A practical and reliable procedure for in vitro induction of tetraploid tomato
Main Author: | |
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Publication Date: | 2009 |
Other Authors: | , |
Format: | Article |
Language: | eng |
Source: | LOCUS Repositório Institucional da UFV |
Download full: | https://doi.org/10.1016/j.scienta.2009.05.032 http://www.locus.ufv.br/handle/123456789/22692 |
Summary: | A relatively rapid, practical and reliable procedure for in vitro production of tetraploid tomato using colchicine is described. Using this procedure 11.11% generation of tetraploids was obtained after exposure of seedling shoot meristem explants with 8 mM colchicine for 96 h. Confirmation of tetraploid production (2n = 48), was determined by flow cytometry and verified by cytogenetic analysis of root tip preparations. The results indicate that the procedure is adequate for induction and screening of tetraploid tomato plantlets. |
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Praça, Milene MirandaCarvalho, Carlos Roberto deClarindo, Wellington Ronildo2018-12-05T10:08:23Z2018-12-05T10:08:23Z2009-10-010304-4238https://doi.org/10.1016/j.scienta.2009.05.032http://www.locus.ufv.br/handle/123456789/22692A relatively rapid, practical and reliable procedure for in vitro production of tetraploid tomato using colchicine is described. Using this procedure 11.11% generation of tetraploids was obtained after exposure of seedling shoot meristem explants with 8 mM colchicine for 96 h. Confirmation of tetraploid production (2n = 48), was determined by flow cytometry and verified by cytogenetic analysis of root tip preparations. The results indicate that the procedure is adequate for induction and screening of tetraploid tomato plantlets.engScientia HorticulturaeVolume 122, Issue 3, Pages 501- 505, October 20092009 Elsevier B.V. All rights reserved.info:eu-repo/semantics/openAccessColchicineChromosomeFlow cytometrySolanum lycopersicumA practical and reliable procedure for in vitro induction of tetraploid tomatoinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfreponame:LOCUS Repositório Institucional da UFVinstname:Universidade Federal de Viçosa (UFV)instacron:UFVORIGINALartigo.pdfartigo.pdfTexto completoapplication/pdf180054https://locus.ufv.br//bitstream/123456789/22692/1/artigo.pdfaa1151902524423eb1cc2f8bde2e7427MD51LICENSElicense.txtlicense.txttext/plain; charset=utf-81748https://locus.ufv.br//bitstream/123456789/22692/2/license.txt8a4605be74aa9ea9d79846c1fba20a33MD52123456789/226922018-12-05 07:24:25.618oai:locus.ufv.br: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Repositório InstitucionalPUBhttps://www.locus.ufv.br/oai/requestfabiojreis@ufv.bropendoar:21452018-12-05T10:24:25LOCUS Repositório Institucional da UFV - Universidade Federal de Viçosa (UFV)false |
dc.title.en.fl_str_mv |
A practical and reliable procedure for in vitro induction of tetraploid tomato |
title |
A practical and reliable procedure for in vitro induction of tetraploid tomato |
spellingShingle |
A practical and reliable procedure for in vitro induction of tetraploid tomato Praça, Milene Miranda Colchicine Chromosome Flow cytometry Solanum lycopersicum |
title_short |
A practical and reliable procedure for in vitro induction of tetraploid tomato |
title_full |
A practical and reliable procedure for in vitro induction of tetraploid tomato |
title_fullStr |
A practical and reliable procedure for in vitro induction of tetraploid tomato |
title_full_unstemmed |
A practical and reliable procedure for in vitro induction of tetraploid tomato |
title_sort |
A practical and reliable procedure for in vitro induction of tetraploid tomato |
author |
Praça, Milene Miranda |
author_facet |
Praça, Milene Miranda Carvalho, Carlos Roberto de Clarindo, Wellington Ronildo |
author_role |
author |
author2 |
Carvalho, Carlos Roberto de Clarindo, Wellington Ronildo |
author2_role |
author author |
dc.contributor.author.fl_str_mv |
Praça, Milene Miranda Carvalho, Carlos Roberto de Clarindo, Wellington Ronildo |
dc.subject.pt-BR.fl_str_mv |
Colchicine Chromosome Flow cytometry Solanum lycopersicum |
topic |
Colchicine Chromosome Flow cytometry Solanum lycopersicum |
description |
A relatively rapid, practical and reliable procedure for in vitro production of tetraploid tomato using colchicine is described. Using this procedure 11.11% generation of tetraploids was obtained after exposure of seedling shoot meristem explants with 8 mM colchicine for 96 h. Confirmation of tetraploid production (2n = 48), was determined by flow cytometry and verified by cytogenetic analysis of root tip preparations. The results indicate that the procedure is adequate for induction and screening of tetraploid tomato plantlets. |
publishDate |
2009 |
dc.date.issued.fl_str_mv |
2009-10-01 |
dc.date.accessioned.fl_str_mv |
2018-12-05T10:08:23Z |
dc.date.available.fl_str_mv |
2018-12-05T10:08:23Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://doi.org/10.1016/j.scienta.2009.05.032 http://www.locus.ufv.br/handle/123456789/22692 |
dc.identifier.issn.none.fl_str_mv |
0304-4238 |
identifier_str_mv |
0304-4238 |
url |
https://doi.org/10.1016/j.scienta.2009.05.032 http://www.locus.ufv.br/handle/123456789/22692 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.ispartofseries.pt-BR.fl_str_mv |
Volume 122, Issue 3, Pages 501- 505, October 2009 |
dc.rights.driver.fl_str_mv |
2009 Elsevier B.V. All rights reserved. info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
2009 Elsevier B.V. All rights reserved. |
eu_rights_str_mv |
openAccess |
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application/pdf |
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Scientia Horticulturae |
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Scientia Horticulturae |
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LOCUS Repositório Institucional da UFV |
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