Agronomic performance of cassava genotypes from the in vitro shoot tip culture submitted to clonal cleaning
Autor(a) principal: | |
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Data de Publicação: | 2017 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Bioscience journal (Online) |
Texto Completo: | https://seer.ufu.br/index.php/biosciencejournal/article/view/36585 |
Resumo: | This study aimed to evaluate the agronomic performance of cassava genotypes from the in vitro shoot tip culture to eliminate the cassava frogskin disease for several root and aerial part characteristics. Cassava plants from accessions BGM0315, BGM0464 and BGM0841 infected with cassava frogskin disease were grown in a greenhouse after clonal cleaning. Cuttings from the three accessions were subjected to tetracycline concentrations (0, 5, 10 and 15 mg L-1) for three minutes, and then maintained in an acclimatized chamber (35 ± 1 °C and 16 hour photoperiod). Shoots were disinfected for excising shoot tips (0.2 mm and 0.4 mm) and inoculated in a culture medium containing the same concentrations of tetracycline used in the cuttings. After 60 days of cultivation, the explants were transferred to medium without antibiotic, 30 days later they were acclimatized for a period of 70 days for subsequent planting in the field. Seven months after planting, agronomical evaluation was held for root and aerial part characteristics. No influence of isolated shoot tip size was noticed on agronomic characteristics, while the addition of tetracycline in the culture medium, specifically at the concentrations of 5 mg L-1 and 15 mg L-1, was favorable to the development of the root system of plants in the field. The results revealed that the agronomic performance of cassava plants derived from in vitro cultivation are higher for the production of basic propagation material for the following production cycles, as well as root production for commercial use with subsequent generation of income. |
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Agronomic performance of cassava genotypes from the in vitro shoot tip culture submitted to clonal cleaning Desempenho agronômico de genótipos de mandioca provenientes do cultivo in vitro de ápices caulinares submetidos à limpeza clonalManihot esculent CrantzIn vitro cultivationClonal cleaningStarch productionAgricultural SciencesThis study aimed to evaluate the agronomic performance of cassava genotypes from the in vitro shoot tip culture to eliminate the cassava frogskin disease for several root and aerial part characteristics. Cassava plants from accessions BGM0315, BGM0464 and BGM0841 infected with cassava frogskin disease were grown in a greenhouse after clonal cleaning. Cuttings from the three accessions were subjected to tetracycline concentrations (0, 5, 10 and 15 mg L-1) for three minutes, and then maintained in an acclimatized chamber (35 ± 1 °C and 16 hour photoperiod). Shoots were disinfected for excising shoot tips (0.2 mm and 0.4 mm) and inoculated in a culture medium containing the same concentrations of tetracycline used in the cuttings. After 60 days of cultivation, the explants were transferred to medium without antibiotic, 30 days later they were acclimatized for a period of 70 days for subsequent planting in the field. Seven months after planting, agronomical evaluation was held for root and aerial part characteristics. No influence of isolated shoot tip size was noticed on agronomic characteristics, while the addition of tetracycline in the culture medium, specifically at the concentrations of 5 mg L-1 and 15 mg L-1, was favorable to the development of the root system of plants in the field. The results revealed that the agronomic performance of cassava plants derived from in vitro cultivation are higher for the production of basic propagation material for the following production cycles, as well as root production for commercial use with subsequent generation of income.Este trabalho teve por objetivo avaliar o desempenho agronômico de genótipos de mandioca provenientes do cultivo in vitro de ápices caulinares para eliminação da doença couro de sapo (Cassava frogskin disease, CFSD), em relação a diversas características de raiz e da parte aérea. Para isso, foram utilizadas plantas de mandioca dos acessos BGM0315, BGM0464 e BGM0841 cultivadas em casa de vegetação após a limpeza clonal. Inicialmente, foram utilizadas manivas de plantas adultas infectadas com couro de sapo. Essas manivas foram submetidas a concentrações de tetraciclina (0, 5, 10 e 15 mg L-1) por 3 minutos. Posteriormente, foram mantidas em câmara climatizada (35 ± 1°C e fotoperíodo de 16 horas). Brotos foram desinfestados para excisão de ápices caulinares (0,2 mm e 0,4 mm) e inoculados em meio de cultura contendo as mesmas concentrações de tetraciclina empregadas nas manivas. Aos 60 dias de cultivo, os explantes foram transferidos para meio sem antibiótico, 30 dias após foram aclimatizadas por um período de 70 dias para posterior plantio em campo. Aos sete meses após o plantio realizou-se a avaliação agronômica para características de raiz e parte aérea. Não houve influência do tamanho do ápice caulinar isoladamente nas características agronômicas avaliadas, enquanto que a adição de tetraciclina no meio de cultura, especificamente nas concentrações de 5 e 15 mg L-1, foi favorável ao desenvolvimento do sistema radicular das plantas no campo. Os resultados evidenciaram que o desempenho agronômico de plantas de mandioca provenientes do cultivo in vitro são elevados para produção de material propagativo básico para os ciclos seguintes de produção, bem como para produção de raízes para uso comercial com consequente geração de renda.EDUFU2017-09-21info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://seer.ufu.br/index.php/biosciencejournal/article/view/3658510.14393/BJ-v33n5a2017-36585Bioscience Journal ; Vol. 33 No. 5 (2017): Sept./Oct.; 1144-1154Bioscience Journal ; v. 33 n. 5 (2017): Sept./Oct.; 1144-11541981-3163reponame:Bioscience journal (Online)instname:Universidade Federal de Uberlândia (UFU)instacron:UFUenghttps://seer.ufu.br/index.php/biosciencejournal/article/view/36585/20819Brazil; ContemporaryCopyright (c) 2017 Mariane de Jesus da Silva de Carvalho, Eder Jorge de Oliveira, Antônio da Silva Souza, Luziane Brandão Alves, Marcela Tonini Venturinihttps://creativecommons.org/licenses/by/4.0info:eu-repo/semantics/openAccessde Carvalho, Mariane de Jesus da SilvaOliveira, Eder Jorge deSouza, Antônio da SilvaAlves, Luziane BrandãoVenturini, Marcela Tonini2022-02-13T23:10:04Zoai:ojs.www.seer.ufu.br:article/36585Revistahttps://seer.ufu.br/index.php/biosciencejournalPUBhttps://seer.ufu.br/index.php/biosciencejournal/oaibiosciencej@ufu.br||1981-31631516-3725opendoar:2022-02-13T23:10:04Bioscience journal (Online) - Universidade Federal de Uberlândia (UFU)false |
dc.title.none.fl_str_mv |
Agronomic performance of cassava genotypes from the in vitro shoot tip culture submitted to clonal cleaning Desempenho agronômico de genótipos de mandioca provenientes do cultivo in vitro de ápices caulinares submetidos à limpeza clonal |
title |
Agronomic performance of cassava genotypes from the in vitro shoot tip culture submitted to clonal cleaning |
spellingShingle |
Agronomic performance of cassava genotypes from the in vitro shoot tip culture submitted to clonal cleaning de Carvalho, Mariane de Jesus da Silva Manihot esculent Crantz In vitro cultivation Clonal cleaning Starch production Agricultural Sciences |
title_short |
Agronomic performance of cassava genotypes from the in vitro shoot tip culture submitted to clonal cleaning |
title_full |
Agronomic performance of cassava genotypes from the in vitro shoot tip culture submitted to clonal cleaning |
title_fullStr |
Agronomic performance of cassava genotypes from the in vitro shoot tip culture submitted to clonal cleaning |
title_full_unstemmed |
Agronomic performance of cassava genotypes from the in vitro shoot tip culture submitted to clonal cleaning |
title_sort |
Agronomic performance of cassava genotypes from the in vitro shoot tip culture submitted to clonal cleaning |
author |
de Carvalho, Mariane de Jesus da Silva |
author_facet |
de Carvalho, Mariane de Jesus da Silva Oliveira, Eder Jorge de Souza, Antônio da Silva Alves, Luziane Brandão Venturini, Marcela Tonini |
author_role |
author |
author2 |
Oliveira, Eder Jorge de Souza, Antônio da Silva Alves, Luziane Brandão Venturini, Marcela Tonini |
author2_role |
author author author author |
dc.contributor.author.fl_str_mv |
de Carvalho, Mariane de Jesus da Silva Oliveira, Eder Jorge de Souza, Antônio da Silva Alves, Luziane Brandão Venturini, Marcela Tonini |
dc.subject.por.fl_str_mv |
Manihot esculent Crantz In vitro cultivation Clonal cleaning Starch production Agricultural Sciences |
topic |
Manihot esculent Crantz In vitro cultivation Clonal cleaning Starch production Agricultural Sciences |
description |
This study aimed to evaluate the agronomic performance of cassava genotypes from the in vitro shoot tip culture to eliminate the cassava frogskin disease for several root and aerial part characteristics. Cassava plants from accessions BGM0315, BGM0464 and BGM0841 infected with cassava frogskin disease were grown in a greenhouse after clonal cleaning. Cuttings from the three accessions were subjected to tetracycline concentrations (0, 5, 10 and 15 mg L-1) for three minutes, and then maintained in an acclimatized chamber (35 ± 1 °C and 16 hour photoperiod). Shoots were disinfected for excising shoot tips (0.2 mm and 0.4 mm) and inoculated in a culture medium containing the same concentrations of tetracycline used in the cuttings. After 60 days of cultivation, the explants were transferred to medium without antibiotic, 30 days later they were acclimatized for a period of 70 days for subsequent planting in the field. Seven months after planting, agronomical evaluation was held for root and aerial part characteristics. No influence of isolated shoot tip size was noticed on agronomic characteristics, while the addition of tetracycline in the culture medium, specifically at the concentrations of 5 mg L-1 and 15 mg L-1, was favorable to the development of the root system of plants in the field. The results revealed that the agronomic performance of cassava plants derived from in vitro cultivation are higher for the production of basic propagation material for the following production cycles, as well as root production for commercial use with subsequent generation of income. |
publishDate |
2017 |
dc.date.none.fl_str_mv |
2017-09-21 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://seer.ufu.br/index.php/biosciencejournal/article/view/36585 10.14393/BJ-v33n5a2017-36585 |
url |
https://seer.ufu.br/index.php/biosciencejournal/article/view/36585 |
identifier_str_mv |
10.14393/BJ-v33n5a2017-36585 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
https://seer.ufu.br/index.php/biosciencejournal/article/view/36585/20819 |
dc.rights.driver.fl_str_mv |
https://creativecommons.org/licenses/by/4.0 info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by/4.0 |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.coverage.none.fl_str_mv |
Brazil; Contemporary |
dc.publisher.none.fl_str_mv |
EDUFU |
publisher.none.fl_str_mv |
EDUFU |
dc.source.none.fl_str_mv |
Bioscience Journal ; Vol. 33 No. 5 (2017): Sept./Oct.; 1144-1154 Bioscience Journal ; v. 33 n. 5 (2017): Sept./Oct.; 1144-1154 1981-3163 reponame:Bioscience journal (Online) instname:Universidade Federal de Uberlândia (UFU) instacron:UFU |
instname_str |
Universidade Federal de Uberlândia (UFU) |
instacron_str |
UFU |
institution |
UFU |
reponame_str |
Bioscience journal (Online) |
collection |
Bioscience journal (Online) |
repository.name.fl_str_mv |
Bioscience journal (Online) - Universidade Federal de Uberlândia (UFU) |
repository.mail.fl_str_mv |
biosciencej@ufu.br|| |
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1797069077181628416 |