Cell-mediated immune response to Leishmania chagasi experimental infection of BALB/c immunosuppressed mice
Autor(a) principal: | |
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Data de Publicação: | 2010 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | The Journal of venomous animals and toxins including tropical diseases (Online) |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992010000100013 |
Resumo: | Leishmaniasis, a zoonosis of worldwide distribution, presents a significant impact on immunosupressed patients. This study aimed to evaluate Leishmania chagasi infection in BALB/c mice immunosuppressed with dexamethasone. Spleen cells stimulated or not with L. chagasi were cultured for cytokine quantification (IFN-γ, IL-2, IL-4 and IL-10) by sandwich ELISA. Parasite loads in the spleen and liver were determined by means of culture microtitration. Immunosuppressed groups showed statistically lower spleen weight and CD4-cell percentage in blood on the day of infection and produced Th1 and Th2 cytokines on other days of the study. The other infected groups, weather immunosupressed or not, also produced Th1 and Th2 cytokines. Parasite loads in the spleen and liver were not statistically different among the groups. It was concluded that L. chagasi infection was not affected by dexamethasone-induced immunosuppression, probably due the reversible effect of the treatment. |
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The Journal of venomous animals and toxins including tropical diseases (Online) |
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Cell-mediated immune response to Leishmania chagasi experimental infection of BALB/c immunosuppressed micevisceral leishmaniasisimmunosuppressionimmunopathologymiceinterleukinsLeishmaniasis, a zoonosis of worldwide distribution, presents a significant impact on immunosupressed patients. This study aimed to evaluate Leishmania chagasi infection in BALB/c mice immunosuppressed with dexamethasone. Spleen cells stimulated or not with L. chagasi were cultured for cytokine quantification (IFN-γ, IL-2, IL-4 and IL-10) by sandwich ELISA. Parasite loads in the spleen and liver were determined by means of culture microtitration. Immunosuppressed groups showed statistically lower spleen weight and CD4-cell percentage in blood on the day of infection and produced Th1 and Th2 cytokines on other days of the study. The other infected groups, weather immunosupressed or not, also produced Th1 and Th2 cytokines. Parasite loads in the spleen and liver were not statistically different among the groups. It was concluded that L. chagasi infection was not affected by dexamethasone-induced immunosuppression, probably due the reversible effect of the treatment.Centro de Estudos de Venenos e Animais Peçonhentos (CEVAP/UNESP)2010-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992010000100013Journal of Venomous Animals and Toxins including Tropical Diseases v.16 n.1 2010reponame:The Journal of venomous animals and toxins including tropical diseases (Online)instname:Universidade Estadual Paulista (UNESP)instacron:UNESP10.1590/S1678-91992010000100013info:eu-repo/semantics/openAccessMachado,JGHoffmann,JLKrause,VLKda Silva,AVDias-Melicio,LALangoni,Heng2010-03-18T00:00:00Zoai:scielo:S1678-91992010000100013Revistahttp://www.scielo.br/jvatitdPUBhttps://old.scielo.br/oai/scielo-oai.php||editorial@jvat.org.br1678-91991678-9180opendoar:2010-03-18T00:00The Journal of venomous animals and toxins including tropical diseases (Online) - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Cell-mediated immune response to Leishmania chagasi experimental infection of BALB/c immunosuppressed mice |
title |
Cell-mediated immune response to Leishmania chagasi experimental infection of BALB/c immunosuppressed mice |
spellingShingle |
Cell-mediated immune response to Leishmania chagasi experimental infection of BALB/c immunosuppressed mice Machado,JG visceral leishmaniasis immunosuppression immunopathology mice interleukins |
title_short |
Cell-mediated immune response to Leishmania chagasi experimental infection of BALB/c immunosuppressed mice |
title_full |
Cell-mediated immune response to Leishmania chagasi experimental infection of BALB/c immunosuppressed mice |
title_fullStr |
Cell-mediated immune response to Leishmania chagasi experimental infection of BALB/c immunosuppressed mice |
title_full_unstemmed |
Cell-mediated immune response to Leishmania chagasi experimental infection of BALB/c immunosuppressed mice |
title_sort |
Cell-mediated immune response to Leishmania chagasi experimental infection of BALB/c immunosuppressed mice |
author |
Machado,JG |
author_facet |
Machado,JG Hoffmann,JL Krause,VLK da Silva,AV Dias-Melicio,LA Langoni,H |
author_role |
author |
author2 |
Hoffmann,JL Krause,VLK da Silva,AV Dias-Melicio,LA Langoni,H |
author2_role |
author author author author author |
dc.contributor.author.fl_str_mv |
Machado,JG Hoffmann,JL Krause,VLK da Silva,AV Dias-Melicio,LA Langoni,H |
dc.subject.por.fl_str_mv |
visceral leishmaniasis immunosuppression immunopathology mice interleukins |
topic |
visceral leishmaniasis immunosuppression immunopathology mice interleukins |
description |
Leishmaniasis, a zoonosis of worldwide distribution, presents a significant impact on immunosupressed patients. This study aimed to evaluate Leishmania chagasi infection in BALB/c mice immunosuppressed with dexamethasone. Spleen cells stimulated or not with L. chagasi were cultured for cytokine quantification (IFN-γ, IL-2, IL-4 and IL-10) by sandwich ELISA. Parasite loads in the spleen and liver were determined by means of culture microtitration. Immunosuppressed groups showed statistically lower spleen weight and CD4-cell percentage in blood on the day of infection and produced Th1 and Th2 cytokines on other days of the study. The other infected groups, weather immunosupressed or not, also produced Th1 and Th2 cytokines. Parasite loads in the spleen and liver were not statistically different among the groups. It was concluded that L. chagasi infection was not affected by dexamethasone-induced immunosuppression, probably due the reversible effect of the treatment. |
publishDate |
2010 |
dc.date.none.fl_str_mv |
2010-01-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992010000100013 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992010000100013 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S1678-91992010000100013 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Centro de Estudos de Venenos e Animais Peçonhentos (CEVAP/UNESP) |
publisher.none.fl_str_mv |
Centro de Estudos de Venenos e Animais Peçonhentos (CEVAP/UNESP) |
dc.source.none.fl_str_mv |
Journal of Venomous Animals and Toxins including Tropical Diseases v.16 n.1 2010 reponame:The Journal of venomous animals and toxins including tropical diseases (Online) instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
The Journal of venomous animals and toxins including tropical diseases (Online) |
collection |
The Journal of venomous animals and toxins including tropical diseases (Online) |
repository.name.fl_str_mv |
The Journal of venomous animals and toxins including tropical diseases (Online) - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
||editorial@jvat.org.br |
_version_ |
1748958538740269056 |