Funcionaliza??o de carbon dots com pept?deo antimicrobiano ecPis-4s

Detalhes bibliográficos
Autor(a) principal: Souza, Aniely Pereira de
Data de Publicação: 2021
Tipo de documento: Dissertação
Idioma: por
Título da fonte: Repositório Institucional da UFVJM
Texto Completo: http://acervo.ufvjm.edu.br/jspui/handle/1/2668
Resumo: O presente trabalho foi realizado com apoio da Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior - Brasil (CAPES) - C?digo de Financiamento 001.
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spelling Souza, Aniely Pereira deMesquita, Jo?o Paulo deMunhoz, Victor Hugo de OliveiraPereira, Fabiano VargasVerly, Rodrigo MoreiraUniversidade Federal dos Vales do Jequitinhonha e Mucuri (UFVJM)Mesquita, Jo?o Paulo de2021-10-04T19:13:03Z2021-10-04T19:13:03Z20212021-04-30SOUZA, Aniely Pereira de. Funcionaliza??o de carbon dots com pept?deo antimicrobiano ecPis-4s. 2021. 95 p. Disserta??o (Mestrado em Qu?mica) ? Programa de P?s-Gradua??o em Qu?mica, Universidade Federal dos Vales do Jequitinhonha e Mucuri, Diamantina, 2021.http://acervo.ufvjm.edu.br/jspui/handle/1/2668O presente trabalho foi realizado com apoio da Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior - Brasil (CAPES) - C?digo de Financiamento 001.Neste trabalho, os carbon dots (CDs) foram funcionalizados com o pept?deo ecPis-4s, com o objetivo de obter um (bio)nanomaterial com propriedades antioxidantes e antibacterianas, para futuras aplica??es relacionadas a intera??es pept?deo-membranas celular. Os CDs foram preparados a partir de rea??es de desidrata??o e oxida??o ?cida do biopol?mero celulose. A metodologia utilizada foi escolhida devido ? possibilidade de obten??o de nanopart?culas com alta concentra??o de grupos funcionais oxigenados, que ser?o ?teis para o liga??o do pept?deo antimicrobiano ecPis-4s nanopart?culas. A funcionaliza??o dos CDs foi realizada atrav?s da rea??o dos grupos carbonila e/ou hidroxila com o grupo amino ou am?nio do pept?deo por meio da ativa??o destes grupos usando 1-etil-3-(3-dimetilaminopropil) carbodiimida (EDAC) e acetato de etil-2-ciano-2-(hidroxiimino)(Oxyma pure?). Os materiais obtidos foram caracterizados com diferentes t?cnicas, incluindo Resson?ncia Magn?tica Nuclear de hidrog?nio (RMN ?H), espectroscopia na regi?o do infravermelho com transformada de Fourier (FTIR- do ingl?s Fourier Transform Infrared Spectroscopy) e potencial zeta. Ap?s a funcionaliza??o com o pept?deo, o potencial zeta das nanopart?culas de CDs passou de -50,5 mV para -23,4 mV, sugerindo o consumo de alguns grupos funcionais negativamente carregados, como os carbox?licos, em pH inferior a 6. Nos espectros no infravermelho e de RMN ?H dos CDs funcionalizados, ap?s purifica??o, p?de-se observar bandas caracter?sticas de ambos os compostos. Destaca-se, por exemplo, as absor??es em 1360 e 1050 cm-?, a primeira observada somente no espectro do CDs e a segunda bem definida no espectro do pept?deo. Em adi??o, observa-se a presen?a do grupo funcional amida por meio das absor??es em 1638 e 1560 cm-?. Al?m dessas observa??es, a funcionaliza??o pode ser indiretamente relacionada ao estreitamento da absor??o em 3340 cm-?(? O-H), o que sugere uma diminui??o das liga??es de hidrog?nio na amostra de CDs ap?s a rea??o. Se, por um lado, as nanoestruturas funcionalizadas n?o apresentaram atividade antioxidante, por outro, os resultados da atividade antimicrobiana in vitro, mostraram que as nanopart?culas funcionalizadas s?o capazes de inibir o crescimento bacteriano similarmente ao ecPis-4s para Staphylococcus aureus ( S. aureus), e Escherichia coli (E. coli), mostrando que elas podem inibir tanto o crescimento de bact?rias Gram-positivas quanto Gram?negativas. Este resultado est? diretamente relacionado ? conforma??o em ?-h?lice do pept?deo covalentemente ligado ? superf?cie dos CDs, como mostrado pelos resultados obtidos por dicro?smo circular em meio mim?tico de membranas.Submitted by Jos? Henrique Henrique (jose.neves@ufvjm.edu.br) on 2021-10-04T18:59:11Z No. of bitstreams: 2 license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) aniely_pereira_souza.pdf: 2476828 bytes, checksum: 272fe957f35be3acf8a5cd6cd8ee099b (MD5)Approved for entry into archive by Jos? Henrique Henrique (jose.neves@ufvjm.edu.br) on 2021-10-04T19:13:03Z (GMT) No. of bitstreams: 2 license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) aniely_pereira_souza.pdf: 2476828 bytes, checksum: 272fe957f35be3acf8a5cd6cd8ee099b (MD5)Made available in DSpace on 2021-10-04T19:13:03Z (GMT). No. of bitstreams: 2 license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) aniely_pereira_souza.pdf: 2476828 bytes, checksum: 272fe957f35be3acf8a5cd6cd8ee099b (MD5) Previous issue date: 2021Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior (CAPES)Disserta??o (Mestrado) ? Programa de P?s-Gradua??o em Qu?mica, Universidade Federal dos Vales do Jequitinhonha e Mucuri, 2021.Carbon dots (CDs) were functionalized with the ecPis-4s peptide, with the objective of obtaining a (bio)nanomaterial with antioxidant and antibacterial properties and future applications related with peptide-cell membrane interactions, such as biomarkers. The CDs were prepared from acid dehydration and oxidation reactions of the cellulose biopolymer. This methodology was chosen because, it allows the obtainment of allows the obtainment of nanoparticles with a high concentration of oxygenated functional groups, which are be useful for the binding of the antimicrobial peptide ecPis-4s on the nanoparticle. The functionalization of the CDs was performed through the reaction of the carboxyl and hidroxil groups (CDs) with the amino group of the peptide through the activation of carboxyl and/or OH groups using 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDAC) and ethyl-2- cyano-2- (hydroxyimino) acetate (Oxyme Pure?). The materials obtained were characterized through different techniques, including proton Nuclear Magnetic Resonance of hydrogen (?H NMR), Fourier Transform Infrared Spectroscopy (FTIR) and zeta potential. After the functionalization with the peptide, the zeta potential of the CD nanoparticles changed from - 50.5 mV to -23.4 mV, suggesting the consumption of some negatively charged functional groups, such as carboxy, at a pH below 6. In the FTIR and ?H NMR spectra of the functionalized CDs, after purification, characteristic bands of both compounds were observed. For example, the absorptions at 1360 and 1050 cm-?, the former observed only in the spectrum of the CDs and the latter well-defined in the spectrum of the peptide. The presence of the amide functional group is observed through absorptions in 1638 and 1560 cm-?. In addition to these observations, the functionalization can be also indirectly related to the narrowing of absorption band around by 3340 cm-?(? O-H), which suggests a decrease in hydrogen bonds in the sample of CDs after the reaction. If, on the one hand, the functionalized nanostructures did not show antioxidant activity, on the other, the results of the antimicrobial activity, in vitro, showed that the functionalized nanoparticles are capable of inhibiting bacterial growth similary to ecPis-4s for Staphylococcus aureus (S. aureus), and Escherichia coli (E. coli), showing that they can inhibit growth with both Gram-positive and Gram-negative bacteria. This outcome is directly related to the ?-helix conformation of the peptide covalently attached to the surface of the CDs, as shown by the results obtained with circular dichroism in a membrane mimetic medium.porUFVJMA concess?o da licen?a deste item refere-se ao ? termo de autoriza??o impresso assinado pelo autor, assim como na licen?a Creative Commons, com as seguintes condi??es: Na qualidade de titular dos direitos de autor da publica??o, autorizo a Universidade Federal dos Vales do Jequitinhonha e Mucuri e o IBICT a disponibilizar por meio de seus reposit?rios, sem ressarcimento dos direitos autorais, de acordo com a Lei n? 9610/98, o texto integral da obra disponibilizada, conforme permiss?es assinaladas, para fins de leitura, impress?o e/ou download, a t?tulo de divulga??o da produ??o cient?fica brasileira, e preserva??o, a partir desta data.info:eu-repo/semantics/openAccessFuncionaliza??o de carbon dots com pept?deo antimicrobiano ecPis-4sinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisCarbon dotsCovalent functionalizationAntimicrobial peptideAntioxidantFuncionaliza??o covalentePept?deo antimicrobianoecPis-4sAntioxidantereponame:Repositório Institucional da UFVJMinstname:Universidade Federal dos Vales do Jequitinhonha e Mucuri (UFVJM)instacron:UFVJMTEXTaniely_pereira_souza.pdf.txtaniely_pereira_souza.pdf.txtExtracted texttext/plain173131http://acervo.ufvjm.edu.br/jspui/bitstream/1/2668/6/aniely_pereira_souza.pdf.txtc2085f8239e7baebc48c9234efa42611MD56LICENSElicense.txtlicense.txttext/plain; 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dc.title.pt_BR.fl_str_mv Funcionaliza??o de carbon dots com pept?deo antimicrobiano ecPis-4s
title Funcionaliza??o de carbon dots com pept?deo antimicrobiano ecPis-4s
spellingShingle Funcionaliza??o de carbon dots com pept?deo antimicrobiano ecPis-4s
Souza, Aniely Pereira de
Carbon dots
Covalent functionalization
Antimicrobial peptide
Antioxidant
Funcionaliza??o covalente
Pept?deo antimicrobiano
ecPis-4s
Antioxidante
title_short Funcionaliza??o de carbon dots com pept?deo antimicrobiano ecPis-4s
title_full Funcionaliza??o de carbon dots com pept?deo antimicrobiano ecPis-4s
title_fullStr Funcionaliza??o de carbon dots com pept?deo antimicrobiano ecPis-4s
title_full_unstemmed Funcionaliza??o de carbon dots com pept?deo antimicrobiano ecPis-4s
title_sort Funcionaliza??o de carbon dots com pept?deo antimicrobiano ecPis-4s
author Souza, Aniely Pereira de
author_facet Souza, Aniely Pereira de
author_role author
dc.contributor.referee.none.fl_str_mv Mesquita, Jo?o Paulo de
Munhoz, Victor Hugo de Oliveira
Pereira, Fabiano Vargas
dc.contributor.advisorco.none.fl_str_mv Verly, Rodrigo Moreira
dc.contributor.institution.pt_BR.fl_str_mv Universidade Federal dos Vales do Jequitinhonha e Mucuri (UFVJM)
dc.contributor.author.fl_str_mv Souza, Aniely Pereira de
dc.contributor.advisor1.fl_str_mv Mesquita, Jo?o Paulo de
contributor_str_mv Mesquita, Jo?o Paulo de
dc.subject.keyword.en.fl_str_mv Carbon dots
Covalent functionalization
Antimicrobial peptide
Antioxidant
topic Carbon dots
Covalent functionalization
Antimicrobial peptide
Antioxidant
Funcionaliza??o covalente
Pept?deo antimicrobiano
ecPis-4s
Antioxidante
dc.subject.keyword.pt_BR.fl_str_mv Funcionaliza??o covalente
Pept?deo antimicrobiano
ecPis-4s
Antioxidante
description O presente trabalho foi realizado com apoio da Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior - Brasil (CAPES) - C?digo de Financiamento 001.
publishDate 2021
dc.date.submitted.none.fl_str_mv 2021-04-30
dc.date.accessioned.fl_str_mv 2021-10-04T19:13:03Z
dc.date.available.fl_str_mv 2021-10-04T19:13:03Z
dc.date.issued.fl_str_mv 2021
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/masterThesis
format masterThesis
status_str publishedVersion
dc.identifier.citation.fl_str_mv SOUZA, Aniely Pereira de. Funcionaliza??o de carbon dots com pept?deo antimicrobiano ecPis-4s. 2021. 95 p. Disserta??o (Mestrado em Qu?mica) ? Programa de P?s-Gradua??o em Qu?mica, Universidade Federal dos Vales do Jequitinhonha e Mucuri, Diamantina, 2021.
dc.identifier.uri.fl_str_mv http://acervo.ufvjm.edu.br/jspui/handle/1/2668
identifier_str_mv SOUZA, Aniely Pereira de. Funcionaliza??o de carbon dots com pept?deo antimicrobiano ecPis-4s. 2021. 95 p. Disserta??o (Mestrado em Qu?mica) ? Programa de P?s-Gradua??o em Qu?mica, Universidade Federal dos Vales do Jequitinhonha e Mucuri, Diamantina, 2021.
url http://acervo.ufvjm.edu.br/jspui/handle/1/2668
dc.language.iso.fl_str_mv por
language por
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv UFVJM
publisher.none.fl_str_mv UFVJM
dc.source.none.fl_str_mv reponame:Repositório Institucional da UFVJM
instname:Universidade Federal dos Vales do Jequitinhonha e Mucuri (UFVJM)
instacron:UFVJM
instname_str Universidade Federal dos Vales do Jequitinhonha e Mucuri (UFVJM)
instacron_str UFVJM
institution UFVJM
reponame_str Repositório Institucional da UFVJM
collection Repositório Institucional da UFVJM
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