Evaluation of the genotoxic potential of ethanolic extracts of stem bark and leaves of Bathysa cuspidata (A.St.-Hil.) Hook

Detalhes bibliográficos
Autor(a) principal: Gontijo, Douglas Costa
Data de Publicação: 2012
Outros Autores: Nunes, Líria Granato, Souza, Carlos Joulbert Alves, Fietto, Luciano Gomes, Leite, João Paulo Viana
Tipo de documento: Artigo
Idioma: eng
Título da fonte: LOCUS Repositório Institucional da UFV
Texto Completo: http://serv-bib.fcfar.unesp.br/seer/index.php/Cien_Farm/article/viewArticle/1980
http://www.locus.ufv.br/handle/123456789/18006
Resumo: DOI: não consta, por isso foi disponibilizado o link de acesso.
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spelling Gontijo, Douglas CostaNunes, Líria GranatoSouza, Carlos Joulbert AlvesFietto, Luciano GomesLeite, João Paulo Viana2018-02-28T17:29:12Z2018-02-28T17:29:12Z2012-03-071808-4532http://serv-bib.fcfar.unesp.br/seer/index.php/Cien_Farm/article/viewArticle/1980http://www.locus.ufv.br/handle/123456789/18006DOI: não consta, por isso foi disponibilizado o link de acesso.Bathysa cuspidata(A.St.-Hil.) Hook. é uma árvore nativa do bioma Mata Atlântica largamente utilizada na medicina popular brasileira. Apesar do amplo uso, ainda não existe relato sobre a toxicologia dos compostos obtidos dessa espécie vegetal. O presente estudo avaliou efeitos mutagênicos e genotóxicos de extratos etanólicos de B. cuspidataobtidos das cascas do caule (SBC) e das folhas (LBC). A mutagenicidade dos extratos foi avaliada utilizando o teste de Ames nas linhagens TA98 e TA100 de Salmonella typhimurium, na ausência e presença de ativação metabólica. Os efeitos dos extratos diretamente sobre o DNA foram avaliados por clivagem plasmidial. Análises fitoquímicas foram realizadas para comparar a composição de metabólitos secundários dos extratos. Não foi encontrada atividade mutagênica para LBC quando avaliados utilizando as cepas TA98 e TA100, sem ou com ativação metabólica. No entanto, SBC apresentou atividade mutagênica. Clivagem plasmidial não indicou ação genotóxica tanto para SBC quanto para LBC. Diferenças na composição dos metabólitos secundários presentes nos cascas e folhas, detectadas pelas análises fitoquímicas, devem ser determinantes para a variação do efeito mutagênico entre SBC e LBC. Os resultados sugerem cautela no emprego de cascas de B. cuspidata.Bathysa cuspidata(A.St.-Hil.) Hook. is a native tree of the Brazilian Atlantic Forest biome, widely used in Brazilian herbal medicine. Despite its widespread use, there is no report as yet regarding the toxicology of this plant. In this study, the mutagenic and genotoxic effects of ethanolic extracts of B. cuspidatastem bark (SBC) and leaves (LBC) were assessed. The mutagenicity of the extracts was estimated by performing the Ames test on strains TA98 and TA100 of Salmonella typhimurium, in the absence and presence of metabolic activation. The direct action of the extracts on DNA was assessed through plasmid treatment. Phytochemical screening was conducted to compare the secondary metabolite composition of the extracts. No mutagenic activity was found in LBC when tested on strain TA98 or TA100, without or with metabolic activation. However, SBC did show mutagenic activity. Plasmid tests did not indicate genotoxic action for either SBC or LBC. Differences in the composition of secondary metabolites present in the bark and leaves, detected by phytochemical analysis, appear to be a deciding factor in differences in mutagenicity between SBC and LBC. The findings in this study suggest caution in the use of B. cuspidata bark.engRevista de Ciências Farmacêuticas Básica e Aplicadav.33, n.3, p.355-359, 2012Bathysa cuspidataGenotoxicityMutagenesisAmes testEvaluation of the genotoxic potential of ethanolic extracts of stem bark and leaves of Bathysa cuspidata (A.St.-Hil.) 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dc.title.en.fl_str_mv Evaluation of the genotoxic potential of ethanolic extracts of stem bark and leaves of Bathysa cuspidata (A.St.-Hil.) Hook
title Evaluation of the genotoxic potential of ethanolic extracts of stem bark and leaves of Bathysa cuspidata (A.St.-Hil.) Hook
spellingShingle Evaluation of the genotoxic potential of ethanolic extracts of stem bark and leaves of Bathysa cuspidata (A.St.-Hil.) Hook
Gontijo, Douglas Costa
Bathysa cuspidata
Genotoxicity
Mutagenesis
Ames test
title_short Evaluation of the genotoxic potential of ethanolic extracts of stem bark and leaves of Bathysa cuspidata (A.St.-Hil.) Hook
title_full Evaluation of the genotoxic potential of ethanolic extracts of stem bark and leaves of Bathysa cuspidata (A.St.-Hil.) Hook
title_fullStr Evaluation of the genotoxic potential of ethanolic extracts of stem bark and leaves of Bathysa cuspidata (A.St.-Hil.) Hook
title_full_unstemmed Evaluation of the genotoxic potential of ethanolic extracts of stem bark and leaves of Bathysa cuspidata (A.St.-Hil.) Hook
title_sort Evaluation of the genotoxic potential of ethanolic extracts of stem bark and leaves of Bathysa cuspidata (A.St.-Hil.) Hook
author Gontijo, Douglas Costa
author_facet Gontijo, Douglas Costa
Nunes, Líria Granato
Souza, Carlos Joulbert Alves
Fietto, Luciano Gomes
Leite, João Paulo Viana
author_role author
author2 Nunes, Líria Granato
Souza, Carlos Joulbert Alves
Fietto, Luciano Gomes
Leite, João Paulo Viana
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Gontijo, Douglas Costa
Nunes, Líria Granato
Souza, Carlos Joulbert Alves
Fietto, Luciano Gomes
Leite, João Paulo Viana
dc.subject.pt-BR.fl_str_mv Bathysa cuspidata
Genotoxicity
Mutagenesis
Ames test
topic Bathysa cuspidata
Genotoxicity
Mutagenesis
Ames test
description DOI: não consta, por isso foi disponibilizado o link de acesso.
publishDate 2012
dc.date.issued.fl_str_mv 2012-03-07
dc.date.accessioned.fl_str_mv 2018-02-28T17:29:12Z
dc.date.available.fl_str_mv 2018-02-28T17:29:12Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
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dc.identifier.uri.fl_str_mv http://serv-bib.fcfar.unesp.br/seer/index.php/Cien_Farm/article/viewArticle/1980
http://www.locus.ufv.br/handle/123456789/18006
dc.identifier.issn.none.fl_str_mv 1808-4532
identifier_str_mv 1808-4532
url http://serv-bib.fcfar.unesp.br/seer/index.php/Cien_Farm/article/viewArticle/1980
http://www.locus.ufv.br/handle/123456789/18006
dc.language.iso.fl_str_mv eng
language eng
dc.relation.ispartofseries.pt-BR.fl_str_mv v.33, n.3, p.355-359, 2012
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