Toxicological study of seeds Moringa oleifera Lam. using the Artemia salina Leach test

Detalhes bibliográficos
Autor(a) principal: Dantas, Danilo Lima
Data de Publicação: 2020
Outros Autores: Sousa, Ana Paula Moisés de, Costa, Jaciara Dantas, Oliveira, José Anderson Machado, Almeida, Arthur Filgueira de, Apolinário, Marisa de Oliveira, Santana, Renato Alexandre Costa de, Campos, Ana Regina Nascimento
Tipo de documento: Artigo
Idioma: por
Título da fonte: Research, Society and Development
Texto Completo: https://rsdjournal.org/index.php/rsd/article/view/7332
Resumo: The search for plant species, especially those with the greatest economic and social benefits, has gained emphasis in scientific research. In this context, Moringa oleífera Lam. stands out, a plant originally from India and which has several applications in the food, industrial, medicinal, water treatment sectors, among others. Its fruits consist of pods that contain seeds rich in minerals, vitamins, proteins, lipids, carbohydrates, secondary metabolites and antioxidants. The present work had as objective to make a toxicological study of the seeds of M. oleifera Lam. using the Artemia salina Leach test. Two ethanolic extracts were prepared, one from almonds and the other with only the husks covering the seeds. The extracts were solubilized in 5 different concentrations. Each test tube contained the extracts in their respective concentrations and 10 active A. salina. After 24 h, the count was performed to determine the CL50. The data obtained were analyzed using PROBIT analysis using the Statistica software. From the analyzes performed, it was concluded that neither the almond nor the husk of the seed of M. oleifera Lam. presented toxicity, since both had CL50 values ​​above 1000 µg.mL-1, revealing even less toxicity than other plants conventionally used in Brazil, such as dill and fennel.
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spelling Toxicological study of seeds Moringa oleifera Lam. using the Artemia salina Leach testEstudio toxicológico de las semillas de Moringa oleifera Lam. utilizando la prueba de Artemia salina LeachEstudo toxicológico das sementes de Moringa oleifera Lam. utilizando o teste com Artemia salina LeachMoringaSementesToxicidade.MoringaSemillasToxicidad.MoringaSeedsToxicity.The search for plant species, especially those with the greatest economic and social benefits, has gained emphasis in scientific research. In this context, Moringa oleífera Lam. stands out, a plant originally from India and which has several applications in the food, industrial, medicinal, water treatment sectors, among others. Its fruits consist of pods that contain seeds rich in minerals, vitamins, proteins, lipids, carbohydrates, secondary metabolites and antioxidants. The present work had as objective to make a toxicological study of the seeds of M. oleifera Lam. using the Artemia salina Leach test. Two ethanolic extracts were prepared, one from almonds and the other with only the husks covering the seeds. The extracts were solubilized in 5 different concentrations. Each test tube contained the extracts in their respective concentrations and 10 active A. salina. After 24 h, the count was performed to determine the CL50. The data obtained were analyzed using PROBIT analysis using the Statistica software. From the analyzes performed, it was concluded that neither the almond nor the husk of the seed of M. oleifera Lam. presented toxicity, since both had CL50 values ​​above 1000 µg.mL-1, revealing even less toxicity than other plants conventionally used in Brazil, such as dill and fennel.La búsqueda de especies vegetales, especialmente aquellas con mayores beneficios económicos y sociales, ha ganado énfasis en la investigación científica. En este contexto, destaca Moringa oleífera Lam., Planta originaria de la India y que tiene varias aplicaciones en los sectores alimentario, industrial, medicinal, tratamiento de aguas, entre otros. Sus frutos consisten en vainas que contienen semillas ricas en minerales, vitaminas, proteínas, lípidos, carbohidratos, metabolitos secundarios y antioxidantes. El presente trabajo tuvo como objetivo realizar un estudio toxicológico de las semillas de M. oleifera Lam. Mediante el ensayo de lixiviación de Artemia salina. Se prepararon dos extractos etanólicos, uno de almendras y el otro con solo la cáscara cubriendo las semillas. Los extractos se solubilizaron en 5 concentraciones diferentes. Cada tubo de ensayo contenía los extractos en sus respectivas concentraciones y 10 A. solución salina activa. Después de 24 h, se realizó el recuento para determinar la CL50. Los datos obtenidos se analizaron mediante análisis PROBIT utilizando el software Statistica. De los análisis realizados se concluyó que ni la almendra ni la cáscara de la semilla de M. oleifera Lam. Presentaban toxicidad, ya que ambas tenían valores de CL50 superiores a 1000 µg.mL-1, revelando aún menos toxicidad que otras plantas convencionalmente utilizadas en Brasil, como el eneldo y el hinojo.A busca por espécies vegetais, sobretudo aquelas que apresentam maiores benefícios econômicos e também sociais vem ganhando ênfase nas pesquisas científicas. Nesse contexto destaca-se a Moringa oleífera Lam., planta originária da Índia e que apresenta diversas aplicações nos setores alimentícios, industriais, medicinais, tratamento de água, dentre outros. Seus frutos são constituídos de vagens que contêm sementes ricas em minerais, vitaminas, proteínas, lipídios, carboidratos, metabólitos secundários e antioxidantes. O presente trabalho teve por objetivo fazer um estudo toxicológico das sementes da M. oleifera Lam. a partir do teste da Artemia salina Leach. Foram preparados dois extratos etanólicos, sendo um a partir das amêndoas e outro apenas com as cascas que as recobrem. Os extratos foram solubilizados em 5 concentrações diferentes. Cada tubo de ensaio continha os extratos em suas respectivas concentrações e 10 A. salina ativas. Após 24 h foi realizada a contagem para determinação da CL50. Os dados obtidos foram analisados a partir de análise PROBIT com uso do software Statistica. A partir das análises realizadas concluiu-se que a nem a amêndoa e nem a casca da semente da M. oleifera Lam. apresentavam toxicidade, uma vez que ambas tiveram valores de CL50 acima de 1000 µg.mL-1, revelando inclusive uma menor toxicidade que outras plantas convencionalmente utilizadas no Brasil, como o endro e a erva doce.Research, Society and Development2020-08-26info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://rsdjournal.org/index.php/rsd/article/view/733210.33448/rsd-v9i9.7332Research, Society and Development; Vol. 9 No. 9; e457997332Research, Society and Development; Vol. 9 Núm. 9; e457997332Research, Society and Development; v. 9 n. 9; e4579973322525-3409reponame:Research, Society and Developmentinstname:Universidade Federal de Itajubá (UNIFEI)instacron:UNIFEIporhttps://rsdjournal.org/index.php/rsd/article/view/7332/6632Copyright (c) 2020 Danilo Lima Dantas; Ana Paula Moisés de Sousa; Jaciara Dantas Costa; José Anderson Machado Oliveira; Arthur Filgueira de Almeida; Marisa de Oliveira Apolinário; Renato Alexandre Costa de Santana; Ana Regina Nascimento Camposhttps://creativecommons.org/licenses/by/4.0info:eu-repo/semantics/openAccessDantas, Danilo Lima Sousa, Ana Paula Moisés de Costa, Jaciara Dantas Oliveira, José Anderson Machado Almeida, Arthur Filgueira de Apolinário, Marisa de Oliveira Santana, Renato Alexandre Costa de Campos, Ana Regina Nascimento 2020-09-18T01:42:11Zoai:ojs.pkp.sfu.ca:article/7332Revistahttps://rsdjournal.org/index.php/rsd/indexPUBhttps://rsdjournal.org/index.php/rsd/oairsd.articles@gmail.com2525-34092525-3409opendoar:2024-01-17T09:30:05.625983Research, Society and Development - Universidade Federal de Itajubá (UNIFEI)false
dc.title.none.fl_str_mv Toxicological study of seeds Moringa oleifera Lam. using the Artemia salina Leach test
Estudio toxicológico de las semillas de Moringa oleifera Lam. utilizando la prueba de Artemia salina Leach
Estudo toxicológico das sementes de Moringa oleifera Lam. utilizando o teste com Artemia salina Leach
title Toxicological study of seeds Moringa oleifera Lam. using the Artemia salina Leach test
spellingShingle Toxicological study of seeds Moringa oleifera Lam. using the Artemia salina Leach test
Dantas, Danilo Lima
Moringa
Sementes
Toxicidade.
Moringa
Semillas
Toxicidad.
Moringa
Seeds
Toxicity.
title_short Toxicological study of seeds Moringa oleifera Lam. using the Artemia salina Leach test
title_full Toxicological study of seeds Moringa oleifera Lam. using the Artemia salina Leach test
title_fullStr Toxicological study of seeds Moringa oleifera Lam. using the Artemia salina Leach test
title_full_unstemmed Toxicological study of seeds Moringa oleifera Lam. using the Artemia salina Leach test
title_sort Toxicological study of seeds Moringa oleifera Lam. using the Artemia salina Leach test
author Dantas, Danilo Lima
author_facet Dantas, Danilo Lima
Sousa, Ana Paula Moisés de
Costa, Jaciara Dantas
Oliveira, José Anderson Machado
Almeida, Arthur Filgueira de
Apolinário, Marisa de Oliveira
Santana, Renato Alexandre Costa de
Campos, Ana Regina Nascimento
author_role author
author2 Sousa, Ana Paula Moisés de
Costa, Jaciara Dantas
Oliveira, José Anderson Machado
Almeida, Arthur Filgueira de
Apolinário, Marisa de Oliveira
Santana, Renato Alexandre Costa de
Campos, Ana Regina Nascimento
author2_role author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Dantas, Danilo Lima
Sousa, Ana Paula Moisés de
Costa, Jaciara Dantas
Oliveira, José Anderson Machado
Almeida, Arthur Filgueira de
Apolinário, Marisa de Oliveira
Santana, Renato Alexandre Costa de
Campos, Ana Regina Nascimento
dc.subject.por.fl_str_mv Moringa
Sementes
Toxicidade.
Moringa
Semillas
Toxicidad.
Moringa
Seeds
Toxicity.
topic Moringa
Sementes
Toxicidade.
Moringa
Semillas
Toxicidad.
Moringa
Seeds
Toxicity.
description The search for plant species, especially those with the greatest economic and social benefits, has gained emphasis in scientific research. In this context, Moringa oleífera Lam. stands out, a plant originally from India and which has several applications in the food, industrial, medicinal, water treatment sectors, among others. Its fruits consist of pods that contain seeds rich in minerals, vitamins, proteins, lipids, carbohydrates, secondary metabolites and antioxidants. The present work had as objective to make a toxicological study of the seeds of M. oleifera Lam. using the Artemia salina Leach test. Two ethanolic extracts were prepared, one from almonds and the other with only the husks covering the seeds. The extracts were solubilized in 5 different concentrations. Each test tube contained the extracts in their respective concentrations and 10 active A. salina. After 24 h, the count was performed to determine the CL50. The data obtained were analyzed using PROBIT analysis using the Statistica software. From the analyzes performed, it was concluded that neither the almond nor the husk of the seed of M. oleifera Lam. presented toxicity, since both had CL50 values ​​above 1000 µg.mL-1, revealing even less toxicity than other plants conventionally used in Brazil, such as dill and fennel.
publishDate 2020
dc.date.none.fl_str_mv 2020-08-26
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://rsdjournal.org/index.php/rsd/article/view/7332
10.33448/rsd-v9i9.7332
url https://rsdjournal.org/index.php/rsd/article/view/7332
identifier_str_mv 10.33448/rsd-v9i9.7332
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dc.relation.none.fl_str_mv https://rsdjournal.org/index.php/rsd/article/view/7332/6632
dc.rights.driver.fl_str_mv https://creativecommons.org/licenses/by/4.0
info:eu-repo/semantics/openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/4.0
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Research, Society and Development
publisher.none.fl_str_mv Research, Society and Development
dc.source.none.fl_str_mv Research, Society and Development; Vol. 9 No. 9; e457997332
Research, Society and Development; Vol. 9 Núm. 9; e457997332
Research, Society and Development; v. 9 n. 9; e457997332
2525-3409
reponame:Research, Society and Development
instname:Universidade Federal de Itajubá (UNIFEI)
instacron:UNIFEI
instname_str Universidade Federal de Itajubá (UNIFEI)
instacron_str UNIFEI
institution UNIFEI
reponame_str Research, Society and Development
collection Research, Society and Development
repository.name.fl_str_mv Research, Society and Development - Universidade Federal de Itajubá (UNIFEI)
repository.mail.fl_str_mv rsd.articles@gmail.com
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