Mesenchymal stem cells from sternum: the type of heart disease, ischemic or valvular, does not influence the cell culture establishment and growth kinetics

• Autor(a) principal: Dias, Lucinara Dadda
• Data de Publicação: 2017
• Outros Autores: Casali, Karina Rabello [UNIFESP], Ghem, Carine, da Silva, Melissa Kristocheck, Sausen, Grasiele, Palma, Patricia Bonini, Covas, Dimas Tadeu, Kalil, Renato A. K., Schaan, Beatriz D., Nardi, Nance Beyer, Markoski, Melissa Medeiros
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Repositório Institucional da UNIFESP
• Texto Completo: http://dx.doi.org/10.1186/s12967-017-1262-0; https://repositorio.unifesp.br/handle/11600/53434
• topic: MSC establishment; Stem cells culture; Cell therapy; Valvular heart disease; Ischemic heart disease

Background: In an attempt to increase the therapeutic potential for myocardial regeneration, there is a quest for new cell sources and types for cell therapy protocols. The pathophysiology of heart diseases may affect cellular characteristics and therapeutic results. Methods: To study the proliferative and differentiation potential of mesenchymal stem cells (MSC), isolated from bone marrow (BM) of sternum, we made a comparative analysis between samples of patients with ischemic (IHD) or non-ischemic valvular (VHD) heart diseases. We included patients with IHD (n = 42) or VHD (n = 20), with average age of 60 years and no differences in cardiovascular risk factors. BM samples were collected (16.4 +/- 6 mL) and submitted to centrifugation with Ficoll-Paque, yielding 4.5 +/- 1.5 x 10(7) cells/mL. Results: Morphology, immunophenotype and differentiation ability had proven that the cultivated sternal BM cells had MSC features. The colony forming unit-fibroblast (CFU-F) frequency was similar between groups (p = 0.510), but VHD samples showed positive correlation to plated cells vs. CFU-F number (r = 0.499, p = 0.049). The MSC culture was established in 29% of collected samples, achieved passage 9, without significant difference in expansion kinetics between groups (p > 0.05). Dyslipidemia and the use of statins was associated with culture establishment for IHD patients (p = 0.049 and p = 0.006, respectively). Conclusions: Together, these results show that the sternum bone can be used as a source for MSC isolation, and that ischemic or valvular diseases do not influence the cellular yield, culture establishment or in vitro growth kinetics.