Expressão gênica em folículos e tecido ovariano durante o ciclo estral de suínos

• Autor(a) principal: Silva, Priscila Vendramini
• Data de Publicação: 2008
• Tipo de documento: Dissertação
• Idioma: por
• Título da fonte: LOCUS Repositório Institucional da UFV
• Texto Completo: http://locus.ufv.br/handle/123456789/4688
• topic: PCR em tempo real; Quantificação relativa; Foliculogênese; Real-time PCR; Relative quantification; Folliclegenesis; CNPQ::CIENCIAS AGRARIAS::ZOOTECNIA::GENETICA E MELHORAMENTO DOS ANIMAIS DOMESTICOS

During a regular estrous cycle, a network of hormonal events, which consist of hormones and growth factors, interact to regulate ovarian follicular growth through autocrine and paracrine mechanisms. These events are marked by a high dynamism of gene expression and level of transcripts in the ovary tissue. Therefore, the objective of the present study was to characterize the expression of genes IGF-I, IGFBP (1, 2, 3 e 5), EGF and genes related to follicular atresia (caspase 3 and p53) in follicle cells and ovary tissue in sows during 0, 6, 12 e 18 days of estrous cycle through real-time quantitative PCR (qPCR) technique. The total RNA was extracted from follicle cells and ovary tissue for each animal, and used to synthesize the first strand cDNA. The GAPDH gene was used as endogenous control. The results of gene expression were analyzed using linear regression with gene expression as dependent variable and days of estrous cycle as independent variables. The expression of each gene was detected on follicle cells and ovary tissue, IGFBP2 gene increased sequentially during the estrous cycle (P<0,02) and IGFBP3 gene shows a quadratic expression pattern (P<0,02). For others genes the level of expression did not change during estrous cycle. The qPCR showed to be efficient for detection of low levels of transcripts and leads to a better understanding of follicle development dynamics. Studies should be done to examine the expression of other related genes as well genes of other metabolic pathways in order to broaden our understanding about the follicular stages of folliculogenesis in the pig.