Citotoxicidade de três cimentos de uso endodôntico em células L 929
Autor(a) principal: | |
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Data de Publicação: | 2007 |
Tipo de documento: | Dissertação |
Idioma: | por |
Título da fonte: | Biblioteca Digital de Teses e Dissertações da UNIGRANRIO |
Texto Completo: | http://localhost:8080/tede/handle/tede/108 |
Resumo: | The procedure of this in vitro research based itself on the evaluation of the cytotoxicity of three sealers of endodontic use, epoxic resin base: Sealer 26®, AH Plus® and Acroseal®. To such an experiment, after 48 h of manipulation were accomplished by the agar diffusion methodology associated to the red neutral colorant on cells L-929, trough the seeding in appliance of six cavities. After 24 hour in a heater humidificated at 37ºC with 5% of CO2, a clear halo could be seen around the samples and the positive controls, elating to the specimens cytotoxicity of the sealers. The measurement was made according to Operational Protocol Standard POP/FIOCRUZ. All the sealers presented cytotoxicity, but the AH PLUS® sealer showed the best biological behavior, estimating the lowest cytotoxicity index followed by the Sealer 26® and Acroseal® which presented the worse index of biological reactivity. The statistics data were estimated by the application of ANOVA and Student-Newman tests, p<0,05. |
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Fidel, Sandra RiveraFidel, Sandra RiveraBruno, Adília Maria VieiraKalil , Marcos da VeigaPinheiro, Rosangela Acris2016-11-25T11:59:57Z2007-04-19PINHEIRO, Rosangela Acris. Citotoxicidade de três cimentos de uso endodôntico em células L 929. 2007. 101 f. Dissertação (Mestrado em Odontologia) - Universidade do Grande Rio Prof. José de Souza Herdy", Duque de Caxias.http://localhost:8080/tede/handle/tede/108The procedure of this in vitro research based itself on the evaluation of the cytotoxicity of three sealers of endodontic use, epoxic resin base: Sealer 26®, AH Plus® and Acroseal®. To such an experiment, after 48 h of manipulation were accomplished by the agar diffusion methodology associated to the red neutral colorant on cells L-929, trough the seeding in appliance of six cavities. After 24 hour in a heater humidificated at 37ºC with 5% of CO2, a clear halo could be seen around the samples and the positive controls, elating to the specimens cytotoxicity of the sealers. The measurement was made according to Operational Protocol Standard POP/FIOCRUZ. All the sealers presented cytotoxicity, but the AH PLUS® sealer showed the best biological behavior, estimating the lowest cytotoxicity index followed by the Sealer 26® and Acroseal® which presented the worse index of biological reactivity. The statistics data were estimated by the application of ANOVA and Student-Newman tests, p<0,05.A pesquisa in vitro abordada no presente trabalho baseou-se na avaliação da citotoxicidade de três cimentos de uso endodôntico contendo resina epóxica: Sealer 26®, AH Plus® e Acroseal®. Para tal experimento, foi realizado o ensaio de difusão em angar com o corante vermelho neutro sobre celulas L-929, através semeadura em placas de 6 poços, após 48 horas da manipulação. Transcorridas 24 h em uma estufa umidificada à 37º C com 5% de CO², foi constatado a formação de um halo claro ao redor das amostras e dos controles positivos, relativos a citotoxicidade dos espécimes dos cimentos. A medição foi realizada por quadrante, de acordo com o Protocolo Operacional Padrão (POP/FIOCRUZ). Todos os cimentos acima mencionados, apresentaram citotoxicidade, sendo que o cimento AH PLUS® obteve o melhor comportamento biológico, aferindo menor citotoxidade, seguido dos cimentos Sealer 26® e Acroseal®. Os dados estatísticos foram aferidos com a aplicação dos testes ANOVA e Student-Newman-Keuls, p<0,05.Submitted by Janser dos Santos Nascimento (janser.nascimento@unigranrio.com.br) on 2016-11-25T11:59:57Z No. of bitstreams: 2 license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) Rosangela Acris Pinheiro.pdf: 50695911 bytes, checksum: 7aa45a9ce8f83085387a84026ba2e13a (MD5)Made available in DSpace on 2016-11-25T11:59:57Z (GMT). No. of bitstreams: 2 license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) Rosangela Acris Pinheiro.pdf: 50695911 bytes, checksum: 7aa45a9ce8f83085387a84026ba2e13a (MD5) Previous issue date: 2007-04-19CNPqapplication/pdfporUniversidade do Grande RioPrograma de Pós-Graduação em OdontologiaUNIGRANRIOBrasilUnigranrio::Odontologiahttp://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccessOdontologiaEndodontiaCimentos dentáriosODONTOLOGIA::ENDONTIACitotoxicidade de três cimentos de uso endodôntico em células L 929info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisreponame:Biblioteca Digital de Teses e Dissertações da UNIGRANRIOinstname:Universidade do Grande Rio (UNIGRANRIO)instacron:UNIGRANRIOLICENSElicense.txtlicense.txttext/plain; charset=utf-81982http://localhost:8080/tede/bitstream/tede/108/1/license.txt4a50535e8405f611398e6e2d408dbd1bMD51CC-LICENSElicense_urllicense_urltext/plain; charset=utf-849http://localhost:8080/tede/bitstream/tede/108/2/license_url4afdbb8c545fd630ea7db775da747b2fMD52license_textlicense_texttext/html; charset=utf-80http://localhost:8080/tede/bitstream/tede/108/3/license_textd41d8cd98f00b204e9800998ecf8427eMD53license_rdflicense_rdfapplication/rdf+xml; charset=utf-80http://localhost:8080/tede/bitstream/tede/108/4/license_rdfd41d8cd98f00b204e9800998ecf8427eMD54ORIGINALRosangela Acris Pinheiro.pdfRosangela Acris Pinheiro.pdfapplication/pdf50695911http://localhost:8080/tede/bitstream/tede/108/5/Rosangela+Acris+Pinheiro.pdf7aa45a9ce8f83085387a84026ba2e13aMD55tede/1082016-11-25 09:59:57.996oai:localhost: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Biblioteca Digital de Teses e Dissertaçõeshttp://tede.unigranrio.edu.br/PUBhttp://tede.unigranrio.edu.br/oai/requestrepositorio@instituicao.br||repositorio@instituicao.bropendoar:2016-11-25T11:59:57Biblioteca Digital de Teses e Dissertações da UNIGRANRIO - Universidade do Grande Rio (UNIGRANRIO)false |
dc.title.por.fl_str_mv |
Citotoxicidade de três cimentos de uso endodôntico em células L 929 |
title |
Citotoxicidade de três cimentos de uso endodôntico em células L 929 |
spellingShingle |
Citotoxicidade de três cimentos de uso endodôntico em células L 929 Pinheiro, Rosangela Acris Odontologia Endodontia Cimentos dentários ODONTOLOGIA::ENDONTIA |
title_short |
Citotoxicidade de três cimentos de uso endodôntico em células L 929 |
title_full |
Citotoxicidade de três cimentos de uso endodôntico em células L 929 |
title_fullStr |
Citotoxicidade de três cimentos de uso endodôntico em células L 929 |
title_full_unstemmed |
Citotoxicidade de três cimentos de uso endodôntico em células L 929 |
title_sort |
Citotoxicidade de três cimentos de uso endodôntico em células L 929 |
author |
Pinheiro, Rosangela Acris |
author_facet |
Pinheiro, Rosangela Acris |
author_role |
author |
dc.contributor.advisor1.fl_str_mv |
Fidel, Sandra Rivera |
dc.contributor.referee1.fl_str_mv |
Fidel, Sandra Rivera |
dc.contributor.referee2.fl_str_mv |
Bruno, Adília Maria Vieira |
dc.contributor.referee3.fl_str_mv |
Kalil , Marcos da Veiga |
dc.contributor.author.fl_str_mv |
Pinheiro, Rosangela Acris |
contributor_str_mv |
Fidel, Sandra Rivera Fidel, Sandra Rivera Bruno, Adília Maria Vieira Kalil , Marcos da Veiga |
dc.subject.por.fl_str_mv |
Odontologia Endodontia Cimentos dentários |
topic |
Odontologia Endodontia Cimentos dentários ODONTOLOGIA::ENDONTIA |
dc.subject.cnpq.fl_str_mv |
ODONTOLOGIA::ENDONTIA |
description |
The procedure of this in vitro research based itself on the evaluation of the cytotoxicity of three sealers of endodontic use, epoxic resin base: Sealer 26®, AH Plus® and Acroseal®. To such an experiment, after 48 h of manipulation were accomplished by the agar diffusion methodology associated to the red neutral colorant on cells L-929, trough the seeding in appliance of six cavities. After 24 hour in a heater humidificated at 37ºC with 5% of CO2, a clear halo could be seen around the samples and the positive controls, elating to the specimens cytotoxicity of the sealers. The measurement was made according to Operational Protocol Standard POP/FIOCRUZ. All the sealers presented cytotoxicity, but the AH PLUS® sealer showed the best biological behavior, estimating the lowest cytotoxicity index followed by the Sealer 26® and Acroseal® which presented the worse index of biological reactivity. The statistics data were estimated by the application of ANOVA and Student-Newman tests, p<0,05. |
publishDate |
2007 |
dc.date.issued.fl_str_mv |
2007-04-19 |
dc.date.accessioned.fl_str_mv |
2016-11-25T11:59:57Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/masterThesis |
format |
masterThesis |
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publishedVersion |
dc.identifier.citation.fl_str_mv |
PINHEIRO, Rosangela Acris. Citotoxicidade de três cimentos de uso endodôntico em células L 929. 2007. 101 f. Dissertação (Mestrado em Odontologia) - Universidade do Grande Rio Prof. José de Souza Herdy", Duque de Caxias. |
dc.identifier.uri.fl_str_mv |
http://localhost:8080/tede/handle/tede/108 |
identifier_str_mv |
PINHEIRO, Rosangela Acris. Citotoxicidade de três cimentos de uso endodôntico em células L 929. 2007. 101 f. Dissertação (Mestrado em Odontologia) - Universidade do Grande Rio Prof. José de Souza Herdy", Duque de Caxias. |
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http://localhost:8080/tede/handle/tede/108 |
dc.language.iso.fl_str_mv |
por |
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por |
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http://creativecommons.org/licenses/by-nc-nd/4.0/ info:eu-repo/semantics/openAccess |
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http://creativecommons.org/licenses/by-nc-nd/4.0/ |
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openAccess |
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application/pdf |
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Universidade do Grande Rio |
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Programa de Pós-Graduação em Odontologia |
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UNIGRANRIO |
dc.publisher.country.fl_str_mv |
Brasil |
dc.publisher.department.fl_str_mv |
Unigranrio::Odontologia |
publisher.none.fl_str_mv |
Universidade do Grande Rio |
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