A Daily Dose of 5 mg Folic Acid for 90 Days Is Associated with Increased Serum Unmetabolized Folic Acid and Reduced Natural Killer Cell Cytotoxicity in Healthy Brazilian Adults
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2017 |
| Outros Autores: | , , , , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da UNIFESP |
| Texto Completo: | http://dx.doi.org/10.3945/jn.117.247445 http://repositorio.unifesp.br/handle/11600/51271 |
Resumo: | Background: The effects of high-dose folic acid (FA) supplementation in healthy individuals on blood folate concentrations and immune response are unknown. Objective: The aim of the study was to evaluate the effects of daily consumption of a tablet containing 5 mg FA on serum folate |
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A Daily Dose of 5 mg Folic Acid for 90 Days Is Associated with Increased Serum Unmetabolized Folic Acid and Reduced Natural Killer Cell Cytotoxicity in Healthy Brazilian Adultsfolic acid supplementationunmetabolized folic acidNK cell cytotoxicitymRNA expressionserum folate5-methyl-THFBackground: The effects of high-dose folic acid (FA) supplementation in healthy individuals on blood folate concentrations and immune response are unknown. Objective: The aim of the study was to evaluate the effects of daily consumption of a tablet containing 5 mg FA on serum folatenumber and cytotoxicity of natural killer (NK) cellsmRNA expression of dihydrofolate reductase (DHFR), methylenetetrahydrofolate reductase (MTHFR), interferon gamma (IFNG), tumor necrosis factor a (TNFA), and interleukin 8 (IL8) genesand concentrations of serum inflammatory markers. Methods: This prospective clinical trial was conducted in 30 healthy Brazilian adults (15 women), aged 27.7 y (95% CI: 26.4, 29.1 y), with a body mass index (in kg/m 2) of 23.1 (95% CI: 22.0, 24.3). Blood was collected at baseline and after 45 and 90 d of the intervention. Serum folate concentrations were measured by microbiological assay and HPLC-tandem mass spectrometry [ folate forms, including unmetabolized folic acid (UMFA)]. We used real-time polymerase chain reaction to assess mononuclear leukocyte mRNA expression and flow cytometry to measure the number and cytotoxicity of NK cells. Results: Serum folate concentrations increased by5-fold after the intervention (P < 0.001), and UMFA concentrations increased by 11.9-and 5.9-fold at 45 and 90 d, respectively, when compared with baseline (P < 0.001). UMFA concentrations increased (> 1.12 nmol/L) in 29 (96.6%) participants at day 45 and in 26 (86.7%) participants at day 90. We observed significant reductions in the number (P < 0.001) and cytotoxicity (P = 0.003) of NK cells after 45 and 90 d. Compared with baseline, DHFR mRNA expression was higher at 90 d (P = 0.006) and IL8 and TNFA mRNA expressions were higher at 45 and 90 d (P = 0.001 for both). Conclusion: This noncontrolled intervention showed that healthy adults responded to a high-dose FA supplement with increased UMFA concentrations, changes in cytokine mRNA expression, and reduced number and cytotoxicity of NK cells.Univ São Paulo, Dept Clin & Toxicol Anal, Fac Pharmaceut Sci, São Paulo, BrazilUniv São Paulo, Dept Food & Expt Nutr, Fac Pharmaceut Sci, São Paulo, BrazilUniv Fed São Paulo, Hematol & Blood Transfus Div, São Paulo, BrazilCDC, Natl Ctr Environm Hlth, Atlanta, GA 30333 USAUC Davis Sch Med, Dept Pathol & Lab Med, Sacramento, CA USAUniv Fed São Paulo, Hematol & Blood Transfus Div, São Paulo, BrazilWeb of ScienceFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)FAPESP: 2012/12912-1CNPq: 482641/2012-6CNPq: 401586/2014-6Amer Soc Nutrition-Asn2019-08-19T11:48:31Z2019-08-19T11:48:31Z2017info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersion1684-1692http://dx.doi.org/10.3945/jn.117.247445Journal Of Nutrition. Bethesda, v. 147, n. 9, p. 1684-1692, 2017.10.3945/jn.117.2474450022-3166http://repositorio.unifesp.br/handle/11600/51271WOS:000411807800012enginfo:eu-repo/semantics/openAccessPaniz, ClovisBertinato, Juliano FelixLucena, Maylla Rodrigues [UNIFESP]De Carli, Eduardoda Silva Amorim, Patricia MendoncaGomes, Guilherme WataruPalchetti, Cecilia ZaninFigueiredo, Maria Stella [UNIFESP]Pfeiffer, Christine M.Fazili, ZiaGreen, RalphGuerra-Shinohara, Elvira Maria [UNIFESP]reponame:Repositório Institucional da UNIFESPinstname:Universidade Federal de São Paulo (UNIFESP)instacron:UNIFESP2022-02-10T17:44:19Zoai:repositorio.unifesp.br/:11600/51271Repositório InstitucionalPUBhttp://www.repositorio.unifesp.br/oai/requestbiblioteca.csp@unifesp.bropendoar:34652022-02-10T17:44:19Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)false |
| dc.title.none.fl_str_mv |
A Daily Dose of 5 mg Folic Acid for 90 Days Is Associated with Increased Serum Unmetabolized Folic Acid and Reduced Natural Killer Cell Cytotoxicity in Healthy Brazilian Adults |
| title |
A Daily Dose of 5 mg Folic Acid for 90 Days Is Associated with Increased Serum Unmetabolized Folic Acid and Reduced Natural Killer Cell Cytotoxicity in Healthy Brazilian Adults |
| spellingShingle |
A Daily Dose of 5 mg Folic Acid for 90 Days Is Associated with Increased Serum Unmetabolized Folic Acid and Reduced Natural Killer Cell Cytotoxicity in Healthy Brazilian Adults Paniz, Clovis folic acid supplementation unmetabolized folic acid NK cell cytotoxicity mRNA expression serum folate 5-methyl-THF |
| title_short |
A Daily Dose of 5 mg Folic Acid for 90 Days Is Associated with Increased Serum Unmetabolized Folic Acid and Reduced Natural Killer Cell Cytotoxicity in Healthy Brazilian Adults |
| title_full |
A Daily Dose of 5 mg Folic Acid for 90 Days Is Associated with Increased Serum Unmetabolized Folic Acid and Reduced Natural Killer Cell Cytotoxicity in Healthy Brazilian Adults |
| title_fullStr |
A Daily Dose of 5 mg Folic Acid for 90 Days Is Associated with Increased Serum Unmetabolized Folic Acid and Reduced Natural Killer Cell Cytotoxicity in Healthy Brazilian Adults |
| title_full_unstemmed |
A Daily Dose of 5 mg Folic Acid for 90 Days Is Associated with Increased Serum Unmetabolized Folic Acid and Reduced Natural Killer Cell Cytotoxicity in Healthy Brazilian Adults |
| title_sort |
A Daily Dose of 5 mg Folic Acid for 90 Days Is Associated with Increased Serum Unmetabolized Folic Acid and Reduced Natural Killer Cell Cytotoxicity in Healthy Brazilian Adults |
| author |
Paniz, Clovis |
| author_facet |
Paniz, Clovis Bertinato, Juliano Felix Lucena, Maylla Rodrigues [UNIFESP] De Carli, Eduardo da Silva Amorim, Patricia Mendonca Gomes, Guilherme Wataru Palchetti, Cecilia Zanin Figueiredo, Maria Stella [UNIFESP] Pfeiffer, Christine M. Fazili, Zia Green, Ralph Guerra-Shinohara, Elvira Maria [UNIFESP] |
| author_role |
author |
| author2 |
Bertinato, Juliano Felix Lucena, Maylla Rodrigues [UNIFESP] De Carli, Eduardo da Silva Amorim, Patricia Mendonca Gomes, Guilherme Wataru Palchetti, Cecilia Zanin Figueiredo, Maria Stella [UNIFESP] Pfeiffer, Christine M. Fazili, Zia Green, Ralph Guerra-Shinohara, Elvira Maria [UNIFESP] |
| author2_role |
author author author author author author author author author author author |
| dc.contributor.author.fl_str_mv |
Paniz, Clovis Bertinato, Juliano Felix Lucena, Maylla Rodrigues [UNIFESP] De Carli, Eduardo da Silva Amorim, Patricia Mendonca Gomes, Guilherme Wataru Palchetti, Cecilia Zanin Figueiredo, Maria Stella [UNIFESP] Pfeiffer, Christine M. Fazili, Zia Green, Ralph Guerra-Shinohara, Elvira Maria [UNIFESP] |
| dc.subject.por.fl_str_mv |
folic acid supplementation unmetabolized folic acid NK cell cytotoxicity mRNA expression serum folate 5-methyl-THF |
| topic |
folic acid supplementation unmetabolized folic acid NK cell cytotoxicity mRNA expression serum folate 5-methyl-THF |
| description |
Background: The effects of high-dose folic acid (FA) supplementation in healthy individuals on blood folate concentrations and immune response are unknown. Objective: The aim of the study was to evaluate the effects of daily consumption of a tablet containing 5 mg FA on serum folate |
| publishDate |
2017 |
| dc.date.none.fl_str_mv |
2017 2019-08-19T11:48:31Z 2019-08-19T11:48:31Z |
| dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
| dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
| format |
article |
| status_str |
publishedVersion |
| dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.3945/jn.117.247445 Journal Of Nutrition. Bethesda, v. 147, n. 9, p. 1684-1692, 2017. 10.3945/jn.117.247445 0022-3166 http://repositorio.unifesp.br/handle/11600/51271 WOS:000411807800012 |
| url |
http://dx.doi.org/10.3945/jn.117.247445 http://repositorio.unifesp.br/handle/11600/51271 |
| identifier_str_mv |
Journal Of Nutrition. Bethesda, v. 147, n. 9, p. 1684-1692, 2017. 10.3945/jn.117.247445 0022-3166 WOS:000411807800012 |
| dc.language.iso.fl_str_mv |
eng |
| language |
eng |
| dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
| eu_rights_str_mv |
openAccess |
| dc.format.none.fl_str_mv |
1684-1692 |
| dc.publisher.none.fl_str_mv |
Amer Soc Nutrition-Asn |
| publisher.none.fl_str_mv |
Amer Soc Nutrition-Asn |
| dc.source.none.fl_str_mv |
reponame:Repositório Institucional da UNIFESP instname:Universidade Federal de São Paulo (UNIFESP) instacron:UNIFESP |
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Universidade Federal de São Paulo (UNIFESP) |
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UNIFESP |
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UNIFESP |
| reponame_str |
Repositório Institucional da UNIFESP |
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Repositório Institucional da UNIFESP |
| repository.name.fl_str_mv |
Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP) |
| repository.mail.fl_str_mv |
biblioteca.csp@unifesp.br |
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1814268392851374080 |