Enxerto osteocondral alógeno, associado à inoculação de células mononucleares autólogas da medula óssea no reparo do sulco troclear de coelhos

• Autor(a) principal: Souza, Luiz Augusto de
• Data de Publicação: 2009
• Tipo de documento: Dissertação
• Idioma: por
• Título da fonte: Repositório Institucional da UFU
• Texto Completo: https://repositorio.ufu.br/handle/123456789/12942
• topic: Cartilagem articular; Fêmur; Medula óssea; Osso; Terapia celular; Cirurgia veterinária; Articular cartilage; Femur; Bone-marrow; Bone; Cellular therapy; CNPQ::CIENCIAS AGRARIAS::MEDICINA VETERINARIA::CLINICA E CIRURGIA ANIMAL::CLINICA VETERINARIA

Twenty four rabbits were separate in control group (GC) and treated with stem cell group (GCT). Under general anesthesia a Rosenthal 16G needle was inserted into umeral tubercle to collect 2,0mL of bone-marrow. It was proceeded the isolation, the counting and the viability of the mesenchymal autologous cells. A lateral parapatelar incision was made over the left knee in skin and articular capsule. The patella was medially dislocated and a segment about 0,4cm x 1,0cm was removed in the throclear groove. In GC and GCT that local was filled by alogenic osteochondral graft conserved in glycerin. In GCT it was inject 2 x 106 mononuclear stem cells. On 45 and 90 days of postoperative, in 75% of GC animals the articular capsule presented thick and in 41,66% adhered to adjacent tissues. The graft was osteointegrated in 33,33% and 58,33% with bone alterations. In GCT the graft adhered into the receptor bed, in 91,66% degeneration process was not observed, and in 58,33% it was noted endochondral ossification of the subchondral bone with bone consolidation. Alogenic osteochondral grafts conserved in 98% glycerin in rabbits throclear groove repair, associated with intra-articular injection of autologous mononuclear cells, stimulate fibrocartilage and hyaline cartilage formation with integration of the graft into the receptor bed.