Membrana amniótica xenógena, associada ou não ao n-butil 2- cianoacrilato, no tratamento de lesão corneal perfurada. Estudo experimental em coelhos

• Autor(a) principal: Pontes, Kelly Cristine de Sousa
• Data de Publicação: 2010
• Tipo de documento: Tese
• Idioma: por
• Título da fonte: LOCUS Repositório Institucional da UFV
• Texto Completo: http://locus.ufv.br/handle/123456789/1438
• topic: Doença periodontal; Biomateriais; Regeneração tecidual aguada; Periodontal disease; Biomaterials; Acute tissue regeneration; CNPQ::CIENCIAS AGRARIAS::MEDICINA VETERINARIA::CLINICA E CIRURGIA ANIMAL

Researchs on surgical adhesives for the treatment of corneal perforations are many and show its effectiveness on maintaining ocular integrity and restraining inflammatory reaction. They show the adhesive`s properties favoring epithelization and its anti-inflammatory action. Thus, we compared the surgical techniques and the effects of N-butyl 2-cyanoacrylate and the bovine amniotic membrane (previously preserved in 99% glycerin under room temperature), aiming to offer technique for the repair of corneal perforations. For such, perforating keratectomies were made on the corneas of 60 rabbits equally divided in 4 groups. Group 1 (G1) was treated using N-butyl 2- cyanoacrylate applied on the lesions; group 2 (G2) received the amniotic membrane over the lesions and then the N-butyl 2-cyanoacrylate; in group 3 (G3), the same procedure as in group 2 was made, with additional coating with a amniotic membrane as a patch sutured to the corneal limbus; while group 4 (G4) was treated with keratoplasty with amniotic membrane covered by the same membrane patch, sutured to the corneal limbus. Daily ophthalmic examinations were made to verify parameters related to corneal, anterior chamber and lens condition, as well as corneal epithelization and the time during which the adhesive remained in place. Histopathology and histomorphometry were made at 2, 7, 15, 21 and 30 days post-operation (PO) for: the degree of corneal epithelization, leukocytes and fibroblasts presence, vessels presence, edema, and thickness of the epithelium and the stroma. Photophobia, blefarospasm, ocular discharge, conjunctival hyperemia and chemosis were observed, decreasing at 7 days PO. Anterior synechia occured in 2 animals in G1. Corneal edema occured in the perilesional area in all groups. Lens opacity was observed in G1, G2, and G3. In G2 and G3, the membrane acted as a barrier to the aqueous humour, keeping a dry surface to the application of the adhesive as well as preventing its contact with the intraocular structures and, when associated to the adhesive, it favored the repair process. The surgical technique associating the amniotic membrane to the N-butyl 2-cyanoacrylate was the easiest procedure to be implemented and more effective on the repair of perforated corneas in comparison to both the ones using only the adhesive or the membrane. Therefore, the association of the membrane to the adhesive can be indicated in the treatment of 2mm corneal perforations.