Oligofructose supplementation during pregnancy and lactation impairs offspring development and alters the intestinal properties of 21-d-old pups
Autor(a) principal: | |
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Data de Publicação: | 2014 |
Outros Autores: | , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNIFESP |
Texto Completo: | http://repositorio.unifesp.br/handle/11600/37428 http://dx.doi.org/10.1186/1476-511X-13-26 |
Resumo: | Background: Previously, we showed that the intake of trans fatty acids during pregnancy and lactation triggers a pro-inflammatory status in the offspring. On the other hand, prebiotics can alter the intestinal environment, reducing serum lipopolysaccharides (LPS) concentrations. This study evaluated the effect of the oligofructose 10% diet supplementation in the presence or absence of hydrogenated vegetable fat during pregnancy and lactation on the development, endotoxemia and bacterial composition of 21-d-old offspring.Methods: On the first day of pregnancy rats were divided into four groups: control diet (C), control diet supplemented with 10% oligofructose (CF), diet enriched with hydrogenated vegetable fat, rich in TFA (T) or diet enriched with hydrogenated vegetable fat supplemented with 10% oligofructose (TF). Diets were maintained during pregnancy and lactation. At birth, 7th, 14th and 21th, pups were weighed and length was measured. Serum concentrations of LPS and free fatty acids (FFA) were performed by specific kits. Bacterial DNA present in faeces was determined by real-time PCR. Data were expressed as mean +/- standard error of the mean and the statistical analysis was realized by ANOVA two-way and ANOVA for repeated measures. p < 0.05 was considered significant.Results: We observed that the oligofructose (10%) supplementation during pregnancy and lactation reduced body weight, body weight gain, length and serum FFA in the CF and TF group compared to C and T group respectively, of the 21-day-old offspring, accompanied by an increase in serum LPS and genomic DNA levels of lactobacillus spp. on faeces of the CF group in relation to C group.Conclusion: in conclusion, dam's diet supplementation with 10% of oligofructose during pregnancy and lactation, independent of addition with hydrogenated vegetable fat, harms the offspring development, alters the bacterial composition and increases the serum concentrations of lipopolysaccharides in 21d-old pups. |
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Mennitti, Lais Vales [UNIFESP]Oyama, Lila Missae [UNIFESP]Oliveira, Juliana Lopez de [UNIFESP]Hachul, Ana Claudia Losinskas [UNIFESP]Santamarina, Aline Boveto [UNIFESP]Santana, Aline Alves de [UNIFESP]Okuda, Marcos Hiromu [UNIFESP]Ribeiro, Eliane Beraldi [UNIFESP]Nascimento, Claudia Maria da Penha Oller do [UNIFESP]Pisani, Luciana Pellegrini [UNIFESP]Universidade Federal de São Paulo (UNIFESP)2016-01-24T14:35:18Z2016-01-24T14:35:18Z2014-02-05Lipids in Health and Disease. London: Biomed Central Ltd, v. 13, 10 p., 2014.1476-511Xhttp://repositorio.unifesp.br/handle/11600/37428http://dx.doi.org/10.1186/1476-511X-13-26WOS000333647200002.pdf10.1186/1476-511X-13-26WOS:000333647200002Background: Previously, we showed that the intake of trans fatty acids during pregnancy and lactation triggers a pro-inflammatory status in the offspring. On the other hand, prebiotics can alter the intestinal environment, reducing serum lipopolysaccharides (LPS) concentrations. This study evaluated the effect of the oligofructose 10% diet supplementation in the presence or absence of hydrogenated vegetable fat during pregnancy and lactation on the development, endotoxemia and bacterial composition of 21-d-old offspring.Methods: On the first day of pregnancy rats were divided into four groups: control diet (C), control diet supplemented with 10% oligofructose (CF), diet enriched with hydrogenated vegetable fat, rich in TFA (T) or diet enriched with hydrogenated vegetable fat supplemented with 10% oligofructose (TF). Diets were maintained during pregnancy and lactation. At birth, 7th, 14th and 21th, pups were weighed and length was measured. Serum concentrations of LPS and free fatty acids (FFA) were performed by specific kits. Bacterial DNA present in faeces was determined by real-time PCR. Data were expressed as mean +/- standard error of the mean and the statistical analysis was realized by ANOVA two-way and ANOVA for repeated measures. p < 0.05 was considered significant.Results: We observed that the oligofructose (10%) supplementation during pregnancy and lactation reduced body weight, body weight gain, length and serum FFA in the CF and TF group compared to C and T group respectively, of the 21-day-old offspring, accompanied by an increase in serum LPS and genomic DNA levels of lactobacillus spp. on faeces of the CF group in relation to C group.Conclusion: in conclusion, dam's diet supplementation with 10% of oligofructose during pregnancy and lactation, independent of addition with hydrogenated vegetable fat, harms the offspring development, alters the bacterial composition and increases the serum concentrations of lipopolysaccharides in 21d-old pups.Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Universidade Federal de São Paulo, Programa Posgrad Interdisciplinar Ciencias Saude, Santos, SP, BrazilUniversidade Federal de São Paulo, Inst Saude & Soc, Dept Biociencias, Santos, SP, BrazilUniversidade Federal de São Paulo, Escola Paulista Med, Disciplina Fisiol Nutr, Dept Fisiol, São Paulo, BrazilUniversidade Federal de São Paulo, Programa Posgrad Interdisciplinar Ciencias Saude, Santos, SP, BrazilUniversidade Federal de São Paulo, Inst Saude & Soc, Dept Biociencias, Santos, SP, BrazilUniversidade Federal de São Paulo, Escola Paulista Med, Disciplina Fisiol Nutr, Dept Fisiol, São Paulo, BrazilFAPESP: 2011/19426-2Web of Science10engBiomed Central LtdLipids in Health and DiseaseHydrogenated vegetable fatOligofructoseCorporal compositionLipopolysaccharidesLactobacillus sppPregnancyLactationOligofructose supplementation during pregnancy and lactation impairs offspring development and alters the intestinal properties of 21-d-old pupsinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UNIFESPinstname:Universidade Federal de São Paulo (UNIFESP)instacron:UNIFESPORIGINALWOS000333647200002.pdfapplication/pdf669382${dspace.ui.url}/bitstream/11600/37428/1/WOS000333647200002.pdf0b485be798ffc39c8da8e9fa6fd99b4bMD51open accessTEXTWOS000333647200002.pdf.txtWOS000333647200002.pdf.txtExtracted texttext/plain49431${dspace.ui.url}/bitstream/11600/37428/6/WOS000333647200002.pdf.txt890a9e14656306d70cd099d1674a6f72MD56open accessTHUMBNAILWOS000333647200002.pdf.jpgWOS000333647200002.pdf.jpgIM Thumbnailimage/jpeg6668${dspace.ui.url}/bitstream/11600/37428/8/WOS000333647200002.pdf.jpg74cb025d538a22c6a09156bc03e5a6edMD58open access11600/374282023-06-05 19:09:12.629open accessoai:repositorio.unifesp.br:11600/37428Repositório InstitucionalPUBhttp://www.repositorio.unifesp.br/oai/requestopendoar:34652023-06-05T22:09:12Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)false |
dc.title.en.fl_str_mv |
Oligofructose supplementation during pregnancy and lactation impairs offspring development and alters the intestinal properties of 21-d-old pups |
title |
Oligofructose supplementation during pregnancy and lactation impairs offspring development and alters the intestinal properties of 21-d-old pups |
spellingShingle |
Oligofructose supplementation during pregnancy and lactation impairs offspring development and alters the intestinal properties of 21-d-old pups Mennitti, Lais Vales [UNIFESP] Hydrogenated vegetable fat Oligofructose Corporal composition Lipopolysaccharides Lactobacillus spp Pregnancy Lactation |
title_short |
Oligofructose supplementation during pregnancy and lactation impairs offspring development and alters the intestinal properties of 21-d-old pups |
title_full |
Oligofructose supplementation during pregnancy and lactation impairs offspring development and alters the intestinal properties of 21-d-old pups |
title_fullStr |
Oligofructose supplementation during pregnancy and lactation impairs offspring development and alters the intestinal properties of 21-d-old pups |
title_full_unstemmed |
Oligofructose supplementation during pregnancy and lactation impairs offspring development and alters the intestinal properties of 21-d-old pups |
title_sort |
Oligofructose supplementation during pregnancy and lactation impairs offspring development and alters the intestinal properties of 21-d-old pups |
author |
Mennitti, Lais Vales [UNIFESP] |
author_facet |
Mennitti, Lais Vales [UNIFESP] Oyama, Lila Missae [UNIFESP] Oliveira, Juliana Lopez de [UNIFESP] Hachul, Ana Claudia Losinskas [UNIFESP] Santamarina, Aline Boveto [UNIFESP] Santana, Aline Alves de [UNIFESP] Okuda, Marcos Hiromu [UNIFESP] Ribeiro, Eliane Beraldi [UNIFESP] Nascimento, Claudia Maria da Penha Oller do [UNIFESP] Pisani, Luciana Pellegrini [UNIFESP] |
author_role |
author |
author2 |
Oyama, Lila Missae [UNIFESP] Oliveira, Juliana Lopez de [UNIFESP] Hachul, Ana Claudia Losinskas [UNIFESP] Santamarina, Aline Boveto [UNIFESP] Santana, Aline Alves de [UNIFESP] Okuda, Marcos Hiromu [UNIFESP] Ribeiro, Eliane Beraldi [UNIFESP] Nascimento, Claudia Maria da Penha Oller do [UNIFESP] Pisani, Luciana Pellegrini [UNIFESP] |
author2_role |
author author author author author author author author author |
dc.contributor.institution.none.fl_str_mv |
Universidade Federal de São Paulo (UNIFESP) |
dc.contributor.author.fl_str_mv |
Mennitti, Lais Vales [UNIFESP] Oyama, Lila Missae [UNIFESP] Oliveira, Juliana Lopez de [UNIFESP] Hachul, Ana Claudia Losinskas [UNIFESP] Santamarina, Aline Boveto [UNIFESP] Santana, Aline Alves de [UNIFESP] Okuda, Marcos Hiromu [UNIFESP] Ribeiro, Eliane Beraldi [UNIFESP] Nascimento, Claudia Maria da Penha Oller do [UNIFESP] Pisani, Luciana Pellegrini [UNIFESP] |
dc.subject.eng.fl_str_mv |
Hydrogenated vegetable fat Oligofructose Corporal composition Lipopolysaccharides Lactobacillus spp Pregnancy Lactation |
topic |
Hydrogenated vegetable fat Oligofructose Corporal composition Lipopolysaccharides Lactobacillus spp Pregnancy Lactation |
description |
Background: Previously, we showed that the intake of trans fatty acids during pregnancy and lactation triggers a pro-inflammatory status in the offspring. On the other hand, prebiotics can alter the intestinal environment, reducing serum lipopolysaccharides (LPS) concentrations. This study evaluated the effect of the oligofructose 10% diet supplementation in the presence or absence of hydrogenated vegetable fat during pregnancy and lactation on the development, endotoxemia and bacterial composition of 21-d-old offspring.Methods: On the first day of pregnancy rats were divided into four groups: control diet (C), control diet supplemented with 10% oligofructose (CF), diet enriched with hydrogenated vegetable fat, rich in TFA (T) or diet enriched with hydrogenated vegetable fat supplemented with 10% oligofructose (TF). Diets were maintained during pregnancy and lactation. At birth, 7th, 14th and 21th, pups were weighed and length was measured. Serum concentrations of LPS and free fatty acids (FFA) were performed by specific kits. Bacterial DNA present in faeces was determined by real-time PCR. Data were expressed as mean +/- standard error of the mean and the statistical analysis was realized by ANOVA two-way and ANOVA for repeated measures. p < 0.05 was considered significant.Results: We observed that the oligofructose (10%) supplementation during pregnancy and lactation reduced body weight, body weight gain, length and serum FFA in the CF and TF group compared to C and T group respectively, of the 21-day-old offspring, accompanied by an increase in serum LPS and genomic DNA levels of lactobacillus spp. on faeces of the CF group in relation to C group.Conclusion: in conclusion, dam's diet supplementation with 10% of oligofructose during pregnancy and lactation, independent of addition with hydrogenated vegetable fat, harms the offspring development, alters the bacterial composition and increases the serum concentrations of lipopolysaccharides in 21d-old pups. |
publishDate |
2014 |
dc.date.issued.fl_str_mv |
2014-02-05 |
dc.date.accessioned.fl_str_mv |
2016-01-24T14:35:18Z |
dc.date.available.fl_str_mv |
2016-01-24T14:35:18Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
Lipids in Health and Disease. London: Biomed Central Ltd, v. 13, 10 p., 2014. |
dc.identifier.uri.fl_str_mv |
http://repositorio.unifesp.br/handle/11600/37428 http://dx.doi.org/10.1186/1476-511X-13-26 |
dc.identifier.issn.none.fl_str_mv |
1476-511X |
dc.identifier.file.none.fl_str_mv |
WOS000333647200002.pdf |
dc.identifier.doi.none.fl_str_mv |
10.1186/1476-511X-13-26 |
dc.identifier.wos.none.fl_str_mv |
WOS:000333647200002 |
identifier_str_mv |
Lipids in Health and Disease. London: Biomed Central Ltd, v. 13, 10 p., 2014. 1476-511X WOS000333647200002.pdf 10.1186/1476-511X-13-26 WOS:000333647200002 |
url |
http://repositorio.unifesp.br/handle/11600/37428 http://dx.doi.org/10.1186/1476-511X-13-26 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
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Lipids in Health and Disease |
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info:eu-repo/semantics/openAccess |
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openAccess |
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10 |
dc.publisher.none.fl_str_mv |
Biomed Central Ltd |
publisher.none.fl_str_mv |
Biomed Central Ltd |
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