Toxicity of Jatropha curcas L. latex in Allium cepa test
Autor(a) principal: | |
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Data de Publicação: | 2017 |
Outros Autores: | , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Bioscience journal (Online) |
Texto Completo: | https://seer.ufu.br/index.php/biosciencejournal/article/view/33835 |
Resumo: | The latex obtained from Jatropha curcas (physic nut) is used in traditional medicine to treat a variety of disturbs, including burns, hemorrhoids, ringworm and ulcers. Phytochemical analyses have shown that J. curcas latex contains natural compounds with therapeutic potential. In this study, the toxicity, cytotoxicity and genotoxicity effects of J. curcas latex on the root cells of Allium cepa were examined. Onion seeds and bulbs were exposed to seven different concentrations of latex and then the roots were submitted to macro and microscopic analyses. Water and sodium azide were used as negative and positive controls, respectively. The analysis of root growth showed that J. curcas crude latex or 50% diluted is highly toxic. Cytogenetic results showed that the mitotic index of the onion roots submitted to latex treatment decreased significantly compared to the negative control, which suggests that the latex is cytotoxic. High incidence of chromosome aberrations in the cells treated with J. curcas latex was observed too, indicating that the latex also presents genotoxic effect. The analyses presented in this report suggest the toxic, cytotoxic and genotoxic effects of J. curcas latex. Then, the indiscriminate use of J. curcas latex in folk medicine could bring risk to human health. |
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Toxicity of Jatropha curcas L. latex in Allium cepa test Toxicidade do látex de Jatropha curcas L. no modelo de Allium cepaantiproliferative effectgenotoxicitymedicinal plants and physic nut.The latex obtained from Jatropha curcas (physic nut) is used in traditional medicine to treat a variety of disturbs, including burns, hemorrhoids, ringworm and ulcers. Phytochemical analyses have shown that J. curcas latex contains natural compounds with therapeutic potential. In this study, the toxicity, cytotoxicity and genotoxicity effects of J. curcas latex on the root cells of Allium cepa were examined. Onion seeds and bulbs were exposed to seven different concentrations of latex and then the roots were submitted to macro and microscopic analyses. Water and sodium azide were used as negative and positive controls, respectively. The analysis of root growth showed that J. curcas crude latex or 50% diluted is highly toxic. Cytogenetic results showed that the mitotic index of the onion roots submitted to latex treatment decreased significantly compared to the negative control, which suggests that the latex is cytotoxic. High incidence of chromosome aberrations in the cells treated with J. curcas latex was observed too, indicating that the latex also presents genotoxic effect. The analyses presented in this report suggest the toxic, cytotoxic and genotoxic effects of J. curcas latex. Then, the indiscriminate use of J. curcas latex in folk medicine could bring risk to human health.O látex obtido de Jatropha curcas (pinhão manso) é usado na medicina tradicional para tratamento de diversos distúrbios, como queimaduras, hemorroida, micose e úlcera. Análises fitoquímicas apontaram que o látex de J. curcas contém compostos naturais com potencial terapêutico. Este estudo avaliou a toxicidade, citotoxicidade e genotoxicidade do látex de J. curcas em células da raiz de Allium cepa. Sementes e bulbos de cebola foram expostos á sete diferentes concentrações de látex e, então, as raízes foram submetidas a análises macro e microscópica. Água e azida sódica foram utilizadas como controle negativo e positivo, respectivamente. A análise do comprimento das raízes mostrou que o látex de J. curcas puro e diluído a 50% é altamente tóxico. O índice mitótico das raízes de cebola submetidas ao tratamento com o látex diminuiu significativamente comparado com o controle negativo, o que sugere que o látex é citotóxico. Uma alta incidência de aberrações cromossômicas em células tratadas com o látex de J. curcas também foi observada, indicando que o látex apresenta efeito genotóxico. Essa análise sugere que o látex de J. curcas possui efeitostóxico, citotóxico e genotóxico, sendo que o uso indiscriminado do látex de J. curcas na medicina popular pode trazer risco à saúde humana.EDUFU2017-09-21info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://seer.ufu.br/index.php/biosciencejournal/article/view/3383510.14393/BJ-v33n5a2017-33835Bioscience Journal ; Vol. 33 No. 5 (2017): Sept./Oct.; 1295-1304Bioscience Journal ; v. 33 n. 5 (2017): Sept./Oct.; 1295-13041981-3163reponame:Bioscience journal (Online)instname:Universidade Federal de Uberlândia (UFU)instacron:UFUenghttps://seer.ufu.br/index.php/biosciencejournal/article/view/33835/20834Brazil; ContemporaryCopyright (c) 2017 Angelina Luzia Ciappina, Fabrício Alves Ferreira, Illana Reis Pereira, Tiago Rodrigues Sousa, Fábio Santos Matos, Paulo Roberto Melo Reis, Pablo José Gonçalves; Elisa Flávia Luiz Cardoso Bailão; Luciane Madureira Almeidahttps://creativecommons.org/licenses/by/4.0info:eu-repo/semantics/openAccessCiappina, Angelina LuziaFerreira, Fabrício AlvesPereira, Illana ReisRodrigues Sousa, TiagoMatos, Fábio SantosMelo Reis, Paulo RobertoGonçalves, Pablo JoséBailão, Elisa Flávia Luiz CardosoAlmeida, Luciane Madureira2022-02-14T02:02:50Zoai:ojs.www.seer.ufu.br:article/33835Revistahttps://seer.ufu.br/index.php/biosciencejournalPUBhttps://seer.ufu.br/index.php/biosciencejournal/oaibiosciencej@ufu.br||1981-31631516-3725opendoar:2022-02-14T02:02:50Bioscience journal (Online) - Universidade Federal de Uberlândia (UFU)false |
dc.title.none.fl_str_mv |
Toxicity of Jatropha curcas L. latex in Allium cepa test Toxicidade do látex de Jatropha curcas L. no modelo de Allium cepa |
title |
Toxicity of Jatropha curcas L. latex in Allium cepa test |
spellingShingle |
Toxicity of Jatropha curcas L. latex in Allium cepa test Ciappina, Angelina Luzia antiproliferative effect genotoxicity medicinal plants and physic nut. |
title_short |
Toxicity of Jatropha curcas L. latex in Allium cepa test |
title_full |
Toxicity of Jatropha curcas L. latex in Allium cepa test |
title_fullStr |
Toxicity of Jatropha curcas L. latex in Allium cepa test |
title_full_unstemmed |
Toxicity of Jatropha curcas L. latex in Allium cepa test |
title_sort |
Toxicity of Jatropha curcas L. latex in Allium cepa test |
author |
Ciappina, Angelina Luzia |
author_facet |
Ciappina, Angelina Luzia Ferreira, Fabrício Alves Pereira, Illana Reis Rodrigues Sousa, Tiago Matos, Fábio Santos Melo Reis, Paulo Roberto Gonçalves, Pablo José Bailão, Elisa Flávia Luiz Cardoso Almeida, Luciane Madureira |
author_role |
author |
author2 |
Ferreira, Fabrício Alves Pereira, Illana Reis Rodrigues Sousa, Tiago Matos, Fábio Santos Melo Reis, Paulo Roberto Gonçalves, Pablo José Bailão, Elisa Flávia Luiz Cardoso Almeida, Luciane Madureira |
author2_role |
author author author author author author author author |
dc.contributor.author.fl_str_mv |
Ciappina, Angelina Luzia Ferreira, Fabrício Alves Pereira, Illana Reis Rodrigues Sousa, Tiago Matos, Fábio Santos Melo Reis, Paulo Roberto Gonçalves, Pablo José Bailão, Elisa Flávia Luiz Cardoso Almeida, Luciane Madureira |
dc.subject.por.fl_str_mv |
antiproliferative effect genotoxicity medicinal plants and physic nut. |
topic |
antiproliferative effect genotoxicity medicinal plants and physic nut. |
description |
The latex obtained from Jatropha curcas (physic nut) is used in traditional medicine to treat a variety of disturbs, including burns, hemorrhoids, ringworm and ulcers. Phytochemical analyses have shown that J. curcas latex contains natural compounds with therapeutic potential. In this study, the toxicity, cytotoxicity and genotoxicity effects of J. curcas latex on the root cells of Allium cepa were examined. Onion seeds and bulbs were exposed to seven different concentrations of latex and then the roots were submitted to macro and microscopic analyses. Water and sodium azide were used as negative and positive controls, respectively. The analysis of root growth showed that J. curcas crude latex or 50% diluted is highly toxic. Cytogenetic results showed that the mitotic index of the onion roots submitted to latex treatment decreased significantly compared to the negative control, which suggests that the latex is cytotoxic. High incidence of chromosome aberrations in the cells treated with J. curcas latex was observed too, indicating that the latex also presents genotoxic effect. The analyses presented in this report suggest the toxic, cytotoxic and genotoxic effects of J. curcas latex. Then, the indiscriminate use of J. curcas latex in folk medicine could bring risk to human health. |
publishDate |
2017 |
dc.date.none.fl_str_mv |
2017-09-21 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://seer.ufu.br/index.php/biosciencejournal/article/view/33835 10.14393/BJ-v33n5a2017-33835 |
url |
https://seer.ufu.br/index.php/biosciencejournal/article/view/33835 |
identifier_str_mv |
10.14393/BJ-v33n5a2017-33835 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
https://seer.ufu.br/index.php/biosciencejournal/article/view/33835/20834 |
dc.rights.driver.fl_str_mv |
https://creativecommons.org/licenses/by/4.0 info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by/4.0 |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.coverage.none.fl_str_mv |
Brazil; Contemporary |
dc.publisher.none.fl_str_mv |
EDUFU |
publisher.none.fl_str_mv |
EDUFU |
dc.source.none.fl_str_mv |
Bioscience Journal ; Vol. 33 No. 5 (2017): Sept./Oct.; 1295-1304 Bioscience Journal ; v. 33 n. 5 (2017): Sept./Oct.; 1295-1304 1981-3163 reponame:Bioscience journal (Online) instname:Universidade Federal de Uberlândia (UFU) instacron:UFU |
instname_str |
Universidade Federal de Uberlândia (UFU) |
instacron_str |
UFU |
institution |
UFU |
reponame_str |
Bioscience journal (Online) |
collection |
Bioscience journal (Online) |
repository.name.fl_str_mv |
Bioscience journal (Online) - Universidade Federal de Uberlândia (UFU) |
repository.mail.fl_str_mv |
biosciencej@ufu.br|| |
_version_ |
1797069076949893120 |