MICROBIOLOGICAL AND MOLECULAR EVALUATION OF Streptococcus equi SUBSPECIES equi AND RISK FACTORS ASSOCIATED TO EQUINE ADENITIS IN RURAL PROPERTIES OF RIO BRANCO MICRO-REGION, ACRE, BRAZIL
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Data de Publicação: | 2019 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Biota Amazônia |
Texto Completo: | https://periodicos.unifap.br/index.php/biota/article/view/4436 |
Resumo: | The objective of this study was to carry out a microbiological and molecular research of Streptococcus equi subspecies equi in the Rio Branco micro-region, Acre, Brazil, as well as to assess the risk factors. Sixty-four horses were submitted to clinical evaluation, from which nasal secretion samples were collected for bacteriological culture. The isolates were submitted to DNA extraction and PCR. The risk factors were verified through epidemiological questionnaires. It was verified that 32.8% (21/64) of the animals presented clinical signs suggestive. In the PCR, 50 (78.1%) samples were phenotypically compatible to genus, but none of them showed amplification of the 16SrRNA gene. The lack of disinfection of facilities and vaccination were the most frequent risk factors. It was concluded that Streptococcus equi subspecies equi is not present in the studied population, however, it may be susceptible to the disease due to the lack of sanitary criteria in the breeding.Keywords: Strangles; epidemiological study; Western Amazonia. |
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Biota Amazônia |
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MICROBIOLOGICAL AND MOLECULAR EVALUATION OF Streptococcus equi SUBSPECIES equi AND RISK FACTORS ASSOCIATED TO EQUINE ADENITIS IN RURAL PROPERTIES OF RIO BRANCO MICRO-REGION, ACRE, BRAZILAVALIAÇÃO MICROBIOLÓGICA E MOLECULAR DO Streptococcus equi SUBESPÉCIE equi E FATORES DE RISCO ASSOCIADOS À ADENITE EQUINA EM PROPRIEDADES RURAIS DA MICRORREGIÃO DE RIO BRANCO, ACRE, BRASILStrangles; epidemiological study; Western Amazonia.The objective of this study was to carry out a microbiological and molecular research of Streptococcus equi subspecies equi in the Rio Branco micro-region, Acre, Brazil, as well as to assess the risk factors. Sixty-four horses were submitted to clinical evaluation, from which nasal secretion samples were collected for bacteriological culture. The isolates were submitted to DNA extraction and PCR. The risk factors were verified through epidemiological questionnaires. It was verified that 32.8% (21/64) of the animals presented clinical signs suggestive. In the PCR, 50 (78.1%) samples were phenotypically compatible to genus, but none of them showed amplification of the 16SrRNA gene. The lack of disinfection of facilities and vaccination were the most frequent risk factors. It was concluded that Streptococcus equi subspecies equi is not present in the studied population, however, it may be susceptible to the disease due to the lack of sanitary criteria in the breeding.Keywords: Strangles; epidemiological study; Western Amazonia.Objetivou-se realizar uma pesquisa microbiológica e molecular do Streptococcus equi subesp. equi na microrregião de Rio Branco, Acre, bem como avaliar os fatores de risco. Foram submetidos à avaliação clínica 64 equinos, dos quais se colheu amostras de secreção nasal para cultivo bacteriológico. Os isolados foram encaminhados à extração de DNA e PCR. Os fatores de risco foram verificados por meio de questionários epidemiológicos. Verificou-se que 32,8% (21/64) dos animais apresentavam sinais clínicos sugestivos. Na PCR, 50 (78,1%) amostras foram compatíveis fenotipicamente ao gênero, mas nenhum destes apresentou amplificação do gene 16SrRNA. A ausência de desinfecção de instalações e vacinação foram os fatores de risco de maior frequência. Conclui-se que o Streptococcus equi subesp. equi não está presente na população estudada, porém, esta pode estar susceptível à doença, devido à falta de critérios sanitários nas criações.Palavras-chave: Garrotilho; estudo epidemiológico; Amazônia Ocidental.Universidade Federal do AmapáFundação de Amparo à Pesquisa do Estado do AcreGomes, Yuri AlmeidaReckziegel, Guilherme HenriqueBrito, Ingrid Danielle Santos de SouzaSilva, Tamyres Izarelly Barbosa da2019-09-30info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://periodicos.unifap.br/index.php/biota/article/view/443610.18561/2179-5746/biotaamazonia.v9n3p61-63Biota Amazônia (Biote Amazonie, Biota Amazonia, Amazonian Biota); v. 9, n. 3 (2019); 61-632179-5746reponame:Biota Amazôniainstname:Universidade Federal do Amapá (UNIFAP)instacron:UNIFAPenghttps://periodicos.unifap.br/index.php/biota/article/view/4436/v9n3p61-63.pdfWestern AmazoniaDireitos autorais 2019 Biota Amazônia (Biote Amazonie, Biota Amazonia, Amazonian Biota)http://creativecommons.org/licenses/by/4.0info:eu-repo/semantics/openAccess2019-12-26T13:28:05Zoai:ojs.pkp.sfu.ca:article/4436Revistahttp://periodicos.unifap.br/index.php/biotaONGhttps://periodicos.unifap.br/index.php/biota/oai||juliosa@unifap.br2179-57462179-5746opendoar:2019-12-26T13:28:05Biota Amazônia - Universidade Federal do Amapá (UNIFAP)false |
dc.title.none.fl_str_mv |
MICROBIOLOGICAL AND MOLECULAR EVALUATION OF Streptococcus equi SUBSPECIES equi AND RISK FACTORS ASSOCIATED TO EQUINE ADENITIS IN RURAL PROPERTIES OF RIO BRANCO MICRO-REGION, ACRE, BRAZIL AVALIAÇÃO MICROBIOLÓGICA E MOLECULAR DO Streptococcus equi SUBESPÉCIE equi E FATORES DE RISCO ASSOCIADOS À ADENITE EQUINA EM PROPRIEDADES RURAIS DA MICRORREGIÃO DE RIO BRANCO, ACRE, BRASIL |
title |
MICROBIOLOGICAL AND MOLECULAR EVALUATION OF Streptococcus equi SUBSPECIES equi AND RISK FACTORS ASSOCIATED TO EQUINE ADENITIS IN RURAL PROPERTIES OF RIO BRANCO MICRO-REGION, ACRE, BRAZIL |
spellingShingle |
MICROBIOLOGICAL AND MOLECULAR EVALUATION OF Streptococcus equi SUBSPECIES equi AND RISK FACTORS ASSOCIATED TO EQUINE ADENITIS IN RURAL PROPERTIES OF RIO BRANCO MICRO-REGION, ACRE, BRAZIL Gomes, Yuri Almeida Strangles; epidemiological study; Western Amazonia. |
title_short |
MICROBIOLOGICAL AND MOLECULAR EVALUATION OF Streptococcus equi SUBSPECIES equi AND RISK FACTORS ASSOCIATED TO EQUINE ADENITIS IN RURAL PROPERTIES OF RIO BRANCO MICRO-REGION, ACRE, BRAZIL |
title_full |
MICROBIOLOGICAL AND MOLECULAR EVALUATION OF Streptococcus equi SUBSPECIES equi AND RISK FACTORS ASSOCIATED TO EQUINE ADENITIS IN RURAL PROPERTIES OF RIO BRANCO MICRO-REGION, ACRE, BRAZIL |
title_fullStr |
MICROBIOLOGICAL AND MOLECULAR EVALUATION OF Streptococcus equi SUBSPECIES equi AND RISK FACTORS ASSOCIATED TO EQUINE ADENITIS IN RURAL PROPERTIES OF RIO BRANCO MICRO-REGION, ACRE, BRAZIL |
title_full_unstemmed |
MICROBIOLOGICAL AND MOLECULAR EVALUATION OF Streptococcus equi SUBSPECIES equi AND RISK FACTORS ASSOCIATED TO EQUINE ADENITIS IN RURAL PROPERTIES OF RIO BRANCO MICRO-REGION, ACRE, BRAZIL |
title_sort |
MICROBIOLOGICAL AND MOLECULAR EVALUATION OF Streptococcus equi SUBSPECIES equi AND RISK FACTORS ASSOCIATED TO EQUINE ADENITIS IN RURAL PROPERTIES OF RIO BRANCO MICRO-REGION, ACRE, BRAZIL |
author |
Gomes, Yuri Almeida |
author_facet |
Gomes, Yuri Almeida Reckziegel, Guilherme Henrique Brito, Ingrid Danielle Santos de Souza Silva, Tamyres Izarelly Barbosa da |
author_role |
author |
author2 |
Reckziegel, Guilherme Henrique Brito, Ingrid Danielle Santos de Souza Silva, Tamyres Izarelly Barbosa da |
author2_role |
author author author |
dc.contributor.none.fl_str_mv |
Fundação de Amparo à Pesquisa do Estado do Acre |
dc.contributor.author.fl_str_mv |
Gomes, Yuri Almeida Reckziegel, Guilherme Henrique Brito, Ingrid Danielle Santos de Souza Silva, Tamyres Izarelly Barbosa da |
dc.subject.none.fl_str_mv |
|
dc.subject.por.fl_str_mv |
Strangles; epidemiological study; Western Amazonia. |
topic |
Strangles; epidemiological study; Western Amazonia. |
description |
The objective of this study was to carry out a microbiological and molecular research of Streptococcus equi subspecies equi in the Rio Branco micro-region, Acre, Brazil, as well as to assess the risk factors. Sixty-four horses were submitted to clinical evaluation, from which nasal secretion samples were collected for bacteriological culture. The isolates were submitted to DNA extraction and PCR. The risk factors were verified through epidemiological questionnaires. It was verified that 32.8% (21/64) of the animals presented clinical signs suggestive. In the PCR, 50 (78.1%) samples were phenotypically compatible to genus, but none of them showed amplification of the 16SrRNA gene. The lack of disinfection of facilities and vaccination were the most frequent risk factors. It was concluded that Streptococcus equi subspecies equi is not present in the studied population, however, it may be susceptible to the disease due to the lack of sanitary criteria in the breeding.Keywords: Strangles; epidemiological study; Western Amazonia. |
publishDate |
2019 |
dc.date.none.fl_str_mv |
2019-09-30 |
dc.type.none.fl_str_mv |
|
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://periodicos.unifap.br/index.php/biota/article/view/4436 10.18561/2179-5746/biotaamazonia.v9n3p61-63 |
url |
https://periodicos.unifap.br/index.php/biota/article/view/4436 |
identifier_str_mv |
10.18561/2179-5746/biotaamazonia.v9n3p61-63 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
https://periodicos.unifap.br/index.php/biota/article/view/4436/v9n3p61-63.pdf |
dc.rights.driver.fl_str_mv |
Direitos autorais 2019 Biota Amazônia (Biote Amazonie, Biota Amazonia, Amazonian Biota) http://creativecommons.org/licenses/by/4.0 info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Direitos autorais 2019 Biota Amazônia (Biote Amazonie, Biota Amazonia, Amazonian Biota) http://creativecommons.org/licenses/by/4.0 |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.coverage.none.fl_str_mv |
Western Amazonia |
dc.publisher.none.fl_str_mv |
Universidade Federal do Amapá |
publisher.none.fl_str_mv |
Universidade Federal do Amapá |
dc.source.none.fl_str_mv |
Biota Amazônia (Biote Amazonie, Biota Amazonia, Amazonian Biota); v. 9, n. 3 (2019); 61-63 2179-5746 reponame:Biota Amazônia instname:Universidade Federal do Amapá (UNIFAP) instacron:UNIFAP |
instname_str |
Universidade Federal do Amapá (UNIFAP) |
instacron_str |
UNIFAP |
institution |
UNIFAP |
reponame_str |
Biota Amazônia |
collection |
Biota Amazônia |
repository.name.fl_str_mv |
Biota Amazônia - Universidade Federal do Amapá (UNIFAP) |
repository.mail.fl_str_mv |
||juliosa@unifap.br |
_version_ |
1800218371166306304 |