Doxycycline hyclate may damage testicular germinal epithelium in Wistar rats
Autor(a) principal: | |
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Data de Publicação: | 2023 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Bioscience journal (Online) |
Texto Completo: | https://seer.ufu.br/index.php/biosciencejournal/article/view/64399 |
Resumo: | Broad-spectrum antimicrobial doxycycline acts as an inhibitor of protein synthesis and it is widely used in the clinical treatment of various infections by microorganisms that are sensitive to the drug, as well as in animal feed. Its liposolubility guarantees its high tissue bioavailability, being associated with several biochemical changes in the organism and potentially adverse effects on reproduction. This study aims to evaluate the effects of the action of doxycycline on spermatogenesis to provide a complete analysis of the tubular and interstitial compartments and to identify possible changes in the testicular parenchyma. Adult male Wistar rats were divided into three groups: one control (water), and two treated with doxycycline at the doses of 10mg/kg and 30mg/kg, for 30 days. After euthanasia and sample processing, the following parameters were evaluated: a) tubular diameter and height of the seminiferous epithelium; b) volumetric proportions (%) and volumes (mL) of the components of the testicular parenchyma; c) counting testicular germ cell populations; d) evaluation of cell viability. The results of the comparative evaluation between the experimental groups demonstrated a significant increase in the diameter and area of the tubular lumen and a reduction in the count of spermatogonia in the experimental group that received doxycycline hyclate at a dose of 30mg/kg. In the same experimental group, an increase in the overall yield of spermatogenesis was found as a consequence of the increase in the mitotic index. |
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Doxycycline hyclate may damage testicular germinal epithelium in Wistar ratsHiclato de doxiciclina pode causar danos ao epitélio germinativo testicular em ratos WistarAntibioticsHistologyMale InfertilityMorphometryReproduction. Biological SciencesBroad-spectrum antimicrobial doxycycline acts as an inhibitor of protein synthesis and it is widely used in the clinical treatment of various infections by microorganisms that are sensitive to the drug, as well as in animal feed. Its liposolubility guarantees its high tissue bioavailability, being associated with several biochemical changes in the organism and potentially adverse effects on reproduction. This study aims to evaluate the effects of the action of doxycycline on spermatogenesis to provide a complete analysis of the tubular and interstitial compartments and to identify possible changes in the testicular parenchyma. Adult male Wistar rats were divided into three groups: one control (water), and two treated with doxycycline at the doses of 10mg/kg and 30mg/kg, for 30 days. After euthanasia and sample processing, the following parameters were evaluated: a) tubular diameter and height of the seminiferous epithelium; b) volumetric proportions (%) and volumes (mL) of the components of the testicular parenchyma; c) counting testicular germ cell populations; d) evaluation of cell viability. The results of the comparative evaluation between the experimental groups demonstrated a significant increase in the diameter and area of the tubular lumen and a reduction in the count of spermatogonia in the experimental group that received doxycycline hyclate at a dose of 30mg/kg. In the same experimental group, an increase in the overall yield of spermatogenesis was found as a consequence of the increase in the mitotic index.A doxiciclina antimicrobiana de amplo espectro atua como inibidor da síntese proteica e é amplamente utilizada no tratamento clínico de diversas infecções por microrganismos sensíveis ao fármaco, bem como na alimentação animal. Sua lipossolubilidade garante sua alta biodisponibilidade tecidual e seu uso está associado a diversas alterações bioquímicas no organismo e efeitos potencialmente adversos na reprodução. Este estudo tem como objetivo avaliar os efeitos da ação da doxiciclina na espermatogênese para fornecer uma análise completa dos compartimentos tubular e intersticial e identificar possíveis alterações no parênquima testicular. Ratos Wistar machos adultos foram divididos em três grupos: um controle (água) e dois tratados com doxiciclina nas doses de 10mg/kg e 30mg/kg, por 30 dias. Após a eutanásia e processamento das amostras, foram avaliados os seguintes parâmetros: a) diâmetro tubular e altura do epitélio seminífero; b) proporções volumétricas (%) e volumes (mL) dos componentes do parênquima testicular; c) contagem de populações de células germinativas testiculares; d) avaliação da viabilidade celular. Os resultados da avaliação comparativa entre os grupos experimentais demonstraram aumento significativo no diâmetro e área do lúmen tubular e redução na contagem de espermatogônias no grupo experimental que recebeu hidrato de doxiciclina na dose de 30mg/kg. No mesmo grupo experimental, foi encontrado um aumento no rendimento global da espermatogênese como consequência do aumento do índice mitótico.Universidade Federal de Uberlândia2023-03-10info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://seer.ufu.br/index.php/biosciencejournal/article/view/6439910.14393/BJ-v39n0a2023-64399Bioscience Journal ; Vol. 39 (2023): Continuous Publication; e39031Bioscience Journal ; v. 39 (2023): Continuous Publication; e390311981-3163reponame:Bioscience journal (Online)instname:Universidade Federal de Uberlândia (UFU)instacron:UFUenghttps://seer.ufu.br/index.php/biosciencejournal/article/view/64399/35674Brazil; Contemporary Copyright (c) 2023 João Vitor Lopes-Ferreira, Jullyana Costa Machado, Fernanda Carolina Riberio Dias, Luciana Schulthais Altoé, Reggiani Gonçalves Vilela, Sérgio Luis Pinto da Matta, Angelica de Oliveira Gomes, Marcos de Lucca Moreira Gomeshttps://creativecommons.org/licenses/by/4.0info:eu-repo/semantics/openAccessLopes-Ferreira, João VitorMachado, Jullyana Costa Dias, Fernanda Carolina Riberio Altoé, Luciana Schulthais Vilela, Reggiani Gonçalves Matta, Sérgio Luis Pinto da Gomes, Angelica de OliveiraGomes, Marcos de Lucca Moreira2024-01-31T19:16:18Zoai:ojs.www.seer.ufu.br:article/64399Revistahttps://seer.ufu.br/index.php/biosciencejournalPUBhttps://seer.ufu.br/index.php/biosciencejournal/oaibiosciencej@ufu.br||1981-31631516-3725opendoar:2024-01-31T19:16:18Bioscience journal (Online) - Universidade Federal de Uberlândia (UFU)false |
dc.title.none.fl_str_mv |
Doxycycline hyclate may damage testicular germinal epithelium in Wistar rats Hiclato de doxiciclina pode causar danos ao epitélio germinativo testicular em ratos Wistar |
title |
Doxycycline hyclate may damage testicular germinal epithelium in Wistar rats |
spellingShingle |
Doxycycline hyclate may damage testicular germinal epithelium in Wistar rats Lopes-Ferreira, João Vitor Antibiotics Histology Male Infertility Morphometry Reproduction. Biological Sciences |
title_short |
Doxycycline hyclate may damage testicular germinal epithelium in Wistar rats |
title_full |
Doxycycline hyclate may damage testicular germinal epithelium in Wistar rats |
title_fullStr |
Doxycycline hyclate may damage testicular germinal epithelium in Wistar rats |
title_full_unstemmed |
Doxycycline hyclate may damage testicular germinal epithelium in Wistar rats |
title_sort |
Doxycycline hyclate may damage testicular germinal epithelium in Wistar rats |
author |
Lopes-Ferreira, João Vitor |
author_facet |
Lopes-Ferreira, João Vitor Machado, Jullyana Costa Dias, Fernanda Carolina Riberio Altoé, Luciana Schulthais Vilela, Reggiani Gonçalves Matta, Sérgio Luis Pinto da Gomes, Angelica de Oliveira Gomes, Marcos de Lucca Moreira |
author_role |
author |
author2 |
Machado, Jullyana Costa Dias, Fernanda Carolina Riberio Altoé, Luciana Schulthais Vilela, Reggiani Gonçalves Matta, Sérgio Luis Pinto da Gomes, Angelica de Oliveira Gomes, Marcos de Lucca Moreira |
author2_role |
author author author author author author author |
dc.contributor.author.fl_str_mv |
Lopes-Ferreira, João Vitor Machado, Jullyana Costa Dias, Fernanda Carolina Riberio Altoé, Luciana Schulthais Vilela, Reggiani Gonçalves Matta, Sérgio Luis Pinto da Gomes, Angelica de Oliveira Gomes, Marcos de Lucca Moreira |
dc.subject.por.fl_str_mv |
Antibiotics Histology Male Infertility Morphometry Reproduction. Biological Sciences |
topic |
Antibiotics Histology Male Infertility Morphometry Reproduction. Biological Sciences |
description |
Broad-spectrum antimicrobial doxycycline acts as an inhibitor of protein synthesis and it is widely used in the clinical treatment of various infections by microorganisms that are sensitive to the drug, as well as in animal feed. Its liposolubility guarantees its high tissue bioavailability, being associated with several biochemical changes in the organism and potentially adverse effects on reproduction. This study aims to evaluate the effects of the action of doxycycline on spermatogenesis to provide a complete analysis of the tubular and interstitial compartments and to identify possible changes in the testicular parenchyma. Adult male Wistar rats were divided into three groups: one control (water), and two treated with doxycycline at the doses of 10mg/kg and 30mg/kg, for 30 days. After euthanasia and sample processing, the following parameters were evaluated: a) tubular diameter and height of the seminiferous epithelium; b) volumetric proportions (%) and volumes (mL) of the components of the testicular parenchyma; c) counting testicular germ cell populations; d) evaluation of cell viability. The results of the comparative evaluation between the experimental groups demonstrated a significant increase in the diameter and area of the tubular lumen and a reduction in the count of spermatogonia in the experimental group that received doxycycline hyclate at a dose of 30mg/kg. In the same experimental group, an increase in the overall yield of spermatogenesis was found as a consequence of the increase in the mitotic index. |
publishDate |
2023 |
dc.date.none.fl_str_mv |
2023-03-10 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://seer.ufu.br/index.php/biosciencejournal/article/view/64399 10.14393/BJ-v39n0a2023-64399 |
url |
https://seer.ufu.br/index.php/biosciencejournal/article/view/64399 |
identifier_str_mv |
10.14393/BJ-v39n0a2023-64399 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
https://seer.ufu.br/index.php/biosciencejournal/article/view/64399/35674 |
dc.rights.driver.fl_str_mv |
https://creativecommons.org/licenses/by/4.0 info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by/4.0 |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.coverage.none.fl_str_mv |
Brazil; Contemporary |
dc.publisher.none.fl_str_mv |
Universidade Federal de Uberlândia |
publisher.none.fl_str_mv |
Universidade Federal de Uberlândia |
dc.source.none.fl_str_mv |
Bioscience Journal ; Vol. 39 (2023): Continuous Publication; e39031 Bioscience Journal ; v. 39 (2023): Continuous Publication; e39031 1981-3163 reponame:Bioscience journal (Online) instname:Universidade Federal de Uberlândia (UFU) instacron:UFU |
instname_str |
Universidade Federal de Uberlândia (UFU) |
instacron_str |
UFU |
institution |
UFU |
reponame_str |
Bioscience journal (Online) |
collection |
Bioscience journal (Online) |
repository.name.fl_str_mv |
Bioscience journal (Online) - Universidade Federal de Uberlândia (UFU) |
repository.mail.fl_str_mv |
biosciencej@ufu.br|| |
_version_ |
1797069065226813440 |