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Imunoexpressão de granzima B em linfócitos citotóxicos de linfonodos de pacientes com linfomas B agressivos e correlação de variáveis de prognósticoGranzimas.Imuno-Histoquímica.Linfoma Difuso de Grandes Células B.Linfoma de Hodgkin.Prognóstico.Granzyme B.Immunohistochemistry.Diffuse Large Cell Lymphoma.Hodgkin, Lymphoma.Prognosis.HematologiaIntrodução: Os linfócitos citotóxicos (linfócitos T CD8+ e células natural-killer) são capazes de induzir a apoptose de células infectadas por vírus e células transformadas através da secreção de grânulos citotóxicos ricos em granzima B. Dados sobre a infiltração destas células em amostras de linfomas B agressivos são controversos e sua relação com dados pré- tratamento, utilizados para fins de estadiamento, é pouco conhecida. Objetivos: Avaliar a expressão de granzima B em linfócitos no microambiente tumoral de linfoma Hodgkin clássico e de linfoma não-Hodgkin difuso de grandes células B e correlacionar estas informações com variáveis clínicas e de prognóstico, e resposta ao tratamento inicial. Métodos: Amostras de linfonodos comprometidos por linfoma Hodgkin ou linfoma nãoHodgkin difuso de grandes células B foram analisadas por técnica de imuno-histoquímica quanto à expressão de granzima B. Os resultados foram comparados com idade, gênero, estadiamento tumoral de Ann Arbor, presença de sintomas B, expressão de CD8, infiltração de medula óssea por linfoma, concentração sérica de desidrogenase láctica, concentração sérica de β2-microglobulina e resposta ao tratamento inicial. Resultados: A expressão de granzima B foi menor em amostras de pacientes com estadiamento tumoral avançado (p=0,025) e naqueles com medula óssea infiltrada por linfoma (p<0,0001). Houve correlação inversa entre a expressão de granzima B e níveis séricos de β2-microglobulina (p=0,0375; r=- 0,4270). Conclusão: A análise da expressão de granzima B em linfonodos comprometidos por linfomas B agressivos pode constituir ferramenta útil na aferição do prognóstico destes cânceres.Background: Cytotoxic lymphocytes (CD8+ T lymphocytes and natural killer cells) are able to induce apoptosis of virus-infected and transformed cells through the secretion of cytotoxic granules rich in granzyme B. Data on the infiltration of these cells into the tumor tissue of aggressive B lymphomas are controversial, and the relationship with pre-treatment data, used for staging purposes, is poorly understood. Thus, this study is aimed at evaluating the expression of granzyme B in lymphocytes in the classic Hodgkin’s lymphoma tumor and diffuse large B-cell non-Hodgkin's lymphoma and correlating this expression with clinical and prognostic variables as well as with the response to initial treatment. Methods: Samples of lymph nodes compromised by Hodgkin's lymphoma or diffuse large B-cell non-Hodgkin's lymphoma were analyzed by immunohistochemistry for granzyme B expression. Results were compared with respect to age, sex, Ann Arbor tumor staging, the presence of B-symptoms, CD8 expression, bone marrow infiltration by lymphoma, serum lactic dehydrogenase, serum β2-microglobulin, and response to initial treatment. Results: Granzyme B expression was lower in samples from patients with advanced tumor staging (p=0.025) and in those with bone marrow infiltrated by lymphoma (p<0.0001). An inverse correlation between granzyme B expression and serum β2-microglobulin levels (p=0.0375; r=-0.4270) was observed. Conclusion: Analysis of granzyme B expression in lymph nodes compromised by aggressive B lymphomas may be a useful prognosis tool for these cancers.Universidade Federal do Triângulo MineiroInstituto de Ciências da Saúde - ICS::Programa de Pós-Graduação em Ciências da SaúdeBrasilUFTMPrograma de Pós-Graduação em Ciências da SaúdeSOUZA, Helio Moraes10804528691http://lattes.cnpq.br/0502276939556083MENESES, Antonio Carlos de Oliveira01664468315http://lattes.cnpq.br/2709759848240540OLIVEIRA, Leonardo Rodrigues de2019-05-21T13:36:26Z2018-08-28info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisapplication/pdfapplication/pdfOLIVEIRA, Leonardo Rodrigues de. Imunoexpressão de granzima B em linfócitos citotóxicos de linfonodos de pacientes com linfomas B agressivos e correlação de variáveis de prognóstico. 2018. 67f. 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