Mutagenicity induced by the hydroalcoholic extract of the medicinal plant Plathymenia reticulata Benth
Autor(a) principal: | |
---|---|
Data de Publicação: | 2011 |
Outros Autores: | , , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | The Journal of venomous animals and toxins including tropical diseases (Online) |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992011000200010 |
Resumo: | Plathymenia reticulata Benth has an anti-inflammatory effect and is capable of neutralizing the neuromuscular blockade induced by Bothrops jararacussu or Crotalus durissus terrificus venoms, probably by precipitating venom proteins (an effect caused by plant tannins). The present study aimed to evaluate the mutagenic activity of P. reticulata by using the Salmonella mutagenicity assay (Ames test) and the micronucleus test in CHO-K1 cells. P. reticulata extract concentrations of 2.84, 5.68, 11.37, and 19.90 mg/plate were assayed by the Ames test using TA97a, TA98, TA100 and TA102 bacterial strains, with (+S9) and without (-S9) metabolic activation. Concentrations of 5, 1.6 and 0.5 μg/mL of P. reticulata extract were used for the micronucleus test. P. reticulata extract was mutagenic to TA98 (-S9) and showed signs of mutagenic activity in TA97a and TA102 (both -S9) strains. Micronucleus test CBPI values showed that the endogenous metabolic system increased the number of viable cells when compared to the non-activated samples and the micronucleus frequency increased when the cells were treated in the absence of S9. We concluded that P. reticulata extract may present direct mutagenic properties. |
id |
UNESP-11_34cf38c91d52d9ecdf220322454dfb8f |
---|---|
oai_identifier_str |
oai:scielo:S1678-91992011000200010 |
network_acronym_str |
UNESP-11 |
network_name_str |
The Journal of venomous animals and toxins including tropical diseases (Online) |
repository_id_str |
|
spelling |
Mutagenicity induced by the hydroalcoholic extract of the medicinal plant Plathymenia reticulata BenthAmes testmicronucleus testmutagenicityplant extractPlathymenia reticulataPlathymenia reticulata Benth has an anti-inflammatory effect and is capable of neutralizing the neuromuscular blockade induced by Bothrops jararacussu or Crotalus durissus terrificus venoms, probably by precipitating venom proteins (an effect caused by plant tannins). The present study aimed to evaluate the mutagenic activity of P. reticulata by using the Salmonella mutagenicity assay (Ames test) and the micronucleus test in CHO-K1 cells. P. reticulata extract concentrations of 2.84, 5.68, 11.37, and 19.90 mg/plate were assayed by the Ames test using TA97a, TA98, TA100 and TA102 bacterial strains, with (+S9) and without (-S9) metabolic activation. Concentrations of 5, 1.6 and 0.5 μg/mL of P. reticulata extract were used for the micronucleus test. P. reticulata extract was mutagenic to TA98 (-S9) and showed signs of mutagenic activity in TA97a and TA102 (both -S9) strains. Micronucleus test CBPI values showed that the endogenous metabolic system increased the number of viable cells when compared to the non-activated samples and the micronucleus frequency increased when the cells were treated in the absence of S9. We concluded that P. reticulata extract may present direct mutagenic properties.Centro de Estudos de Venenos e Animais Peçonhentos (CEVAP/UNESP)2011-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992011000200010Journal of Venomous Animals and Toxins including Tropical Diseases v.17 n.2 2011reponame:The Journal of venomous animals and toxins including tropical diseases (Online)instname:Universidade Estadual Paulista (UNESP)instacron:UNESP10.1590/S1678-91992011000200010info:eu-repo/semantics/openAccessDella Torre,AAlbuquerque,LBLFarrapo,NMOshima-Franco,YSantos,MGTavares,RVSRodas,ACDDal Belo,CACardoso,CRPVaranda,EAGroppo,FCLopes,PSeng2011-12-06T00:00:00Zoai:scielo:S1678-91992011000200010Revistahttp://www.scielo.br/jvatitdPUBhttps://old.scielo.br/oai/scielo-oai.php||editorial@jvat.org.br1678-91991678-9180opendoar:2011-12-06T00:00The Journal of venomous animals and toxins including tropical diseases (Online) - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Mutagenicity induced by the hydroalcoholic extract of the medicinal plant Plathymenia reticulata Benth |
title |
Mutagenicity induced by the hydroalcoholic extract of the medicinal plant Plathymenia reticulata Benth |
spellingShingle |
Mutagenicity induced by the hydroalcoholic extract of the medicinal plant Plathymenia reticulata Benth Della Torre,A Ames test micronucleus test mutagenicity plant extract Plathymenia reticulata |
title_short |
Mutagenicity induced by the hydroalcoholic extract of the medicinal plant Plathymenia reticulata Benth |
title_full |
Mutagenicity induced by the hydroalcoholic extract of the medicinal plant Plathymenia reticulata Benth |
title_fullStr |
Mutagenicity induced by the hydroalcoholic extract of the medicinal plant Plathymenia reticulata Benth |
title_full_unstemmed |
Mutagenicity induced by the hydroalcoholic extract of the medicinal plant Plathymenia reticulata Benth |
title_sort |
Mutagenicity induced by the hydroalcoholic extract of the medicinal plant Plathymenia reticulata Benth |
author |
Della Torre,A |
author_facet |
Della Torre,A Albuquerque,LBL Farrapo,NM Oshima-Franco,Y Santos,MG Tavares,RVS Rodas,ACD Dal Belo,CA Cardoso,CRP Varanda,EA Groppo,FC Lopes,PS |
author_role |
author |
author2 |
Albuquerque,LBL Farrapo,NM Oshima-Franco,Y Santos,MG Tavares,RVS Rodas,ACD Dal Belo,CA Cardoso,CRP Varanda,EA Groppo,FC Lopes,PS |
author2_role |
author author author author author author author author author author author |
dc.contributor.author.fl_str_mv |
Della Torre,A Albuquerque,LBL Farrapo,NM Oshima-Franco,Y Santos,MG Tavares,RVS Rodas,ACD Dal Belo,CA Cardoso,CRP Varanda,EA Groppo,FC Lopes,PS |
dc.subject.por.fl_str_mv |
Ames test micronucleus test mutagenicity plant extract Plathymenia reticulata |
topic |
Ames test micronucleus test mutagenicity plant extract Plathymenia reticulata |
description |
Plathymenia reticulata Benth has an anti-inflammatory effect and is capable of neutralizing the neuromuscular blockade induced by Bothrops jararacussu or Crotalus durissus terrificus venoms, probably by precipitating venom proteins (an effect caused by plant tannins). The present study aimed to evaluate the mutagenic activity of P. reticulata by using the Salmonella mutagenicity assay (Ames test) and the micronucleus test in CHO-K1 cells. P. reticulata extract concentrations of 2.84, 5.68, 11.37, and 19.90 mg/plate were assayed by the Ames test using TA97a, TA98, TA100 and TA102 bacterial strains, with (+S9) and without (-S9) metabolic activation. Concentrations of 5, 1.6 and 0.5 μg/mL of P. reticulata extract were used for the micronucleus test. P. reticulata extract was mutagenic to TA98 (-S9) and showed signs of mutagenic activity in TA97a and TA102 (both -S9) strains. Micronucleus test CBPI values showed that the endogenous metabolic system increased the number of viable cells when compared to the non-activated samples and the micronucleus frequency increased when the cells were treated in the absence of S9. We concluded that P. reticulata extract may present direct mutagenic properties. |
publishDate |
2011 |
dc.date.none.fl_str_mv |
2011-01-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992011000200010 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992011000200010 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S1678-91992011000200010 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Centro de Estudos de Venenos e Animais Peçonhentos (CEVAP/UNESP) |
publisher.none.fl_str_mv |
Centro de Estudos de Venenos e Animais Peçonhentos (CEVAP/UNESP) |
dc.source.none.fl_str_mv |
Journal of Venomous Animals and Toxins including Tropical Diseases v.17 n.2 2011 reponame:The Journal of venomous animals and toxins including tropical diseases (Online) instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
The Journal of venomous animals and toxins including tropical diseases (Online) |
collection |
The Journal of venomous animals and toxins including tropical diseases (Online) |
repository.name.fl_str_mv |
The Journal of venomous animals and toxins including tropical diseases (Online) - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
||editorial@jvat.org.br |
_version_ |
1748958539130339328 |