Mapping of B-cell epitopes in a antigen expressed Trypanosoma cruzi immunodominant in natural infections
Autor(a) principal: | |
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Data de Publicação: | 2005 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNIFESP |
Texto Completo: | http://repositorio.unifesp.br/handle/11600/28127 http://dx.doi.org/10.1128/CDLI.12.2.329-333.2005 |
Resumo: | Tc40 is an immunodominant antigen present in natural Trypanosoma cruzi infections. This immunogen was thoroughly mapped by using overlapping amino acid sequences identified by gene cloning and chemical peptide synthesis. To map continuous epitopes of the Tc40 antigen, an epitope expression library was constructed and screened with sera from human chagasic patients. A major, linear B-cell epitope spanning residues 403 to 426 (PAKAAAPPAA) was identified in the central domain of Tc40. A synthetic peptide spanning this region reacted strongly with 89.8% of the serum samples from T. cruzi-infected individuals. This indicates that the main antigenic site is defined by the linear sequence of the peptide rather than a conformation-dependent structure. the major B-cell epitope of Tc40 shares a high degree of sequence identity with T. cruzi ribosomal and RNA binding proteins, suggesting the existence of cross-reactivity among these molecules. |
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Lesenechal, MyleneBecquart, LaurenceLacoux, XavierLadaviere, LaurentBaida, Renata Cristina Pardos [UNIFESP]Paranhos-Baccala, GlauciaSilveira, Jose Franco da [UNIFESP]Universidade Federal de São Paulo (UNIFESP)CNRS bioMerieuxUniv Lyon 12016-01-24T12:37:37Z2016-01-24T12:37:37Z2005-02-01Clinical and Diagnostic Laboratory Immunology. Washington: Amer Soc Microbiology, v. 12, n. 2, p. 329-333, 2005.1071-412Xhttp://repositorio.unifesp.br/handle/11600/28127http://dx.doi.org/10.1128/CDLI.12.2.329-333.2005WOS000231196400014.pdf10.1128/CDLI.12.2.329-333.2005WOS:000231196400014Tc40 is an immunodominant antigen present in natural Trypanosoma cruzi infections. This immunogen was thoroughly mapped by using overlapping amino acid sequences identified by gene cloning and chemical peptide synthesis. To map continuous epitopes of the Tc40 antigen, an epitope expression library was constructed and screened with sera from human chagasic patients. A major, linear B-cell epitope spanning residues 403 to 426 (PAKAAAPPAA) was identified in the central domain of Tc40. A synthetic peptide spanning this region reacted strongly with 89.8% of the serum samples from T. cruzi-infected individuals. This indicates that the main antigenic site is defined by the linear sequence of the peptide rather than a conformation-dependent structure. the major B-cell epitope of Tc40 shares a high degree of sequence identity with T. cruzi ribosomal and RNA binding proteins, suggesting the existence of cross-reactivity among these molecules.Universidade Federal de São Paulo, Dept Microbiol Immunol & Parasitol, Escola Paulista Med, São Paulo, BrazilCNRS bioMerieux, UMR 2714, IFR 128 BioSci Lyon Gerland, CERVI 21, Lyon, FranceUniv Lyon 1, CNRS, UMR 5086, Lab Bioinformat & RMN Struct,IFR 128 BioSci Lyon, F-69365 Lyon, FranceUniversidade Federal de São Paulo, Dept Microbiol Immunol & Parasitol, Escola Paulista Med, São Paulo, BrazilWeb of Science329-333engAmer Soc MicrobiologyClinical and Diagnostic Laboratory ImmunologyMapping of B-cell epitopes in a antigen expressed Trypanosoma cruzi immunodominant in natural infectionsinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UNIFESPinstname:Universidade Federal de São Paulo (UNIFESP)instacron:UNIFESPORIGINALWOS000231196400014.pdfapplication/pdf149975${dspace.ui.url}/bitstream/11600/28127/1/WOS000231196400014.pdfe9199cff58dbc21e77d4751b9f2df63aMD51open accessTEXTWOS000231196400014.pdf.txtWOS000231196400014.pdf.txtExtracted texttext/plain27946${dspace.ui.url}/bitstream/11600/28127/2/WOS000231196400014.pdf.txt923841cadd3cf00f54cb5dc8d002fc97MD52open access11600/281272023-01-27 22:45:17.662open accessoai:repositorio.unifesp.br:11600/28127Repositório InstitucionalPUBhttp://www.repositorio.unifesp.br/oai/requestopendoar:34652023-01-28T01:45:17Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)false |
dc.title.en.fl_str_mv |
Mapping of B-cell epitopes in a antigen expressed Trypanosoma cruzi immunodominant in natural infections |
title |
Mapping of B-cell epitopes in a antigen expressed Trypanosoma cruzi immunodominant in natural infections |
spellingShingle |
Mapping of B-cell epitopes in a antigen expressed Trypanosoma cruzi immunodominant in natural infections Lesenechal, Mylene |
title_short |
Mapping of B-cell epitopes in a antigen expressed Trypanosoma cruzi immunodominant in natural infections |
title_full |
Mapping of B-cell epitopes in a antigen expressed Trypanosoma cruzi immunodominant in natural infections |
title_fullStr |
Mapping of B-cell epitopes in a antigen expressed Trypanosoma cruzi immunodominant in natural infections |
title_full_unstemmed |
Mapping of B-cell epitopes in a antigen expressed Trypanosoma cruzi immunodominant in natural infections |
title_sort |
Mapping of B-cell epitopes in a antigen expressed Trypanosoma cruzi immunodominant in natural infections |
author |
Lesenechal, Mylene |
author_facet |
Lesenechal, Mylene Becquart, Laurence Lacoux, Xavier Ladaviere, Laurent Baida, Renata Cristina Pardos [UNIFESP] Paranhos-Baccala, Glaucia Silveira, Jose Franco da [UNIFESP] |
author_role |
author |
author2 |
Becquart, Laurence Lacoux, Xavier Ladaviere, Laurent Baida, Renata Cristina Pardos [UNIFESP] Paranhos-Baccala, Glaucia Silveira, Jose Franco da [UNIFESP] |
author2_role |
author author author author author author |
dc.contributor.institution.none.fl_str_mv |
Universidade Federal de São Paulo (UNIFESP) CNRS bioMerieux Univ Lyon 1 |
dc.contributor.author.fl_str_mv |
Lesenechal, Mylene Becquart, Laurence Lacoux, Xavier Ladaviere, Laurent Baida, Renata Cristina Pardos [UNIFESP] Paranhos-Baccala, Glaucia Silveira, Jose Franco da [UNIFESP] |
description |
Tc40 is an immunodominant antigen present in natural Trypanosoma cruzi infections. This immunogen was thoroughly mapped by using overlapping amino acid sequences identified by gene cloning and chemical peptide synthesis. To map continuous epitopes of the Tc40 antigen, an epitope expression library was constructed and screened with sera from human chagasic patients. A major, linear B-cell epitope spanning residues 403 to 426 (PAKAAAPPAA) was identified in the central domain of Tc40. A synthetic peptide spanning this region reacted strongly with 89.8% of the serum samples from T. cruzi-infected individuals. This indicates that the main antigenic site is defined by the linear sequence of the peptide rather than a conformation-dependent structure. the major B-cell epitope of Tc40 shares a high degree of sequence identity with T. cruzi ribosomal and RNA binding proteins, suggesting the existence of cross-reactivity among these molecules. |
publishDate |
2005 |
dc.date.issued.fl_str_mv |
2005-02-01 |
dc.date.accessioned.fl_str_mv |
2016-01-24T12:37:37Z |
dc.date.available.fl_str_mv |
2016-01-24T12:37:37Z |
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info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
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article |
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publishedVersion |
dc.identifier.citation.fl_str_mv |
Clinical and Diagnostic Laboratory Immunology. Washington: Amer Soc Microbiology, v. 12, n. 2, p. 329-333, 2005. |
dc.identifier.uri.fl_str_mv |
http://repositorio.unifesp.br/handle/11600/28127 http://dx.doi.org/10.1128/CDLI.12.2.329-333.2005 |
dc.identifier.issn.none.fl_str_mv |
1071-412X |
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WOS000231196400014.pdf |
dc.identifier.doi.none.fl_str_mv |
10.1128/CDLI.12.2.329-333.2005 |
dc.identifier.wos.none.fl_str_mv |
WOS:000231196400014 |
identifier_str_mv |
Clinical and Diagnostic Laboratory Immunology. Washington: Amer Soc Microbiology, v. 12, n. 2, p. 329-333, 2005. 1071-412X WOS000231196400014.pdf 10.1128/CDLI.12.2.329-333.2005 WOS:000231196400014 |
url |
http://repositorio.unifesp.br/handle/11600/28127 http://dx.doi.org/10.1128/CDLI.12.2.329-333.2005 |
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Clinical and Diagnostic Laboratory Immunology |
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329-333 |
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Amer Soc Microbiology |
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Amer Soc Microbiology |
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