Mycobacterium tuberculosis complex differentiation using gyrB-restriction fragment length polymorphism analysis
Autor(a) principal: | |
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Data de Publicação: | 2004 |
Outros Autores: | , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNIFESP |
Texto Completo: | http://repositorio.unifesp.br/handle/11600/2267 http://dx.doi.org/10.1590/S0074-02762004000700014 |
Resumo: | Mycobacterium tuberculosis complex (MTBC) members are causative agents of human and animal tuberculosis. Differentiation of MTBC members is required for appropriate treatment of individual patients and for epidemiological purposes. Strains from six MTBC species - M. tuberculosis, M. bovis subsp. bovis, M. bovis BCG, M. africanum, M. pinnipedii, and M. canetti - were studied using gyrB-restriction fragment length polymorphism (gyrB-RFLP) analysis. A table was elaborated, based on observed restriction patterns and published gyrB sequences. To evaluate applicability of gyrB-RFLP at Instituto Adolfo Lutz, São Paulo, Mycobacterial Reference Laboratory, 311 MTBC clinical isolates, previously identified using traditional methods as M. tuberculosis (306), M. bovis (3), and M. bovis BCG (2), were analyzed by gyrB-RFLP. All isolates were correctly identified by the molecular method, but no distinction between M. bovis and M. bovis BCG was obtained. Differentiation of M. tuberculosis and M. bovis is of utmost importance, because they require different treatment schedules. In conclusion, gyrB-RFLP is accurate and easy-to-perform, with potential to reduce time needed for conventional differentiation methods. However, application for epidemiological studies remains limited, because it cannot differentiate M. tuberculosis from M. africanum subtype II, and M. canetti, M. africanum subtype I from M. pinnipedii, and. M. bovis from M. bovis BCG. |
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Chimara, EricaFerrazoli, LucilaineLeao, Sylvia Cardoso [UNIFESP]Instituto Adolfo Lutz Seção de Bacteriologia Setor de MicobactériasUniversidade Federal de São Paulo (UNIFESP)2015-06-14T13:31:19Z2015-06-14T13:31:19Z2004-11-01Memórias do Instituto Oswaldo Cruz. Instituto Oswaldo Cruz, Ministério da Saúde, v. 99, n. 7, p. 745-748, 2004.0074-0276http://repositorio.unifesp.br/handle/11600/2267http://dx.doi.org/10.1590/S0074-02762004000700014S0074-02762004000700014.pdfS0074-0276200400070001410.1590/S0074-02762004000700014Mycobacterium tuberculosis complex (MTBC) members are causative agents of human and animal tuberculosis. Differentiation of MTBC members is required for appropriate treatment of individual patients and for epidemiological purposes. Strains from six MTBC species - M. tuberculosis, M. bovis subsp. bovis, M. bovis BCG, M. africanum, M. pinnipedii, and M. canetti - were studied using gyrB-restriction fragment length polymorphism (gyrB-RFLP) analysis. A table was elaborated, based on observed restriction patterns and published gyrB sequences. To evaluate applicability of gyrB-RFLP at Instituto Adolfo Lutz, São Paulo, Mycobacterial Reference Laboratory, 311 MTBC clinical isolates, previously identified using traditional methods as M. tuberculosis (306), M. bovis (3), and M. bovis BCG (2), were analyzed by gyrB-RFLP. All isolates were correctly identified by the molecular method, but no distinction between M. bovis and M. bovis BCG was obtained. Differentiation of M. tuberculosis and M. bovis is of utmost importance, because they require different treatment schedules. In conclusion, gyrB-RFLP is accurate and easy-to-perform, with potential to reduce time needed for conventional differentiation methods. However, application for epidemiological studies remains limited, because it cannot differentiate M. tuberculosis from M. africanum subtype II, and M. canetti, M. africanum subtype I from M. pinnipedii, and. M. bovis from M. bovis BCG.Instituto Adolfo Lutz Seção de Bacteriologia Setor de MicobactériasUniversidade Federal de São Paulo (UNIFESP) Escola Paulista de Medicina Departamento de Microbiologia, Imunologia e ParasitologiaUNIFESP, EPM, Depto. de Microbiologia, Imunologia e ParasitologiaSciELO745-748engInstituto Oswaldo Cruz, Ministério da SaúdeMemórias do Instituto Oswaldo CruzMycobacterium tuberculosis complexgyrBrestriction fragment length polymorphismMycobacterium tuberculosis complex differentiation using gyrB-restriction fragment length polymorphism analysisinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UNIFESPinstname:Universidade Federal de São Paulo (UNIFESP)instacron:UNIFESPORIGINALS0074-02762004000700014.pdfapplication/pdf47359${dspace.ui.url}/bitstream/11600/2267/1/S0074-02762004000700014.pdf1b05b1f928655b9a1ecc67ad539ee948MD51open accessTEXTS0074-02762004000700014.pdf.txtS0074-02762004000700014.pdf.txtExtracted texttext/plain16710${dspace.ui.url}/bitstream/11600/2267/2/S0074-02762004000700014.pdf.txtede67af9bf78c8df349f53e0c2fe5158MD52open access11600/22672022-02-08 11:53:19.003open accessoai:repositorio.unifesp.br:11600/2267Repositório InstitucionalPUBhttp://www.repositorio.unifesp.br/oai/requestopendoar:34652022-02-08T14:53:19Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)false |
dc.title.en.fl_str_mv |
Mycobacterium tuberculosis complex differentiation using gyrB-restriction fragment length polymorphism analysis |
title |
Mycobacterium tuberculosis complex differentiation using gyrB-restriction fragment length polymorphism analysis |
spellingShingle |
Mycobacterium tuberculosis complex differentiation using gyrB-restriction fragment length polymorphism analysis Chimara, Erica Mycobacterium tuberculosis complex gyrB restriction fragment length polymorphism |
title_short |
Mycobacterium tuberculosis complex differentiation using gyrB-restriction fragment length polymorphism analysis |
title_full |
Mycobacterium tuberculosis complex differentiation using gyrB-restriction fragment length polymorphism analysis |
title_fullStr |
Mycobacterium tuberculosis complex differentiation using gyrB-restriction fragment length polymorphism analysis |
title_full_unstemmed |
Mycobacterium tuberculosis complex differentiation using gyrB-restriction fragment length polymorphism analysis |
title_sort |
Mycobacterium tuberculosis complex differentiation using gyrB-restriction fragment length polymorphism analysis |
author |
Chimara, Erica |
author_facet |
Chimara, Erica Ferrazoli, Lucilaine Leao, Sylvia Cardoso [UNIFESP] |
author_role |
author |
author2 |
Ferrazoli, Lucilaine Leao, Sylvia Cardoso [UNIFESP] |
author2_role |
author author |
dc.contributor.institution.none.fl_str_mv |
Instituto Adolfo Lutz Seção de Bacteriologia Setor de Micobactérias Universidade Federal de São Paulo (UNIFESP) |
dc.contributor.author.fl_str_mv |
Chimara, Erica Ferrazoli, Lucilaine Leao, Sylvia Cardoso [UNIFESP] |
dc.subject.eng.fl_str_mv |
Mycobacterium tuberculosis complex gyrB restriction fragment length polymorphism |
topic |
Mycobacterium tuberculosis complex gyrB restriction fragment length polymorphism |
description |
Mycobacterium tuberculosis complex (MTBC) members are causative agents of human and animal tuberculosis. Differentiation of MTBC members is required for appropriate treatment of individual patients and for epidemiological purposes. Strains from six MTBC species - M. tuberculosis, M. bovis subsp. bovis, M. bovis BCG, M. africanum, M. pinnipedii, and M. canetti - were studied using gyrB-restriction fragment length polymorphism (gyrB-RFLP) analysis. A table was elaborated, based on observed restriction patterns and published gyrB sequences. To evaluate applicability of gyrB-RFLP at Instituto Adolfo Lutz, São Paulo, Mycobacterial Reference Laboratory, 311 MTBC clinical isolates, previously identified using traditional methods as M. tuberculosis (306), M. bovis (3), and M. bovis BCG (2), were analyzed by gyrB-RFLP. All isolates were correctly identified by the molecular method, but no distinction between M. bovis and M. bovis BCG was obtained. Differentiation of M. tuberculosis and M. bovis is of utmost importance, because they require different treatment schedules. In conclusion, gyrB-RFLP is accurate and easy-to-perform, with potential to reduce time needed for conventional differentiation methods. However, application for epidemiological studies remains limited, because it cannot differentiate M. tuberculosis from M. africanum subtype II, and M. canetti, M. africanum subtype I from M. pinnipedii, and. M. bovis from M. bovis BCG. |
publishDate |
2004 |
dc.date.issued.fl_str_mv |
2004-11-01 |
dc.date.accessioned.fl_str_mv |
2015-06-14T13:31:19Z |
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2015-06-14T13:31:19Z |
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info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
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article |
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dc.identifier.citation.fl_str_mv |
Memórias do Instituto Oswaldo Cruz. Instituto Oswaldo Cruz, Ministério da Saúde, v. 99, n. 7, p. 745-748, 2004. |
dc.identifier.uri.fl_str_mv |
http://repositorio.unifesp.br/handle/11600/2267 http://dx.doi.org/10.1590/S0074-02762004000700014 |
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0074-0276 |
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S0074-02762004000700014.pdf |
dc.identifier.scielo.none.fl_str_mv |
S0074-02762004000700014 |
dc.identifier.doi.none.fl_str_mv |
10.1590/S0074-02762004000700014 |
identifier_str_mv |
Memórias do Instituto Oswaldo Cruz. Instituto Oswaldo Cruz, Ministério da Saúde, v. 99, n. 7, p. 745-748, 2004. 0074-0276 S0074-02762004000700014.pdf S0074-02762004000700014 10.1590/S0074-02762004000700014 |
url |
http://repositorio.unifesp.br/handle/11600/2267 http://dx.doi.org/10.1590/S0074-02762004000700014 |
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eng |
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Memórias do Instituto Oswaldo Cruz |
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745-748 |
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Instituto Oswaldo Cruz, Ministério da Saúde |
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Instituto Oswaldo Cruz, Ministério da Saúde |
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