Evaluation of bacterial diversity recovered from petroleum samples using different physical matrices
Autor(a) principal: | |
---|---|
Data de Publicação: | 2016 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNIFESP |
Texto Completo: | https://repositorio.unifesp.br/handle/11600/57647 http://dx.doi.org/10.1016/j.bjm.2016.04.004 |
Resumo: | Unraveling the microbial diversity and its complexity in petroleum reservoir environments has been a challenge throughout the years. Despite the techniques developed in order to improve methodologies involving DNA extraction from crude oil, microbial enrichments using different culture conditions can be applied as a way to increase the recovery of DNA from environments with low cellular density for further microbiological analyses. This work aimed at the evaluation of different matrices (arenite, shale and polyurethane foam) as support materials for microbial growth and biofilm formation in enrichments using a biodegraded petroleum sample as inoculum in sulfate reducing condition. Subsequent microbial diversity characterization was carried out using Scanning Electronic Microscopy (SEM), Denaturing Gradient Gel Electrophoresis (DGGE) and 16S rRNA gene libraries in order to compare the microbial biomass yield, DNA recovery efficiency and diversity among the enrichments. The DNA from microbial communities in petroleum enrichments was purified according to a protocol established in this work and used for 16S rRNA amplification with bacterial generic primers. The PCR products were cloned, and positive clones were screened by Amplified Ribosomal DNA Restriction Analysis (ARDRA). Sequencing and phylogenetic analyses revealed that the bacterial community was mostly represented by members of the genera Petrotoga, Bacillus, Pseudomonas, Geobacillus and Rahnella. The use of different support materials in the enrichments yielded an increase in microbial biomass and biofilm formation, indicating that these materials may be employed for efficient biomass recovery from petroleum reservoir samples. Nonetheless, the most diverse microbiota were recovered from the biodegraded petroleum sample using polyurethane foam cubes as support material. (C) 2016 Sociedade Brasileira de Microbiologia. Published by Elsevier Editora Ltda. This is an open access article under the CC BY-NC-ND license |
id |
UFSP_f045bbb6a41a23ae539580f4c694c4ef |
---|---|
oai_identifier_str |
oai:repositorio.unifesp.br:11600/57647 |
network_acronym_str |
UFSP |
network_name_str |
Repositório Institucional da UNIFESP |
repository_id_str |
3465 |
spelling |
Dellagnezze, Bruna Martinsde Vasconcellos, Suzan Pantarotode Melo, Itamar Soaresdos Santos Neto, Eugenio Vazde Oliveira, Valeria Maia2020-08-14T13:44:24Z2020-08-14T13:44:24Z2016Brazilian Journal Of Microbiology. Sao Paulo, v. 47, n. 3, p. 712-723, 2016.1517-8382https://repositorio.unifesp.br/handle/11600/57647http://dx.doi.org/10.1016/j.bjm.2016.04.004WOS000381101200026.pdfS1517-8382201600030071210.1016/j.bjm.2016.04.004WOS:000381101200026Unraveling the microbial diversity and its complexity in petroleum reservoir environments has been a challenge throughout the years. Despite the techniques developed in order to improve methodologies involving DNA extraction from crude oil, microbial enrichments using different culture conditions can be applied as a way to increase the recovery of DNA from environments with low cellular density for further microbiological analyses. This work aimed at the evaluation of different matrices (arenite, shale and polyurethane foam) as support materials for microbial growth and biofilm formation in enrichments using a biodegraded petroleum sample as inoculum in sulfate reducing condition. Subsequent microbial diversity characterization was carried out using Scanning Electronic Microscopy (SEM), Denaturing Gradient Gel Electrophoresis (DGGE) and 16S rRNA gene libraries in order to compare the microbial biomass yield, DNA recovery efficiency and diversity among the enrichments. The DNA from microbial communities in petroleum enrichments was purified according to a protocol established in this work and used for 16S rRNA amplification with bacterial generic primers. The PCR products were cloned, and positive clones were screened by Amplified Ribosomal DNA Restriction Analysis (ARDRA). Sequencing and phylogenetic analyses revealed that the bacterial community was mostly represented by members of the genera Petrotoga, Bacillus, Pseudomonas, Geobacillus and Rahnella. The use of different support materials in the enrichments yielded an increase in microbial biomass and biofilm formation, indicating that these materials may be employed for efficient biomass recovery from petroleum reservoir samples. Nonetheless, the most diverse microbiota were recovered from the biodegraded petroleum sample using polyurethane foam cubes as support material. (C) 2016 Sociedade Brasileira de Microbiologia. Published by Elsevier Editora Ltda. This is an open access article under the CC BY-NC-ND licenseUniv Campinas UNICAMP, Res Ctr Chem, Div Microbial Resources, Biol,Agr CPQBA, Campinas, SP, BrazilFed Univ Sao Paulo UNIFESP, Dept Biol Sci, Diadema, SP, BrazilEMBRAPA, Natl Ctr Res Monitoring & Evaluating Environm Imp, Jaguariuna, SP, BrazilPETROBRAS R&D Ctr, Rio De Janeiro, RJ, BrazilFed Univ Sao Paulo UNIFESP, Dept Biol Sci, Diadema, SP, BrazilWeb of Science712-723engSoc Brasileira MicrobiologiaBrazilian Journal Of MicrobiologyBacterial diversityMicrobial enrichmentPetroleum reservoirPhysical support16S rRNAEvaluation of bacterial diversity recovered from petroleum samples using different physical matricesinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleSao Paulo473info:eu-repo/semantics/openAccessreponame:Repositório Institucional da UNIFESPinstname:Universidade Federal de São Paulo (UNIFESP)instacron:UNIFESPORIGINALWOS000381101200026.pdfapplication/pdf2748541${dspace.ui.url}/bitstream/11600/57647/1/WOS000381101200026.pdfc97205f8256ba989222dda902556d91cMD51open accessTEXTWOS000381101200026.pdf.txtWOS000381101200026.pdf.txtExtracted texttext/plain54562${dspace.ui.url}/bitstream/11600/57647/8/WOS000381101200026.pdf.txt0c150118313fbaf434424b6d7c6f7d56MD58open accessTHUMBNAILWOS000381101200026.pdf.jpgWOS000381101200026.pdf.jpgIM Thumbnailimage/jpeg6836${dspace.ui.url}/bitstream/11600/57647/10/WOS000381101200026.pdf.jpg61e25d4fc5e1804eafaebbc6f530ee94MD510open access11600/576472023-06-05 19:22:43.24open accessoai:repositorio.unifesp.br:11600/57647Repositório InstitucionalPUBhttp://www.repositorio.unifesp.br/oai/requestopendoar:34652023-06-05T22:22:43Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)false |
dc.title.en.fl_str_mv |
Evaluation of bacterial diversity recovered from petroleum samples using different physical matrices |
title |
Evaluation of bacterial diversity recovered from petroleum samples using different physical matrices |
spellingShingle |
Evaluation of bacterial diversity recovered from petroleum samples using different physical matrices Dellagnezze, Bruna Martins Bacterial diversity Microbial enrichment Petroleum reservoir Physical support 16S rRNA |
title_short |
Evaluation of bacterial diversity recovered from petroleum samples using different physical matrices |
title_full |
Evaluation of bacterial diversity recovered from petroleum samples using different physical matrices |
title_fullStr |
Evaluation of bacterial diversity recovered from petroleum samples using different physical matrices |
title_full_unstemmed |
Evaluation of bacterial diversity recovered from petroleum samples using different physical matrices |
title_sort |
Evaluation of bacterial diversity recovered from petroleum samples using different physical matrices |
author |
Dellagnezze, Bruna Martins |
author_facet |
Dellagnezze, Bruna Martins de Vasconcellos, Suzan Pantaroto de Melo, Itamar Soares dos Santos Neto, Eugenio Vaz de Oliveira, Valeria Maia |
author_role |
author |
author2 |
de Vasconcellos, Suzan Pantaroto de Melo, Itamar Soares dos Santos Neto, Eugenio Vaz de Oliveira, Valeria Maia |
author2_role |
author author author author |
dc.contributor.author.fl_str_mv |
Dellagnezze, Bruna Martins de Vasconcellos, Suzan Pantaroto de Melo, Itamar Soares dos Santos Neto, Eugenio Vaz de Oliveira, Valeria Maia |
dc.subject.eng.fl_str_mv |
Bacterial diversity Microbial enrichment Petroleum reservoir Physical support 16S rRNA |
topic |
Bacterial diversity Microbial enrichment Petroleum reservoir Physical support 16S rRNA |
description |
Unraveling the microbial diversity and its complexity in petroleum reservoir environments has been a challenge throughout the years. Despite the techniques developed in order to improve methodologies involving DNA extraction from crude oil, microbial enrichments using different culture conditions can be applied as a way to increase the recovery of DNA from environments with low cellular density for further microbiological analyses. This work aimed at the evaluation of different matrices (arenite, shale and polyurethane foam) as support materials for microbial growth and biofilm formation in enrichments using a biodegraded petroleum sample as inoculum in sulfate reducing condition. Subsequent microbial diversity characterization was carried out using Scanning Electronic Microscopy (SEM), Denaturing Gradient Gel Electrophoresis (DGGE) and 16S rRNA gene libraries in order to compare the microbial biomass yield, DNA recovery efficiency and diversity among the enrichments. The DNA from microbial communities in petroleum enrichments was purified according to a protocol established in this work and used for 16S rRNA amplification with bacterial generic primers. The PCR products were cloned, and positive clones were screened by Amplified Ribosomal DNA Restriction Analysis (ARDRA). Sequencing and phylogenetic analyses revealed that the bacterial community was mostly represented by members of the genera Petrotoga, Bacillus, Pseudomonas, Geobacillus and Rahnella. The use of different support materials in the enrichments yielded an increase in microbial biomass and biofilm formation, indicating that these materials may be employed for efficient biomass recovery from petroleum reservoir samples. Nonetheless, the most diverse microbiota were recovered from the biodegraded petroleum sample using polyurethane foam cubes as support material. (C) 2016 Sociedade Brasileira de Microbiologia. Published by Elsevier Editora Ltda. This is an open access article under the CC BY-NC-ND license |
publishDate |
2016 |
dc.date.issued.fl_str_mv |
2016 |
dc.date.accessioned.fl_str_mv |
2020-08-14T13:44:24Z |
dc.date.available.fl_str_mv |
2020-08-14T13:44:24Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
Brazilian Journal Of Microbiology. Sao Paulo, v. 47, n. 3, p. 712-723, 2016. |
dc.identifier.uri.fl_str_mv |
https://repositorio.unifesp.br/handle/11600/57647 http://dx.doi.org/10.1016/j.bjm.2016.04.004 |
dc.identifier.issn.none.fl_str_mv |
1517-8382 |
dc.identifier.file.none.fl_str_mv |
WOS000381101200026.pdf |
dc.identifier.scielo.none.fl_str_mv |
S1517-83822016000300712 |
dc.identifier.doi.none.fl_str_mv |
10.1016/j.bjm.2016.04.004 |
dc.identifier.wos.none.fl_str_mv |
WOS:000381101200026 |
identifier_str_mv |
Brazilian Journal Of Microbiology. Sao Paulo, v. 47, n. 3, p. 712-723, 2016. 1517-8382 WOS000381101200026.pdf S1517-83822016000300712 10.1016/j.bjm.2016.04.004 WOS:000381101200026 |
url |
https://repositorio.unifesp.br/handle/11600/57647 http://dx.doi.org/10.1016/j.bjm.2016.04.004 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.ispartof.none.fl_str_mv |
Brazilian Journal Of Microbiology |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
712-723 |
dc.coverage.none.fl_str_mv |
Sao Paulo |
dc.publisher.none.fl_str_mv |
Soc Brasileira Microbiologia |
publisher.none.fl_str_mv |
Soc Brasileira Microbiologia |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da UNIFESP instname:Universidade Federal de São Paulo (UNIFESP) instacron:UNIFESP |
instname_str |
Universidade Federal de São Paulo (UNIFESP) |
instacron_str |
UNIFESP |
institution |
UNIFESP |
reponame_str |
Repositório Institucional da UNIFESP |
collection |
Repositório Institucional da UNIFESP |
bitstream.url.fl_str_mv |
${dspace.ui.url}/bitstream/11600/57647/1/WOS000381101200026.pdf ${dspace.ui.url}/bitstream/11600/57647/8/WOS000381101200026.pdf.txt ${dspace.ui.url}/bitstream/11600/57647/10/WOS000381101200026.pdf.jpg |
bitstream.checksum.fl_str_mv |
c97205f8256ba989222dda902556d91c 0c150118313fbaf434424b6d7c6f7d56 61e25d4fc5e1804eafaebbc6f530ee94 |
bitstream.checksumAlgorithm.fl_str_mv |
MD5 MD5 MD5 |
repository.name.fl_str_mv |
Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP) |
repository.mail.fl_str_mv |
|
_version_ |
1802764163728015360 |