Fertilidade do sêmen caprino resfriado ou congelado em diferentes concentrações de gema-do-ovo no diluente

• Autor(a) principal: Bispo, Charles André Souza
• Data de Publicação: 2009
• Tipo de documento: Tese
• Idioma: por
• Título da fonte: LOCUS Repositório Institucional da UFV
• Texto Completo: http://locus.ufv.br/handle/123456789/1737
• topic: Sêmen; Caprino; Fertilidade; Semen; Goat; Fertility; CNPQ::CIENCIAS AGRARIAS::ZOOTECNIA::PRODUCAO ANIMAL

The experiments were conducted on Laboratory of Goat Reproduction in Goat Production Sector of Animal Science Department, Federal University of Viçosa, Zona da Mata, in Viçosa-MG. Three experiments were conducted; the first is related to cooling of semen and two others for freezing of goat semen. The first experiment was conducted during the physiological breeding season of goats in Brazil (March-June). The objective of this study was to evaluate, through in vitro and in vivo, what the concentrations of egg yolk (treatment 1 - T1 = 20% or Treatment 2 - T2 = 2.5%) preserve better the goat semen stored at 5 °C for 24 hours in citrate-egg yolk extender. The average values for freshsemen of volume (mL), motility (%), vigor (1-5), sperm concentration (x109 total), hypoosmotic swelling test (%) and supravital staining (%) were 0.7 ± 0.3, 87.9 ± 3.3, 3.9 ± 0.3, 1.3 ± 0.6, 72.7 ± 7.8 and 78.2 ± 9.3, respectively. The average values for cooled semen by the treatments were, respectively, for T1and T2: motility, 68.5 ± 15.4 and 78.0 ± 5.5, vigor, 2.5 ± 0.6 and 3 2 ± 0.3, supravital, 59.4 ± 17.2 and 71.1 ± 10.5 and hypoosmotic, 42.2 ± 15.7 and 56.2 ± 11.5 (p <0.05). Fertility rates obtained were 31.2% (T1) and 66.6% (T2) (p <0.05) and prolificacy was 1.3 ± 0.5 (T1) and 1.4 ± 0, 5 (T2) (p <0.05). According to the results, we can recommend the use of this extender with a low concentration of egg yolk (2.5%) in total volume. Experiments 2 and 3 were performed during the breeding season induced by supplementary light (November-December 2007) and physiological season (March to June 2008). The objective of this study was to evaluate, through tests in vitro and fertility in vivo, what is the concentrations of egg yolkof egg (25 and 2.5%) present in the extender (Martin et al.1979) can better preserve frozen goat semen during the different seasons. The average values obtained in Experiment 2 for fresh semen, as the volume, motility, vigor, sperm concentration, hypoosmotic swelling test and supravital staining, were 1.3 ± 0.4, 87.5 ± 2, 6 and 3.8 ± 0.3,respectively, and in Experiment 3, 1, 2 ± 0.1, 90.0 ± 3.7 and 3.8 ± 0.4, respectively. The average values for frozen semen of Experiment 2 were, respectively, for T1 and T2: motility, 58.7 ± 5.1% and 61.2 ± 5.1%; vigor, 2.7 ± 0.3 and 3.1 ± 0.2; supravital, 58.7 ± 7.5 and 61.7 ± 6.15. In Experiment 3 were respectively for T1 and T2: motility, 54.3 ± 6.2% and 60.6 ± 4.1%; vigor, 2.6 ± 0.2 and 3.1 ± 0.2; supravital , 56.3 ± 8.1 and 57.7 ± 6.8. Fertility rates obtained were 46.67% (T1) and 70.00% (T2) in Experiment 2. In Experiment 3 the fertility rates were 50.00% (T1) and 76.00% (T2). According to the results obtained, we can recommend the use of glucose-EDTA extender with low concentration of egg yolk (2.5%) in total volume.