Molecular cloning of ion channels in Felis catus that are related to periodic paralyses in man: a contribution to the understanding of the genetic susceptibility to feline neck ventroflexion and paralysis
Autor(a) principal: | |
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Data de Publicação: | 2014 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNIFESP |
Texto Completo: | https://repositorio.unifesp.br/handle/11600/38217 https://dx.doi.org/10.1242/bio.20148003 |
Resumo: | Neck ventroflexion in cats has different causes; however, the most common is the hypokalemia associated with flaccid paralysis secondary to chronic renal failure. in humans, the most common causes of acute flaccid paralysis are hypokalemia precipitated by thyrotoxicosis and familial forms linked to mutations in sodium, potassium, and calcium channel genes. Here, we describe the sequencing and analysis of skeletal muscle ion channels in Felis catus that could be related to periodic paralyses in humans, contributing to the understanding of the genetic susceptibility to feline neck ventroflexion and paralysis. We studied genomic DNA from eleven cats, including five animals that were hyperthyroid with hypokalemia, although only one presented with muscle weakness, and six healthy control domestic cats. We identified the ion channel ortholog genes KCNJ2, KCNJ12, KCNJ14, CACNA1S and SCN4A in the Felis catus genome, together with several polymorphic variants. Upon comparative alignment with other genomes, we found that Felis catus provides evidence for a high genomic conservation of ion channel sequences. Although we hypothesized that neck ventroflexion in cats could be associated with a thyrotoxic or familial periodic paralysis channel mutation, we did not identify any previously detected human channel mutation in the hyperthyroid cat presenting hypokalemia. However, based on the small number of affected cats in this study, we cannot yet rule out this molecular mechanism. Notwithstanding, hyperthyroidism should still be considered as a differential diagnosis in hypokalemic feline paralysis. |
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Zapata, Marlyn [UNIFESP]Kunii, Ilda Sizue [UNIFESP]Paninka, Rolf Matias [UNIFESP]Simoes, Denise M. N. [UNIFESP]Castillo, Victor A. [UNIFESP]Reche, Archivaldo [UNIFESP]Maciel, Rui Monteiro de Barros [UNIFESP]Dias-da-Silva, Magnus Régios [UNIFESP]Universidade Federal de São Paulo (UNIFESP)2016-01-24T14:37:52Z2016-01-24T14:37:52Z2014-09-15Biology Open. Cambridge: Company of Biologists Ltd, v. 3, n. 9, p. 785-793, 2014.2046-6390https://repositorio.unifesp.br/handle/11600/38217https://dx.doi.org/10.1242/bio.20148003WOS000348097400001.pdf10.1242/bio.20148003WOS:000348097400001Neck ventroflexion in cats has different causes; however, the most common is the hypokalemia associated with flaccid paralysis secondary to chronic renal failure. in humans, the most common causes of acute flaccid paralysis are hypokalemia precipitated by thyrotoxicosis and familial forms linked to mutations in sodium, potassium, and calcium channel genes. Here, we describe the sequencing and analysis of skeletal muscle ion channels in Felis catus that could be related to periodic paralyses in humans, contributing to the understanding of the genetic susceptibility to feline neck ventroflexion and paralysis. We studied genomic DNA from eleven cats, including five animals that were hyperthyroid with hypokalemia, although only one presented with muscle weakness, and six healthy control domestic cats. We identified the ion channel ortholog genes KCNJ2, KCNJ12, KCNJ14, CACNA1S and SCN4A in the Felis catus genome, together with several polymorphic variants. Upon comparative alignment with other genomes, we found that Felis catus provides evidence for a high genomic conservation of ion channel sequences. Although we hypothesized that neck ventroflexion in cats could be associated with a thyrotoxic or familial periodic paralysis channel mutation, we did not identify any previously detected human channel mutation in the hyperthyroid cat presenting hypokalemia. However, based on the small number of affected cats in this study, we cannot yet rule out this molecular mechanism. Notwithstanding, hyperthyroidism should still be considered as a differential diagnosis in hypokalemic feline paralysis.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Universidade Federal de São Paulo, Escola Paulista Med, Dept Med, Div Endocrinol,Lab Mol & Translat Endocrinol, BR-04039032 São Paulo, BrazilUniversidade Federal de São Paulo, Escola Paulista Med, Dept Med, Div Endocrinol,Lab Mol & Translat Endocrinol, BR-04039032 São Paulo, BrazilFAPESP: 2011/20747-8FAPESP: 2012/02529Web of Science785-793engCompany of Biologists LtdBiology OpenPotassium channelInward rectifierFelis catusKir2.xKCNJ2KCNJ12KCNJ18CACNA1SSCN4ACatMolecular cloning of ion channels in Felis catus that are related to periodic paralyses in man: a contribution to the understanding of the genetic susceptibility to feline neck ventroflexion and paralysisinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UNIFESPinstname:Universidade Federal de São Paulo (UNIFESP)instacron:UNIFESPORIGINALWOS000348097400001.pdfapplication/pdf824428${dspace.ui.url}/bitstream/11600/38217/1/WOS000348097400001.pdfaa46af58b2ec629c6d916599e0d67254MD51open accessTEXTWOS000348097400001.pdf.txtWOS000348097400001.pdf.txtExtracted texttext/plain43652${dspace.ui.url}/bitstream/11600/38217/2/WOS000348097400001.pdf.txtb65ecbd9221053596f5d57b2ff852797MD52open access11600/382172023-08-14 21:13:24.714open accessoai:repositorio.unifesp.br:11600/38217Repositório InstitucionalPUBhttp://www.repositorio.unifesp.br/oai/requestopendoar:34652023-08-15T00:13:24Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)false |
dc.title.en.fl_str_mv |
Molecular cloning of ion channels in Felis catus that are related to periodic paralyses in man: a contribution to the understanding of the genetic susceptibility to feline neck ventroflexion and paralysis |
title |
Molecular cloning of ion channels in Felis catus that are related to periodic paralyses in man: a contribution to the understanding of the genetic susceptibility to feline neck ventroflexion and paralysis |
spellingShingle |
Molecular cloning of ion channels in Felis catus that are related to periodic paralyses in man: a contribution to the understanding of the genetic susceptibility to feline neck ventroflexion and paralysis Zapata, Marlyn [UNIFESP] Potassium channel Inward rectifier Felis catus Kir2.x KCNJ2 KCNJ12 KCNJ18 CACNA1S SCN4A Cat |
title_short |
Molecular cloning of ion channels in Felis catus that are related to periodic paralyses in man: a contribution to the understanding of the genetic susceptibility to feline neck ventroflexion and paralysis |
title_full |
Molecular cloning of ion channels in Felis catus that are related to periodic paralyses in man: a contribution to the understanding of the genetic susceptibility to feline neck ventroflexion and paralysis |
title_fullStr |
Molecular cloning of ion channels in Felis catus that are related to periodic paralyses in man: a contribution to the understanding of the genetic susceptibility to feline neck ventroflexion and paralysis |
title_full_unstemmed |
Molecular cloning of ion channels in Felis catus that are related to periodic paralyses in man: a contribution to the understanding of the genetic susceptibility to feline neck ventroflexion and paralysis |
title_sort |
Molecular cloning of ion channels in Felis catus that are related to periodic paralyses in man: a contribution to the understanding of the genetic susceptibility to feline neck ventroflexion and paralysis |
author |
Zapata, Marlyn [UNIFESP] |
author_facet |
Zapata, Marlyn [UNIFESP] Kunii, Ilda Sizue [UNIFESP] Paninka, Rolf Matias [UNIFESP] Simoes, Denise M. N. [UNIFESP] Castillo, Victor A. [UNIFESP] Reche, Archivaldo [UNIFESP] Maciel, Rui Monteiro de Barros [UNIFESP] Dias-da-Silva, Magnus Régios [UNIFESP] |
author_role |
author |
author2 |
Kunii, Ilda Sizue [UNIFESP] Paninka, Rolf Matias [UNIFESP] Simoes, Denise M. N. [UNIFESP] Castillo, Victor A. [UNIFESP] Reche, Archivaldo [UNIFESP] Maciel, Rui Monteiro de Barros [UNIFESP] Dias-da-Silva, Magnus Régios [UNIFESP] |
author2_role |
author author author author author author author |
dc.contributor.institution.none.fl_str_mv |
Universidade Federal de São Paulo (UNIFESP) |
dc.contributor.author.fl_str_mv |
Zapata, Marlyn [UNIFESP] Kunii, Ilda Sizue [UNIFESP] Paninka, Rolf Matias [UNIFESP] Simoes, Denise M. N. [UNIFESP] Castillo, Victor A. [UNIFESP] Reche, Archivaldo [UNIFESP] Maciel, Rui Monteiro de Barros [UNIFESP] Dias-da-Silva, Magnus Régios [UNIFESP] |
dc.subject.eng.fl_str_mv |
Potassium channel Inward rectifier Felis catus Kir2.x KCNJ2 KCNJ12 KCNJ18 CACNA1S SCN4A Cat |
topic |
Potassium channel Inward rectifier Felis catus Kir2.x KCNJ2 KCNJ12 KCNJ18 CACNA1S SCN4A Cat |
description |
Neck ventroflexion in cats has different causes; however, the most common is the hypokalemia associated with flaccid paralysis secondary to chronic renal failure. in humans, the most common causes of acute flaccid paralysis are hypokalemia precipitated by thyrotoxicosis and familial forms linked to mutations in sodium, potassium, and calcium channel genes. Here, we describe the sequencing and analysis of skeletal muscle ion channels in Felis catus that could be related to periodic paralyses in humans, contributing to the understanding of the genetic susceptibility to feline neck ventroflexion and paralysis. We studied genomic DNA from eleven cats, including five animals that were hyperthyroid with hypokalemia, although only one presented with muscle weakness, and six healthy control domestic cats. We identified the ion channel ortholog genes KCNJ2, KCNJ12, KCNJ14, CACNA1S and SCN4A in the Felis catus genome, together with several polymorphic variants. Upon comparative alignment with other genomes, we found that Felis catus provides evidence for a high genomic conservation of ion channel sequences. Although we hypothesized that neck ventroflexion in cats could be associated with a thyrotoxic or familial periodic paralysis channel mutation, we did not identify any previously detected human channel mutation in the hyperthyroid cat presenting hypokalemia. However, based on the small number of affected cats in this study, we cannot yet rule out this molecular mechanism. Notwithstanding, hyperthyroidism should still be considered as a differential diagnosis in hypokalemic feline paralysis. |
publishDate |
2014 |
dc.date.issued.fl_str_mv |
2014-09-15 |
dc.date.accessioned.fl_str_mv |
2016-01-24T14:37:52Z |
dc.date.available.fl_str_mv |
2016-01-24T14:37:52Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
Biology Open. Cambridge: Company of Biologists Ltd, v. 3, n. 9, p. 785-793, 2014. |
dc.identifier.uri.fl_str_mv |
https://repositorio.unifesp.br/handle/11600/38217 https://dx.doi.org/10.1242/bio.20148003 |
dc.identifier.issn.none.fl_str_mv |
2046-6390 |
dc.identifier.file.none.fl_str_mv |
WOS000348097400001.pdf |
dc.identifier.doi.none.fl_str_mv |
10.1242/bio.20148003 |
dc.identifier.wos.none.fl_str_mv |
WOS:000348097400001 |
identifier_str_mv |
Biology Open. Cambridge: Company of Biologists Ltd, v. 3, n. 9, p. 785-793, 2014. 2046-6390 WOS000348097400001.pdf 10.1242/bio.20148003 WOS:000348097400001 |
url |
https://repositorio.unifesp.br/handle/11600/38217 https://dx.doi.org/10.1242/bio.20148003 |
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eng |
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Biology Open |
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785-793 |
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Company of Biologists Ltd |
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Company of Biologists Ltd |
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reponame:Repositório Institucional da UNIFESP instname:Universidade Federal de São Paulo (UNIFESP) instacron:UNIFESP |
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