Serodiagnosis of leprosy and follow-up of household contacts using a commercial rapid test containing ND-O/LID-1 antigens
Autor(a) principal: | |
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Data de Publicação: | 2017 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNIFESP |
Texto Completo: | http://dx.doi.org/0305-7518/17/064053+10 https://repositorio.unifesp.br/handle/11600/53682 |
Resumo: | Introduction: Early diagnosis of leprosy, including the diagnosis of sub-clinical disease in contacts of known cases, would be a major advance. The signs of early leprosy are often difficult to assess and a reliable diagnostic test could play an important role in identifying cases and thus reducing transmission. Methods: Subjects were recruited at Centro de Saude Jardim Guanabara, Rondonopolis, Mato Grosso State, Brazil. Sera were obtained from 174 recently diagnosed leprosy patients, 409 household contacts, 53 endemic controls and 12 patients with active TB. 186 of the household contacts were re-tested on subsequent visits. Both the NDO-LID rapid test (Orange Life, Rio de Janeiro, Brazil) and an anti-PGL-1 ELISA test were used on all samples for comparison. Results: LID-1 was positive in 25 of 125 PB cases (20%), but in 41 of 49 MB cases (83.7%), while the anti-PGL-1 ELISA was positive in 8 (6.4%) and 35 (71.4%) cases, respectively. Specificity for LID-1 was 85% and for the anti-PGL-1 ELISA, 97%. Amongst household contacts, 9 of 409 (2.2%) were positive on the LID-1 test, and a further 51 (115%) were weakly positive |
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Silva, Eliane AparecidaRosa, Patricia SammarcoFernandes Belone, Andrea FariaBroch Coelho, Neusa MariaUra, SomeiTomimori, Jane [UNIFESP]2020-06-26T16:30:38Z2020-06-26T16:30:38Z2017http://dx.doi.org/0305-7518/17/064053+10Leprosy Review. Colchester, v. 88, n. 2, p. 174-183, 2017.0305-7518https://repositorio.unifesp.br/handle/11600/53682WOS000411656500003.pdf0305-7518/17/064053+10WOS:000411656500003Introduction: Early diagnosis of leprosy, including the diagnosis of sub-clinical disease in contacts of known cases, would be a major advance. The signs of early leprosy are often difficult to assess and a reliable diagnostic test could play an important role in identifying cases and thus reducing transmission. Methods: Subjects were recruited at Centro de Saude Jardim Guanabara, Rondonopolis, Mato Grosso State, Brazil. Sera were obtained from 174 recently diagnosed leprosy patients, 409 household contacts, 53 endemic controls and 12 patients with active TB. 186 of the household contacts were re-tested on subsequent visits. Both the NDO-LID rapid test (Orange Life, Rio de Janeiro, Brazil) and an anti-PGL-1 ELISA test were used on all samples for comparison. Results: LID-1 was positive in 25 of 125 PB cases (20%), but in 41 of 49 MB cases (83.7%), while the anti-PGL-1 ELISA was positive in 8 (6.4%) and 35 (71.4%) cases, respectively. Specificity for LID-1 was 85% and for the anti-PGL-1 ELISA, 97%. Amongst household contacts, 9 of 409 (2.2%) were positive on the LID-1 test, and a further 51 (115%) were weakly positivealthough 9 cases of leprosy were subsequently identified amongst these contacts, 6 had tested negative, 2 were weakly positive and only 1 had been positive. Conclusion: The ND-O-LID-1 assay can be easily performed by the addition of serum to the assay device, and thus can be applied in resource-poor settings. The test was found to be useful for the detection of multibacillary cases, which in the long term will help to reduce transmission of the disease. This is a specific test, but it is not very sensitive for early detection of leprosy in household contacts and paucibacillary forms.Inst Lauro Souza Lime, Bauru, SP, BrazilHlth Ctr Rondonopolis MT, Rondonopolis, BrazilFed Univ Sao Paulo Unifesp, Dept Dermatol, Sao Paulo, BrazilUniv Fed Sao Paulo Unifesp, Dept Dermatol, Sao Paulo, BrazilWeb of Science174-183engLepraLeprosy ReviewSerodiagnosis of leprosy and follow-up of household contacts using a commercial rapid test containing ND-O/LID-1 antigensinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleColchester882info:eu-repo/semantics/openAccessreponame:Repositório Institucional da UNIFESPinstname:Universidade Federal de São Paulo (UNIFESP)instacron:UNIFESPORIGINALWOS000411656500003.pdfapplication/pdf174864${dspace.ui.url}/bitstream/11600/53682/1/WOS000411656500003.pdf3ecfea067fec14dacda0134ca6b374c2MD51open accessTEXTWOS000411656500003.pdf.txtWOS000411656500003.pdf.txtExtracted texttext/plain33361${dspace.ui.url}/bitstream/11600/53682/2/WOS000411656500003.pdf.txt4607cd0e26df28b6ebdef13823aac0a8MD52open accessTHUMBNAILWOS000411656500003.pdf.jpgWOS000411656500003.pdf.jpgIM Thumbnailimage/jpeg5821${dspace.ui.url}/bitstream/11600/53682/4/WOS000411656500003.pdf.jpgecab006e081a7b56f3e335e8ad492db1MD54open access11600/536822022-08-01 09:41:46.394open accessoai:repositorio.unifesp.br:11600/53682Repositório InstitucionalPUBhttp://www.repositorio.unifesp.br/oai/requestopendoar:34652022-08-01T12:41:46Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)false |
dc.title.en.fl_str_mv |
Serodiagnosis of leprosy and follow-up of household contacts using a commercial rapid test containing ND-O/LID-1 antigens |
title |
Serodiagnosis of leprosy and follow-up of household contacts using a commercial rapid test containing ND-O/LID-1 antigens |
spellingShingle |
Serodiagnosis of leprosy and follow-up of household contacts using a commercial rapid test containing ND-O/LID-1 antigens Silva, Eliane Aparecida |
title_short |
Serodiagnosis of leprosy and follow-up of household contacts using a commercial rapid test containing ND-O/LID-1 antigens |
title_full |
Serodiagnosis of leprosy and follow-up of household contacts using a commercial rapid test containing ND-O/LID-1 antigens |
title_fullStr |
Serodiagnosis of leprosy and follow-up of household contacts using a commercial rapid test containing ND-O/LID-1 antigens |
title_full_unstemmed |
Serodiagnosis of leprosy and follow-up of household contacts using a commercial rapid test containing ND-O/LID-1 antigens |
title_sort |
Serodiagnosis of leprosy and follow-up of household contacts using a commercial rapid test containing ND-O/LID-1 antigens |
author |
Silva, Eliane Aparecida |
author_facet |
Silva, Eliane Aparecida Rosa, Patricia Sammarco Fernandes Belone, Andrea Faria Broch Coelho, Neusa Maria Ura, Somei Tomimori, Jane [UNIFESP] |
author_role |
author |
author2 |
Rosa, Patricia Sammarco Fernandes Belone, Andrea Faria Broch Coelho, Neusa Maria Ura, Somei Tomimori, Jane [UNIFESP] |
author2_role |
author author author author author |
dc.contributor.author.fl_str_mv |
Silva, Eliane Aparecida Rosa, Patricia Sammarco Fernandes Belone, Andrea Faria Broch Coelho, Neusa Maria Ura, Somei Tomimori, Jane [UNIFESP] |
description |
Introduction: Early diagnosis of leprosy, including the diagnosis of sub-clinical disease in contacts of known cases, would be a major advance. The signs of early leprosy are often difficult to assess and a reliable diagnostic test could play an important role in identifying cases and thus reducing transmission. Methods: Subjects were recruited at Centro de Saude Jardim Guanabara, Rondonopolis, Mato Grosso State, Brazil. Sera were obtained from 174 recently diagnosed leprosy patients, 409 household contacts, 53 endemic controls and 12 patients with active TB. 186 of the household contacts were re-tested on subsequent visits. Both the NDO-LID rapid test (Orange Life, Rio de Janeiro, Brazil) and an anti-PGL-1 ELISA test were used on all samples for comparison. Results: LID-1 was positive in 25 of 125 PB cases (20%), but in 41 of 49 MB cases (83.7%), while the anti-PGL-1 ELISA was positive in 8 (6.4%) and 35 (71.4%) cases, respectively. Specificity for LID-1 was 85% and for the anti-PGL-1 ELISA, 97%. Amongst household contacts, 9 of 409 (2.2%) were positive on the LID-1 test, and a further 51 (115%) were weakly positive |
publishDate |
2017 |
dc.date.issued.fl_str_mv |
2017 |
dc.date.accessioned.fl_str_mv |
2020-06-26T16:30:38Z |
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2020-06-26T16:30:38Z |
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info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
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publishedVersion |
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http://dx.doi.org/0305-7518/17/064053+10 |
dc.identifier.citation.fl_str_mv |
Leprosy Review. Colchester, v. 88, n. 2, p. 174-183, 2017. |
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https://repositorio.unifesp.br/handle/11600/53682 |
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0305-7518 |
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WOS000411656500003.pdf |
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0305-7518/17/064053+10 |
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WOS:000411656500003 |
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http://dx.doi.org/0305-7518/17/064053+10 https://repositorio.unifesp.br/handle/11600/53682 |
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Leprosy Review. Colchester, v. 88, n. 2, p. 174-183, 2017. 0305-7518 WOS000411656500003.pdf 0305-7518/17/064053+10 WOS:000411656500003 |
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