Análises fisiológicas e bioquímicas da resistência do algodão à mancha de ramulária mediada pelo silício

Detalhes bibliográficos
Autor(a) principal: Curvêlo, Carmen Rosa da Silva
Data de Publicação: 2012
Tipo de documento: Tese
Idioma: por
Título da fonte: LOCUS Repositório Institucional da UFV
Texto Completo: http://locus.ufv.br/handle/123456789/1213
Resumo: The occurrence of diseases is a major problem that affects most of the cropland in Brazil, especially those located in the Midwest of region. Although silicon (Si) is not considered an essential element in plants, this nutrient has been shown to reduce the severity of many diseases in many plant species when accumulated in the shoot there by increasing plant resistance against pathogen attack. Respite this beneficial effect is widely reported in the literature, little is known about the Physiological and Biochemical mechanisms regulates by this element in the cotton-Ramularia areola pathosystem. Thus, the objective of this study was to evaluate the effect of Si in reducing Areolate mildew symptoms, as well as investigate the physiological and biochemical aspects of resistance mediated by this element. Cotton plants of the cultivars NuOpal (susceptible) and BRS Buriti (moderately resistant) were grown in pots with nutrient solution containing silicon (+Si) or not (-Si). The Si application reduced the area under the Areolate mildew progress curve (AUPCAM) in cotton plants. The increase in the concentration of phenolic compounds and derivatives of lignin-thioglycolic acid in plants of the two cultivars supplied with Si, and the increased activity of defense enzymes peroxidase (POX), polyphenol-oxidase (PPO), chitinase (CHI), ß-1, 3-glicanase (GLI) and phenylalanine ammonia-lyase (FAL) in plants of the cultivar NuOpal, potentiated the chemical defensive mechanisms against pathogen infection, as reported in the literature, but the PPO and FAL enzyme activity in BRS Buriti plants were not enhanced by Si. The provision of Si to the cotton plants of the two cultivars resulted in higher concentrations of H2O2 by reducing the activity of CAT, while increases in the activity of the enzymes APX, SOD and GR may have contributed to reduce the concentrations of MDA and EE for both cultivars. There were significant increases in LOX activity in plants supplied with Si of both cultivars. The supply of Si cotton plants of two cultivars increased the net rate of fixation of CO2 (A) in parallel with increases in stomatal conductance (gs). The concentrations of chlorophyll a (cla), chlorophyll b (clb) were higher in plants supplied with Si at later stages of infection to grow NuOpal but carotenoids were those who suffered less alteration.
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spelling Curvêlo, Carmen Rosa da Silvahttp://lattes.cnpq.br/8224358504381618Rodrigues, Fabrício de ávilahttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4709080E6Damatta, Fábio Murilohttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4784185Y9Berger, Paulo Geraldohttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4721101J6Cunha, Luis Claudio Vieira dahttp://lattes.cnpq.br/9367947173322278Paula Júnior, Trazilbo José dehttp://lattes.cnpq.br/78992760970188762015-03-26T12:43:55Z2014-01-212015-03-26T12:43:55Z2012-02-07CURVÊLO, Carmen Rosa da Silva. Physiological and biochemical analysis of the resistance of cotton to Areolate mildew mediated by silicon. 2012. 115 f. Tese (Doutorado em Plantas daninhas, Alelopatia, Herbicidas e Resíduos; Fisiologia de culturas; Manejo pós-colheita de) - Universidade Federal de Viçosa, Viçosa, 2012.http://locus.ufv.br/handle/123456789/1213The occurrence of diseases is a major problem that affects most of the cropland in Brazil, especially those located in the Midwest of region. Although silicon (Si) is not considered an essential element in plants, this nutrient has been shown to reduce the severity of many diseases in many plant species when accumulated in the shoot there by increasing plant resistance against pathogen attack. Respite this beneficial effect is widely reported in the literature, little is known about the Physiological and Biochemical mechanisms regulates by this element in the cotton-Ramularia areola pathosystem. Thus, the objective of this study was to evaluate the effect of Si in reducing Areolate mildew symptoms, as well as investigate the physiological and biochemical aspects of resistance mediated by this element. Cotton plants of the cultivars NuOpal (susceptible) and BRS Buriti (moderately resistant) were grown in pots with nutrient solution containing silicon (+Si) or not (-Si). The Si application reduced the area under the Areolate mildew progress curve (AUPCAM) in cotton plants. The increase in the concentration of phenolic compounds and derivatives of lignin-thioglycolic acid in plants of the two cultivars supplied with Si, and the increased activity of defense enzymes peroxidase (POX), polyphenol-oxidase (PPO), chitinase (CHI), ß-1, 3-glicanase (GLI) and phenylalanine ammonia-lyase (FAL) in plants of the cultivar NuOpal, potentiated the chemical defensive mechanisms against pathogen infection, as reported in the literature, but the PPO and FAL enzyme activity in BRS Buriti plants were not enhanced by Si. The provision of Si to the cotton plants of the two cultivars resulted in higher concentrations of H2O2 by reducing the activity of CAT, while increases in the activity of the enzymes APX, SOD and GR may have contributed to reduce the concentrations of MDA and EE for both cultivars. There were significant increases in LOX activity in plants supplied with Si of both cultivars. The supply of Si cotton plants of two cultivars increased the net rate of fixation of CO2 (A) in parallel with increases in stomatal conductance (gs). The concentrations of chlorophyll a (cla), chlorophyll b (clb) were higher in plants supplied with Si at later stages of infection to grow NuOpal but carotenoids were those who suffered less alteration.A ocorrência de doenças é um problema que afeta boa parte das áreas cultivadas no Brasil, sobretudo aquelas situadas na região centro-oeste do país. Apesar do silício (Si) não ser considerado um elemento essencial as plantas, esse nutriente reduz a severidade de inúmeras doenças em diversas espécies vegetais, aumentando a resistência das plantas ao ataque por patógenos. Contudo, apesar deste efeito benéfico ser amplamente relatado na literatura, pouco se sabe sobre os mecanismos envolvidos por esse elemento no patossistema algodão-Ramularia areola. Desta forma, o objetivo desse trabalho foi avaliar o efeito do Si em reduzir os sintomas da mancha de ramulária, bem como investigar os aspectos fisiológicos e bioquímicos da resistência mediada por esse elemento. Plantas de algodão das cultivares NuOpal (susceptível) e BRS Buriti (moderadamente resistente) foram cultivadas em vasos com solução nutritiva contendo (+Si) ou não (-Si) Si. A aplicação de Si reduziu a área abaixo da curva de progresso da mancha de ramulária (AACPMR) e aumentou a concentração de compostos fenólicos e de derivados da lignina-ácido tioglicólico nas plantas de algodão das duas cultivares, e aumentou a atividade das enzimas de defesa peroxidase (POX), polifenoloxidase (PFO), quitinase (QUI), ß-1,3-glicanases (GLI) e da fenilalanina amônia-liases (FAL) nas plantas da cultivar NuOpal, porém a atividade das enzimas PFO e FAL nas plantas da cultivar BRS Buriti não foram potencializadas pelo Si. O fornecimento de Si às plantas de algodão das duas cultivares ocasionaram em menores concentrações de peróxido de hidrogênio (H2O2) e redução na atividade da catalase (CAT), enquanto que aumentos na atividade das enzimas APX, SOD e GR podem ter contribuído para reduzir as concentrações de MDA e EE para as duas cultivares. Houve aumentos na atividade da lipoxigenase (LOX) em plantas supridas com Si das duas cultivares e reduções nos parâmetros de trocas gasosas. O fornecimento de Si as plantas de algodão das duas cultivares aumentou a taxa líquida de fixação de CO2 (A) em paralelo a aumentos em condutância estomática (gs). As concentrações de clorofila a (cla), clorofila b (clb) foram maiores nas plantas supridas com Si em estádios mais avançados da infecção para cultivar NuOpal, porém os carotenóides foram os que sofreram menor alteração.Conselho Nacional de Desenvolvimento Científico e Tecnológicoapplication/pdfporUniversidade Federal de ViçosaDoutorado em FitotecniaUFVBRPlantas daninhas, Alelopatia, Herbicidas e Resíduos; Fisiologia de culturas; Manejo pós-colheita deAlgodãoEnzimologiaFotossínteseMancha de ramuláriaCottonEnzymologyPhotosynthesisAreolate mildewCNPQ::CIENCIAS AGRARIAS::AGRONOMIA::FITOTECNIAAnálises fisiológicas e bioquímicas da resistência do algodão à mancha de ramulária mediada pelo silícioPhysiological and biochemical analysis of the resistance of cotton to Areolate mildew mediated by siliconinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/doctoralThesisinfo:eu-repo/semantics/openAccessreponame:LOCUS Repositório Institucional da UFVinstname:Universidade Federal de Viçosa (UFV)instacron:UFVORIGINALtexto completo.pdfapplication/pdf1074487https://locus.ufv.br//bitstream/123456789/1213/1/texto%20completo.pdf84e818b82b4a5eec1ebf16f361b5b91eMD51TEXTtexto completo.pdf.txttexto completo.pdf.txtExtracted texttext/plain214717https://locus.ufv.br//bitstream/123456789/1213/2/texto%20completo.pdf.txt349c6f4549b62a656ed40a2f1f4ff76dMD52THUMBNAILtexto completo.pdf.jpgtexto completo.pdf.jpgIM Thumbnailimage/jpeg3625https://locus.ufv.br//bitstream/123456789/1213/3/texto%20completo.pdf.jpgc557fae78757fb1b68ed01af17d6d326MD53123456789/12132016-04-07 23:02:06.682oai:locus.ufv.br:123456789/1213Repositório InstitucionalPUBhttps://www.locus.ufv.br/oai/requestfabiojreis@ufv.bropendoar:21452016-04-08T02:02:06LOCUS Repositório Institucional da UFV - Universidade Federal de Viçosa (UFV)false
dc.title.por.fl_str_mv Análises fisiológicas e bioquímicas da resistência do algodão à mancha de ramulária mediada pelo silício
dc.title.alternative.eng.fl_str_mv Physiological and biochemical analysis of the resistance of cotton to Areolate mildew mediated by silicon
title Análises fisiológicas e bioquímicas da resistência do algodão à mancha de ramulária mediada pelo silício
spellingShingle Análises fisiológicas e bioquímicas da resistência do algodão à mancha de ramulária mediada pelo silício
Curvêlo, Carmen Rosa da Silva
Algodão
Enzimologia
Fotossíntese
Mancha de ramulária
Cotton
Enzymology
Photosynthesis
Areolate mildew
CNPQ::CIENCIAS AGRARIAS::AGRONOMIA::FITOTECNIA
title_short Análises fisiológicas e bioquímicas da resistência do algodão à mancha de ramulária mediada pelo silício
title_full Análises fisiológicas e bioquímicas da resistência do algodão à mancha de ramulária mediada pelo silício
title_fullStr Análises fisiológicas e bioquímicas da resistência do algodão à mancha de ramulária mediada pelo silício
title_full_unstemmed Análises fisiológicas e bioquímicas da resistência do algodão à mancha de ramulária mediada pelo silício
title_sort Análises fisiológicas e bioquímicas da resistência do algodão à mancha de ramulária mediada pelo silício
author Curvêlo, Carmen Rosa da Silva
author_facet Curvêlo, Carmen Rosa da Silva
author_role author
dc.contributor.authorLattes.por.fl_str_mv http://lattes.cnpq.br/8224358504381618
dc.contributor.author.fl_str_mv Curvêlo, Carmen Rosa da Silva
dc.contributor.advisor-co1.fl_str_mv Rodrigues, Fabrício de ávila
dc.contributor.advisor-co1Lattes.fl_str_mv http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4709080E6
dc.contributor.advisor-co2.fl_str_mv Damatta, Fábio Murilo
dc.contributor.advisor-co2Lattes.fl_str_mv http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4784185Y9
dc.contributor.advisor1.fl_str_mv Berger, Paulo Geraldo
dc.contributor.advisor1Lattes.fl_str_mv http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4721101J6
dc.contributor.referee1.fl_str_mv Cunha, Luis Claudio Vieira da
dc.contributor.referee1Lattes.fl_str_mv http://lattes.cnpq.br/9367947173322278
dc.contributor.referee2.fl_str_mv Paula Júnior, Trazilbo José de
dc.contributor.referee2Lattes.fl_str_mv http://lattes.cnpq.br/7899276097018876
contributor_str_mv Rodrigues, Fabrício de ávila
Damatta, Fábio Murilo
Berger, Paulo Geraldo
Cunha, Luis Claudio Vieira da
Paula Júnior, Trazilbo José de
dc.subject.por.fl_str_mv Algodão
Enzimologia
Fotossíntese
Mancha de ramulária
topic Algodão
Enzimologia
Fotossíntese
Mancha de ramulária
Cotton
Enzymology
Photosynthesis
Areolate mildew
CNPQ::CIENCIAS AGRARIAS::AGRONOMIA::FITOTECNIA
dc.subject.eng.fl_str_mv Cotton
Enzymology
Photosynthesis
Areolate mildew
dc.subject.cnpq.fl_str_mv CNPQ::CIENCIAS AGRARIAS::AGRONOMIA::FITOTECNIA
description The occurrence of diseases is a major problem that affects most of the cropland in Brazil, especially those located in the Midwest of region. Although silicon (Si) is not considered an essential element in plants, this nutrient has been shown to reduce the severity of many diseases in many plant species when accumulated in the shoot there by increasing plant resistance against pathogen attack. Respite this beneficial effect is widely reported in the literature, little is known about the Physiological and Biochemical mechanisms regulates by this element in the cotton-Ramularia areola pathosystem. Thus, the objective of this study was to evaluate the effect of Si in reducing Areolate mildew symptoms, as well as investigate the physiological and biochemical aspects of resistance mediated by this element. Cotton plants of the cultivars NuOpal (susceptible) and BRS Buriti (moderately resistant) were grown in pots with nutrient solution containing silicon (+Si) or not (-Si). The Si application reduced the area under the Areolate mildew progress curve (AUPCAM) in cotton plants. The increase in the concentration of phenolic compounds and derivatives of lignin-thioglycolic acid in plants of the two cultivars supplied with Si, and the increased activity of defense enzymes peroxidase (POX), polyphenol-oxidase (PPO), chitinase (CHI), ß-1, 3-glicanase (GLI) and phenylalanine ammonia-lyase (FAL) in plants of the cultivar NuOpal, potentiated the chemical defensive mechanisms against pathogen infection, as reported in the literature, but the PPO and FAL enzyme activity in BRS Buriti plants were not enhanced by Si. The provision of Si to the cotton plants of the two cultivars resulted in higher concentrations of H2O2 by reducing the activity of CAT, while increases in the activity of the enzymes APX, SOD and GR may have contributed to reduce the concentrations of MDA and EE for both cultivars. There were significant increases in LOX activity in plants supplied with Si of both cultivars. The supply of Si cotton plants of two cultivars increased the net rate of fixation of CO2 (A) in parallel with increases in stomatal conductance (gs). The concentrations of chlorophyll a (cla), chlorophyll b (clb) were higher in plants supplied with Si at later stages of infection to grow NuOpal but carotenoids were those who suffered less alteration.
publishDate 2012
dc.date.issued.fl_str_mv 2012-02-07
dc.date.available.fl_str_mv 2014-01-21
2015-03-26T12:43:55Z
dc.date.accessioned.fl_str_mv 2015-03-26T12:43:55Z
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dc.identifier.citation.fl_str_mv CURVÊLO, Carmen Rosa da Silva. Physiological and biochemical analysis of the resistance of cotton to Areolate mildew mediated by silicon. 2012. 115 f. Tese (Doutorado em Plantas daninhas, Alelopatia, Herbicidas e Resíduos; Fisiologia de culturas; Manejo pós-colheita de) - Universidade Federal de Viçosa, Viçosa, 2012.
dc.identifier.uri.fl_str_mv http://locus.ufv.br/handle/123456789/1213
identifier_str_mv CURVÊLO, Carmen Rosa da Silva. Physiological and biochemical analysis of the resistance of cotton to Areolate mildew mediated by silicon. 2012. 115 f. Tese (Doutorado em Plantas daninhas, Alelopatia, Herbicidas e Resíduos; Fisiologia de culturas; Manejo pós-colheita de) - Universidade Federal de Viçosa, Viçosa, 2012.
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dc.publisher.department.fl_str_mv Plantas daninhas, Alelopatia, Herbicidas e Resíduos; Fisiologia de culturas; Manejo pós-colheita de
publisher.none.fl_str_mv Universidade Federal de Viçosa
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