Effects of exogenous proline and a natural ventilation system on the in vitro growth of orchids
Autor(a) principal: | |
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Data de Publicação: | 2016 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Bioscience journal (Online) |
Texto Completo: | https://seer.ufu.br/index.php/biosciencejournal/article/view/31368 |
Resumo: | Micropropagation is an alternative to produce orchid plants in large scale. However, this process presents losses during acclimatization. Exogenous proline use in vitro plant tissue culture can reduce the stress of the plant acclimatization phase. We aimed to verify the growth of orchids in different micropropagation systems with the addition of proline in the culture medium. Cattleya walkeriana plants were obtained from the germination of seeds in culture medium. Seeds were germinated in MS medium, added 20 g. L-1 of sucrose, solidified with 6 g. L-1 of agar and pH adjusted for 5,8. The cultures were incubated in a growth room with temperature of 24 ± 2 0C, under photoperiod of 16 h. After 5 months, 1-cm long seedlings were placed in a culture vessel according to the treatments, which were composed of two micropropagation systems (conventional and natural ventilation) and three proline concentrations (0, 1, and 2 g·L-1). The experiment was carried out in an entirely randomized design consisting of a 2 × 3 factorial, for a total of 6 treatments, each with 5 replicates. The natural ventilation system with the use of proline (1 g·L-1) promoted higher dry mass accumulation and better control of water loss by plants. |
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Effects of exogenous proline and a natural ventilation system on the in vitro growth of orchids OrquídeasMicropropagaçãoEstresse abióticoAgricultural SciencesMicropropagation is an alternative to produce orchid plants in large scale. However, this process presents losses during acclimatization. Exogenous proline use in vitro plant tissue culture can reduce the stress of the plant acclimatization phase. We aimed to verify the growth of orchids in different micropropagation systems with the addition of proline in the culture medium. Cattleya walkeriana plants were obtained from the germination of seeds in culture medium. Seeds were germinated in MS medium, added 20 g. L-1 of sucrose, solidified with 6 g. L-1 of agar and pH adjusted for 5,8. The cultures were incubated in a growth room with temperature of 24 ± 2 0C, under photoperiod of 16 h. After 5 months, 1-cm long seedlings were placed in a culture vessel according to the treatments, which were composed of two micropropagation systems (conventional and natural ventilation) and three proline concentrations (0, 1, and 2 g·L-1). The experiment was carried out in an entirely randomized design consisting of a 2 × 3 factorial, for a total of 6 treatments, each with 5 replicates. The natural ventilation system with the use of proline (1 g·L-1) promoted higher dry mass accumulation and better control of water loss by plants.EDUFU2016-06-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://seer.ufu.br/index.php/biosciencejournal/article/view/3136810.14393/BJ-v32n3a2016-31368Bioscience Journal ; Vol. 32 No. 3 (2016): May/June; 619-626Bioscience Journal ; v. 32 n. 3 (2016): May/June; 619-6261981-3163reponame:Bioscience journal (Online)instname:Universidade Federal de Uberlândia (UFU)instacron:UFUenghttps://seer.ufu.br/index.php/biosciencejournal/article/view/31368/18304Brazil; ContemporaryCopyright (c) 2016 Adriano Bortolotti da Silva, Caroline Oliveira dos Reis, Jairo Osvaldo Cazetta, Samira Domingues Carlin, Paulo Roberto Corrêa Landgraf, Marina Cunha dos Reishttps://creativecommons.org/licenses/by/4.0info:eu-repo/semantics/openAccessSilva, Adriano Bortolotti daReis, Caroline Oliveira dosCazetta, Jairo OsvaldoCarlin, Samira DominguesLandgraf, Paulo Roberto CorrêaReis, Marina Cunha dos2022-02-23T01:34:48Zoai:ojs.www.seer.ufu.br:article/31368Revistahttps://seer.ufu.br/index.php/biosciencejournalPUBhttps://seer.ufu.br/index.php/biosciencejournal/oaibiosciencej@ufu.br||1981-31631516-3725opendoar:2022-02-23T01:34:48Bioscience journal (Online) - Universidade Federal de Uberlândia (UFU)false |
dc.title.none.fl_str_mv |
Effects of exogenous proline and a natural ventilation system on the in vitro growth of orchids |
title |
Effects of exogenous proline and a natural ventilation system on the in vitro growth of orchids |
spellingShingle |
Effects of exogenous proline and a natural ventilation system on the in vitro growth of orchids Silva, Adriano Bortolotti da Orquídeas Micropropagação Estresse abiótico Agricultural Sciences |
title_short |
Effects of exogenous proline and a natural ventilation system on the in vitro growth of orchids |
title_full |
Effects of exogenous proline and a natural ventilation system on the in vitro growth of orchids |
title_fullStr |
Effects of exogenous proline and a natural ventilation system on the in vitro growth of orchids |
title_full_unstemmed |
Effects of exogenous proline and a natural ventilation system on the in vitro growth of orchids |
title_sort |
Effects of exogenous proline and a natural ventilation system on the in vitro growth of orchids |
author |
Silva, Adriano Bortolotti da |
author_facet |
Silva, Adriano Bortolotti da Reis, Caroline Oliveira dos Cazetta, Jairo Osvaldo Carlin, Samira Domingues Landgraf, Paulo Roberto Corrêa Reis, Marina Cunha dos |
author_role |
author |
author2 |
Reis, Caroline Oliveira dos Cazetta, Jairo Osvaldo Carlin, Samira Domingues Landgraf, Paulo Roberto Corrêa Reis, Marina Cunha dos |
author2_role |
author author author author author |
dc.contributor.author.fl_str_mv |
Silva, Adriano Bortolotti da Reis, Caroline Oliveira dos Cazetta, Jairo Osvaldo Carlin, Samira Domingues Landgraf, Paulo Roberto Corrêa Reis, Marina Cunha dos |
dc.subject.por.fl_str_mv |
Orquídeas Micropropagação Estresse abiótico Agricultural Sciences |
topic |
Orquídeas Micropropagação Estresse abiótico Agricultural Sciences |
description |
Micropropagation is an alternative to produce orchid plants in large scale. However, this process presents losses during acclimatization. Exogenous proline use in vitro plant tissue culture can reduce the stress of the plant acclimatization phase. We aimed to verify the growth of orchids in different micropropagation systems with the addition of proline in the culture medium. Cattleya walkeriana plants were obtained from the germination of seeds in culture medium. Seeds were germinated in MS medium, added 20 g. L-1 of sucrose, solidified with 6 g. L-1 of agar and pH adjusted for 5,8. The cultures were incubated in a growth room with temperature of 24 ± 2 0C, under photoperiod of 16 h. After 5 months, 1-cm long seedlings were placed in a culture vessel according to the treatments, which were composed of two micropropagation systems (conventional and natural ventilation) and three proline concentrations (0, 1, and 2 g·L-1). The experiment was carried out in an entirely randomized design consisting of a 2 × 3 factorial, for a total of 6 treatments, each with 5 replicates. The natural ventilation system with the use of proline (1 g·L-1) promoted higher dry mass accumulation and better control of water loss by plants. |
publishDate |
2016 |
dc.date.none.fl_str_mv |
2016-06-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://seer.ufu.br/index.php/biosciencejournal/article/view/31368 10.14393/BJ-v32n3a2016-31368 |
url |
https://seer.ufu.br/index.php/biosciencejournal/article/view/31368 |
identifier_str_mv |
10.14393/BJ-v32n3a2016-31368 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
https://seer.ufu.br/index.php/biosciencejournal/article/view/31368/18304 |
dc.rights.driver.fl_str_mv |
https://creativecommons.org/licenses/by/4.0 info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by/4.0 |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.coverage.none.fl_str_mv |
Brazil; Contemporary |
dc.publisher.none.fl_str_mv |
EDUFU |
publisher.none.fl_str_mv |
EDUFU |
dc.source.none.fl_str_mv |
Bioscience Journal ; Vol. 32 No. 3 (2016): May/June; 619-626 Bioscience Journal ; v. 32 n. 3 (2016): May/June; 619-626 1981-3163 reponame:Bioscience journal (Online) instname:Universidade Federal de Uberlândia (UFU) instacron:UFU |
instname_str |
Universidade Federal de Uberlândia (UFU) |
instacron_str |
UFU |
institution |
UFU |
reponame_str |
Bioscience journal (Online) |
collection |
Bioscience journal (Online) |
repository.name.fl_str_mv |
Bioscience journal (Online) - Universidade Federal de Uberlândia (UFU) |
repository.mail.fl_str_mv |
biosciencej@ufu.br|| |
_version_ |
1797069075934871552 |