Changes in glycosaminoglycans and proteoglycans of normal breast and fibroadenoma during the menstrual cycle
Autor(a) principal: | |
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Data de Publicação: | 2012 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNIFESP |
Texto Completo: | http://repositorio.unifesp.br/handle/11600/35010 http://dx.doi.org/10.1016/j.bbagen.2012.04.010 |
Resumo: | Background: Fibroadenoma is the most common breast tumor in young women, and its growth and metabolism may be under hormonal control. in the present paper we described the proteoglycan (PG) composition and synthesis rate of normal breast and fibroadenoma during the menstrual cycle.Methods: Samples of fibroadenoma and adjacent normal breast tissue were obtained at surgery. PGs were characterized by agarose gel electrophoresis and enzymatic degradation with glycosaminoglycan (GAG) lyases, and immunolocalized by confocal microscopy. To assess the synthesis rate, PGs were metabolic labeled by S-35-sulfate.Results: the concentration of PGs in normal breast was higher during the secretory phase. Fibroadenoma contained and synthesized more PGs than their paired controls, but the PG concentrations varied less with the menstrual cycle and, in contrast to normal tissue, peaked in the proliferative phase. the main mammary GAGs are heparan sulfate (HS, 71%-74%) and dermatan sulfate (DS, 26%-29%). the concentrations of both increased in fibroadenoma, but DS increased more, becoming 35%-37% of total. the DS chains contained more beta-D-glucuronic acid (IdoUA/GlcUA ratios were >10 in normal breast and 2-7 in fibroadenoma). the S-35-sulfate incorporation rate revealed that the in vitro synthesis rate of DS was higher than HS. Decorin was present in both tissues, while versican was found only in fibroadenoma.Conclusions: in normal breast, the PG concentration varied with the menstrual cycle. It was increased in fibroadenoma, especially DS.General significancePGs are increased in fibroadenoma, but their concentrations may be less sensitive to hormonal control. (C) 2012 Elsevier B.V. All rights reserved. |
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Lima, Cilene Reboucas de [UNIFESP]Santos Júnior, José de Arimatea [UNIFESP]Pinto Nazário, Afonso Celso [UNIFESP]Michelacci, Yara Maria [UNIFESP]Universidade Federal de São Paulo (UNIFESP)2016-01-24T14:27:23Z2016-01-24T14:27:23Z2012-07-01Biochimica Et Biophysica Acta-general Subjects. Amsterdam: Elsevier B.V., v. 1820, n. 7, p. 1009-1019, 2012.0304-4165http://repositorio.unifesp.br/handle/11600/35010http://dx.doi.org/10.1016/j.bbagen.2012.04.010WOS000305366100026.pdf10.1016/j.bbagen.2012.04.010WOS:000305366100026Background: Fibroadenoma is the most common breast tumor in young women, and its growth and metabolism may be under hormonal control. in the present paper we described the proteoglycan (PG) composition and synthesis rate of normal breast and fibroadenoma during the menstrual cycle.Methods: Samples of fibroadenoma and adjacent normal breast tissue were obtained at surgery. PGs were characterized by agarose gel electrophoresis and enzymatic degradation with glycosaminoglycan (GAG) lyases, and immunolocalized by confocal microscopy. To assess the synthesis rate, PGs were metabolic labeled by S-35-sulfate.Results: the concentration of PGs in normal breast was higher during the secretory phase. Fibroadenoma contained and synthesized more PGs than their paired controls, but the PG concentrations varied less with the menstrual cycle and, in contrast to normal tissue, peaked in the proliferative phase. the main mammary GAGs are heparan sulfate (HS, 71%-74%) and dermatan sulfate (DS, 26%-29%). the concentrations of both increased in fibroadenoma, but DS increased more, becoming 35%-37% of total. the DS chains contained more beta-D-glucuronic acid (IdoUA/GlcUA ratios were >10 in normal breast and 2-7 in fibroadenoma). the S-35-sulfate incorporation rate revealed that the in vitro synthesis rate of DS was higher than HS. Decorin was present in both tissues, while versican was found only in fibroadenoma.Conclusions: in normal breast, the PG concentration varied with the menstrual cycle. It was increased in fibroadenoma, especially DS.General significancePGs are increased in fibroadenoma, but their concentrations may be less sensitive to hormonal control. (C) 2012 Elsevier B.V. All rights reserved.Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Sociedade Paulista para o Desenvolvimento da Medicina (SPDM), São Paulo, SP, BrazilUniversidade Federal de São Paulo UNIFESP, Dept Bioquim, Escola Paulista Med, Disciplina Biol Mol, BR-04044020 São Paulo, BrazilUniversidade Federal de São Paulo UNIFESP, Dept Ginecol, Escola Paulista Med, BR-04044020 São Paulo, BrazilUniversidade Federal de São Paulo UNIFESP, Dept Bioquim, Escola Paulista Med, Disciplina Biol Mol, BR-04044020 São Paulo, BrazilUniversidade Federal de São Paulo UNIFESP, Dept Ginecol, Escola Paulista Med, BR-04044020 São Paulo, BrazilWeb of Science1009-1019engElsevier B.V.Biochimica Et Biophysica Acta-general Subjectshttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policyinfo:eu-repo/semantics/openAccessFibroadenomaProteoglycanMenstrual cycleNormal breastDecorinVersicanChanges in glycosaminoglycans and proteoglycans of normal breast and fibroadenoma during the menstrual cycleinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlereponame:Repositório Institucional da UNIFESPinstname:Universidade Federal de São Paulo (UNIFESP)instacron:UNIFESPORIGINALWOS000305366100026.pdfapplication/pdf2095267${dspace.ui.url}/bitstream/11600/35010/1/WOS000305366100026.pdf3a215f9f20a498ecf8a6511f116b030bMD51open accessTEXTWOS000305366100026.pdf.txtWOS000305366100026.pdf.txtExtracted texttext/plain53549${dspace.ui.url}/bitstream/11600/35010/2/WOS000305366100026.pdf.txtaea76448d1cf3b79b221148fbe32eb76MD52open access11600/350102021-09-29 14:54:12.608open accessoai:repositorio.unifesp.br:11600/35010Repositório InstitucionalPUBhttp://www.repositorio.unifesp.br/oai/requestopendoar:34652021-09-29T17:54:12Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)false |
dc.title.en.fl_str_mv |
Changes in glycosaminoglycans and proteoglycans of normal breast and fibroadenoma during the menstrual cycle |
title |
Changes in glycosaminoglycans and proteoglycans of normal breast and fibroadenoma during the menstrual cycle |
spellingShingle |
Changes in glycosaminoglycans and proteoglycans of normal breast and fibroadenoma during the menstrual cycle Lima, Cilene Reboucas de [UNIFESP] Fibroadenoma Proteoglycan Menstrual cycle Normal breast Decorin Versican |
title_short |
Changes in glycosaminoglycans and proteoglycans of normal breast and fibroadenoma during the menstrual cycle |
title_full |
Changes in glycosaminoglycans and proteoglycans of normal breast and fibroadenoma during the menstrual cycle |
title_fullStr |
Changes in glycosaminoglycans and proteoglycans of normal breast and fibroadenoma during the menstrual cycle |
title_full_unstemmed |
Changes in glycosaminoglycans and proteoglycans of normal breast and fibroadenoma during the menstrual cycle |
title_sort |
Changes in glycosaminoglycans and proteoglycans of normal breast and fibroadenoma during the menstrual cycle |
author |
Lima, Cilene Reboucas de [UNIFESP] |
author_facet |
Lima, Cilene Reboucas de [UNIFESP] Santos Júnior, José de Arimatea [UNIFESP] Pinto Nazário, Afonso Celso [UNIFESP] Michelacci, Yara Maria [UNIFESP] |
author_role |
author |
author2 |
Santos Júnior, José de Arimatea [UNIFESP] Pinto Nazário, Afonso Celso [UNIFESP] Michelacci, Yara Maria [UNIFESP] |
author2_role |
author author author |
dc.contributor.institution.none.fl_str_mv |
Universidade Federal de São Paulo (UNIFESP) |
dc.contributor.author.fl_str_mv |
Lima, Cilene Reboucas de [UNIFESP] Santos Júnior, José de Arimatea [UNIFESP] Pinto Nazário, Afonso Celso [UNIFESP] Michelacci, Yara Maria [UNIFESP] |
dc.subject.eng.fl_str_mv |
Fibroadenoma Proteoglycan Menstrual cycle Normal breast Decorin Versican |
topic |
Fibroadenoma Proteoglycan Menstrual cycle Normal breast Decorin Versican |
description |
Background: Fibroadenoma is the most common breast tumor in young women, and its growth and metabolism may be under hormonal control. in the present paper we described the proteoglycan (PG) composition and synthesis rate of normal breast and fibroadenoma during the menstrual cycle.Methods: Samples of fibroadenoma and adjacent normal breast tissue were obtained at surgery. PGs were characterized by agarose gel electrophoresis and enzymatic degradation with glycosaminoglycan (GAG) lyases, and immunolocalized by confocal microscopy. To assess the synthesis rate, PGs were metabolic labeled by S-35-sulfate.Results: the concentration of PGs in normal breast was higher during the secretory phase. Fibroadenoma contained and synthesized more PGs than their paired controls, but the PG concentrations varied less with the menstrual cycle and, in contrast to normal tissue, peaked in the proliferative phase. the main mammary GAGs are heparan sulfate (HS, 71%-74%) and dermatan sulfate (DS, 26%-29%). the concentrations of both increased in fibroadenoma, but DS increased more, becoming 35%-37% of total. the DS chains contained more beta-D-glucuronic acid (IdoUA/GlcUA ratios were >10 in normal breast and 2-7 in fibroadenoma). the S-35-sulfate incorporation rate revealed that the in vitro synthesis rate of DS was higher than HS. Decorin was present in both tissues, while versican was found only in fibroadenoma.Conclusions: in normal breast, the PG concentration varied with the menstrual cycle. It was increased in fibroadenoma, especially DS.General significancePGs are increased in fibroadenoma, but their concentrations may be less sensitive to hormonal control. (C) 2012 Elsevier B.V. All rights reserved. |
publishDate |
2012 |
dc.date.issued.fl_str_mv |
2012-07-01 |
dc.date.accessioned.fl_str_mv |
2016-01-24T14:27:23Z |
dc.date.available.fl_str_mv |
2016-01-24T14:27:23Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
Biochimica Et Biophysica Acta-general Subjects. Amsterdam: Elsevier B.V., v. 1820, n. 7, p. 1009-1019, 2012. |
dc.identifier.uri.fl_str_mv |
http://repositorio.unifesp.br/handle/11600/35010 http://dx.doi.org/10.1016/j.bbagen.2012.04.010 |
dc.identifier.issn.none.fl_str_mv |
0304-4165 |
dc.identifier.file.none.fl_str_mv |
WOS000305366100026.pdf |
dc.identifier.doi.none.fl_str_mv |
10.1016/j.bbagen.2012.04.010 |
dc.identifier.wos.none.fl_str_mv |
WOS:000305366100026 |
identifier_str_mv |
Biochimica Et Biophysica Acta-general Subjects. Amsterdam: Elsevier B.V., v. 1820, n. 7, p. 1009-1019, 2012. 0304-4165 WOS000305366100026.pdf 10.1016/j.bbagen.2012.04.010 WOS:000305366100026 |
url |
http://repositorio.unifesp.br/handle/11600/35010 http://dx.doi.org/10.1016/j.bbagen.2012.04.010 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.ispartof.none.fl_str_mv |
Biochimica Et Biophysica Acta-general Subjects |
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http://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy info:eu-repo/semantics/openAccess |
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http://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy |
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openAccess |
dc.format.none.fl_str_mv |
1009-1019 |
dc.publisher.none.fl_str_mv |
Elsevier B.V. |
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Elsevier B.V. |
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