Multiplication, rooting in vitro, and acclimatization of Brassavola tuberculata Hook. (Orchidaceae), an orchid endemic to the brazilian atlantic rainforest

Detalhes bibliográficos
Autor(a) principal: Mengarda, Liana Hilda Golin
Data de Publicação: 2017
Outros Autores: Cola, Geovana Poton Arcobeli, de Oliveira, Stefanie Cristina, de Freitas, Allan Rocha
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Bioscience journal (Online)
Texto Completo: https://seer.ufu.br/index.php/biosciencejournal/article/view/32987
Resumo: The objective of this study was to promote the establishment of an in vitro culture of Brassavola tuberculata, testing different concentrations of naphthalene acetic acid (NAA) and 6-benzylaminopurine (BAP) on multiplication and rooting, evaluating different substrates during acclimatization, as well as the effect of in vitro treatments. After germination, the seedlings of B. tuberculata were subjected to culture on MS medium supplemented with different concentrations of NAA and BAP, and multiplication and rooting were assessed. During acclimatization, different substrates were tested: S1, Plantmax® and vermiculite (1: 1); S2, Plantmax® and grit (1: 1); and S3, dust fern. Also the effect of the in vitro culture treatments was evaluated: T1, control; T5, (2.5 µM NAA +5 µM BAP); and T7, (5 µM NAA + 0 µM BAP). The favorable balance of cytokinins promoted by treatment T5 yielded the largest number of shoots and leaves in B. tuberculata. The greatest length of leaves and roots, and highest root number were observed in the treatment T7, favored by the presence of auxin. This treatment had a positive effect with respect to plant acclimatization: T7 associated with substrate S1 provided the most suitable conditions for acclimatization of seedlings of B. tuberculata, providing greater number and length of leaves, and high survival rate.
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spelling Multiplication, rooting in vitro, and acclimatization of Brassavola tuberculata Hook. (Orchidaceae), an orchid endemic to the brazilian atlantic rainforest Multiplicação, enraizamento in vitro e aclimatização de Brassavola tuberculata Hook (Orchidaceae), uma orquídea endêmica da mata atlântica brasileira 6-benzylaminopurineIn vitro establishmentNephathalene acetic acidSubstratesTissue cultureBiological SciencesThe objective of this study was to promote the establishment of an in vitro culture of Brassavola tuberculata, testing different concentrations of naphthalene acetic acid (NAA) and 6-benzylaminopurine (BAP) on multiplication and rooting, evaluating different substrates during acclimatization, as well as the effect of in vitro treatments. After germination, the seedlings of B. tuberculata were subjected to culture on MS medium supplemented with different concentrations of NAA and BAP, and multiplication and rooting were assessed. During acclimatization, different substrates were tested: S1, Plantmax® and vermiculite (1: 1); S2, Plantmax® and grit (1: 1); and S3, dust fern. Also the effect of the in vitro culture treatments was evaluated: T1, control; T5, (2.5 µM NAA +5 µM BAP); and T7, (5 µM NAA + 0 µM BAP). The favorable balance of cytokinins promoted by treatment T5 yielded the largest number of shoots and leaves in B. tuberculata. The greatest length of leaves and roots, and highest root number were observed in the treatment T7, favored by the presence of auxin. This treatment had a positive effect with respect to plant acclimatization: T7 associated with substrate S1 provided the most suitable conditions for acclimatization of seedlings of B. tuberculata, providing greater number and length of leaves, and high survival rate.Objetivou-se, com este trabalho, promover o estabelecimento do cultivo in vitro de Brassavola tuberculata, testando diferentes concentrações de ANA e BAP na multiplicação e no enraizamento, e avaliar diferentessubstratos e o efeito dos tratamentos de cultivo in vitro na aclimatização. Após a germinação das sementes, as plântulas de B. tuberculata foram submetidas ao cultivo em meio MS suplementado com diferentes concentrações de ácido naftaleno acético (ANA) e 6-benzilaminopurina (BAP), sendo avaliados a multiplicação e o enraizamento. Foram testados diferentes substratos: S1 (Plantmax e vermiculita (1:1)); S2 (Plantmax e areia (1:1)) e S3 (pó de xaxim) na aclimatização e, posteriormente, o efeito dos tratamentos do cultivo in vitro: T1 (controle), T5 (2,5 ANA + 5 BAP) e T7 (5 ANA + 0 BAP), na aclimatização. O balanço favorável às citocininas promovido pelo tratamento T5 (2,5 µM ANA + 5 µM BAP) promoveu maior número de brotos e de folhas em B. tuberculata. O maior comprimento das folhas, das raízes e maior número de raízes foi observado no tratamento T7 (5 µM ANA e 0 µM BAP), favorável a auxina. Este tratamento apresentou efeito positivo com relação a aclimatização das plantas: T7 associado ao substrato S1, Plantmax e vermiculita (1:1) proporcionou melhores condições para a aclimatização das plântulas de B. tuberculata, propiciando maior número e comprimento das folhas, e elevada taxa de sobrevivência.  EDUFU2017-05-24info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://seer.ufu.br/index.php/biosciencejournal/article/view/3298710.14393/BJ-v33n3-32987Bioscience Journal ; Vol. 33 No. 3 (2017): May/June; 730-738Bioscience Journal ; v. 33 n. 3 (2017): May./June; 730-7381981-3163reponame:Bioscience journal (Online)instname:Universidade Federal de Uberlândia (UFU)instacron:UFUenghttps://seer.ufu.br/index.php/biosciencejournal/article/view/32987/20336Brazil; ContemporaryCopyright (c) 2017 Liana Hilda Golin Mengarda, Geovana Poton Arcobeli Cola, Stefanie Cristina de Oliveira, Allan Rocha de Freitashttps://creativecommons.org/licenses/by/4.0info:eu-repo/semantics/openAccessMengarda, Liana Hilda GolinCola, Geovana Poton Arcobelide Oliveira, Stefanie Cristinade Freitas, Allan Rocha2022-02-16T02:03:10Zoai:ojs.www.seer.ufu.br:article/32987Revistahttps://seer.ufu.br/index.php/biosciencejournalPUBhttps://seer.ufu.br/index.php/biosciencejournal/oaibiosciencej@ufu.br||1981-31631516-3725opendoar:2022-02-16T02:03:10Bioscience journal (Online) - Universidade Federal de Uberlândia (UFU)false
dc.title.none.fl_str_mv Multiplication, rooting in vitro, and acclimatization of Brassavola tuberculata Hook. (Orchidaceae), an orchid endemic to the brazilian atlantic rainforest
Multiplicação, enraizamento in vitro e aclimatização de Brassavola tuberculata Hook (Orchidaceae), uma orquídea endêmica da mata atlântica brasileira
title Multiplication, rooting in vitro, and acclimatization of Brassavola tuberculata Hook. (Orchidaceae), an orchid endemic to the brazilian atlantic rainforest
spellingShingle Multiplication, rooting in vitro, and acclimatization of Brassavola tuberculata Hook. (Orchidaceae), an orchid endemic to the brazilian atlantic rainforest
Mengarda, Liana Hilda Golin
6-benzylaminopurine
In vitro establishment
Nephathalene acetic acid
Substrates
Tissue culture
Biological Sciences
title_short Multiplication, rooting in vitro, and acclimatization of Brassavola tuberculata Hook. (Orchidaceae), an orchid endemic to the brazilian atlantic rainforest
title_full Multiplication, rooting in vitro, and acclimatization of Brassavola tuberculata Hook. (Orchidaceae), an orchid endemic to the brazilian atlantic rainforest
title_fullStr Multiplication, rooting in vitro, and acclimatization of Brassavola tuberculata Hook. (Orchidaceae), an orchid endemic to the brazilian atlantic rainforest
title_full_unstemmed Multiplication, rooting in vitro, and acclimatization of Brassavola tuberculata Hook. (Orchidaceae), an orchid endemic to the brazilian atlantic rainforest
title_sort Multiplication, rooting in vitro, and acclimatization of Brassavola tuberculata Hook. (Orchidaceae), an orchid endemic to the brazilian atlantic rainforest
author Mengarda, Liana Hilda Golin
author_facet Mengarda, Liana Hilda Golin
Cola, Geovana Poton Arcobeli
de Oliveira, Stefanie Cristina
de Freitas, Allan Rocha
author_role author
author2 Cola, Geovana Poton Arcobeli
de Oliveira, Stefanie Cristina
de Freitas, Allan Rocha
author2_role author
author
author
dc.contributor.author.fl_str_mv Mengarda, Liana Hilda Golin
Cola, Geovana Poton Arcobeli
de Oliveira, Stefanie Cristina
de Freitas, Allan Rocha
dc.subject.por.fl_str_mv 6-benzylaminopurine
In vitro establishment
Nephathalene acetic acid
Substrates
Tissue culture
Biological Sciences
topic 6-benzylaminopurine
In vitro establishment
Nephathalene acetic acid
Substrates
Tissue culture
Biological Sciences
description The objective of this study was to promote the establishment of an in vitro culture of Brassavola tuberculata, testing different concentrations of naphthalene acetic acid (NAA) and 6-benzylaminopurine (BAP) on multiplication and rooting, evaluating different substrates during acclimatization, as well as the effect of in vitro treatments. After germination, the seedlings of B. tuberculata were subjected to culture on MS medium supplemented with different concentrations of NAA and BAP, and multiplication and rooting were assessed. During acclimatization, different substrates were tested: S1, Plantmax® and vermiculite (1: 1); S2, Plantmax® and grit (1: 1); and S3, dust fern. Also the effect of the in vitro culture treatments was evaluated: T1, control; T5, (2.5 µM NAA +5 µM BAP); and T7, (5 µM NAA + 0 µM BAP). The favorable balance of cytokinins promoted by treatment T5 yielded the largest number of shoots and leaves in B. tuberculata. The greatest length of leaves and roots, and highest root number were observed in the treatment T7, favored by the presence of auxin. This treatment had a positive effect with respect to plant acclimatization: T7 associated with substrate S1 provided the most suitable conditions for acclimatization of seedlings of B. tuberculata, providing greater number and length of leaves, and high survival rate.
publishDate 2017
dc.date.none.fl_str_mv 2017-05-24
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://seer.ufu.br/index.php/biosciencejournal/article/view/32987
10.14393/BJ-v33n3-32987
url https://seer.ufu.br/index.php/biosciencejournal/article/view/32987
identifier_str_mv 10.14393/BJ-v33n3-32987
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv https://seer.ufu.br/index.php/biosciencejournal/article/view/32987/20336
dc.rights.driver.fl_str_mv https://creativecommons.org/licenses/by/4.0
info:eu-repo/semantics/openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/4.0
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.coverage.none.fl_str_mv Brazil; Contemporary
dc.publisher.none.fl_str_mv EDUFU
publisher.none.fl_str_mv EDUFU
dc.source.none.fl_str_mv Bioscience Journal ; Vol. 33 No. 3 (2017): May/June; 730-738
Bioscience Journal ; v. 33 n. 3 (2017): May./June; 730-738
1981-3163
reponame:Bioscience journal (Online)
instname:Universidade Federal de Uberlândia (UFU)
instacron:UFU
instname_str Universidade Federal de Uberlândia (UFU)
instacron_str UFU
institution UFU
reponame_str Bioscience journal (Online)
collection Bioscience journal (Online)
repository.name.fl_str_mv Bioscience journal (Online) - Universidade Federal de Uberlândia (UFU)
repository.mail.fl_str_mv biosciencej@ufu.br||
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