Selection and characterization of Bacillus thuringiensis isolates with a high insecticidal activity against Spodoptera frugiperda (Lepidoptera: Noctuidae)
Autor(a) principal: | |
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Data de Publicação: | 2016 |
Outros Autores: | , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Bioscience journal (Online) |
Texto Completo: | https://seer.ufu.br/index.php/biosciencejournal/article/view/32741 |
Resumo: | Spodoptera frugiperda (SMITH, 1797) (Lepidoptera: Noctuidae) affects diverse crops of great economic interest, for instance, it can cause severe yield losses in maize, rice and sorghum. In this study, a selection and characterization of Bacillus thuringiensis (BERLINER, 1911) isolates with a high insecticidal activity against S. frugiperda was performed. Fifty-two crystal-forming B. thuringiensis isolates that were identified from 3384 Bacillus-like colonies were examined and screened by PCR for the presence cry genes (cry1, cry1Aa, cry1Ab, cry1Ac, cry1D, cry2 and cry2Ab). Four isolates that showed high toxicity towards S. frugiperda were shown to harbor cry2 genes. The crystals were analyzed by electron microscopy and showed bipyramidal and cuboidal shapes. Furthermore, these four isolates had lethal concentration (LC50) values of 44.5 ng/cm2 (SUFT01), 74.0 ng/cm2 (SUFT02), 89.0 ng/cm2 (SUFT03) and 108 ng/cm2 (SUFT 04) to neonate S. frugiperda larvae. An ultrastructural analysis of midgut cells from S. frugiperda incubated with the SUFT01 spore-crystal complex showed disruptions in cellular integrity and in the microvilli of the midgut columnar cells. The isolates characterized in this work are good candidates for the control of S. frugiperda, and could  be used for the formulation of new bioinsecticides. |
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Selection and characterization of Bacillus thuringiensis isolates with a high insecticidal activity against Spodoptera frugiperda (Lepidoptera: Noctuidae) Cry proteinLepidopteranEntomopathogenMicrobial insecticideInsect controlAgricultural SciencesSpodoptera frugiperda (SMITH, 1797) (Lepidoptera: Noctuidae) affects diverse crops of great economic interest, for instance, it can cause severe yield losses in maize, rice and sorghum. In this study, a selection and characterization of Bacillus thuringiensis (BERLINER, 1911) isolates with a high insecticidal activity against S. frugiperda was performed. Fifty-two crystal-forming B. thuringiensis isolates that were identified from 3384 Bacillus-like colonies were examined and screened by PCR for the presence cry genes (cry1, cry1Aa, cry1Ab, cry1Ac, cry1D, cry2 and cry2Ab). Four isolates that showed high toxicity towards S. frugiperda were shown to harbor cry2 genes. The crystals were analyzed by electron microscopy and showed bipyramidal and cuboidal shapes. Furthermore, these four isolates had lethal concentration (LC50) values of 44.5 ng/cm2 (SUFT01), 74.0 ng/cm2 (SUFT02), 89.0 ng/cm2 (SUFT03) and 108 ng/cm2 (SUFT 04) to neonate S. frugiperda larvae. An ultrastructural analysis of midgut cells from S. frugiperda incubated with the SUFT01 spore-crystal complex showed disruptions in cellular integrity and in the microvilli of the midgut columnar cells. The isolates characterized in this work are good candidates for the control of S. frugiperda, and could  be used for the formulation of new bioinsecticides.EDUFU2016-12-06info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://seer.ufu.br/index.php/biosciencejournal/article/view/3274110.14393/BJ-v32n6a2016-32741Bioscience Journal ; Vol. 32 No. 6 (2016): Nov./Dec.; 1522-1536Bioscience Journal ; v. 32 n. 6 (2016): Nov./Dec.; 1522-15361981-3163reponame:Bioscience journal (Online)instname:Universidade Federal de Uberlândia (UFU)instacron:UFUenghttps://seer.ufu.br/index.php/biosciencejournal/article/view/32741/19351Brazil; ContemporaryCopyright (c) 2016 Fernando Barnabe Cerqueira, Giselly Batista Alves, Roberto Franco Teixeira Corrêa, Soares Martins, Luiz Carlos Bertucci Barbosa, Ildon Rodrigues do Nascimento, Rose Gomes Monnerat, Bergmann Morais Ribeiro, Raimundo Wagner de Souza Aguiarhttps://creativecommons.org/licenses/by/4.0info:eu-repo/semantics/openAccessCerqueira, Fernando BarnabeAlves, Giselly BatistaCorrêa, Roberto Franco TeixeiraMartins, SoaresBarbosa, Luiz Carlos Bertuccido Nascimento, Ildon RodriguesMonnerat, Rose GomesRibeiro, Bergmann MoraisAguiar, Raimundo Wagner de Souza2022-02-16T18:04:27Zoai:ojs.www.seer.ufu.br:article/32741Revistahttps://seer.ufu.br/index.php/biosciencejournalPUBhttps://seer.ufu.br/index.php/biosciencejournal/oaibiosciencej@ufu.br||1981-31631516-3725opendoar:2022-02-16T18:04:27Bioscience journal (Online) - Universidade Federal de Uberlândia (UFU)false |
dc.title.none.fl_str_mv |
Selection and characterization of Bacillus thuringiensis isolates with a high insecticidal activity against Spodoptera frugiperda (Lepidoptera: Noctuidae) |
title |
Selection and characterization of Bacillus thuringiensis isolates with a high insecticidal activity against Spodoptera frugiperda (Lepidoptera: Noctuidae) |
spellingShingle |
Selection and characterization of Bacillus thuringiensis isolates with a high insecticidal activity against Spodoptera frugiperda (Lepidoptera: Noctuidae) Cerqueira, Fernando Barnabe Cry protein Lepidopteran Entomopathogen Microbial insecticide Insect control Agricultural Sciences |
title_short |
Selection and characterization of Bacillus thuringiensis isolates with a high insecticidal activity against Spodoptera frugiperda (Lepidoptera: Noctuidae) |
title_full |
Selection and characterization of Bacillus thuringiensis isolates with a high insecticidal activity against Spodoptera frugiperda (Lepidoptera: Noctuidae) |
title_fullStr |
Selection and characterization of Bacillus thuringiensis isolates with a high insecticidal activity against Spodoptera frugiperda (Lepidoptera: Noctuidae) |
title_full_unstemmed |
Selection and characterization of Bacillus thuringiensis isolates with a high insecticidal activity against Spodoptera frugiperda (Lepidoptera: Noctuidae) |
title_sort |
Selection and characterization of Bacillus thuringiensis isolates with a high insecticidal activity against Spodoptera frugiperda (Lepidoptera: Noctuidae) |
author |
Cerqueira, Fernando Barnabe |
author_facet |
Cerqueira, Fernando Barnabe Alves, Giselly Batista Corrêa, Roberto Franco Teixeira Martins, Soares Barbosa, Luiz Carlos Bertucci do Nascimento, Ildon Rodrigues Monnerat, Rose Gomes Ribeiro, Bergmann Morais Aguiar, Raimundo Wagner de Souza |
author_role |
author |
author2 |
Alves, Giselly Batista Corrêa, Roberto Franco Teixeira Martins, Soares Barbosa, Luiz Carlos Bertucci do Nascimento, Ildon Rodrigues Monnerat, Rose Gomes Ribeiro, Bergmann Morais Aguiar, Raimundo Wagner de Souza |
author2_role |
author author author author author author author author |
dc.contributor.author.fl_str_mv |
Cerqueira, Fernando Barnabe Alves, Giselly Batista Corrêa, Roberto Franco Teixeira Martins, Soares Barbosa, Luiz Carlos Bertucci do Nascimento, Ildon Rodrigues Monnerat, Rose Gomes Ribeiro, Bergmann Morais Aguiar, Raimundo Wagner de Souza |
dc.subject.por.fl_str_mv |
Cry protein Lepidopteran Entomopathogen Microbial insecticide Insect control Agricultural Sciences |
topic |
Cry protein Lepidopteran Entomopathogen Microbial insecticide Insect control Agricultural Sciences |
description |
Spodoptera frugiperda (SMITH, 1797) (Lepidoptera: Noctuidae) affects diverse crops of great economic interest, for instance, it can cause severe yield losses in maize, rice and sorghum. In this study, a selection and characterization of Bacillus thuringiensis (BERLINER, 1911) isolates with a high insecticidal activity against S. frugiperda was performed. Fifty-two crystal-forming B. thuringiensis isolates that were identified from 3384 Bacillus-like colonies were examined and screened by PCR for the presence cry genes (cry1, cry1Aa, cry1Ab, cry1Ac, cry1D, cry2 and cry2Ab). Four isolates that showed high toxicity towards S. frugiperda were shown to harbor cry2 genes. The crystals were analyzed by electron microscopy and showed bipyramidal and cuboidal shapes. Furthermore, these four isolates had lethal concentration (LC50) values of 44.5 ng/cm2 (SUFT01), 74.0 ng/cm2 (SUFT02), 89.0 ng/cm2 (SUFT03) and 108 ng/cm2 (SUFT 04) to neonate S. frugiperda larvae. An ultrastructural analysis of midgut cells from S. frugiperda incubated with the SUFT01 spore-crystal complex showed disruptions in cellular integrity and in the microvilli of the midgut columnar cells. The isolates characterized in this work are good candidates for the control of S. frugiperda, and could  be used for the formulation of new bioinsecticides. |
publishDate |
2016 |
dc.date.none.fl_str_mv |
2016-12-06 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://seer.ufu.br/index.php/biosciencejournal/article/view/32741 10.14393/BJ-v32n6a2016-32741 |
url |
https://seer.ufu.br/index.php/biosciencejournal/article/view/32741 |
identifier_str_mv |
10.14393/BJ-v32n6a2016-32741 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
https://seer.ufu.br/index.php/biosciencejournal/article/view/32741/19351 |
dc.rights.driver.fl_str_mv |
https://creativecommons.org/licenses/by/4.0 info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by/4.0 |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.coverage.none.fl_str_mv |
Brazil; Contemporary |
dc.publisher.none.fl_str_mv |
EDUFU |
publisher.none.fl_str_mv |
EDUFU |
dc.source.none.fl_str_mv |
Bioscience Journal ; Vol. 32 No. 6 (2016): Nov./Dec.; 1522-1536 Bioscience Journal ; v. 32 n. 6 (2016): Nov./Dec.; 1522-1536 1981-3163 reponame:Bioscience journal (Online) instname:Universidade Federal de Uberlândia (UFU) instacron:UFU |
instname_str |
Universidade Federal de Uberlândia (UFU) |
instacron_str |
UFU |
institution |
UFU |
reponame_str |
Bioscience journal (Online) |
collection |
Bioscience journal (Online) |
repository.name.fl_str_mv |
Bioscience journal (Online) - Universidade Federal de Uberlândia (UFU) |
repository.mail.fl_str_mv |
biosciencej@ufu.br|| |
_version_ |
1797069076327038976 |