, kinetoplast and flagellum. in endosymbiont-containing trypanosomatids, like Crithidia deanei, this process is more complex, as each daughter cell contains only a single symbiotic bacterium, indicating that the prokaryote must replicate synchronically with the host protozoan. in this study, we used light and electron microscopy combined with three-dimensional reconstruction approaches to observe the endosymbiont shape and division during C. deanei cell cycle. We found that the bacterium replicates before the basal body and kinetoplast segregations and that the nucleus is the last organelle to divide, before cytokinesis. in addition, the endosymbiont is usually found close to the host cell nucleus, presenting different shapes during the protozoan cell cycle. Considering that the endosymbiosis in trypanosomatids is a mutualistic relationship, which resembles organelle acquisition during evolution, these findings establish an excellent model for the understanding of mechanisms related with the establishment of organelles in eukaryotic cells. |
publishDate |
2010 |
dc.date.issued.fl_str_mv |
2010-08-26 |
dc.date.accessioned.fl_str_mv |
2016-01-24T14:05:20Z |
dc.date.available.fl_str_mv |
2016-01-24T14:05:20Z |
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info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
Plos One. San Francisco: Public Library Science, v. 5, n. 8, 9 p., 2010. |
dc.identifier.uri.fl_str_mv |
http://repositorio.unifesp.br/handle/11600/32826 http://dx.doi.org/10.1371/journal.pone.0012415 |
dc.identifier.issn.none.fl_str_mv |
1932-6203 |
dc.identifier.file.none.fl_str_mv |
WOS000281292100015.pdf |
dc.identifier.doi.none.fl_str_mv |
10.1371/journal.pone.0012415 |
dc.identifier.wos.none.fl_str_mv |
WOS:000281292100015 |
identifier_str_mv |
Plos One. San Francisco: Public Library Science, v. 5, n. 8, 9 p., 2010. 1932-6203 WOS000281292100015.pdf 10.1371/journal.pone.0012415 WOS:000281292100015 |
url |
http://repositorio.unifesp.br/handle/11600/32826 http://dx.doi.org/10.1371/journal.pone.0012415 |
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eng |
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Public Library Science |
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Public Library Science |
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