The population structure of Hemileia vastatrix in Brazil inferred from AFLP

• Autor(a) principal: Maia, T. A.
• Data de Publicação: 2013
• Outros Autores: Caixeta, E. T., Mizubuti, E. S. G., Zambolim, L., Maciel-Zambolim, E.
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: LOCUS Repositório Institucional da UFV
• Texto Completo: https://doi.org/10.1007/s13313-013-0213-3; http://www.locus.ufv.br/handle/123456789/23100
• topic: Coffee leaf rust; Durable resistance; Population genetics; Coffea arabica; Coffea canephora

Efforts to obtain durable resistant cultivars to coffee leaf rust have a long history of disappointment because of the lack of information on the Hemileia vastatrix evolutionary potential. In this study, the AFLP marker was used to analyze the genetic structure of a field population of H. vastatrix from different coffee genotypes cultivated in a wide geographic region of Brazil’s main production areas. A total of 91 isolates were sampled from genotypes of Coffea arabica, C. canephora and Híbrido de Timor/Icatu derivatives. Each isolate exhibited unique multilocus AFLP genotypes with no identical isolates obtained from the same geographical and host origins. This finding was corroborated by a small correlation between genetic similarity and geographic distance (R xy  = 0.31, P = 0.23). The analyses showed a low genetic differentiation (G ST  = 0.026) between the populations defined by a host and AMOVA analysis, most of the genetic variance (99.56 %) was attributed to differences within populations. In addition, based on the index of association (IA), the random mating possibility was not rejected (IA = 0.225, P = 0.123) for the population of H. vastatrix derived from C. canephora. This result suggested that H. vastatrix in Brazil behaves as a large undifferentiated population with a high level of genotypic diversity that is unstructured with regard to its geographic and host origins.