Expressão diferencial de genes em células foliculares de suínos
Autor(a) principal: | |
---|---|
Data de Publicação: | 2007 |
Tipo de documento: | Dissertação |
Idioma: | por |
Título da fonte: | LOCUS Repositório Institucional da UFV |
Texto Completo: | http://locus.ufv.br/handle/123456789/4821 |
Resumo: | Among the productive traits, the number of the piglets is essential for success in swine production. The ovulation rate is one of the key factors that determine the litter size in pigs and the study of gene expression in the ovary follicles, more precisely in the follicular cells, might cause a big advance in the comprehension of this characteristic. Thus, the objective in this work was to identify the differential expression of the STAR (Steroidogenic Acute Regulator-STAR), GATA (GATA binding protein 4), PGF2α (Prostaglandin F2α), P4R (Progesterone Receptor), FSHR (Follicle Stimulating Hormone Receptor) and CYP19 (Cytochrome Aromatase P450) genes in follicular cells, colleted during the follicular phase in the estrous cycle of three sows with high and three sows with low litter size which comes from a tricross of the Landrace, Large White and Pietrain breeds, throughout the semi quantitative real time PCR (qPCR). In the hyperprolific sows, analyzed in this study, the average of the number of the piglets were 8,51 and for the hypoprolific sows the average was 12,03. For examination if any one related genes above were affecting the ovulation rate in the prolificity rate in the animals studied, the total RNA of the follicular liquid was extracted and than was made two pools with the total RNA of the hypo and hyperprolific sows groups. The total RNA of each pool was used for the first strand cDNA synthesis. The PCR reactions were accomplished using as an endogenous control the β-Actina gene. The primers were generated by sequences in the Pig ESTs data base. The major expression difference was observed for P4R gene, who express 7,73 turns mostly in the animals with low prolificity. The PGF2α gene displayed differential expression of 2,84 times as a large in the hypoprolific animals. The STAR gene expression, that codes for the protein regulating hormone steroids synthesis, was 2.32 turns mostly in the animals with low prolificity sows. The GATA gene expression was 2.31 times as a large in the hypoprolific sows. This gene codes for the GATA binding protein 4 belonging a transcription group factors that are responsible for too many gene expression and diverse cell differentiation types. The CYP19 gene expression taken the enzyme production that is the key factor in the estrogens biosynthesis, the Cytochrome Aromatase P450 (P450aro), for this gene was observed the relative expression of the 2.30 turns mostly in the sows with low prolificity. The FSHR gene expression hasn t achieved the threshold difference established in this study (1.5 times).The STAR, GATA, PGF2α, FSHR, P4R and CYP19 gene expression in the follicular cells has enabled identify difference of expression between sows with low and high prolificity, where the largest difference of expression in relation to the studied genes was verify in the hypoprolific sows, except for the FSHR gene that wasn t considerate differentially express in this work. With views the best agreement about the reproductive phenotypes in swine, it s necessary yet, studies to examine the expression in these and other genes related to female reproductive cycle, in as much as gene expression is dependent, in the midst of another factors, the stage of development of the tissue and the animal age. |
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Miranda, Cristiana Libardihttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4718446T8Guimarães, Marta Fonseca Martinshttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4790685D6Lopes, Paulo Sáviohttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4783377H1Guimarães, Simone Eliza Facionihttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4782526Y2Guimarães, José Domingoshttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4782270U6Iguma, Lilian Tamyhttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4707954P22015-03-26T13:42:36Z2007-10-112015-03-26T13:42:36Z2007-07-13MIRANDA, Cristiana Libardi. Gene expression diferentialy in follicular cells in swine. 2007. 56 f. Dissertação (Mestrado em Genética animal; Genética molecular e de microrganismos; Genética quantitativa; Genética vegetal; Me) - Universidade Federal de Viçosa, Viçosa, 2007.http://locus.ufv.br/handle/123456789/4821Among the productive traits, the number of the piglets is essential for success in swine production. The ovulation rate is one of the key factors that determine the litter size in pigs and the study of gene expression in the ovary follicles, more precisely in the follicular cells, might cause a big advance in the comprehension of this characteristic. Thus, the objective in this work was to identify the differential expression of the STAR (Steroidogenic Acute Regulator-STAR), GATA (GATA binding protein 4), PGF2α (Prostaglandin F2α), P4R (Progesterone Receptor), FSHR (Follicle Stimulating Hormone Receptor) and CYP19 (Cytochrome Aromatase P450) genes in follicular cells, colleted during the follicular phase in the estrous cycle of three sows with high and three sows with low litter size which comes from a tricross of the Landrace, Large White and Pietrain breeds, throughout the semi quantitative real time PCR (qPCR). In the hyperprolific sows, analyzed in this study, the average of the number of the piglets were 8,51 and for the hypoprolific sows the average was 12,03. For examination if any one related genes above were affecting the ovulation rate in the prolificity rate in the animals studied, the total RNA of the follicular liquid was extracted and than was made two pools with the total RNA of the hypo and hyperprolific sows groups. The total RNA of each pool was used for the first strand cDNA synthesis. The PCR reactions were accomplished using as an endogenous control the β-Actina gene. The primers were generated by sequences in the Pig ESTs data base. The major expression difference was observed for P4R gene, who express 7,73 turns mostly in the animals with low prolificity. The PGF2α gene displayed differential expression of 2,84 times as a large in the hypoprolific animals. The STAR gene expression, that codes for the protein regulating hormone steroids synthesis, was 2.32 turns mostly in the animals with low prolificity sows. The GATA gene expression was 2.31 times as a large in the hypoprolific sows. This gene codes for the GATA binding protein 4 belonging a transcription group factors that are responsible for too many gene expression and diverse cell differentiation types. The CYP19 gene expression taken the enzyme production that is the key factor in the estrogens biosynthesis, the Cytochrome Aromatase P450 (P450aro), for this gene was observed the relative expression of the 2.30 turns mostly in the sows with low prolificity. The FSHR gene expression hasn t achieved the threshold difference established in this study (1.5 times).The STAR, GATA, PGF2α, FSHR, P4R and CYP19 gene expression in the follicular cells has enabled identify difference of expression between sows with low and high prolificity, where the largest difference of expression in relation to the studied genes was verify in the hypoprolific sows, except for the FSHR gene that wasn t considerate differentially express in this work. With views the best agreement about the reproductive phenotypes in swine, it s necessary yet, studies to examine the expression in these and other genes related to female reproductive cycle, in as much as gene expression is dependent, in the midst of another factors, the stage of development of the tissue and the animal age.Dentre as características produtivas, o número de leitões é fundamental para o sucesso na produção de suínos. A taxa de ovulação é um dos principais fatores que determinam o tamanho da leitegada. Deste modo, o estudo da expressão gênica nos folículos ovarianos, mais precisamente nas células foliculares, pode causar grande avanço no entendimento desta característica. Portanto o presente trabalho foi realizado com o objetivo de identificar a expressão diferencial dos genes STAR (Proteína esteroidogênica regulatória aguda), GATA (Proteína de ligação GATA-4), PGF2α (Prostaglandina F2α), P4R (Receptor de progesterona 4), FSHR (Receptor do hormônio folículo estimulante) e CYP19 (Citocromo aromatase P450) em células foliculares, coletadas durante a fase folicular do ciclo estral de três fêmeas suínas de alta e três de baixa prolificidade, provenientes de um tricross das raças Landrace, Large White e Pietrain, por meio da metodologia de PCR quantitativo em tempo real (qPCR). Nas fêmeas com alta prolificidade, utilizadas neste estudo, a média do número de leitões por leitegada foi 8,51 enquanto para as fêmeas com baixa prolificidade foi de 12,03. Para examinar se algum dos genes relacionados acima afetava as taxas de prolicifidade nos animais deste estudo, o RNA total do líquido folicular foi extraído e, em seguida, fez-se dois pools com o RNA total, sendo um do grupo de fêmeas com alta prolificidade e o outro do grupo com baixa prolificidade. O RNA total de cada um dos pools foi usado na confecção da primeira fita de cDNA. As reações de qPCR foram realizadas usando-se o gene β-Actina como controle endógeno. Os primers foram gerados a partir de seqüências obtidas nos bancos de ESTs de suínos. A maior diferença de expressão foi observada para o gene P4R, que foi expresso 7,73 vezes a mais nos animais com baixa prolificidade. O gene PGF2α apresentou expressão diferencial de 2,84 vezes a mais nas fêmeas hipoprolíficas. A expressão do gene STAR, que codifica para uma proteína reguladora da síntese de hormônios esteróides, foi 2,32 vezes maior nos animais com baixa prolificidade. A expressão do gene GATA foi 2,31 vezes maior nas fêmeas hipoprolíficas. Esse gene codifica para a proteína de ligação GATA-4, pertencente a um grupo de fatores de transcrição responsáveis pela expressão de vários genes e a diferenciação de diversos tipos celulares. A expressão do CYP19 leva à produção de uma enzima chave na biossíntese de estrogênio, a Citocromo aromatase P450 (P450aro), sendo que, para este gene, foi observada uma expressão relativa de 2,30 vezes a mais nas fêmeas de baixa prolificidade. O gene FSHR não atingiu o limiar de diferença de expressão, estabelecido neste estudo (1,5 vezes). A expressão dos genes STAR, GATA, PGF2α, FSHR, P4R e CYP19 nas células foliculares possibilitou a identificação de diferenças de expressão entre as fêmeas com alta e baixa prolificidade, sendo que a maior diferença de expressão para os genes estudados foi verificada nos animais de baixa prolificidade, exceto para o gene FSHR, que não foi considerado diferencialmente expresso nesse trabalho. Com vistas ao melhor entendimento dos fenótipos reprodutivos em suínos, é necessário que estudos sejam conduzifos no sentido de examinar a expressão desses e de outros genes relacionados, durante todo do ciclo reprodutivo das fêmeas, pois, a expressão de um gene depende, dentre outros fatores, do estádio do desenvolvimento do tecido e da idade do animal.Coordenação de Aperfeiçoamento de Pessoal de Nível Superiorapplication/pdfporUniversidade Federal de ViçosaMestrado em Genética e MelhoramentoUFVBRGenética animal; Genética molecular e de microrganismos; Genética quantitativa; Genética vegetal; MePCR quantitativo em tempo realExpressão gênicaProlificidadeQuantitative real time PCRGene expressionProlificityCNPQ::CIENCIAS AGRARIAS::ZOOTECNIA::GENETICA E MELHORAMENTO DOS ANIMAIS DOMESTICOSExpressão diferencial de genes em células foliculares de suínosGene expression diferentialy in follicular cells in swineinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisinfo:eu-repo/semantics/openAccessreponame:LOCUS Repositório Institucional da UFVinstname:Universidade Federal de Viçosa (UFV)instacron:UFVORIGINALtexto completo.pdfapplication/pdf209546https://locus.ufv.br//bitstream/123456789/4821/1/texto%20completo.pdfd5ff785c114b2b115999b7a4b5ade9bdMD51TEXTtexto completo.pdf.txttexto completo.pdf.txtExtracted texttext/plain95579https://locus.ufv.br//bitstream/123456789/4821/2/texto%20completo.pdf.txt2b8a6b2833ec7b7f628cf6c8bc9015f2MD52THUMBNAILtexto completo.pdf.jpgtexto completo.pdf.jpgIM Thumbnailimage/jpeg3617https://locus.ufv.br//bitstream/123456789/4821/3/texto%20completo.pdf.jpg2f15405ce1844436f7253f589fbb98e7MD53123456789/48212016-04-10 23:16:20.039oai:locus.ufv.br:123456789/4821Repositório InstitucionalPUBhttps://www.locus.ufv.br/oai/requestfabiojreis@ufv.bropendoar:21452016-04-11T02:16:20LOCUS Repositório Institucional da UFV - Universidade Federal de Viçosa (UFV)false |
dc.title.por.fl_str_mv |
Expressão diferencial de genes em células foliculares de suínos |
dc.title.alternative.eng.fl_str_mv |
Gene expression diferentialy in follicular cells in swine |
title |
Expressão diferencial de genes em células foliculares de suínos |
spellingShingle |
Expressão diferencial de genes em células foliculares de suínos Miranda, Cristiana Libardi PCR quantitativo em tempo real Expressão gênica Prolificidade Quantitative real time PCR Gene expression Prolificity CNPQ::CIENCIAS AGRARIAS::ZOOTECNIA::GENETICA E MELHORAMENTO DOS ANIMAIS DOMESTICOS |
title_short |
Expressão diferencial de genes em células foliculares de suínos |
title_full |
Expressão diferencial de genes em células foliculares de suínos |
title_fullStr |
Expressão diferencial de genes em células foliculares de suínos |
title_full_unstemmed |
Expressão diferencial de genes em células foliculares de suínos |
title_sort |
Expressão diferencial de genes em células foliculares de suínos |
author |
Miranda, Cristiana Libardi |
author_facet |
Miranda, Cristiana Libardi |
author_role |
author |
dc.contributor.authorLattes.por.fl_str_mv |
http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4718446T8 |
dc.contributor.author.fl_str_mv |
Miranda, Cristiana Libardi |
dc.contributor.advisor-co1.fl_str_mv |
Guimarães, Marta Fonseca Martins |
dc.contributor.advisor-co1Lattes.fl_str_mv |
http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4790685D6 |
dc.contributor.advisor-co2.fl_str_mv |
Lopes, Paulo Sávio |
dc.contributor.advisor-co2Lattes.fl_str_mv |
http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4783377H1 |
dc.contributor.advisor1.fl_str_mv |
Guimarães, Simone Eliza Facioni |
dc.contributor.advisor1Lattes.fl_str_mv |
http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4782526Y2 |
dc.contributor.referee1.fl_str_mv |
Guimarães, José Domingos |
dc.contributor.referee1Lattes.fl_str_mv |
http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4782270U6 |
dc.contributor.referee2.fl_str_mv |
Iguma, Lilian Tamy |
dc.contributor.referee2Lattes.fl_str_mv |
http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4707954P2 |
contributor_str_mv |
Guimarães, Marta Fonseca Martins Lopes, Paulo Sávio Guimarães, Simone Eliza Facioni Guimarães, José Domingos Iguma, Lilian Tamy |
dc.subject.por.fl_str_mv |
PCR quantitativo em tempo real Expressão gênica Prolificidade |
topic |
PCR quantitativo em tempo real Expressão gênica Prolificidade Quantitative real time PCR Gene expression Prolificity CNPQ::CIENCIAS AGRARIAS::ZOOTECNIA::GENETICA E MELHORAMENTO DOS ANIMAIS DOMESTICOS |
dc.subject.eng.fl_str_mv |
Quantitative real time PCR Gene expression Prolificity |
dc.subject.cnpq.fl_str_mv |
CNPQ::CIENCIAS AGRARIAS::ZOOTECNIA::GENETICA E MELHORAMENTO DOS ANIMAIS DOMESTICOS |
description |
Among the productive traits, the number of the piglets is essential for success in swine production. The ovulation rate is one of the key factors that determine the litter size in pigs and the study of gene expression in the ovary follicles, more precisely in the follicular cells, might cause a big advance in the comprehension of this characteristic. Thus, the objective in this work was to identify the differential expression of the STAR (Steroidogenic Acute Regulator-STAR), GATA (GATA binding protein 4), PGF2α (Prostaglandin F2α), P4R (Progesterone Receptor), FSHR (Follicle Stimulating Hormone Receptor) and CYP19 (Cytochrome Aromatase P450) genes in follicular cells, colleted during the follicular phase in the estrous cycle of three sows with high and three sows with low litter size which comes from a tricross of the Landrace, Large White and Pietrain breeds, throughout the semi quantitative real time PCR (qPCR). In the hyperprolific sows, analyzed in this study, the average of the number of the piglets were 8,51 and for the hypoprolific sows the average was 12,03. For examination if any one related genes above were affecting the ovulation rate in the prolificity rate in the animals studied, the total RNA of the follicular liquid was extracted and than was made two pools with the total RNA of the hypo and hyperprolific sows groups. The total RNA of each pool was used for the first strand cDNA synthesis. The PCR reactions were accomplished using as an endogenous control the β-Actina gene. The primers were generated by sequences in the Pig ESTs data base. The major expression difference was observed for P4R gene, who express 7,73 turns mostly in the animals with low prolificity. The PGF2α gene displayed differential expression of 2,84 times as a large in the hypoprolific animals. The STAR gene expression, that codes for the protein regulating hormone steroids synthesis, was 2.32 turns mostly in the animals with low prolificity sows. The GATA gene expression was 2.31 times as a large in the hypoprolific sows. This gene codes for the GATA binding protein 4 belonging a transcription group factors that are responsible for too many gene expression and diverse cell differentiation types. The CYP19 gene expression taken the enzyme production that is the key factor in the estrogens biosynthesis, the Cytochrome Aromatase P450 (P450aro), for this gene was observed the relative expression of the 2.30 turns mostly in the sows with low prolificity. The FSHR gene expression hasn t achieved the threshold difference established in this study (1.5 times).The STAR, GATA, PGF2α, FSHR, P4R and CYP19 gene expression in the follicular cells has enabled identify difference of expression between sows with low and high prolificity, where the largest difference of expression in relation to the studied genes was verify in the hypoprolific sows, except for the FSHR gene that wasn t considerate differentially express in this work. With views the best agreement about the reproductive phenotypes in swine, it s necessary yet, studies to examine the expression in these and other genes related to female reproductive cycle, in as much as gene expression is dependent, in the midst of another factors, the stage of development of the tissue and the animal age. |
publishDate |
2007 |
dc.date.available.fl_str_mv |
2007-10-11 2015-03-26T13:42:36Z |
dc.date.issued.fl_str_mv |
2007-07-13 |
dc.date.accessioned.fl_str_mv |
2015-03-26T13:42:36Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/masterThesis |
format |
masterThesis |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
MIRANDA, Cristiana Libardi. Gene expression diferentialy in follicular cells in swine. 2007. 56 f. Dissertação (Mestrado em Genética animal; Genética molecular e de microrganismos; Genética quantitativa; Genética vegetal; Me) - Universidade Federal de Viçosa, Viçosa, 2007. |
dc.identifier.uri.fl_str_mv |
http://locus.ufv.br/handle/123456789/4821 |
identifier_str_mv |
MIRANDA, Cristiana Libardi. Gene expression diferentialy in follicular cells in swine. 2007. 56 f. Dissertação (Mestrado em Genética animal; Genética molecular e de microrganismos; Genética quantitativa; Genética vegetal; Me) - Universidade Federal de Viçosa, Viçosa, 2007. |
url |
http://locus.ufv.br/handle/123456789/4821 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
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openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Universidade Federal de Viçosa |
dc.publisher.program.fl_str_mv |
Mestrado em Genética e Melhoramento |
dc.publisher.initials.fl_str_mv |
UFV |
dc.publisher.country.fl_str_mv |
BR |
dc.publisher.department.fl_str_mv |
Genética animal; Genética molecular e de microrganismos; Genética quantitativa; Genética vegetal; Me |
publisher.none.fl_str_mv |
Universidade Federal de Viçosa |
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