Isolamento de micro-organismos celulol?ticos em ?reas de floresta de eucaliptos da regi?o de Itamarandiba ? MG
Autor(a) principal: | |
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Data de Publicação: | 2018 |
Tipo de documento: | Dissertação |
Idioma: | por |
Título da fonte: | Repositório Institucional da UFVJM |
Texto Completo: | http://acervo.ufvjm.edu.br/jspui/handle/1/1803 |
Resumo: | ?rea de concentra??o: Processos qu?micos e bioqu?micos. |
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Cruz, Gizelly Gomes daLaia, Marcelo Luiz deGuimar?es, Amanda Gon?alvesSantos, Jos? Barbosa dosUniversidade Federal dos Vales do Jequitinhonha e Mucuri (UFVJM)Laia, Marcelo Luiz de2018-10-26T13:55:45Z2018-10-26T13:55:45Z20182018-03-27CRUZ, Gizelly Gomes da. Isolamento de micro-organismos celulol?ticos em ?reas de floresta de eucaliptos da regi?o de Itamarandiba ? MG. 2018. 83 p. Disserta??o (Mestrado) ? Programa de P?s-gradua??o em Biocombust?veis, Universidade Federal dos Vales do Jequitinhonha e Mucuri, Diamantina, 2018.http://acervo.ufvjm.edu.br/jspui/handle/1/1803?rea de concentra??o: Processos qu?micos e bioqu?micos.A introdu??o de biomassa na cadeia produtiva de biocombust?veis como fonte renov?vel, vem aumentando gradativamente, para reduzir os impactos ambientais e a depend?ncia do carv?o mineral e do petr?leo. No entanto, para garantir a viabilidade econ?mica do processo de convers?o de biomassa em combust?vel, ? fundamental reduzir o custo da etapa de hidr?lise da biomassa. O uso de micro-organismos para a hidr?lise e para a convers?o de celulose em etanol tem chamado a aten??o de empresas que atuam no desenvolvimento de enzimas, em especial o complexo celulase. Diante disso, o presente trabalho teve como objetivo selecionar micro-organismos potenciais produtores de celulases. Para isso, amostras de solos e serapilheira sob floresta de eucalipto em diferentes idades de plantio foram coletadas e submetidas ao isolamento de micro-organismos em meio de cultura seletivo. Escolheu-se solos em ?reas de cultivo de eucalipto devido o objetivo ser selecionar micro-organismos que atuem, especificamente, em madeira de eucalipto. Foram isolados 870 micro-organismos para an?lises com o m?todo de descolora??o de vermelho congo. 336 fungos filamentosos, 8 bact?rias e 3 leveduras foram selecionados com capacidade de sintetizar enzimas celulol?ticas. Destes, 163 foram avaliados como bons produtores de celulases, devido apresentarem ?ndices enzim?ticos superior a 2,5. Destaca-se que os isolados 25?50, 1?74 e 1?75 apresentaram elevados ?ndices enzim?ticos: 24,0; 22,0 e 20,0, respectivamente. A partir desses dados, esses isolados podem ser estudados molecularmente e o gene envolvido na produ??o dessa enzima ser caracterizado e clonado. Os resultados obtidos no presente trabalho mostram a import?ncia da prospec??o de novas linhagens de micro-organismos produtores de celulases. De maneira geral, pode-se concluir que ? poss?vel a utiliza??o de t?cnicas de screening para selecionar linhagens de um extenso banco de isolados. A metodologia de isolamento e cultivo proporcionou a obten??o de 348 isolados celulol?ticos, sendo poss?vel selecionar v?rios deles pelas altas produ??es enzim?ticas observadas durante as an?lises.Submitted by Jos? Henrique Henrique (jose.neves@ufvjm.edu.br) on 2018-08-23T15:30:11Z No. of bitstreams: 2 license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) gizelly_gomes_cruz.pdf: 2210427 bytes, checksum: 0a750e3bc57b1680a56b4243d929b7f4 (MD5)Approved for entry into archive by Rodrigo Martins Cruz (rodrigo.cruz@ufvjm.edu.br) on 2018-10-26T13:55:45Z (GMT) No. of bitstreams: 2 license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) gizelly_gomes_cruz.pdf: 2210427 bytes, checksum: 0a750e3bc57b1680a56b4243d929b7f4 (MD5)Made available in DSpace on 2018-10-26T13:55:45Z (GMT). No. of bitstreams: 2 license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) gizelly_gomes_cruz.pdf: 2210427 bytes, checksum: 0a750e3bc57b1680a56b4243d929b7f4 (MD5) Previous issue date: 2018Disserta??o (Mestrado) ? Programa de P?s-gradua??o em Biocombust?veis, Universidade Federal dos Vales do Jequitinhonha e Mucuri, 2018.The introduction of biomass in the biofuels production chain as a renewable source has been gradually increasing to reduce environmental impacts and dependence on coal and oil. However, to ensure the economic viability of the biomass conversion process into fuel, it is critical to reduce the cost of the biomass hydrolysis step. The use of microorganisms for the hydrolysis and conversion of cellulose into ethanol has attracted the attention of companies involved in the development of enzymes, especially the cellulase complex. Therefore, the present work aimed to select potential micro-organisms producing cellulases. For this, samples of soils and litter under eucalyptus forest at different planting ages were collected and submitted to the isolation of microorganisms in selective culture medium. Soils were selected in areas of eucalyptus cultivation due to the objective being to select microorganisms that specifically act on eucalyptus wood. 870 microorganisms were isolated for analysis with the congo red discoloration method. 336 filamentous fungi, 8 bacteria and 3 yeasts were selected with the ability to synthesize cellulolytic enzymes. Of these, 163 were evaluated as good producers of cellulases, due to their enzymatic indexes higher than 2.5. It is noteworthy that isolates 25-50, 1-74 and 1-75 showed high enzymatic indices: 24.0; 22.0 and 20.0, respectively. From these data, these isolates can be molecularly studied and the gene involved in the production of that enzyme is characterized and cloned. The results obtained in the present work show the importance of the prospection of new lines of microorganisms producing cellulases. In general, it can be concluded that it is possible to use screening techniques to select strains from an extensive database of isolates. The isolation and cultivation methodology allowed the obtaining of 348 cellulolytic isolates, being possible to select several of them due to the high enzymatic productions observed during the analyzes.porUFVJMA concess?o da licen?a deste item refere-se ao ? termo de autoriza??o impresso assinado pelo autor, assim como na licen?a Creative Commons, com as seguintes condi??es: Na qualidade de titular dos direitos de autor da publica??o, autorizo a Universidade Federal dos Vales do Jequitinhonha e Mucuri e o IBICT a disponibilizar por meio de seus reposit?rios, sem ressarcimento dos direitos autorais, de acordo com a Lei n? 9610/98, o texto integral da obra disponibilizada, conforme permiss?es assinaladas, para fins de leitura, impress?o e/ou download, a t?tulo de divulga??o da produ??o cient?fica brasileira, e preserva??o, a partir desta data.info:eu-repo/semantics/openAccessIsolamento de micro-organismos celulol?ticos em ?reas de floresta de eucaliptos da regi?o de Itamarandiba ? 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dc.title.pt_BR.fl_str_mv |
Isolamento de micro-organismos celulol?ticos em ?reas de floresta de eucaliptos da regi?o de Itamarandiba ? MG |
title |
Isolamento de micro-organismos celulol?ticos em ?reas de floresta de eucaliptos da regi?o de Itamarandiba ? MG |
spellingShingle |
Isolamento de micro-organismos celulol?ticos em ?reas de floresta de eucaliptos da regi?o de Itamarandiba ? MG Cruz, Gizelly Gomes da Biocombust?veis Bioetanol Eucalipto Micro-organismos Biofuels Bioethanol Eucalyptus Microorganisms |
title_short |
Isolamento de micro-organismos celulol?ticos em ?reas de floresta de eucaliptos da regi?o de Itamarandiba ? MG |
title_full |
Isolamento de micro-organismos celulol?ticos em ?reas de floresta de eucaliptos da regi?o de Itamarandiba ? MG |
title_fullStr |
Isolamento de micro-organismos celulol?ticos em ?reas de floresta de eucaliptos da regi?o de Itamarandiba ? MG |
title_full_unstemmed |
Isolamento de micro-organismos celulol?ticos em ?reas de floresta de eucaliptos da regi?o de Itamarandiba ? MG |
title_sort |
Isolamento de micro-organismos celulol?ticos em ?reas de floresta de eucaliptos da regi?o de Itamarandiba ? MG |
author |
Cruz, Gizelly Gomes da |
author_facet |
Cruz, Gizelly Gomes da |
author_role |
author |
dc.contributor.references.none.fl_str_mv |
Laia, Marcelo Luiz de Guimar?es, Amanda Gon?alves Santos, Jos? Barbosa dos |
dc.contributor.institution.pt_BR.fl_str_mv |
Universidade Federal dos Vales do Jequitinhonha e Mucuri (UFVJM) |
dc.contributor.author.fl_str_mv |
Cruz, Gizelly Gomes da |
dc.contributor.advisor1.fl_str_mv |
Laia, Marcelo Luiz de |
contributor_str_mv |
Laia, Marcelo Luiz de |
dc.subject.keyword.pt_BR.fl_str_mv |
Biocombust?veis Bioetanol Eucalipto Micro-organismos |
topic |
Biocombust?veis Bioetanol Eucalipto Micro-organismos Biofuels Bioethanol Eucalyptus Microorganisms |
dc.subject.keyword.en.fl_str_mv |
Biofuels Bioethanol Eucalyptus Microorganisms |
description |
?rea de concentra??o: Processos qu?micos e bioqu?micos. |
publishDate |
2018 |
dc.date.submitted.none.fl_str_mv |
2018-03-27 |
dc.date.accessioned.fl_str_mv |
2018-10-26T13:55:45Z |
dc.date.available.fl_str_mv |
2018-10-26T13:55:45Z |
dc.date.issued.fl_str_mv |
2018 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/masterThesis |
format |
masterThesis |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
CRUZ, Gizelly Gomes da. Isolamento de micro-organismos celulol?ticos em ?reas de floresta de eucaliptos da regi?o de Itamarandiba ? MG. 2018. 83 p. Disserta??o (Mestrado) ? Programa de P?s-gradua??o em Biocombust?veis, Universidade Federal dos Vales do Jequitinhonha e Mucuri, Diamantina, 2018. |
dc.identifier.uri.fl_str_mv |
http://acervo.ufvjm.edu.br/jspui/handle/1/1803 |
identifier_str_mv |
CRUZ, Gizelly Gomes da. Isolamento de micro-organismos celulol?ticos em ?reas de floresta de eucaliptos da regi?o de Itamarandiba ? MG. 2018. 83 p. Disserta??o (Mestrado) ? Programa de P?s-gradua??o em Biocombust?veis, Universidade Federal dos Vales do Jequitinhonha e Mucuri, Diamantina, 2018. |
url |
http://acervo.ufvjm.edu.br/jspui/handle/1/1803 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.publisher.none.fl_str_mv |
UFVJM |
publisher.none.fl_str_mv |
UFVJM |
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reponame:Repositório Institucional da UFVJM instname:Universidade Federal dos Vales do Jequitinhonha e Mucuri (UFVJM) instacron:UFVJM |
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Universidade Federal dos Vales do Jequitinhonha e Mucuri (UFVJM) |
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UFVJM |
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UFVJM |
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Repositório Institucional da UFVJM |
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Repositório Institucional da UFVJM |
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