Determinação simultânea de hidroclorotiazida e metoprolol por eletroforese capilar com detecção condutométrica sem contato (CE-C4D)

• Autor(a) principal: Resende, Rayssa Borges
• Data de Publicação: 2019
• Tipo de documento: Trabalho de conclusão de curso
• Idioma: por
• Título da fonte: Repositório Institucional da UFU
• Texto Completo: https://repositorio.ufu.br/handle/123456789/27251
• topic: CE-C4D; Determinação simultânea; Amostras farmacêuticas; Controle de qualidade; Princípios ativos; Hidroclorotiazida; Metoprolol; CNPQ::CIENCIAS EXATAS E DA TERRA::QUIMICA::QUIMICA ANALITICA

In this work, capillary electrophoressis with contactless capacitive coupled conductivity detection technique (CE-C4D) was used for the development of a fast method of quality control of hydrochlorothiazide (HCT) e metoprolol (MET) pharmaceutical formulations. For simultaneous determination, the buffer (BGE) was composed by 10 mmol/L of H3BO3 adjusted with NaOH to pH = 9,0, with analysis time less than 80 seconds. The limits of detection (LOD) were 30 and 65 mol/L for HCT and MET, respectively. Calibration curves showed correlation coefficients greater than 0,993 and RSD values less than 5% (n=10). The metod presented recovery values of 95% and 102% for HCT and MET, respectively. Furthermore, the CE-C4D methods are simple, generates a minimum amount ofwaste (lower environmental impact), are low cost (equipment and maintenance) and has a high analytical frequency: 34 injetions h-1.