Escherichia coli Shiga-Toxigênica (STEC) em abatedouro de bovinos no Estado de Minas Gerais
Autor(a) principal: | |
---|---|
Data de Publicação: | 2008 |
Tipo de documento: | Dissertação |
Idioma: | por |
Título da fonte: | LOCUS Repositório Institucional da UFV |
Texto Completo: | http://locus.ufv.br/handle/123456789/5376 |
Resumo: | Shiga-toxigenic Escherichia coli, STEC, has emerged as a pathogen that can cause food-borne infections and severe and potentially fatal diseases such as hemorrhagic colitis (HC) and hemolytic uremic syndrome (HUS). Most HC and HUS outbreaks have been attributed to strains of entero-hemorrhagic serotype O157:H7, but there is growing interest on the risk to human health associated with the non-O157 STEC serotypes carried by beef products contaminated by ruminant feces. This study aimed to determine the occurrence of STEC in a cattle slaughterhouse located in the State of Minas Gerais. Sampling was carried out in two lots; Lot A with 75 bovines and Lot B with 51 animals in three stages of the slaughtering line: removal of skin after bleeding, carcass after sawing, and feces during evisceration. Samples were collected from ten animals of each lot. The samples were enriched in modified EC broth (mEC) and modified EC added with novobiocin (mECn) followed by isolation of typical colonies on Sorbitol MacConkey Medium. Two hundred and forty-one bacterial isolates obtained from the two lots, were identified as E. coli. Of these, 104 were derived from the skin, 93 from fecal material and 44 from carcasses. Polymerase chain reaction detected the stx gene in 18 isolates of E. coli. These isolates were obtained from six animals: four were of fecal origin and come from lot A; two were from carcasses from lot B. Enrichment in mECn resulted in isolates originated from one animal, whereas in mEC, the isolates were originated from five bovines. Isolates stx positive were analyzed for the hlyA gene encoding enterohemolysin. Four hlyA positive isolates originated from two bovines, one from lot A and another from lot B. Two displayed the entero- hemolytic phenotype and were isolated from the carcass. Ten of the 18 potentially STEC isolates showed cytotoxic effect on Vero cells. Eight isolates from three different animals had the stx PCR amplicons sequenced. The translated sequences obtained from stx of three isolates from the carcass and of one isolate from feces corresponded to Stx2, showing 98% to 99% identity, with references at the GeneBank (NCBI). The sequences inferred from four amplicons obtained from isolates of faecal origin corresponded to Stx1, with 94% to 96% identity. The presence of STEC in the feces and carcasses of cattle at a slaughterhouse in the state of Minas Gerais emphasizes the importance of establishing a HACCP system and the need for implementation, evaluation, and validation of control measures to minimize the risk of cross-contamination during and after slaughter. |
id |
UFV_c317e5241173f6758b825c70b37ec904 |
---|---|
oai_identifier_str |
oai:locus.ufv.br:123456789/5376 |
network_acronym_str |
UFV |
network_name_str |
LOCUS Repositório Institucional da UFV |
repository_id_str |
2145 |
spelling |
Gomes, Andressa Pinheirohttp://lattes.cnpq.br/5822691880756911Teixeira, Magdala Alencarhttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4787866Y1Borges, Arnaldo Chaerhttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4783573Z8Moraes, Célia Alencar dehttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4781007D6Vanetti, Maria Cristina Dantashttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4783874H3Pinto, Cláudia Lúcia de Oliveirahttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4783521J62015-03-26T13:52:01Z2015-01-292015-03-26T13:52:01Z2008-10-03GOMES, Andressa Pinheiro. Escherichia coli Shiga-Toxigênica (STEC) in slaughterhouse of bovines in the State of Minas Gerais, Brazil. 2008. 64 f. Dissertação (Mestrado em Associações micorrízicas; Bactérias láticas e probióticos; Biologia molecular de fungos de interesse) - Universidade Federal de Viçosa, Viçosa, 2008.http://locus.ufv.br/handle/123456789/5376Shiga-toxigenic Escherichia coli, STEC, has emerged as a pathogen that can cause food-borne infections and severe and potentially fatal diseases such as hemorrhagic colitis (HC) and hemolytic uremic syndrome (HUS). Most HC and HUS outbreaks have been attributed to strains of entero-hemorrhagic serotype O157:H7, but there is growing interest on the risk to human health associated with the non-O157 STEC serotypes carried by beef products contaminated by ruminant feces. This study aimed to determine the occurrence of STEC in a cattle slaughterhouse located in the State of Minas Gerais. Sampling was carried out in two lots; Lot A with 75 bovines and Lot B with 51 animals in three stages of the slaughtering line: removal of skin after bleeding, carcass after sawing, and feces during evisceration. Samples were collected from ten animals of each lot. The samples were enriched in modified EC broth (mEC) and modified EC added with novobiocin (mECn) followed by isolation of typical colonies on Sorbitol MacConkey Medium. Two hundred and forty-one bacterial isolates obtained from the two lots, were identified as E. coli. Of these, 104 were derived from the skin, 93 from fecal material and 44 from carcasses. Polymerase chain reaction detected the stx gene in 18 isolates of E. coli. These isolates were obtained from six animals: four were of fecal origin and come from lot A; two were from carcasses from lot B. Enrichment in mECn resulted in isolates originated from one animal, whereas in mEC, the isolates were originated from five bovines. Isolates stx positive were analyzed for the hlyA gene encoding enterohemolysin. Four hlyA positive isolates originated from two bovines, one from lot A and another from lot B. Two displayed the entero- hemolytic phenotype and were isolated from the carcass. Ten of the 18 potentially STEC isolates showed cytotoxic effect on Vero cells. Eight isolates from three different animals had the stx PCR amplicons sequenced. The translated sequences obtained from stx of three isolates from the carcass and of one isolate from feces corresponded to Stx2, showing 98% to 99% identity, with references at the GeneBank (NCBI). The sequences inferred from four amplicons obtained from isolates of faecal origin corresponded to Stx1, with 94% to 96% identity. The presence of STEC in the feces and carcasses of cattle at a slaughterhouse in the state of Minas Gerais emphasizes the importance of establishing a HACCP system and the need for implementation, evaluation, and validation of control measures to minimize the risk of cross-contamination during and after slaughter.Escherichia coli Shiga-toxigênica, STEC, tem emergido como patógeno que pode causar infecções de origem alimentar e doenças severas e potencialmente fatais, como Colite Hemorrágica (CH) e Síndrome Urêmico Hemolítica (SUH). A maioria dos surtos de CH e SUH tem sido atribuída a estirpes do sorotipo entero-hemorrágico O157:H7, entretanto existe interesse crescente sobre o risco à saúde humana associado com os sorotipos STEC não-O157 veiculados em produtos de carne bovina, contaminados por fezes de ruminantes. Este trabalho teve como objetivos determinar a ocorrência de STEC em bovinos em matadouro frigorífico, localizado no Estado de Minas Gerais. A amostragem foi realizada em dois lotes, lote A com 75 bovinos e lote B com 51 bovinos, em três etapas da linha de abate: pele, após a sangria; carcaça, após a serragem; e fezes, durante a evisceração. Dez animais de cada lote foram amostrados. As amostras foram enriquecidas em caldo EC modificado, mEC e EC modificado acrescido de Novobiocina, mECn, seguindo-se o isolamento de colônias típicas em meio MacConkey sorbitol. Duzentos e quarenta e um isolados bacterianos obtidos nos dois lotes foram identificados como E. coli. Desses, 104 foram provenientes da pele, 93 do material fecal e 44 das carcaças. Por reação em cadeia de polimerase, foi detectado o gene stx em 18 isolados de E. coli. Esses isolados foram provenientes de seis bovinos, sendo quatro do lote A (origem fecal) e dois do lote B (carcaça). O enriquecimento em meio mECn resultou em isolados originados de um bovino, enquanto no mEC os isolados se originaram de cinco bovinos. Os isolados stx positivos foram posteriormente analisados para o gene hlyA, codificador de entero-hemolisina. Quatro isolados, positivos para o gene hlyA, foram provenientes de dois bovinos, um do lote A (origem fecal) e outro do lote B (carcaça). Desses, dois isolados da carcaça apresentaram fenótipo entero-hemolítico. Dez dos 18 isolados potencialmente STEC demonstraram ter efeito citotóxico em células Vero. Oito isolados, provenientes de três bovinos distintos, tiveram os amplicons stx seqüenciados. As seqüências traduzidas de três isolados da carcaça e de um das fezes corresponderam a Stx2, com 98% a 99% de identidade, com referências no GeneBank (NCBI). As seqüências inferidas de quatro amplicons, de origem fecal, corresponderam, com 94% a 96% de identidade, a Stx1. A presença de STEC nas fezes e carcaça de bovinos em matadouro frigorífico no Estado de Minas Gerais enfatiza a importância de implementação do sistema HACCP, e a necessidade de implementar, avaliar e validar medidas de controle que minimizem os riscos de contaminação cruzada durante o processo de abate.Conselho Nacional de Desenvolvimento Científico e Tecnológicoapplication/pdfporUniversidade Federal de ViçosaMestrado em Microbiologia AgrícolaUFVBRAssociações micorrízicas; Bactérias láticas e probióticos; Biologia molecular de fungos de interesseEscherichia coliToxinasBovinoMatadourosBactérias patogênicasReação em cadeia de polimeraseEscherichia coliToxinsCattleSlaughterhousesPathogenic bacteriaPolymerase chain reactionCNPQ::CIENCIAS AGRARIAS::CIENCIA E TECNOLOGIA DE ALIMENTOS::CIENCIA DE ALIMENTOSEscherichia coli Shiga-Toxigênica (STEC) em abatedouro de bovinos no Estado de Minas GeraisEscherichia coli Shiga-Toxigênica (STEC) in slaughterhouse of bovines in the State of Minas Gerais, Brazilinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisinfo:eu-repo/semantics/embargoedAccessreponame:LOCUS Repositório Institucional da UFVinstname:Universidade Federal de Viçosa (UFV)instacron:UFVORIGINALtexto completo.pdfapplication/pdf582896https://locus.ufv.br//bitstream/123456789/5376/1/texto%20completo.pdff0af95b88bc9afacbb9f1a952768a98eMD51TEXTtexto completo.pdf.txttexto completo.pdf.txtExtracted texttext/plain100559https://locus.ufv.br//bitstream/123456789/5376/2/texto%20completo.pdf.txt3fb79b62c145728a73a0dad9b7a5cbc4MD52THUMBNAILtexto completo.pdf.jpgtexto completo.pdf.jpgIM Thumbnailimage/jpeg3682https://locus.ufv.br//bitstream/123456789/5376/3/texto%20completo.pdf.jpgc12beacb20dbb15414fe21e10f2409bdMD53123456789/53762016-04-10 23:19:39.641oai:locus.ufv.br:123456789/5376Repositório InstitucionalPUBhttps://www.locus.ufv.br/oai/requestfabiojreis@ufv.bropendoar:21452016-04-11T02:19:39LOCUS Repositório Institucional da UFV - Universidade Federal de Viçosa (UFV)false |
dc.title.por.fl_str_mv |
Escherichia coli Shiga-Toxigênica (STEC) em abatedouro de bovinos no Estado de Minas Gerais |
dc.title.alternative.eng.fl_str_mv |
Escherichia coli Shiga-Toxigênica (STEC) in slaughterhouse of bovines in the State of Minas Gerais, Brazil |
title |
Escherichia coli Shiga-Toxigênica (STEC) em abatedouro de bovinos no Estado de Minas Gerais |
spellingShingle |
Escherichia coli Shiga-Toxigênica (STEC) em abatedouro de bovinos no Estado de Minas Gerais Gomes, Andressa Pinheiro Escherichia coli Toxinas Bovino Matadouros Bactérias patogênicas Reação em cadeia de polimerase Escherichia coli Toxins Cattle Slaughterhouses Pathogenic bacteria Polymerase chain reaction CNPQ::CIENCIAS AGRARIAS::CIENCIA E TECNOLOGIA DE ALIMENTOS::CIENCIA DE ALIMENTOS |
title_short |
Escherichia coli Shiga-Toxigênica (STEC) em abatedouro de bovinos no Estado de Minas Gerais |
title_full |
Escherichia coli Shiga-Toxigênica (STEC) em abatedouro de bovinos no Estado de Minas Gerais |
title_fullStr |
Escherichia coli Shiga-Toxigênica (STEC) em abatedouro de bovinos no Estado de Minas Gerais |
title_full_unstemmed |
Escherichia coli Shiga-Toxigênica (STEC) em abatedouro de bovinos no Estado de Minas Gerais |
title_sort |
Escherichia coli Shiga-Toxigênica (STEC) em abatedouro de bovinos no Estado de Minas Gerais |
author |
Gomes, Andressa Pinheiro |
author_facet |
Gomes, Andressa Pinheiro |
author_role |
author |
dc.contributor.authorLattes.por.fl_str_mv |
http://lattes.cnpq.br/5822691880756911 |
dc.contributor.author.fl_str_mv |
Gomes, Andressa Pinheiro |
dc.contributor.advisor-co1.fl_str_mv |
Teixeira, Magdala Alencar |
dc.contributor.advisor-co1Lattes.fl_str_mv |
http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4787866Y1 |
dc.contributor.advisor-co2.fl_str_mv |
Borges, Arnaldo Chaer |
dc.contributor.advisor-co2Lattes.fl_str_mv |
http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4783573Z8 |
dc.contributor.advisor1.fl_str_mv |
Moraes, Célia Alencar de |
dc.contributor.advisor1Lattes.fl_str_mv |
http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4781007D6 |
dc.contributor.referee1.fl_str_mv |
Vanetti, Maria Cristina Dantas |
dc.contributor.referee1Lattes.fl_str_mv |
http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4783874H3 |
dc.contributor.referee2.fl_str_mv |
Pinto, Cláudia Lúcia de Oliveira |
dc.contributor.referee2Lattes.fl_str_mv |
http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4783521J6 |
contributor_str_mv |
Teixeira, Magdala Alencar Borges, Arnaldo Chaer Moraes, Célia Alencar de Vanetti, Maria Cristina Dantas Pinto, Cláudia Lúcia de Oliveira |
dc.subject.por.fl_str_mv |
Escherichia coli Toxinas Bovino Matadouros Bactérias patogênicas Reação em cadeia de polimerase |
topic |
Escherichia coli Toxinas Bovino Matadouros Bactérias patogênicas Reação em cadeia de polimerase Escherichia coli Toxins Cattle Slaughterhouses Pathogenic bacteria Polymerase chain reaction CNPQ::CIENCIAS AGRARIAS::CIENCIA E TECNOLOGIA DE ALIMENTOS::CIENCIA DE ALIMENTOS |
dc.subject.eng.fl_str_mv |
Escherichia coli Toxins Cattle Slaughterhouses Pathogenic bacteria Polymerase chain reaction |
dc.subject.cnpq.fl_str_mv |
CNPQ::CIENCIAS AGRARIAS::CIENCIA E TECNOLOGIA DE ALIMENTOS::CIENCIA DE ALIMENTOS |
description |
Shiga-toxigenic Escherichia coli, STEC, has emerged as a pathogen that can cause food-borne infections and severe and potentially fatal diseases such as hemorrhagic colitis (HC) and hemolytic uremic syndrome (HUS). Most HC and HUS outbreaks have been attributed to strains of entero-hemorrhagic serotype O157:H7, but there is growing interest on the risk to human health associated with the non-O157 STEC serotypes carried by beef products contaminated by ruminant feces. This study aimed to determine the occurrence of STEC in a cattle slaughterhouse located in the State of Minas Gerais. Sampling was carried out in two lots; Lot A with 75 bovines and Lot B with 51 animals in three stages of the slaughtering line: removal of skin after bleeding, carcass after sawing, and feces during evisceration. Samples were collected from ten animals of each lot. The samples were enriched in modified EC broth (mEC) and modified EC added with novobiocin (mECn) followed by isolation of typical colonies on Sorbitol MacConkey Medium. Two hundred and forty-one bacterial isolates obtained from the two lots, were identified as E. coli. Of these, 104 were derived from the skin, 93 from fecal material and 44 from carcasses. Polymerase chain reaction detected the stx gene in 18 isolates of E. coli. These isolates were obtained from six animals: four were of fecal origin and come from lot A; two were from carcasses from lot B. Enrichment in mECn resulted in isolates originated from one animal, whereas in mEC, the isolates were originated from five bovines. Isolates stx positive were analyzed for the hlyA gene encoding enterohemolysin. Four hlyA positive isolates originated from two bovines, one from lot A and another from lot B. Two displayed the entero- hemolytic phenotype and were isolated from the carcass. Ten of the 18 potentially STEC isolates showed cytotoxic effect on Vero cells. Eight isolates from three different animals had the stx PCR amplicons sequenced. The translated sequences obtained from stx of three isolates from the carcass and of one isolate from feces corresponded to Stx2, showing 98% to 99% identity, with references at the GeneBank (NCBI). The sequences inferred from four amplicons obtained from isolates of faecal origin corresponded to Stx1, with 94% to 96% identity. The presence of STEC in the feces and carcasses of cattle at a slaughterhouse in the state of Minas Gerais emphasizes the importance of establishing a HACCP system and the need for implementation, evaluation, and validation of control measures to minimize the risk of cross-contamination during and after slaughter. |
publishDate |
2008 |
dc.date.issued.fl_str_mv |
2008-10-03 |
dc.date.accessioned.fl_str_mv |
2015-03-26T13:52:01Z |
dc.date.available.fl_str_mv |
2015-01-29 2015-03-26T13:52:01Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/masterThesis |
format |
masterThesis |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
GOMES, Andressa Pinheiro. Escherichia coli Shiga-Toxigênica (STEC) in slaughterhouse of bovines in the State of Minas Gerais, Brazil. 2008. 64 f. Dissertação (Mestrado em Associações micorrízicas; Bactérias láticas e probióticos; Biologia molecular de fungos de interesse) - Universidade Federal de Viçosa, Viçosa, 2008. |
dc.identifier.uri.fl_str_mv |
http://locus.ufv.br/handle/123456789/5376 |
identifier_str_mv |
GOMES, Andressa Pinheiro. Escherichia coli Shiga-Toxigênica (STEC) in slaughterhouse of bovines in the State of Minas Gerais, Brazil. 2008. 64 f. Dissertação (Mestrado em Associações micorrízicas; Bactérias láticas e probióticos; Biologia molecular de fungos de interesse) - Universidade Federal de Viçosa, Viçosa, 2008. |
url |
http://locus.ufv.br/handle/123456789/5376 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/embargoedAccess |
eu_rights_str_mv |
embargoedAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Universidade Federal de Viçosa |
dc.publisher.program.fl_str_mv |
Mestrado em Microbiologia Agrícola |
dc.publisher.initials.fl_str_mv |
UFV |
dc.publisher.country.fl_str_mv |
BR |
dc.publisher.department.fl_str_mv |
Associações micorrízicas; Bactérias láticas e probióticos; Biologia molecular de fungos de interesse |
publisher.none.fl_str_mv |
Universidade Federal de Viçosa |
dc.source.none.fl_str_mv |
reponame:LOCUS Repositório Institucional da UFV instname:Universidade Federal de Viçosa (UFV) instacron:UFV |
instname_str |
Universidade Federal de Viçosa (UFV) |
instacron_str |
UFV |
institution |
UFV |
reponame_str |
LOCUS Repositório Institucional da UFV |
collection |
LOCUS Repositório Institucional da UFV |
bitstream.url.fl_str_mv |
https://locus.ufv.br//bitstream/123456789/5376/1/texto%20completo.pdf https://locus.ufv.br//bitstream/123456789/5376/2/texto%20completo.pdf.txt https://locus.ufv.br//bitstream/123456789/5376/3/texto%20completo.pdf.jpg |
bitstream.checksum.fl_str_mv |
f0af95b88bc9afacbb9f1a952768a98e 3fb79b62c145728a73a0dad9b7a5cbc4 c12beacb20dbb15414fe21e10f2409bd |
bitstream.checksumAlgorithm.fl_str_mv |
MD5 MD5 MD5 |
repository.name.fl_str_mv |
LOCUS Repositório Institucional da UFV - Universidade Federal de Viçosa (UFV) |
repository.mail.fl_str_mv |
fabiojreis@ufv.br |
_version_ |
1801212990679154688 |