Amazonian waters harbour an ancient freshwater Ceratomyxa lineage (Cnidaria: Myxosporea)
Autor(a) principal: | |
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Data de Publicação: | 2017 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNIFESP |
Texto Completo: | https://repositorio.unifesp.br/handle/11600/54698 http://dx.doi.org/10.1016/j.actatropica.2017.02.006 |
Resumo: | A new species of Ceratomyxa parasitizing the gall bladder of Cichla monoculus, an endemic cichlid fish from the Amazon basin in Brazil, is described using morphological and molecular data. In the bile, both immature and mature myxospores were found floating freely or inside elongated plasmodia: length 304 (196-402) mu m and width 35.7 (18.3-55.1) mu m. Mature spores were elongated and only slightly crescent-shaped in frontal view with a prominent sutural line between two valve cells, which had rounded ends. Measurements of formalin-fixed myxospores: length 6.3 +/- 0.6 (5.1-7.5) mu m, thickness 41.2 +/- 2.9 (37.1-47.6) mu m, posterior angle 147 degrees. Lateral projections slightly asymmetric, with lengths 19.3 +/- 1.4 mu m and 20.5 +/- 1.3 mu m. Two ovoid, equal size polar capsules, length 2.6 +/- 0.3 (2-3.3) mu m, width 2.5 +/- 0.4 (1.8-3.7) mu m, located adjacent to the suture and containing polar filaments with 3-4 turns. The small subunit ribosomal DNA sequence of 1605 nt was no more than 97% similar to any other sequence in GenBank, and together with the host, locality and morphometric data, supports diagnosis of the parasite as a new species, Ceratomyxa brasiliensis n. sp. Maximum parsimony and maximum likelihood analyses showed that C brasiliensis n. sp. clustered within the marine Ceratomyxa Glade, but was in a basally divergent lineage with two other freshwater species from the Amazon basin. Our results are consistent with previous studies that show Ceratomyxa species can cluster according to both geography and host ecotype, and that the few known freshwater species diverged from marine cousins relatively early in evolution of the genus, possibly driven by marine incursions into riverine environments. (C) 2017 Elsevier B.V. All rights reserved. |
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Zatti, Suellen A.Atkinson, Stephen D.Bartholomew, Jerri L.Maia, Antonio A. M.Adriano, Edson A. [UNIFESP]2020-07-17T14:02:14Z2020-07-17T14:02:14Z2017Acta Tropica. Amsterdam, v. 169, p. 100-106, 2017.0001-706Xhttps://repositorio.unifesp.br/handle/11600/54698http://dx.doi.org/10.1016/j.actatropica.2017.02.00610.1016/j.actatropica.2017.02.006WOS:000396954000013A new species of Ceratomyxa parasitizing the gall bladder of Cichla monoculus, an endemic cichlid fish from the Amazon basin in Brazil, is described using morphological and molecular data. In the bile, both immature and mature myxospores were found floating freely or inside elongated plasmodia: length 304 (196-402) mu m and width 35.7 (18.3-55.1) mu m. Mature spores were elongated and only slightly crescent-shaped in frontal view with a prominent sutural line between two valve cells, which had rounded ends. Measurements of formalin-fixed myxospores: length 6.3 +/- 0.6 (5.1-7.5) mu m, thickness 41.2 +/- 2.9 (37.1-47.6) mu m, posterior angle 147 degrees. Lateral projections slightly asymmetric, with lengths 19.3 +/- 1.4 mu m and 20.5 +/- 1.3 mu m. Two ovoid, equal size polar capsules, length 2.6 +/- 0.3 (2-3.3) mu m, width 2.5 +/- 0.4 (1.8-3.7) mu m, located adjacent to the suture and containing polar filaments with 3-4 turns. The small subunit ribosomal DNA sequence of 1605 nt was no more than 97% similar to any other sequence in GenBank, and together with the host, locality and morphometric data, supports diagnosis of the parasite as a new species, Ceratomyxa brasiliensis n. sp. Maximum parsimony and maximum likelihood analyses showed that C brasiliensis n. sp. clustered within the marine Ceratomyxa Glade, but was in a basally divergent lineage with two other freshwater species from the Amazon basin. Our results are consistent with previous studies that show Ceratomyxa species can cluster according to both geography and host ecotype, and that the few known freshwater species diverged from marine cousins relatively early in evolution of the genus, possibly driven by marine incursions into riverine environments. (C) 2017 Elsevier B.V. All rights reserved.CAPES Foundation within the Ministry of Education, Brazil [BEX - 6729/2015-00]CAPESSao Paulo Research Foundation - FAPESP [2013/21374-6]Brazilian Fostering Agency CNPq [305630/2013-0]Univ Estadual Campinas, Dept Anim Biol, Inst Biol, Rua Monteiro Lobato 255, BR-13083862 Campinas, SP, BrazilOregon State Univ, Dept Microbiol, Corvallis, OR 97331 USAUniv Sao Paulo, Fac Anim Sci & Food Engn, Dept Vet Med, Ave Duque Caxias Norte 225, BR-13635900 Pirassununga, SP, BrazilUniv Fed Sao Paulo, Dept Ecol & Evolutionary Biol, Rua Prof Arthur Riedel 275, BR-09972270 Diadema, SP, BrazilUniv Fed Sao Paulo, Dept Ecol & Evolutionary Biol, Rua Prof Arthur Riedel 275, BR-09972270 Diadema, SP, BrazilWeb of Science100-106engElsevier Science BvActa TropicaMyxozoaCeratomyxassrDNA sequencingFreshwater environmentAmazonAmazonian waters harbour an ancient freshwater Ceratomyxa lineage (Cnidaria: Myxosporea)info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleAmsterdam169info:eu-repo/semantics/openAccessreponame:Repositório Institucional da UNIFESPinstname:Universidade Federal de São Paulo (UNIFESP)instacron:UNIFESP11600/546982021-09-28 16:10:24.49metadata only accessoai:repositorio.unifesp.br:11600/54698Repositório InstitucionalPUBhttp://www.repositorio.unifesp.br/oai/requestopendoar:34652023-05-25T12:09:10.459161Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)false |
dc.title.en.fl_str_mv |
Amazonian waters harbour an ancient freshwater Ceratomyxa lineage (Cnidaria: Myxosporea) |
title |
Amazonian waters harbour an ancient freshwater Ceratomyxa lineage (Cnidaria: Myxosporea) |
spellingShingle |
Amazonian waters harbour an ancient freshwater Ceratomyxa lineage (Cnidaria: Myxosporea) Zatti, Suellen A. Myxozoa Ceratomyxa ssrDNA sequencing Freshwater environment Amazon |
title_short |
Amazonian waters harbour an ancient freshwater Ceratomyxa lineage (Cnidaria: Myxosporea) |
title_full |
Amazonian waters harbour an ancient freshwater Ceratomyxa lineage (Cnidaria: Myxosporea) |
title_fullStr |
Amazonian waters harbour an ancient freshwater Ceratomyxa lineage (Cnidaria: Myxosporea) |
title_full_unstemmed |
Amazonian waters harbour an ancient freshwater Ceratomyxa lineage (Cnidaria: Myxosporea) |
title_sort |
Amazonian waters harbour an ancient freshwater Ceratomyxa lineage (Cnidaria: Myxosporea) |
author |
Zatti, Suellen A. |
author_facet |
Zatti, Suellen A. Atkinson, Stephen D. Bartholomew, Jerri L. Maia, Antonio A. M. Adriano, Edson A. [UNIFESP] |
author_role |
author |
author2 |
Atkinson, Stephen D. Bartholomew, Jerri L. Maia, Antonio A. M. Adriano, Edson A. [UNIFESP] |
author2_role |
author author author author |
dc.contributor.author.fl_str_mv |
Zatti, Suellen A. Atkinson, Stephen D. Bartholomew, Jerri L. Maia, Antonio A. M. Adriano, Edson A. [UNIFESP] |
dc.subject.eng.fl_str_mv |
Myxozoa Ceratomyxa ssrDNA sequencing Freshwater environment Amazon |
topic |
Myxozoa Ceratomyxa ssrDNA sequencing Freshwater environment Amazon |
description |
A new species of Ceratomyxa parasitizing the gall bladder of Cichla monoculus, an endemic cichlid fish from the Amazon basin in Brazil, is described using morphological and molecular data. In the bile, both immature and mature myxospores were found floating freely or inside elongated plasmodia: length 304 (196-402) mu m and width 35.7 (18.3-55.1) mu m. Mature spores were elongated and only slightly crescent-shaped in frontal view with a prominent sutural line between two valve cells, which had rounded ends. Measurements of formalin-fixed myxospores: length 6.3 +/- 0.6 (5.1-7.5) mu m, thickness 41.2 +/- 2.9 (37.1-47.6) mu m, posterior angle 147 degrees. Lateral projections slightly asymmetric, with lengths 19.3 +/- 1.4 mu m and 20.5 +/- 1.3 mu m. Two ovoid, equal size polar capsules, length 2.6 +/- 0.3 (2-3.3) mu m, width 2.5 +/- 0.4 (1.8-3.7) mu m, located adjacent to the suture and containing polar filaments with 3-4 turns. The small subunit ribosomal DNA sequence of 1605 nt was no more than 97% similar to any other sequence in GenBank, and together with the host, locality and morphometric data, supports diagnosis of the parasite as a new species, Ceratomyxa brasiliensis n. sp. Maximum parsimony and maximum likelihood analyses showed that C brasiliensis n. sp. clustered within the marine Ceratomyxa Glade, but was in a basally divergent lineage with two other freshwater species from the Amazon basin. Our results are consistent with previous studies that show Ceratomyxa species can cluster according to both geography and host ecotype, and that the few known freshwater species diverged from marine cousins relatively early in evolution of the genus, possibly driven by marine incursions into riverine environments. (C) 2017 Elsevier B.V. All rights reserved. |
publishDate |
2017 |
dc.date.issued.fl_str_mv |
2017 |
dc.date.accessioned.fl_str_mv |
2020-07-17T14:02:14Z |
dc.date.available.fl_str_mv |
2020-07-17T14:02:14Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
Acta Tropica. Amsterdam, v. 169, p. 100-106, 2017. |
dc.identifier.uri.fl_str_mv |
https://repositorio.unifesp.br/handle/11600/54698 http://dx.doi.org/10.1016/j.actatropica.2017.02.006 |
dc.identifier.issn.none.fl_str_mv |
0001-706X |
dc.identifier.doi.none.fl_str_mv |
10.1016/j.actatropica.2017.02.006 |
dc.identifier.wos.none.fl_str_mv |
WOS:000396954000013 |
identifier_str_mv |
Acta Tropica. Amsterdam, v. 169, p. 100-106, 2017. 0001-706X 10.1016/j.actatropica.2017.02.006 WOS:000396954000013 |
url |
https://repositorio.unifesp.br/handle/11600/54698 http://dx.doi.org/10.1016/j.actatropica.2017.02.006 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.ispartof.none.fl_str_mv |
Acta Tropica |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
100-106 |
dc.coverage.none.fl_str_mv |
Amsterdam |
dc.publisher.none.fl_str_mv |
Elsevier Science Bv |
publisher.none.fl_str_mv |
Elsevier Science Bv |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da UNIFESP instname:Universidade Federal de São Paulo (UNIFESP) instacron:UNIFESP |
instname_str |
Universidade Federal de São Paulo (UNIFESP) |
instacron_str |
UNIFESP |
institution |
UNIFESP |
reponame_str |
Repositório Institucional da UNIFESP |
collection |
Repositório Institucional da UNIFESP |
repository.name.fl_str_mv |
Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP) |
repository.mail.fl_str_mv |
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1783460255849512960 |