Expression of the selectable marker gene bsrm in BALB/MK cells induces apoptosis by overproduction of hydrogen peroxide
Autor(a) principal: | |
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Data de Publicação: | 2007 |
Outros Autores: | , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNIFESP |
Texto Completo: | http://repositorio.unifesp.br/handle/11600/30058 http://dx.doi.org/10.1139/O07-058 |
Resumo: | Transduction of the retroviral vector LBmSN, which expresses the blasticidin S resistance gene bsrm in the murine keratinocyte cell line BALB/MK, induces death in these cells. Cell death is caused by a factor called DOKEB (death factor obtained from keratinocytes expressing bsrm), which is released before the cells' death. in this report we describe and discuss the purification and characterization of DOKEB. Our results were as follows. (i) the 5-day-old medium from the modified BALB/MK cells with LBmSN was used for purification and characterization by filtration and chromatography: DOKEB was a stable and highly hydrophilic compound, with a molecular mass less than that of I amino acid. (ii) the conditioned medium containing DOKEB was reactive against thiobarbituric acid and dichlorofluorescein diacetate. (iii) DOKEB activity was neutralized by the incubation of the conditioned medium with catalase. Therefore, our conclusion is that the BALB/MK cells expressing bsrm produce a large amount of hydrogen peroxide, which catalyzes the process of apoptosis of those cells. |
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Repositório Institucional da UNIFESP |
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Takeshita, DanielaBento, Fernanda MaraChammas, RogerBelizario, Jose ErnestoCarmona, Adriana KaraoglanovicKonno, KatsuhiroMelo, Robson Lopes deMolina, GustavoLisboa, Bianca Cristina GarciaHan, Sang Won [UNIFESP]Universidade Federal de São Paulo (UNIFESP)Universidade de São Paulo (USP)Butantan Inst2016-01-24T13:49:06Z2016-01-24T13:49:06Z2007-10-01Biochemistry and Cell Biology-biochimie Et Biologie Cellulaire. Ottawa: Natl Research Council Canada-n R C Research Press, v. 85, n. 5, p. 573-581, 2007.0829-8211http://repositorio.unifesp.br/handle/11600/30058http://dx.doi.org/10.1139/O07-05810.1139/O07-058WOS:000250624500005Transduction of the retroviral vector LBmSN, which expresses the blasticidin S resistance gene bsrm in the murine keratinocyte cell line BALB/MK, induces death in these cells. Cell death is caused by a factor called DOKEB (death factor obtained from keratinocytes expressing bsrm), which is released before the cells' death. in this report we describe and discuss the purification and characterization of DOKEB. Our results were as follows. (i) the 5-day-old medium from the modified BALB/MK cells with LBmSN was used for purification and characterization by filtration and chromatography: DOKEB was a stable and highly hydrophilic compound, with a molecular mass less than that of I amino acid. (ii) the conditioned medium containing DOKEB was reactive against thiobarbituric acid and dichlorofluorescein diacetate. (iii) DOKEB activity was neutralized by the incubation of the conditioned medium with catalase. Therefore, our conclusion is that the BALB/MK cells expressing bsrm produce a large amount of hydrogen peroxide, which catalyzes the process of apoptosis of those cells.Universidade Federal de São Paulo, Dept Biophys, São Paulo, BrazilUniversidade Federal de São Paulo, Interdisciplinary Ctr Gene Therapy, São Paulo, BrazilUniv São Paulo, Fac Med, Dept Radiol, São Paulo, BrazilUniv São Paulo, ICB, Dept Pharmacol, BR-09500900 São Paulo, BrazilButantan Inst, São Paulo, BrazilUniversidade Federal de São Paulo, Dept Biophys, São Paulo, BrazilUniversidade Federal de São Paulo, Interdisciplinary Ctr Gene Therapy, São Paulo, BrazilWeb of Science573-581engNatl Research Council Canada-n R C Research PressBiochemistry and Cell Biology-biochimie Et Biologie Cellulaireselectable markerapoptosishydrogen peroxideblasticidin S.Expression of the selectable marker gene bsrm in BALB/MK cells induces apoptosis by overproduction of hydrogen peroxideinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UNIFESPinstname:Universidade Federal de São Paulo (UNIFESP)instacron:UNIFESP11600/300582022-06-02 09:02:19.27metadata only accessoai:repositorio.unifesp.br:11600/30058Repositório InstitucionalPUBhttp://www.repositorio.unifesp.br/oai/requestopendoar:34652022-06-02T12:02:19Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)false |
dc.title.en.fl_str_mv |
Expression of the selectable marker gene bsrm in BALB/MK cells induces apoptosis by overproduction of hydrogen peroxide |
title |
Expression of the selectable marker gene bsrm in BALB/MK cells induces apoptosis by overproduction of hydrogen peroxide |
spellingShingle |
Expression of the selectable marker gene bsrm in BALB/MK cells induces apoptosis by overproduction of hydrogen peroxide Takeshita, Daniela selectable marker apoptosis hydrogen peroxide blasticidin S. |
title_short |
Expression of the selectable marker gene bsrm in BALB/MK cells induces apoptosis by overproduction of hydrogen peroxide |
title_full |
Expression of the selectable marker gene bsrm in BALB/MK cells induces apoptosis by overproduction of hydrogen peroxide |
title_fullStr |
Expression of the selectable marker gene bsrm in BALB/MK cells induces apoptosis by overproduction of hydrogen peroxide |
title_full_unstemmed |
Expression of the selectable marker gene bsrm in BALB/MK cells induces apoptosis by overproduction of hydrogen peroxide |
title_sort |
Expression of the selectable marker gene bsrm in BALB/MK cells induces apoptosis by overproduction of hydrogen peroxide |
author |
Takeshita, Daniela |
author_facet |
Takeshita, Daniela Bento, Fernanda Mara Chammas, Roger Belizario, Jose Ernesto Carmona, Adriana Karaoglanovic Konno, Katsuhiro Melo, Robson Lopes de Molina, Gustavo Lisboa, Bianca Cristina Garcia Han, Sang Won [UNIFESP] |
author_role |
author |
author2 |
Bento, Fernanda Mara Chammas, Roger Belizario, Jose Ernesto Carmona, Adriana Karaoglanovic Konno, Katsuhiro Melo, Robson Lopes de Molina, Gustavo Lisboa, Bianca Cristina Garcia Han, Sang Won [UNIFESP] |
author2_role |
author author author author author author author author author |
dc.contributor.institution.none.fl_str_mv |
Universidade Federal de São Paulo (UNIFESP) Universidade de São Paulo (USP) Butantan Inst |
dc.contributor.author.fl_str_mv |
Takeshita, Daniela Bento, Fernanda Mara Chammas, Roger Belizario, Jose Ernesto Carmona, Adriana Karaoglanovic Konno, Katsuhiro Melo, Robson Lopes de Molina, Gustavo Lisboa, Bianca Cristina Garcia Han, Sang Won [UNIFESP] |
dc.subject.eng.fl_str_mv |
selectable marker apoptosis hydrogen peroxide blasticidin S. |
topic |
selectable marker apoptosis hydrogen peroxide blasticidin S. |
description |
Transduction of the retroviral vector LBmSN, which expresses the blasticidin S resistance gene bsrm in the murine keratinocyte cell line BALB/MK, induces death in these cells. Cell death is caused by a factor called DOKEB (death factor obtained from keratinocytes expressing bsrm), which is released before the cells' death. in this report we describe and discuss the purification and characterization of DOKEB. Our results were as follows. (i) the 5-day-old medium from the modified BALB/MK cells with LBmSN was used for purification and characterization by filtration and chromatography: DOKEB was a stable and highly hydrophilic compound, with a molecular mass less than that of I amino acid. (ii) the conditioned medium containing DOKEB was reactive against thiobarbituric acid and dichlorofluorescein diacetate. (iii) DOKEB activity was neutralized by the incubation of the conditioned medium with catalase. Therefore, our conclusion is that the BALB/MK cells expressing bsrm produce a large amount of hydrogen peroxide, which catalyzes the process of apoptosis of those cells. |
publishDate |
2007 |
dc.date.issued.fl_str_mv |
2007-10-01 |
dc.date.accessioned.fl_str_mv |
2016-01-24T13:49:06Z |
dc.date.available.fl_str_mv |
2016-01-24T13:49:06Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
Biochemistry and Cell Biology-biochimie Et Biologie Cellulaire. Ottawa: Natl Research Council Canada-n R C Research Press, v. 85, n. 5, p. 573-581, 2007. |
dc.identifier.uri.fl_str_mv |
http://repositorio.unifesp.br/handle/11600/30058 http://dx.doi.org/10.1139/O07-058 |
dc.identifier.issn.none.fl_str_mv |
0829-8211 |
dc.identifier.doi.none.fl_str_mv |
10.1139/O07-058 |
dc.identifier.wos.none.fl_str_mv |
WOS:000250624500005 |
identifier_str_mv |
Biochemistry and Cell Biology-biochimie Et Biologie Cellulaire. Ottawa: Natl Research Council Canada-n R C Research Press, v. 85, n. 5, p. 573-581, 2007. 0829-8211 10.1139/O07-058 WOS:000250624500005 |
url |
http://repositorio.unifesp.br/handle/11600/30058 http://dx.doi.org/10.1139/O07-058 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.ispartof.none.fl_str_mv |
Biochemistry and Cell Biology-biochimie Et Biologie Cellulaire |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
573-581 |
dc.publisher.none.fl_str_mv |
Natl Research Council Canada-n R C Research Press |
publisher.none.fl_str_mv |
Natl Research Council Canada-n R C Research Press |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da UNIFESP instname:Universidade Federal de São Paulo (UNIFESP) instacron:UNIFESP |
instname_str |
Universidade Federal de São Paulo (UNIFESP) |
instacron_str |
UNIFESP |
institution |
UNIFESP |
reponame_str |
Repositório Institucional da UNIFESP |
collection |
Repositório Institucional da UNIFESP |
repository.name.fl_str_mv |
Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP) |
repository.mail.fl_str_mv |
|
_version_ |
1802764129361985536 |