Genotoxicity assessment of the antimalarial compound artesunate in somatic cells of mice
Autor(a) principal: | |
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Data de Publicação: | 2011 |
Outros Autores: | , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNIFESP |
Texto Completo: | http://repositorio.unifesp.br/handle/11600/33716 http://dx.doi.org/10.1016/j.fct.2011.03.016 |
Resumo: | Artesunate is a derivate of artemisinin that is both an antimalarial agent and acts cytotoxically on tumor cells. Despite its therapeutic use, its in vivo genotoxic potential has still not been evaluated. This study, therefore, was an investigation into the effects of a single oral administration of artesunate with an in vivo comet assay that analyzed leukocytes from peripheral blood and liver cells, and a micronucleus (MN) assay of bone marrow cells from male Swiss mice. the artesunate was administered by oral gavage at doses of 5, 50 and 100 mg/kg. Cytotoxicity was assessed by scoring 200 consecutive polychromatic (PCE) and normochromatic (NCE) erythrocytes (PCE/NCE ratio). the results demonstrate that artesunate induced significant DNA damage only in liver cells and that high doses of artesunate caused an increase in the mean number of micronucleated polychromatic erythrocytes (MNPCE). Under our experimental conditions, artesunate showed weak genotoxic effects at low doses and clastogenic effects at high doses. the PCE/NCE ratio indicated no cytotoxicity. the data obtained suggest caution about either continuous or high-dose use of artesunate by humans. (C) 2011 Elsevier B.V. All rights reserved. |
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Aquino, IvaniPerazzo, Fábio Ferreira [UNIFESP]Maistro, Edson LuisUniv Estadual PaulistaUniversidade de São Paulo (USP)Universidade Federal de São Paulo (UNIFESP)2016-01-24T14:16:46Z2016-01-24T14:16:46Z2011-06-01Food and Chemical Toxicology. Oxford: Pergamon-Elsevier B.V., v. 49, n. 6, p. 1335-1339, 2011.0278-6915http://repositorio.unifesp.br/handle/11600/33716http://dx.doi.org/10.1016/j.fct.2011.03.016WOS000291514800021.pdf10.1016/j.fct.2011.03.016WOS:000291514800021Artesunate is a derivate of artemisinin that is both an antimalarial agent and acts cytotoxically on tumor cells. Despite its therapeutic use, its in vivo genotoxic potential has still not been evaluated. This study, therefore, was an investigation into the effects of a single oral administration of artesunate with an in vivo comet assay that analyzed leukocytes from peripheral blood and liver cells, and a micronucleus (MN) assay of bone marrow cells from male Swiss mice. the artesunate was administered by oral gavage at doses of 5, 50 and 100 mg/kg. Cytotoxicity was assessed by scoring 200 consecutive polychromatic (PCE) and normochromatic (NCE) erythrocytes (PCE/NCE ratio). the results demonstrate that artesunate induced significant DNA damage only in liver cells and that high doses of artesunate caused an increase in the mean number of micronucleated polychromatic erythrocytes (MNPCE). Under our experimental conditions, artesunate showed weak genotoxic effects at low doses and clastogenic effects at high doses. the PCE/NCE ratio indicated no cytotoxicity. the data obtained suggest caution about either continuous or high-dose use of artesunate by humans. (C) 2011 Elsevier B.V. All rights reserved.Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Univ Estadual Paulista, UNESP, Fac Filosofia & Ciencias, Dept Fonoaudiol, BR-17525900 Marilia, SP, BrazilUniv Estadual Paulista, UNESP, Inst Biociencias, Programa Posgrad Biol Geral & Aplicada, BR-18618970 Botucatu, SP, BrazilUniversidade Federal de São Paulo Unifesp, Dept Ciencias Exatas & Terra, BR-09972270 Diadema, SP, BrazilUniversidade Federal de São Paulo Unifesp, Dept Ciencias Exatas & Terra, BR-09972270 Diadema, SP, BrazilCNPq: 306544/2006-7FAPESP: 2008/51175-7Web of Science1335-1339engElsevier B.V.Food and Chemical Toxicologyhttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policyinfo:eu-repo/semantics/openAccessArtesunateArtemisinin derivateMicronucleus test comet assayClastogenicityGenotoxicity assessment of the antimalarial compound artesunate in somatic cells of miceinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlereponame:Repositório Institucional da UNIFESPinstname:Universidade Federal de São Paulo (UNIFESP)instacron:UNIFESPORIGINALWOS000291514800021.pdfapplication/pdf237078${dspace.ui.url}/bitstream/11600/33716/1/WOS000291514800021.pdffeac988bc2644f664fa8d9ee9cd51113MD51open accessTEXTWOS000291514800021.pdf.txtWOS000291514800021.pdf.txtExtracted texttext/plain28979${dspace.ui.url}/bitstream/11600/33716/9/WOS000291514800021.pdf.txt6e6e3929af0ee86864da3f71a0d1a967MD59open accessTHUMBNAILWOS000291514800021.pdf.jpgWOS000291514800021.pdf.jpgIM Thumbnailimage/jpeg7162${dspace.ui.url}/bitstream/11600/33716/11/WOS000291514800021.pdf.jpge8a4e9edb8c645a9bf4b7f8c8ab08d47MD511open access11600/337162023-06-05 19:25:59.69open accessoai:repositorio.unifesp.br:11600/33716Repositório InstitucionalPUBhttp://www.repositorio.unifesp.br/oai/requestopendoar:34652023-06-05T22:25:59Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)false |
dc.title.en.fl_str_mv |
Genotoxicity assessment of the antimalarial compound artesunate in somatic cells of mice |
title |
Genotoxicity assessment of the antimalarial compound artesunate in somatic cells of mice |
spellingShingle |
Genotoxicity assessment of the antimalarial compound artesunate in somatic cells of mice Aquino, Ivani Artesunate Artemisinin derivate Micronucleus test comet assay Clastogenicity |
title_short |
Genotoxicity assessment of the antimalarial compound artesunate in somatic cells of mice |
title_full |
Genotoxicity assessment of the antimalarial compound artesunate in somatic cells of mice |
title_fullStr |
Genotoxicity assessment of the antimalarial compound artesunate in somatic cells of mice |
title_full_unstemmed |
Genotoxicity assessment of the antimalarial compound artesunate in somatic cells of mice |
title_sort |
Genotoxicity assessment of the antimalarial compound artesunate in somatic cells of mice |
author |
Aquino, Ivani |
author_facet |
Aquino, Ivani Perazzo, Fábio Ferreira [UNIFESP] Maistro, Edson Luis |
author_role |
author |
author2 |
Perazzo, Fábio Ferreira [UNIFESP] Maistro, Edson Luis |
author2_role |
author author |
dc.contributor.institution.none.fl_str_mv |
Univ Estadual Paulista Universidade de São Paulo (USP) Universidade Federal de São Paulo (UNIFESP) |
dc.contributor.author.fl_str_mv |
Aquino, Ivani Perazzo, Fábio Ferreira [UNIFESP] Maistro, Edson Luis |
dc.subject.eng.fl_str_mv |
Artesunate Artemisinin derivate Micronucleus test comet assay Clastogenicity |
topic |
Artesunate Artemisinin derivate Micronucleus test comet assay Clastogenicity |
description |
Artesunate is a derivate of artemisinin that is both an antimalarial agent and acts cytotoxically on tumor cells. Despite its therapeutic use, its in vivo genotoxic potential has still not been evaluated. This study, therefore, was an investigation into the effects of a single oral administration of artesunate with an in vivo comet assay that analyzed leukocytes from peripheral blood and liver cells, and a micronucleus (MN) assay of bone marrow cells from male Swiss mice. the artesunate was administered by oral gavage at doses of 5, 50 and 100 mg/kg. Cytotoxicity was assessed by scoring 200 consecutive polychromatic (PCE) and normochromatic (NCE) erythrocytes (PCE/NCE ratio). the results demonstrate that artesunate induced significant DNA damage only in liver cells and that high doses of artesunate caused an increase in the mean number of micronucleated polychromatic erythrocytes (MNPCE). Under our experimental conditions, artesunate showed weak genotoxic effects at low doses and clastogenic effects at high doses. the PCE/NCE ratio indicated no cytotoxicity. the data obtained suggest caution about either continuous or high-dose use of artesunate by humans. (C) 2011 Elsevier B.V. All rights reserved. |
publishDate |
2011 |
dc.date.issued.fl_str_mv |
2011-06-01 |
dc.date.accessioned.fl_str_mv |
2016-01-24T14:16:46Z |
dc.date.available.fl_str_mv |
2016-01-24T14:16:46Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
Food and Chemical Toxicology. Oxford: Pergamon-Elsevier B.V., v. 49, n. 6, p. 1335-1339, 2011. |
dc.identifier.uri.fl_str_mv |
http://repositorio.unifesp.br/handle/11600/33716 http://dx.doi.org/10.1016/j.fct.2011.03.016 |
dc.identifier.issn.none.fl_str_mv |
0278-6915 |
dc.identifier.file.none.fl_str_mv |
WOS000291514800021.pdf |
dc.identifier.doi.none.fl_str_mv |
10.1016/j.fct.2011.03.016 |
dc.identifier.wos.none.fl_str_mv |
WOS:000291514800021 |
identifier_str_mv |
Food and Chemical Toxicology. Oxford: Pergamon-Elsevier B.V., v. 49, n. 6, p. 1335-1339, 2011. 0278-6915 WOS000291514800021.pdf 10.1016/j.fct.2011.03.016 WOS:000291514800021 |
url |
http://repositorio.unifesp.br/handle/11600/33716 http://dx.doi.org/10.1016/j.fct.2011.03.016 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
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Food and Chemical Toxicology |
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http://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy info:eu-repo/semantics/openAccess |
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http://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy |
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openAccess |
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1335-1339 |
dc.publisher.none.fl_str_mv |
Elsevier B.V. |
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Elsevier B.V. |
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