Aedes aegypti larvae treated with spinosad produce adults with damaged midgut and reduced fecundity
Autor(a) principal: | |
---|---|
Data de Publicação: | 2019 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | LOCUS Repositório Institucional da UFV |
Texto Completo: | https://doi.org/10.1016/j.chemosphere.2019.01.068 http://www.locus.ufv.br/handle/123456789/23924 |
Resumo: | The mosquito Aedes aegypti is the main vector of Dengue, Chikungunya, Zika, and yellow fever viruses, which are responsible for high human morbidity and mortality. The fight against these pathogens is mainly based on the control of the insect vector with the use of insecticides. Among insecticides, spinosad bioinsecticide is efficient against A. aegypti larvae and may be an alternative for vector control. Here, we investigate the sublethal effects of spinosad during midgut metamorphosis of A. aegypti females and its cumulative effects on blood acquisition capacity and fecundity in adults. We studied the midgut because it is an important model organ directly related to blood acquisition and digestion. Treatment of larvae with spinosad induced oxidative stress, apoptosis, and damage to the midgut cells at all stages of development and in adults. There was a reduction in the number of proliferating cells and the number of enteroendocrine cells in treated individuals. In addition, damage caused by spinosad led to a reduction in oviposition and egg viability of A. aegypti females. Finally, the exposure of mosquito larvae to sublethal concentrations of spinosad interfered with the development of the midgut, arresting the blood digestion and reproduction of adult females with blood digestion and reproduction difficulties. |
id |
UFV_fb6c0a700873c9719d78e339b71776f5 |
---|---|
oai_identifier_str |
oai:locus.ufv.br:123456789/23924 |
network_acronym_str |
UFV |
network_name_str |
LOCUS Repositório Institucional da UFV |
repository_id_str |
2145 |
spelling |
Fernandes, Kenner MoraisTomé, Hudson Vaner VenturaMiranda, Franciane RosaGonçalves, Wagner GonzagaPascini, Tales VicariSerrão, José EduardoMartins, Gustavo Ferreirapt-BR2019-03-13T18:20:52Z2019-03-13T18:20:52Z2019-040045-6535https://doi.org/10.1016/j.chemosphere.2019.01.068http://www.locus.ufv.br/handle/123456789/23924The mosquito Aedes aegypti is the main vector of Dengue, Chikungunya, Zika, and yellow fever viruses, which are responsible for high human morbidity and mortality. The fight against these pathogens is mainly based on the control of the insect vector with the use of insecticides. Among insecticides, spinosad bioinsecticide is efficient against A. aegypti larvae and may be an alternative for vector control. Here, we investigate the sublethal effects of spinosad during midgut metamorphosis of A. aegypti females and its cumulative effects on blood acquisition capacity and fecundity in adults. We studied the midgut because it is an important model organ directly related to blood acquisition and digestion. Treatment of larvae with spinosad induced oxidative stress, apoptosis, and damage to the midgut cells at all stages of development and in adults. There was a reduction in the number of proliferating cells and the number of enteroendocrine cells in treated individuals. In addition, damage caused by spinosad led to a reduction in oviposition and egg viability of A. aegypti females. Finally, the exposure of mosquito larvae to sublethal concentrations of spinosad interfered with the development of the midgut, arresting the blood digestion and reproduction of adult females with blood digestion and reproduction difficulties.engChemosphereVolume 221, Pages 464-470, April 2019Elsevier B. V.info:eu-repo/semantics/openAccessApoptosisBioinsecticideMetamorphosisMosquitoesInsecticide toxicityAedes aegypti larvae treated with spinosad produce adults with damaged midgut and reduced fecundityinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfreponame:LOCUS Repositório Institucional da UFVinstname:Universidade Federal de Viçosa (UFV)instacron:UFVORIGINALartigo.pdfartigo.pdfTexto completoapplication/pdf2711157https://locus.ufv.br//bitstream/123456789/23924/1/artigo.pdfb35bec8c3b0eb17f737f31abc7bc5724MD51LICENSElicense.txtlicense.txttext/plain; charset=utf-81748https://locus.ufv.br//bitstream/123456789/23924/2/license.txt8a4605be74aa9ea9d79846c1fba20a33MD52123456789/239242019-03-13 15:23:07.791oai:locus.ufv.br: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Repositório InstitucionalPUBhttps://www.locus.ufv.br/oai/requestfabiojreis@ufv.bropendoar:21452019-03-13T18:23:07LOCUS Repositório Institucional da UFV - Universidade Federal de Viçosa (UFV)false |
dc.title.en.fl_str_mv |
Aedes aegypti larvae treated with spinosad produce adults with damaged midgut and reduced fecundity |
title |
Aedes aegypti larvae treated with spinosad produce adults with damaged midgut and reduced fecundity |
spellingShingle |
Aedes aegypti larvae treated with spinosad produce adults with damaged midgut and reduced fecundity Fernandes, Kenner Morais Apoptosis Bioinsecticide Metamorphosis Mosquitoes Insecticide toxicity |
title_short |
Aedes aegypti larvae treated with spinosad produce adults with damaged midgut and reduced fecundity |
title_full |
Aedes aegypti larvae treated with spinosad produce adults with damaged midgut and reduced fecundity |
title_fullStr |
Aedes aegypti larvae treated with spinosad produce adults with damaged midgut and reduced fecundity |
title_full_unstemmed |
Aedes aegypti larvae treated with spinosad produce adults with damaged midgut and reduced fecundity |
title_sort |
Aedes aegypti larvae treated with spinosad produce adults with damaged midgut and reduced fecundity |
author |
Fernandes, Kenner Morais |
author_facet |
Fernandes, Kenner Morais Tomé, Hudson Vaner Ventura Miranda, Franciane Rosa Gonçalves, Wagner Gonzaga Pascini, Tales Vicari Serrão, José Eduardo Martins, Gustavo Ferreirapt-BR |
author_role |
author |
author2 |
Tomé, Hudson Vaner Ventura Miranda, Franciane Rosa Gonçalves, Wagner Gonzaga Pascini, Tales Vicari Serrão, José Eduardo Martins, Gustavo Ferreirapt-BR |
author2_role |
author author author author author author |
dc.contributor.author.fl_str_mv |
Fernandes, Kenner Morais Tomé, Hudson Vaner Ventura Miranda, Franciane Rosa Gonçalves, Wagner Gonzaga Pascini, Tales Vicari Serrão, José Eduardo Martins, Gustavo Ferreirapt-BR |
dc.subject.pt-BR.fl_str_mv |
Apoptosis Bioinsecticide Metamorphosis Mosquitoes Insecticide toxicity |
topic |
Apoptosis Bioinsecticide Metamorphosis Mosquitoes Insecticide toxicity |
description |
The mosquito Aedes aegypti is the main vector of Dengue, Chikungunya, Zika, and yellow fever viruses, which are responsible for high human morbidity and mortality. The fight against these pathogens is mainly based on the control of the insect vector with the use of insecticides. Among insecticides, spinosad bioinsecticide is efficient against A. aegypti larvae and may be an alternative for vector control. Here, we investigate the sublethal effects of spinosad during midgut metamorphosis of A. aegypti females and its cumulative effects on blood acquisition capacity and fecundity in adults. We studied the midgut because it is an important model organ directly related to blood acquisition and digestion. Treatment of larvae with spinosad induced oxidative stress, apoptosis, and damage to the midgut cells at all stages of development and in adults. There was a reduction in the number of proliferating cells and the number of enteroendocrine cells in treated individuals. In addition, damage caused by spinosad led to a reduction in oviposition and egg viability of A. aegypti females. Finally, the exposure of mosquito larvae to sublethal concentrations of spinosad interfered with the development of the midgut, arresting the blood digestion and reproduction of adult females with blood digestion and reproduction difficulties. |
publishDate |
2019 |
dc.date.accessioned.fl_str_mv |
2019-03-13T18:20:52Z |
dc.date.available.fl_str_mv |
2019-03-13T18:20:52Z |
dc.date.issued.fl_str_mv |
2019-04 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://doi.org/10.1016/j.chemosphere.2019.01.068 http://www.locus.ufv.br/handle/123456789/23924 |
dc.identifier.issn.none.fl_str_mv |
0045-6535 |
identifier_str_mv |
0045-6535 |
url |
https://doi.org/10.1016/j.chemosphere.2019.01.068 http://www.locus.ufv.br/handle/123456789/23924 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.ispartofseries.pt-BR.fl_str_mv |
Volume 221, Pages 464-470, April 2019 |
dc.rights.driver.fl_str_mv |
Elsevier B. V. info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Elsevier B. V. |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Chemosphere |
publisher.none.fl_str_mv |
Chemosphere |
dc.source.none.fl_str_mv |
reponame:LOCUS Repositório Institucional da UFV instname:Universidade Federal de Viçosa (UFV) instacron:UFV |
instname_str |
Universidade Federal de Viçosa (UFV) |
instacron_str |
UFV |
institution |
UFV |
reponame_str |
LOCUS Repositório Institucional da UFV |
collection |
LOCUS Repositório Institucional da UFV |
bitstream.url.fl_str_mv |
https://locus.ufv.br//bitstream/123456789/23924/1/artigo.pdf https://locus.ufv.br//bitstream/123456789/23924/2/license.txt |
bitstream.checksum.fl_str_mv |
b35bec8c3b0eb17f737f31abc7bc5724 8a4605be74aa9ea9d79846c1fba20a33 |
bitstream.checksumAlgorithm.fl_str_mv |
MD5 MD5 |
repository.name.fl_str_mv |
LOCUS Repositório Institucional da UFV - Universidade Federal de Viçosa (UFV) |
repository.mail.fl_str_mv |
fabiojreis@ufv.br |
_version_ |
1801212998870630400 |