First detection of Leishmania of the subgenus viannia in Alipiopsitta xanthops, endemic bird of South America
Autor(a) principal: | |
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Data de Publicação: | 2021 |
Outros Autores: | , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Bioscience journal (Online) |
Texto Completo: | https://seer.ufu.br/index.php/biosciencejournal/article/view/48024 |
Resumo: | We report the first molecular detection of Leishmania infection (subgenus Viannia) in the yellow-faced parrot (Alipiopsitta xanthops), at a wildlife rehabilitation center located in the city of Campo Grande, Brazil, an endemic area for leishmaniasis. PCRs targeting kinetoplast DNA (kDNA) and the small subunit of ribosomal RNA of Leishmania spp. were performed, both positive, followed by the sequencing of the amplified region of the SSU rDNA gene, which confirmed the identity of the parasite. This is the first report of success obtained in the use of PCR targeting the IRBP (Interphotoreceptor retinoid-binding protein) gene as an internal control in the molecular diagnosis of pathogens in bird species. |
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First detection of Leishmania of the subgenus viannia in Alipiopsitta xanthops, endemic bird of South AmericaBirdsIRBPLeishmaniaPCRrDNASSU.VeterinaryWe report the first molecular detection of Leishmania infection (subgenus Viannia) in the yellow-faced parrot (Alipiopsitta xanthops), at a wildlife rehabilitation center located in the city of Campo Grande, Brazil, an endemic area for leishmaniasis. PCRs targeting kinetoplast DNA (kDNA) and the small subunit of ribosomal RNA of Leishmania spp. were performed, both positive, followed by the sequencing of the amplified region of the SSU rDNA gene, which confirmed the identity of the parasite. This is the first report of success obtained in the use of PCR targeting the IRBP (Interphotoreceptor retinoid-binding protein) gene as an internal control in the molecular diagnosis of pathogens in bird species. EDUFU2021-02-25info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://seer.ufu.br/index.php/biosciencejournal/article/view/4802410.14393/BJ-v37n0a2021-48024Bioscience Journal ; Vol. 37 (2021): Continuous Publication; e37014Bioscience Journal ; v. 37 (2021): Continuous Publication; e370141981-3163reponame:Bioscience journal (Online)instname:Universidade Federal de Uberlândia (UFU)instacron:UFUenghttps://seer.ufu.br/index.php/biosciencejournal/article/view/48024/31030Brazil; ContemporaryCopyright (c) 2021 Larissa Maria Dávalo Matheus, Pâmella Oliveira Duarte, Eduardo de Castro Ferreirahttps://creativecommons.org/licenses/by/4.0info:eu-repo/semantics/openAccessDávalo Matheus, Larissa MariaDuarte, Pâmella Oliveira Ferreira, Eduardo de Castro 2022-05-25T11:49:14Zoai:ojs.www.seer.ufu.br:article/48024Revistahttps://seer.ufu.br/index.php/biosciencejournalPUBhttps://seer.ufu.br/index.php/biosciencejournal/oaibiosciencej@ufu.br||1981-31631516-3725opendoar:2022-05-25T11:49:14Bioscience journal (Online) - Universidade Federal de Uberlândia (UFU)false |
dc.title.none.fl_str_mv |
First detection of Leishmania of the subgenus viannia in Alipiopsitta xanthops, endemic bird of South America |
title |
First detection of Leishmania of the subgenus viannia in Alipiopsitta xanthops, endemic bird of South America |
spellingShingle |
First detection of Leishmania of the subgenus viannia in Alipiopsitta xanthops, endemic bird of South America Dávalo Matheus, Larissa Maria Birds IRBP Leishmania PCR rDNA SSU. Veterinary |
title_short |
First detection of Leishmania of the subgenus viannia in Alipiopsitta xanthops, endemic bird of South America |
title_full |
First detection of Leishmania of the subgenus viannia in Alipiopsitta xanthops, endemic bird of South America |
title_fullStr |
First detection of Leishmania of the subgenus viannia in Alipiopsitta xanthops, endemic bird of South America |
title_full_unstemmed |
First detection of Leishmania of the subgenus viannia in Alipiopsitta xanthops, endemic bird of South America |
title_sort |
First detection of Leishmania of the subgenus viannia in Alipiopsitta xanthops, endemic bird of South America |
author |
Dávalo Matheus, Larissa Maria |
author_facet |
Dávalo Matheus, Larissa Maria Duarte, Pâmella Oliveira Ferreira, Eduardo de Castro |
author_role |
author |
author2 |
Duarte, Pâmella Oliveira Ferreira, Eduardo de Castro |
author2_role |
author author |
dc.contributor.author.fl_str_mv |
Dávalo Matheus, Larissa Maria Duarte, Pâmella Oliveira Ferreira, Eduardo de Castro |
dc.subject.por.fl_str_mv |
Birds IRBP Leishmania PCR rDNA SSU. Veterinary |
topic |
Birds IRBP Leishmania PCR rDNA SSU. Veterinary |
description |
We report the first molecular detection of Leishmania infection (subgenus Viannia) in the yellow-faced parrot (Alipiopsitta xanthops), at a wildlife rehabilitation center located in the city of Campo Grande, Brazil, an endemic area for leishmaniasis. PCRs targeting kinetoplast DNA (kDNA) and the small subunit of ribosomal RNA of Leishmania spp. were performed, both positive, followed by the sequencing of the amplified region of the SSU rDNA gene, which confirmed the identity of the parasite. This is the first report of success obtained in the use of PCR targeting the IRBP (Interphotoreceptor retinoid-binding protein) gene as an internal control in the molecular diagnosis of pathogens in bird species. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-02-25 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://seer.ufu.br/index.php/biosciencejournal/article/view/48024 10.14393/BJ-v37n0a2021-48024 |
url |
https://seer.ufu.br/index.php/biosciencejournal/article/view/48024 |
identifier_str_mv |
10.14393/BJ-v37n0a2021-48024 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
https://seer.ufu.br/index.php/biosciencejournal/article/view/48024/31030 |
dc.rights.driver.fl_str_mv |
https://creativecommons.org/licenses/by/4.0 info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by/4.0 |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.coverage.none.fl_str_mv |
Brazil; Contemporary |
dc.publisher.none.fl_str_mv |
EDUFU |
publisher.none.fl_str_mv |
EDUFU |
dc.source.none.fl_str_mv |
Bioscience Journal ; Vol. 37 (2021): Continuous Publication; e37014 Bioscience Journal ; v. 37 (2021): Continuous Publication; e37014 1981-3163 reponame:Bioscience journal (Online) instname:Universidade Federal de Uberlândia (UFU) instacron:UFU |
instname_str |
Universidade Federal de Uberlândia (UFU) |
instacron_str |
UFU |
institution |
UFU |
reponame_str |
Bioscience journal (Online) |
collection |
Bioscience journal (Online) |
repository.name.fl_str_mv |
Bioscience journal (Online) - Universidade Federal de Uberlândia (UFU) |
repository.mail.fl_str_mv |
biosciencej@ufu.br|| |
_version_ |
1797069081733496832 |