Expressão diferencial de genes em células foliculares de suínos

• Autor(a) principal: Miranda, Cristiana Libardi
• Data de Publicação: 2007
• Tipo de documento: Dissertação
• Idioma: por
• Título da fonte: LOCUS Repositório Institucional da UFV
• Texto Completo: http://locus.ufv.br/handle/123456789/4821
• topic: PCR quantitativo em tempo real; Expressão gênica; Prolificidade; Quantitative real time PCR; Gene expression; Prolificity; CNPQ::CIENCIAS AGRARIAS::ZOOTECNIA::GENETICA E MELHORAMENTO DOS ANIMAIS DOMESTICOS

Among the productive traits, the number of the piglets is essential for success in swine production. The ovulation rate is one of the key factors that determine the litter size in pigs and the study of gene expression in the ovary follicles, more precisely in the follicular cells, might cause a big advance in the comprehension of this characteristic. Thus, the objective in this work was to identify the differential expression of the STAR (Steroidogenic Acute Regulator-STAR), GATA (GATA binding protein 4), PGF2α (Prostaglandin F2α), P4R (Progesterone Receptor), FSHR (Follicle Stimulating Hormone Receptor) and CYP19 (Cytochrome Aromatase P450) genes in follicular cells, colleted during the follicular phase in the estrous cycle of three sows with high and three sows with low litter size which comes from a tricross of the Landrace, Large White and Pietrain breeds, throughout the semi quantitative real time PCR (qPCR). In the hyperprolific sows, analyzed in this study, the average of the number of the piglets were 8,51 and for the hypoprolific sows the average was 12,03. For examination if any one related genes above were affecting the ovulation rate in the prolificity rate in the animals studied, the total RNA of the follicular liquid was extracted and than was made two pools with the total RNA of the hypo and hyperprolific sows groups. The total RNA of each pool was used for the first strand cDNA synthesis. The PCR reactions were accomplished using as an endogenous control the β-Actina gene. The primers were generated by sequences in the Pig ESTs data base. The major expression difference was observed for P4R gene, who express 7,73 turns mostly in the animals with low prolificity. The PGF2α gene displayed differential expression of 2,84 times as a large in the hypoprolific animals. The STAR gene expression, that codes for the protein regulating hormone steroids synthesis, was 2.32 turns mostly in the animals with low prolificity sows. The GATA gene expression was 2.31 times as a large in the hypoprolific sows. This gene codes for the GATA binding protein 4 belonging a transcription group factors that are responsible for too many gene expression and diverse cell differentiation types. The CYP19 gene expression taken the enzyme production that is the key factor in the estrogens biosynthesis, the Cytochrome Aromatase P450 (P450aro), for this gene was observed the relative expression of the 2.30 turns mostly in the sows with low prolificity. The FSHR gene expression hasn t achieved the threshold difference established in this study (1.5 times).The STAR, GATA, PGF2α, FSHR, P4R and CYP19 gene expression in the follicular cells has enabled identify difference of expression between sows with low and high prolificity, where the largest difference of expression in relation to the studied genes was verify in the hypoprolific sows, except for the FSHR gene that wasn t considerate differentially express in this work. With views the best agreement about the reproductive phenotypes in swine, it s necessary yet, studies to examine the expression in these and other genes related to female reproductive cycle, in as much as gene expression is dependent, in the midst of another factors, the stage of development of the tissue and the animal age.