Construction of a recombinant plasmid pSH-G containing the rabies-virus glycoprotein G gene

Detalhes bibliográficos
Autor(a) principal: Carnieli JR,P.
Data de Publicação: 2007
Outros Autores: Ventura,A. M., Durigon,E. L.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: The Journal of venomous animals and toxins including tropical diseases (Online)
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992007000400015
Resumo: A plasmid named pSH-G was constructed with the rabies-virus G-gene insert. This plasmid was transfected into eukaryotic BHK-21 cells and its stability tested. The presence of the pSH-G plasmid was confirmed by means of polymerase chain reaction (PCR) after each of ten cell passages, and the results were positive. The stable BHK-21/pSH-G+ clone obtained can be used in the study of rabies as well as in the production of vaccines.
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spelling Construction of a recombinant plasmid pSH-G containing the rabies-virus glycoprotein G generabiesG geneplasmidstransfectionA plasmid named pSH-G was constructed with the rabies-virus G-gene insert. This plasmid was transfected into eukaryotic BHK-21 cells and its stability tested. The presence of the pSH-G plasmid was confirmed by means of polymerase chain reaction (PCR) after each of ten cell passages, and the results were positive. The stable BHK-21/pSH-G+ clone obtained can be used in the study of rabies as well as in the production of vaccines.Centro de Estudos de Venenos e Animais Peçonhentos (CEVAP/UNESP)2007-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992007000400015Journal of Venomous Animals and Toxins including Tropical Diseases v.13 n.4 2007reponame:The Journal of venomous animals and toxins including tropical diseases (Online)instname:Universidade Estadual Paulista (UNESP)instacron:UNESP10.1590/S1678-91992007000400015info:eu-repo/semantics/openAccessCarnieli JR,P.Ventura,A. M.Durigon,E. L.eng2007-12-19T00:00:00Zoai:scielo:S1678-91992007000400015Revistahttp://www.scielo.br/jvatitdPUBhttps://old.scielo.br/oai/scielo-oai.php||editorial@jvat.org.br1678-91991678-9180opendoar:2007-12-19T00:00The Journal of venomous animals and toxins including tropical diseases (Online) - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Construction of a recombinant plasmid pSH-G containing the rabies-virus glycoprotein G gene
title Construction of a recombinant plasmid pSH-G containing the rabies-virus glycoprotein G gene
spellingShingle Construction of a recombinant plasmid pSH-G containing the rabies-virus glycoprotein G gene
Carnieli JR,P.
rabies
G gene
plasmids
transfection
title_short Construction of a recombinant plasmid pSH-G containing the rabies-virus glycoprotein G gene
title_full Construction of a recombinant plasmid pSH-G containing the rabies-virus glycoprotein G gene
title_fullStr Construction of a recombinant plasmid pSH-G containing the rabies-virus glycoprotein G gene
title_full_unstemmed Construction of a recombinant plasmid pSH-G containing the rabies-virus glycoprotein G gene
title_sort Construction of a recombinant plasmid pSH-G containing the rabies-virus glycoprotein G gene
author Carnieli JR,P.
author_facet Carnieli JR,P.
Ventura,A. M.
Durigon,E. L.
author_role author
author2 Ventura,A. M.
Durigon,E. L.
author2_role author
author
dc.contributor.author.fl_str_mv Carnieli JR,P.
Ventura,A. M.
Durigon,E. L.
dc.subject.por.fl_str_mv rabies
G gene
plasmids
transfection
topic rabies
G gene
plasmids
transfection
description A plasmid named pSH-G was constructed with the rabies-virus G-gene insert. This plasmid was transfected into eukaryotic BHK-21 cells and its stability tested. The presence of the pSH-G plasmid was confirmed by means of polymerase chain reaction (PCR) after each of ten cell passages, and the results were positive. The stable BHK-21/pSH-G+ clone obtained can be used in the study of rabies as well as in the production of vaccines.
publishDate 2007
dc.date.none.fl_str_mv 2007-01-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992007000400015
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992007000400015
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S1678-91992007000400015
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Centro de Estudos de Venenos e Animais Peçonhentos (CEVAP/UNESP)
publisher.none.fl_str_mv Centro de Estudos de Venenos e Animais Peçonhentos (CEVAP/UNESP)
dc.source.none.fl_str_mv Journal of Venomous Animals and Toxins including Tropical Diseases v.13 n.4 2007
reponame:The Journal of venomous animals and toxins including tropical diseases (Online)
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str The Journal of venomous animals and toxins including tropical diseases (Online)
collection The Journal of venomous animals and toxins including tropical diseases (Online)
repository.name.fl_str_mv The Journal of venomous animals and toxins including tropical diseases (Online) - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv ||editorial@jvat.org.br
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