Expression of schistosomal cathepsin l1 in Escherichia coli and evaluation of its protective capacity in an animal challenge
Autor(a) principal: | |
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Data de Publicação: | 2009 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | The Journal of venomous animals and toxins including tropical diseases (Online) |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992009000200011 |
Resumo: | Schistosomes utilize proteinases to accomplish several activities such as tissue penetration, tissue digestion and evasion of host immune responses. Cathepsin L is a cysteine proteinase of the papain superfamily detected in their gut lumen which indicates that this enzyme contributes to the proteolysis of ingested hemoglobin. Due to the roles played in the schistosome biology, proteolytic enzymes are considered potential targets for developing and guiding antischistosomal therapies. In the present work, the cathepsin L1 cDNA coding of Schistosoma mansoni was cloned into the pAE vector that provides high-level expression of heterologous proteins in Escherichia coli. The recombinant protein was expressed as inclusion bodies, purified under denaturing conditions through nickel-charged chromatography and used for experimental animal vaccination. ELISA was performed with the pooled sera. Although this protein was shown to be immunogenic, mice immunized with three doses of recombinant protein plus aluminum hydroxide as adjuvant were not protected against S. mansoni infection. |
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The Journal of venomous animals and toxins including tropical diseases (Online) |
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spelling |
Expression of schistosomal cathepsin l1 in Escherichia coli and evaluation of its protective capacity in an animal challengeSchistosoma mansonicysteine proteinasecathepsin L1vaccineimmunogenicitySchistosomes utilize proteinases to accomplish several activities such as tissue penetration, tissue digestion and evasion of host immune responses. Cathepsin L is a cysteine proteinase of the papain superfamily detected in their gut lumen which indicates that this enzyme contributes to the proteolysis of ingested hemoglobin. Due to the roles played in the schistosome biology, proteolytic enzymes are considered potential targets for developing and guiding antischistosomal therapies. In the present work, the cathepsin L1 cDNA coding of Schistosoma mansoni was cloned into the pAE vector that provides high-level expression of heterologous proteins in Escherichia coli. The recombinant protein was expressed as inclusion bodies, purified under denaturing conditions through nickel-charged chromatography and used for experimental animal vaccination. ELISA was performed with the pooled sera. Although this protein was shown to be immunogenic, mice immunized with three doses of recombinant protein plus aluminum hydroxide as adjuvant were not protected against S. mansoni infection.Centro de Estudos de Venenos e Animais Peçonhentos (CEVAP/UNESP)2009-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992009000200011Journal of Venomous Animals and Toxins including Tropical Diseases v.15 n.2 2009reponame:The Journal of venomous animals and toxins including tropical diseases (Online)instname:Universidade Estadual Paulista (UNESP)instacron:UNESP10.1590/S1678-91992009000200011info:eu-repo/semantics/openAccessMiyasato,PARamos,CRRAbreu,PAEDias,WONascimento,CHo,PLKawano,Teng2009-07-02T00:00:00Zoai:scielo:S1678-91992009000200011Revistahttp://www.scielo.br/jvatitdPUBhttps://old.scielo.br/oai/scielo-oai.php||editorial@jvat.org.br1678-91991678-9180opendoar:2009-07-02T00:00The Journal of venomous animals and toxins including tropical diseases (Online) - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Expression of schistosomal cathepsin l1 in Escherichia coli and evaluation of its protective capacity in an animal challenge |
title |
Expression of schistosomal cathepsin l1 in Escherichia coli and evaluation of its protective capacity in an animal challenge |
spellingShingle |
Expression of schistosomal cathepsin l1 in Escherichia coli and evaluation of its protective capacity in an animal challenge Miyasato,PA Schistosoma mansoni cysteine proteinase cathepsin L1 vaccine immunogenicity |
title_short |
Expression of schistosomal cathepsin l1 in Escherichia coli and evaluation of its protective capacity in an animal challenge |
title_full |
Expression of schistosomal cathepsin l1 in Escherichia coli and evaluation of its protective capacity in an animal challenge |
title_fullStr |
Expression of schistosomal cathepsin l1 in Escherichia coli and evaluation of its protective capacity in an animal challenge |
title_full_unstemmed |
Expression of schistosomal cathepsin l1 in Escherichia coli and evaluation of its protective capacity in an animal challenge |
title_sort |
Expression of schistosomal cathepsin l1 in Escherichia coli and evaluation of its protective capacity in an animal challenge |
author |
Miyasato,PA |
author_facet |
Miyasato,PA Ramos,CRR Abreu,PAE Dias,WO Nascimento,C Ho,PL Kawano,T |
author_role |
author |
author2 |
Ramos,CRR Abreu,PAE Dias,WO Nascimento,C Ho,PL Kawano,T |
author2_role |
author author author author author author |
dc.contributor.author.fl_str_mv |
Miyasato,PA Ramos,CRR Abreu,PAE Dias,WO Nascimento,C Ho,PL Kawano,T |
dc.subject.por.fl_str_mv |
Schistosoma mansoni cysteine proteinase cathepsin L1 vaccine immunogenicity |
topic |
Schistosoma mansoni cysteine proteinase cathepsin L1 vaccine immunogenicity |
description |
Schistosomes utilize proteinases to accomplish several activities such as tissue penetration, tissue digestion and evasion of host immune responses. Cathepsin L is a cysteine proteinase of the papain superfamily detected in their gut lumen which indicates that this enzyme contributes to the proteolysis of ingested hemoglobin. Due to the roles played in the schistosome biology, proteolytic enzymes are considered potential targets for developing and guiding antischistosomal therapies. In the present work, the cathepsin L1 cDNA coding of Schistosoma mansoni was cloned into the pAE vector that provides high-level expression of heterologous proteins in Escherichia coli. The recombinant protein was expressed as inclusion bodies, purified under denaturing conditions through nickel-charged chromatography and used for experimental animal vaccination. ELISA was performed with the pooled sera. Although this protein was shown to be immunogenic, mice immunized with three doses of recombinant protein plus aluminum hydroxide as adjuvant were not protected against S. mansoni infection. |
publishDate |
2009 |
dc.date.none.fl_str_mv |
2009-01-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992009000200011 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992009000200011 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S1678-91992009000200011 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Centro de Estudos de Venenos e Animais Peçonhentos (CEVAP/UNESP) |
publisher.none.fl_str_mv |
Centro de Estudos de Venenos e Animais Peçonhentos (CEVAP/UNESP) |
dc.source.none.fl_str_mv |
Journal of Venomous Animals and Toxins including Tropical Diseases v.15 n.2 2009 reponame:The Journal of venomous animals and toxins including tropical diseases (Online) instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
The Journal of venomous animals and toxins including tropical diseases (Online) |
collection |
The Journal of venomous animals and toxins including tropical diseases (Online) |
repository.name.fl_str_mv |
The Journal of venomous animals and toxins including tropical diseases (Online) - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
||editorial@jvat.org.br |
_version_ |
1748958538400530432 |