A bovine protocol for training professionals in preimplantation genetics diagnosis using polymerase chain reactions
Autor(a) principal: | |
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Data de Publicação: | 2005 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNIFESP |
Texto Completo: | http://repositorio.unifesp.br/handle/11600/28474 http://dx.doi.org/10.1016/j.fertnstert.2005.02.051 |
Resumo: | Objective: To develop a bovine protocol for training in preimplantation genetic diagnosis (PGD) using PCR.Design: Randomized study.Setting: Human reproduction PCR laboratory.Patient(s): Cow ovaries obtained from slaughterhouses.Intervention(s): the ovaries were punctured and the oocytes were matured and submitted to in vitro fertilization. On the third day after fertilization, the embryos were biopsied and 1-2 blastomeres removed. A blastomere and the rest of the embryo were submitted to PCR for sex determination.Main Outcome Measure(S): Establishment of a possible training protocol.Result(s): A total of 50 embryos and 50 biopsied blastomeres were submitted to DNA amplification for sexing. of the 50 embryos, 41 (82%) achieved successful DNA amplification and 9 (18%) did not. of the 50 biopsies, 31 (62%) amplified and 19 (38%) did not. in 27 (65.9%) of the 41 embryos with DNA amplification, sex was identified as female and in 14 (34.1%) as male. in 40 cases (80%) amplification and sex determination were in both embryos and blastomeres. Sex was identical in all these cases.Conclusion(s): This training model seems to be useful in identifying mistakes and difficulties and improving the professional's performance in the various stages of preimplantation genetic diagnosis. |
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Almodin, Carlos Gilberto [UNIFESP]Moron, Antonio Fernandes [UNIFESP]Kulay Junior, Luiz [UNIFESP]Minguetti-Camara, Vânia CibeleMoraes, Andréa Cristina [UNIFESP]Torloni, Maria Regina [UNIFESP]Materbaby Reprod Humana & GenetUniversidade Federal de São Paulo (UNIFESP)2016-01-24T12:38:04Z2016-01-24T12:38:04Z2005-10-01Fertility and Sterility. New York: Elsevier B.V., v. 84, n. 4, p. 895-899, 2005.0015-0282http://repositorio.unifesp.br/handle/11600/28474http://dx.doi.org/10.1016/j.fertnstert.2005.02.05110.1016/j.fertnstert.2005.02.051WOS:000232542000013Objective: To develop a bovine protocol for training in preimplantation genetic diagnosis (PGD) using PCR.Design: Randomized study.Setting: Human reproduction PCR laboratory.Patient(s): Cow ovaries obtained from slaughterhouses.Intervention(s): the ovaries were punctured and the oocytes were matured and submitted to in vitro fertilization. On the third day after fertilization, the embryos were biopsied and 1-2 blastomeres removed. A blastomere and the rest of the embryo were submitted to PCR for sex determination.Main Outcome Measure(S): Establishment of a possible training protocol.Result(s): A total of 50 embryos and 50 biopsied blastomeres were submitted to DNA amplification for sexing. of the 50 embryos, 41 (82%) achieved successful DNA amplification and 9 (18%) did not. of the 50 biopsies, 31 (62%) amplified and 19 (38%) did not. in 27 (65.9%) of the 41 embryos with DNA amplification, sex was identified as female and in 14 (34.1%) as male. in 40 cases (80%) amplification and sex determination were in both embryos and blastomeres. Sex was identical in all these cases.Conclusion(s): This training model seems to be useful in identifying mistakes and difficulties and improving the professional's performance in the various stages of preimplantation genetic diagnosis.Materbaby Reprod Humana & Genet, Maringa, Parana, BrazilUniversidade Federal de São Paulo, Dept Obstet, São Paulo, BrazilUniversidade Federal de São Paulo, Dept Obstet, São Paulo, BrazilWeb of Science895-899engElsevier B.V.Fertility and Sterilityhttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policyinfo:eu-repo/semantics/openAccessPGDtrainingbovineembryosmodelA bovine protocol for training professionals in preimplantation genetics diagnosis using polymerase chain reactionsinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlereponame:Repositório Institucional da UNIFESPinstname:Universidade Federal de São Paulo (UNIFESP)instacron:UNIFESP11600/284742023-02-15 10:56:37.712metadata only accessoai:repositorio.unifesp.br:11600/28474Repositório InstitucionalPUBhttp://www.repositorio.unifesp.br/oai/requestopendoar:34652023-02-15T13:56:37Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)false |
dc.title.en.fl_str_mv |
A bovine protocol for training professionals in preimplantation genetics diagnosis using polymerase chain reactions |
title |
A bovine protocol for training professionals in preimplantation genetics diagnosis using polymerase chain reactions |
spellingShingle |
A bovine protocol for training professionals in preimplantation genetics diagnosis using polymerase chain reactions Almodin, Carlos Gilberto [UNIFESP] PGD training bovine embryos model |
title_short |
A bovine protocol for training professionals in preimplantation genetics diagnosis using polymerase chain reactions |
title_full |
A bovine protocol for training professionals in preimplantation genetics diagnosis using polymerase chain reactions |
title_fullStr |
A bovine protocol for training professionals in preimplantation genetics diagnosis using polymerase chain reactions |
title_full_unstemmed |
A bovine protocol for training professionals in preimplantation genetics diagnosis using polymerase chain reactions |
title_sort |
A bovine protocol for training professionals in preimplantation genetics diagnosis using polymerase chain reactions |
author |
Almodin, Carlos Gilberto [UNIFESP] |
author_facet |
Almodin, Carlos Gilberto [UNIFESP] Moron, Antonio Fernandes [UNIFESP] Kulay Junior, Luiz [UNIFESP] Minguetti-Camara, Vânia Cibele Moraes, Andréa Cristina [UNIFESP] Torloni, Maria Regina [UNIFESP] |
author_role |
author |
author2 |
Moron, Antonio Fernandes [UNIFESP] Kulay Junior, Luiz [UNIFESP] Minguetti-Camara, Vânia Cibele Moraes, Andréa Cristina [UNIFESP] Torloni, Maria Regina [UNIFESP] |
author2_role |
author author author author author |
dc.contributor.institution.none.fl_str_mv |
Materbaby Reprod Humana & Genet Universidade Federal de São Paulo (UNIFESP) |
dc.contributor.author.fl_str_mv |
Almodin, Carlos Gilberto [UNIFESP] Moron, Antonio Fernandes [UNIFESP] Kulay Junior, Luiz [UNIFESP] Minguetti-Camara, Vânia Cibele Moraes, Andréa Cristina [UNIFESP] Torloni, Maria Regina [UNIFESP] |
dc.subject.eng.fl_str_mv |
PGD training bovine embryos model |
topic |
PGD training bovine embryos model |
description |
Objective: To develop a bovine protocol for training in preimplantation genetic diagnosis (PGD) using PCR.Design: Randomized study.Setting: Human reproduction PCR laboratory.Patient(s): Cow ovaries obtained from slaughterhouses.Intervention(s): the ovaries were punctured and the oocytes were matured and submitted to in vitro fertilization. On the third day after fertilization, the embryos were biopsied and 1-2 blastomeres removed. A blastomere and the rest of the embryo were submitted to PCR for sex determination.Main Outcome Measure(S): Establishment of a possible training protocol.Result(s): A total of 50 embryos and 50 biopsied blastomeres were submitted to DNA amplification for sexing. of the 50 embryos, 41 (82%) achieved successful DNA amplification and 9 (18%) did not. of the 50 biopsies, 31 (62%) amplified and 19 (38%) did not. in 27 (65.9%) of the 41 embryos with DNA amplification, sex was identified as female and in 14 (34.1%) as male. in 40 cases (80%) amplification and sex determination were in both embryos and blastomeres. Sex was identical in all these cases.Conclusion(s): This training model seems to be useful in identifying mistakes and difficulties and improving the professional's performance in the various stages of preimplantation genetic diagnosis. |
publishDate |
2005 |
dc.date.issued.fl_str_mv |
2005-10-01 |
dc.date.accessioned.fl_str_mv |
2016-01-24T12:38:04Z |
dc.date.available.fl_str_mv |
2016-01-24T12:38:04Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
Fertility and Sterility. New York: Elsevier B.V., v. 84, n. 4, p. 895-899, 2005. |
dc.identifier.uri.fl_str_mv |
http://repositorio.unifesp.br/handle/11600/28474 http://dx.doi.org/10.1016/j.fertnstert.2005.02.051 |
dc.identifier.issn.none.fl_str_mv |
0015-0282 |
dc.identifier.doi.none.fl_str_mv |
10.1016/j.fertnstert.2005.02.051 |
dc.identifier.wos.none.fl_str_mv |
WOS:000232542000013 |
identifier_str_mv |
Fertility and Sterility. New York: Elsevier B.V., v. 84, n. 4, p. 895-899, 2005. 0015-0282 10.1016/j.fertnstert.2005.02.051 WOS:000232542000013 |
url |
http://repositorio.unifesp.br/handle/11600/28474 http://dx.doi.org/10.1016/j.fertnstert.2005.02.051 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.ispartof.none.fl_str_mv |
Fertility and Sterility |
dc.rights.driver.fl_str_mv |
http://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
http://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
895-899 |
dc.publisher.none.fl_str_mv |
Elsevier B.V. |
publisher.none.fl_str_mv |
Elsevier B.V. |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da UNIFESP instname:Universidade Federal de São Paulo (UNIFESP) instacron:UNIFESP |
instname_str |
Universidade Federal de São Paulo (UNIFESP) |
instacron_str |
UNIFESP |
institution |
UNIFESP |
reponame_str |
Repositório Institucional da UNIFESP |
collection |
Repositório Institucional da UNIFESP |
repository.name.fl_str_mv |
Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP) |
repository.mail.fl_str_mv |
|
_version_ |
1802764195629891584 |